Gemcitabine (LY-188011) HCl

製品コード:S1149 別名:NSC 613327

For research use only.

Gemcitabine (LY-188011, NSC 613327) HCl is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.

Gemcitabine (LY-188011) HCl化学構造

CAS No. 122111-03-9

サイズ 価格(税別)
JPY 13000
JPY 17400
JPY 36800

代表番号: 03-5615-9297|電子メール:[email protected]
よく尋ねられる質問

文献中Selleckの製品使用例(144)

製品安全説明書

DNA/RNA Synthesis阻害剤の選択性比較

相関DNA/RNA Synthesis製品

生物活性

製品説明 Gemcitabine (LY-188011, NSC 613327) HCl is a DNA synthesis inhibitor with IC50 of 50 nM, 40 nM, 18 nM and 12 nM in PANC1, MIAPaCa2, BxPC3 and Capan2 cells, respectively.
特性 Gemcitabine has been used to treat pancreatic cancer and has demonstrated effective anti-tumor activity.
ターゲット
DNA synthesis (Capan2 cells) [1] DNA synthesis (BxPC3 cells) [1] DNA synthesis (MIAPaCa2 cells) [1] DNA synthesis (PANC1 cells) [1]
12 nM 18 nM 40 nM 50 nM
体外試験

Gemcitabine induced NF-κB activity in BxPC-3, PANC-1, and MIA PaCa-2 cells and decreased the level of the NF-κB inhibitor IκBα in BxPC-3 and PANC-1 cells. Treatment of BxPC-3 cells with low dose Gemcitabine for 48 hours results in a dose-dependent increase in NF-κB binding. In contrast, NF-κB DNA binding is decreased in BxPC-3 cells treated with the higher Gemcitabine doses for 48 h; however, 24-h treatment with these higher doses increases NF-κB binding in BxPC-3 cells [2]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CCRF-CEM M{Pybmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoSwTWM2OD1{LkmgxtEhOS56IH7N NHSwfWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
CCRF-CEM/dCK−/− NVzGd2diT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4TLeGlEPTB;MkSwMlQhyrFiMkmuNEDPxE1? NFX0V5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
L1210 wt NXO4VIJsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTFwMzFCtUAxNjNibl2= MmDwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2MkW4PFUoRjJ{NEK1PFg2RC:jPh?=
L1210 10K NX2x[ZVCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTJ{LkKgxtEhOy55IN88US=> NVf6XHpORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
TC-1  MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWTZRWNuUUN3ME2xOE44KMLzIEKuPEBvVQ>? NVfkWlRrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
TC-1-GR Mm\LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTjT2I{UUN3ME2zOk44KMLzIEWuNUDPxE1? NF;XbVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
MIA PaCa-2 NHLue5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFrqfIVKSzVyPUS5MlchyrFiMUeuO{BvVQ>? NVTrUYhmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
PANC-1 MmrSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnPkTWM2OD1-IESwNEDPxE1? NInUPHQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
CCRF-CEM MlfhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2rtVWlEPTB;Mj65JOKyKDFwODDuUS=> M1\sTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
CCRF-CEM-AraC-8C M1PuV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjITWM2OD17OUiuPEDDuSB7LkSgcm0> NF;nNJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
CCRF-CEM  NX70RXhWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jabFczKGh? MnLDTWM2OD1{LkCgxtEhOC54IH7N NEPhU|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi1NVg1Oyd-MkG4OVE5PDN:L3G+
MV-4-11 M1roV2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmPrTWM2OCB;IECuNFAxPSCwTR?= M2LhRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
ES4 MkHXS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NH:xW2tKSzVyIE2gNE4xODB5IH7N NF;pSIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
ACHN M{PZWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYTJR|UxKD1iMD6wNFA6KG6P Mn7kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
KYSE-510 MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWPJR|UxKD1iMD6wNFEhdk1? M4rZPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
EW-7 MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1qzdGlEPTBiPTCwMlAxOjZibl2= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
BFTC-905 MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGTQXFRKSzVyIE2gNE4xODVzIH7N M4PEb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
KE-37 MXHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1fHT2lEPTBiPTCwMlAxPTZibl2= NH;NOoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
SBC-5 MlHWS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{HiRmlEPTBiPTCwMlAxPTdibl2= NYjsU25lRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
NKM-1 NYHlRXJVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVfJR|UxKD1iMD6wNFcyKG6P Mkn2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
RH-1 MkP1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEDRbFRKSzVyIE2gNE4xODd{IH7N M4nabFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
ALL-PO MkCzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYjEe3ZUUUN3MDC9JFAvODB6MzDuUS=> M4LrRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
QIMR-WIL NYiwdXo{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4HtXWlEPTBiPTCwMlAxQDlibl2= NEXxZVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
A375 MlTES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MY\JR|UxKD1iMD6wNFk6KG6P MmLUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
SIG-M5 M3fyWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGXOT5lKSzVyIE2gNE4xOTB2IH7N NWT3XJFsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
KGN NH;LeGJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn;uTWM2OCB;IECuNFExQCCwTR?= NWPnNos{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
EW-13 MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYTzVGE{UUN3MDC9JFAvODFzMjDuUS=> MoS5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
NCI-SNU-1 MmH4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYnSTpBCUUN3MDC9JFAvODF4IH7N MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
PSN1 MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVjJR|UxKD1iMD6wNVY2KG6P NUji[oRORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
HUTU-80 MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUHJR|UxKD1iMD6wNVY3KG6P M2\NXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
786-0 MYPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1z1XWlEPTBiPTCwMlAzOyCwTR?= NWDMW5p2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
EW-16 MnLIS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWLVS4JZUUN3MDC9JFAvODJ|IH7N NWH2dVVRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
ES1 Ml3pS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MV3JR|UxKD1iMD6wNlY5KG6P MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
RKO M4TCTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWPNWppXUUN3MDC9JFAvODJ5ODDuUS=> NV6xUZRrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
ESS-1 M3Pr[2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoXGTWM2OCB;IECuNFI5PiCwTR?= M{\RN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
SK-UT-1 MnLWS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVTJR|UxKD1iMD6wNlk4KG6P MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
LB2241-RCC MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYrXd2lpUUN3MDC9JFAvODNzODDuUS=> NInHZ2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
CHL-1 NFrpd5BIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2DUbGlEPTBiPTCwMlA{OjRibl2= NHPseWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
SW1783 Mnv3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGnyfZBKSzVyIE2gNE4xOzN4IH7N M4\ZNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
MEL-JUSO NWLJS5Y5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUTEe2lDUUN3MDC9JFAvODN7MTDuUS=> NV[wWXNmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
HT-29 NH;HNGRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHTnXmpKSzVyIE2gNE4xPDF|IH7N M2f1UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
SNG-M MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXPJR|UxKD1iMD6wOFI2KG6P NGTXe|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
TE-15 M1LmOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHLZR2tKSzVyIE2gNE4xPDZ2IH7N MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
HOS NGHsbGJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXLJR|UxKD1iMD6wOFghdk1? MlPGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
BB65-RCC NVfG[W1MT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MX\JR|UxKD1iMD6wOVEzKG6P M3XLeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
HCE-4 MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NE\qT3lKSzVyIE2gNE4xPTJ6IH7N NHTKWoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
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MIAPaCa2 Mn3tR5l1d3SxeHnjbZR6KGG|c3H5 M{K1XFczKGi{cx?= MXrJR|UxKD1iMT6wOEDPxE1? NHPLSYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES3NVk6QCd-MkS0O|E6QTh:L3G+
SK-N-AS NH7SXllEgXSxdH;4bYNqfHliYYPzZZk> MnGxN{Bl[Xm| M4DBT2lEPTBiPTCxMlEh|ryP M3vEdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
SW1990 NWPWTo82S3m2b4TvfIlkcXS7IHHzd4F6 NGTtRpc4OiCqcoO= NWPqUohsUUN3MDC9JFEvOiEQvF2= MnXwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|NkK0O|QoRjF7M{[yOFc1RC:jPh?=
A549 M16yO2N6fG:2b4jpZ4l1gSCjc4PhfS=> Mn\QO|IhcHK| MkSwTWM2OCB;IEGuOEDPxE1? MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN4MkS3OEc,OTl|NkK0O|Q9N2F-
HepG2 M{ixbmZ2dmO2aX;uJIF{e2G7 M{Dqb2tuKD1iMT60JO69VQ>? MkLsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzMEG2O|QoRjF5MUCxOlc1RC:jPh?=
BL21(DE3) MWPGeY5kfGmxbjDhd5NigQ>? NX;YOVNxU21iPTCxMlQ