Fluorouracil (5-Fluoracil, 5-FU)

製品コードS1209 別名:NSC 19893

Fluorouracil (5-Fluoracil, 5-FU)化学構造


Fluorouracil (5-Fluoracil, 5-FU) is a DNA/RNA synthesis inhibitor, which interrupts nucleotide synthetic by inhibiting thymidylate synthase (TS) in tumor cells.

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JPY 22244.00
JPY 24402.00
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  • Quantitative RT-PCR analysis for mTOR levels in ASZ001 cells treated with ITRA, VISMO, or 5-FU. Data represent the mean ± SD of three independent experiments. * p < 0.05, compared to nontreated controls; ns, statistically not significant.

    J Invest Dermatol, 2018, 138(8):1716-1725. Fluorouracil (5-Fluoracil, 5-FU) purchased from Selleck.

    DNA-PKcs suppression mediated ROS production and GSH content in HepG2 cells exposed to CDDP and 5-Fu. a DNA-PKcs inhibition promoted ROS production in HepG2 cells treated with indicated concentrations of CDDP and 5-Fu. DCFH-DA fluorescent analysis was performed to assess the ROS level. Data presented were mean ?SD of three independent experiments.

    Mol Cell Biochem 2014 10.1007/s11010-014-2253-6. Fluorouracil (5-Fluoracil, 5-FU) purchased from Selleck.

  • EdU staining of RBE cells treated with OSI-027 (6.25 μM) and/or 5-FU (6.25 μg/mL) was performed by using Click-iT EdU Imaging Kit. The percentages of EdU-positive cells have been provided in the right panel.

    Eur Rev Med Pharmacol Sci, 2016, 20(9):1699-706.. Fluorouracil (5-Fluoracil, 5-FU) purchased from Selleck.


DNA/RNA Synthesis阻害剤の選択性比較


製品説明 Fluorouracil (5-Fluoracil, 5-FU) is a DNA/RNA synthesis inhibitor, which interrupts nucleotide synthetic by inhibiting thymidylate synthase (TS) in tumor cells.
Thymidylate synthase [1]
(Tumor cells)

Adrucil is an analogue of uracil with a fluorine atom at the C-5 position in place of hydrogen. It rapidly enters the cell using the same facilitated transport mechanism as uracil. Adrucil is converted intracellularly to several active metabolites: fluorodeoxyuridine monophosphate (FdUMP), fluorodeoxyuridine triphosphate (FdUTP) and fluorouridine triphosphate (FUTP). The Adrucil metabolite FdUMP binds to the nucleotide-binding site of TS, forming a stable ternary complex with the enzyme and CH2THF, thereby blocking binding of the normal substrate dUMP and inhibiting dTMP synthesis. Metabolite of Adrucil also can be misincorporated into DNA, leading to DNA strand breaks and cell death. The pro-apoptosis effects of Adrucil may be related to its activation of tumor suppressor p53. Loss of p53 function reduces cellular sensitivity to Adrucil. [1] Adrucil is able to inhibit the survival and induce apoptosis of a board range of cancer cells. Adrucil suppresses viabilities of the nasopharyngeal carcinoma cell line CNE2 and HONE1 [2], pancreatic cancer cell lines Capan-1 [3], and human colon carcinoma cell line HT-29 [4] with IC50 of 9 μg/mL, 3 μg/mL, 0.22 μM, 2.5 μM, respectively.

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF-7 MlnuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3rmOlczyqCqwrC= MULJR|UxRTJyIN88[{9uVA>? MnLSNlQxQTVzN{[=
HT-29 MmXTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYD6UHBSPzMEoHlCpC=> NXPTXW54UUN3ME6gNlUh|rypL33M M33DbFI1ODl3MUe2
HL-60 M4myNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUC3NuKhcMLi M1:zSmlEPTB;OD62NFEh|rypL33M NG\2fJIzPDB7NUG3Oi=>
NCI-H292 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17Z[|czyqCqwrC= M1TDRmlEPTB-IEK1JO69\y:vTB?= MUGyOFA6PTF5Nh?=


体内試験 Adrucil is widely used in the treatment of a range of cancers, including colorectal and breast cancers. [1] 100mg/kg Adrucil significantly suppresses tumor growth of murine colon carcinomas Colon 38 with tumor-doubling time (TD), growth-delay factor (GDF), and T/C of 26.5 days, 4.4, and 14%. [5]


細胞試験: [4]
+ 展開
  • 細胞株: Human colon carcinoma cell line HT-29
  • 濃度: ~25 μM
  • 反応時間: 7 days
  • 実験の流れ: Growth inhibition is measured after treatment of cells with Adrucil for 7 days in 96-well plates (4000 HT-29 cells/well in RPMI 1640 medium with 10% dialyzed fetal bovine serum); increasing concentrations of Adrucil are added after allowing for cell attachment overnight. At the end of incubation, cells are rinsed three times with phosphate-buffered saline (pH 7.4), fixed with 10% trichloroacetic acid for 60 min at 4 ℃, washed five times with deionized water, and stained with 0.4% sulforhoda-mine B solution for 15 min at room temperature. Unstained sulforhodamine B is removed by rinsing with 1% glacial acetic acid. Afterwards, stained cell proteins are dried and dissolved with 10 mM Tris-HCl. The optical density value is measured using a detector at 540 nm wavelength.
+ 展開
  • 動物モデル: Murine colon carcinomas Colon 38
  • 製剤: PBS
  • 投薬量: 100 mg/kg
  • 投与方法: i.p. weekly

溶解度 (25°C)

体外 DMSO 26 mg/mL (199.87 mM)
Water Insoluble
Ethanol Insoluble
体内 左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
saline (warming)

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。


分子量 130.08


CAS No. 51-21-8
in solvent
別名 NSC 19893





質量 (g) = 濃度 (mol/L) x 体積 (L) x 分子量 (g/mol)


  • 質量





開始濃度 x 開始体積 = 最終濃度 x 最終体積


この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 入力 出力 )

  • C1



  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):




チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2


質量 濃度 体積 分子量


NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03196180 Not yet recruiting High Grade Cervical Intraepithelial Neoplasia|p16 Positive Neoplastic Cells Present National Cancer Institute (NCI) February 28 2019 Phase 1
NCT03607643 Not yet recruiting Cancer of Pancreas|Cancer of Liver|Cancer of Rectum|Cancer of Colon|Cancer Gall Bladder|Myeloma Multiple|Glioblastoma Multiforme Leaf Vertical Inc. January 15 2019 Phase 1|Phase 2
NCT03698461 Not yet recruiting Colorectal Neoplasms|Neoplasm Metastasis|Colonic Neoplasms|Rectal Neoplasms Asan Medical Center|Hoffmann-La Roche December 2018 Phase 2
NCT03203525 Not yet recruiting Liver Cancer M.D. Anderson Cancer Center|NovoCure Ltd. December 2018 Phase 1
NCT03727074 Not yet recruiting Actinic Keratosis (AK) Sol-Gel Technologies Ltd. December 2018 Phase 3
NCT03721653 Not yet recruiting Metastatic Colorectal Cancer Gruppo Oncologico del Nord-Ovest|Roche Pharma AG November 15 2018 Phase 2



Handling Instructions


  • * 必須


  • 質問1:

    I was wondering if the product #s1209 (5-fluorouracil) is suitable to inject into mice ?

  • 回答:

    S1209 is suitable to inject (I.P.) into mice as indicating in this paper: http://www.ncbi.nlm.nih.gov/pubmed/8995503.

DNA/RNA Synthesisシグナル伝達経路

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細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID