Fluorouracil (5-Fluoracil, 5-FU)

製品コードS1209 別名:NSC 19893

Fluorouracil (5-Fluoracil, 5-FU)化学構造


Fluorouracil (5-Fluoracil, 5-FU) is a DNA/RNA synthesis inhibitor, which interrupts nucleotide synthetic by inhibiting thymidylate synthase (TS) in tumor cells.

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JPY 22244.00
JPY 24402.00
JPY 36520.00


  • DNA-PKcs suppression mediated ROS production and GSH content in HepG2 cells exposed to CDDP and 5-Fu. a DNA-PKcs inhibition promoted ROS production in HepG2 cells treated with indicated concentrations of CDDP and 5-Fu. DCFH-DA fluorescent analysis was performed to assess the ROS level. Data presented were mean ?SD of three independent experiments.

    Mol Cell Biochem 2014 10.1007/s11010-014-2253-6. Fluorouracil (5-Fluoracil, 5-FU) purchased from Selleck.

    EdU staining of RBE cells treated with OSI-027 (6.25 μM) and/or 5-FU (6.25 μg/mL) was performed by using Click-iT EdU Imaging Kit. The percentages of EdU-positive cells have been provided in the right panel.

    Eur Rev Med Pharmacol Sci, 2016, 20(9):1699-706.. Fluorouracil (5-Fluoracil, 5-FU) purchased from Selleck.


DNA/RNA Synthesis阻害剤の選択性比較


製品説明 Fluorouracil (5-Fluoracil, 5-FU) is a DNA/RNA synthesis inhibitor, which interrupts nucleotide synthetic by inhibiting thymidylate synthase (TS) in tumor cells.
Thymidylate synthase [1]
(Tumor cells)

Adrucil is an analogue of uracil with a fluorine atom at the C-5 position in place of hydrogen. It rapidly enters the cell using the same facilitated transport mechanism as uracil. Adrucil is converted intracellularly to several active metabolites: fluorodeoxyuridine monophosphate (FdUMP), fluorodeoxyuridine triphosphate (FdUTP) and fluorouridine triphosphate (FUTP). The Adrucil metabolite FdUMP binds to the nucleotide-binding site of TS, forming a stable ternary complex with the enzyme and CH2THF, thereby blocking binding of the normal substrate dUMP and inhibiting dTMP synthesis. Metabolite of Adrucil also can be misincorporated into DNA, leading to DNA strand breaks and cell death. The pro-apoptosis effects of Adrucil may be related to its activation of tumor suppressor p53. Loss of p53 function reduces cellular sensitivity to Adrucil. [1] Adrucil is able to inhibit the survival and induce apoptosis of a board range of cancer cells. Adrucil suppresses viabilities of the nasopharyngeal carcinoma cell line CNE2 and HONE1 [2], pancreatic cancer cell lines Capan-1 [3], and human colon carcinoma cell line HT-29 [4] with IC50 of 9 μg/mL, 3 μg/mL, 0.22 μM, 2.5 μM, respectively.

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF-7 NFfEcI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGq0Om84OsLiaNMg M3TP[WlEPTB;MkCg{txoN22O M3j2clI1ODl3MUe2
HT-29 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2LHU|czyqCqwrC= M1PmRmlEPTB-IEK1JO69\y:vTB?= MnO0NlQxQTVzN{[=
HL-60 M4PvZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGDXdXI4OsLiaNMg MkDkTWM2OD16Lk[wNUDPxGdxbVy= M372dlI1ODl3MUe2
NCI-H292 M4SycGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MojoO|LDqGkEoB?= M4TneGlEPTB-IEK1JO69\y:vTB?= Mn\4NlQxQTVzN{[=


体内試験 Adrucil is widely used in the treatment of a range of cancers, including colorectal and breast cancers. [1] 100mg/kg Adrucil significantly suppresses tumor growth of murine colon carcinomas Colon 38 with tumor-doubling time (TD), growth-delay factor (GDF), and T/C of 26.5 days, 4.4, and 14%. [5]


細胞試験: [4]
+ 展開
  • 細胞株: Human colon carcinoma cell line HT-29
  • 濃度: ~25 μM
  • 反応時間: 7 days
  • 実験の流れ: Growth inhibition is measured after treatment of cells with Adrucil for 7 days in 96-well plates (4000 HT-29 cells/well in RPMI 1640 medium with 10% dialyzed fetal bovine serum); increasing concentrations of Adrucil are added after allowing for cell attachment overnight. At the end of incubation, cells are rinsed three times with phosphate-buffered saline (pH 7.4), fixed with 10% trichloroacetic acid for 60 min at 4 ℃, washed five times with deionized water, and stained with 0.4% sulforhoda-mine B solution for 15 min at room temperature. Unstained sulforhodamine B is removed by rinsing with 1% glacial acetic acid. Afterwards, stained cell proteins are dried and dissolved with 10 mM Tris-HCl. The optical density value is measured using a detector at 540 nm wavelength.
+ 展開
  • 動物モデル: Murine colon carcinomas Colon 38
  • 製剤: PBS
  • 投薬量: 100 mg/kg
  • 投与方法: i.p. weekly

溶解度 (25°C)

体外 DMSO 26 mg/mL (199.87 mM)
Water Insoluble
Ethanol Insoluble
体内 左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
saline (warming)

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。


分子量 130.08


CAS No. 51-21-8
in solvent
別名 NSC 19893





質量 (g) = 濃度 (mol/L) x 体積 (L) x 分子量 (g/mol)


  • 質量





開始濃度 x 開始体積 = 最終濃度 x 最終体積


この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 入力 出力 )

  • C1



  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):




チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2


質量 濃度 体積 分子量


NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03485196 Recruiting Gastric Cancer Affiliated Hospital of Qinghai University|the Second Hospital of Lanzhou University|Qinghai People''s Hospital April 8 2018 --
NCT03099486 Recruiting Colorectal Cancer Fox Chase Cancer Center October 6 2017 Phase 2
NCT02344810 Withdrawn Adenocarcinoma of the Esophagus|Adenocarcinoma of the Gastroesophageal Junction|Diffuse Adenocarcinoma of the Stomach|Gastrointestinal Cancer|Intestinal Adenocarcinoma of the Stomach|Mixed Adenocarcinoma of the Stomach|Stage IIIA Esophageal Cancer|Stage IIIA Gastric Cancer|Stage IIIB Esophageal Cancer|Stage IIIB Gastric Cancer|Stage IIIC Esophageal Cancer|Stage IIIC Gastric Cancer|Stage IV Esophageal Cancer|Stage IV Gastric Cancer Eastern Cooperative Oncology Group|National Cancer Institute (NCI)|ECOG-ACRIN Cancer Research Group March 6 2015 Phase 1|Phase 2
NCT02648425 Completed Solid Tumor Aslan Pharmaceuticals August 5 2014 Phase 1
NCT03524508 Recruiting Metastatic Biliary Tract Cancer Changhoon Yoo|Ulsan University Hospital|Chungnam National University Hospital|Kyungpook national university Chilgok hospital|Inje University|Asan Medical Center September 4 2018 Phase 2
NCT03210064 Recruiting Metastatic Colorectal Cancer Hui ting Xu,MD|Jiangsu HengRui Medicine Co. Ltd.|Hubei Cancer Hospital May 4 2017 Phase 2



Handling Instructions


  • * 必須


  • 質問1:

    I was wondering if the product #s1209 (5-fluorouracil) is suitable to inject into mice ?

  • 回答:

    S1209 is suitable to inject (I.P.) into mice as indicating in this paper: http://www.ncbi.nlm.nih.gov/pubmed/8995503.

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細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID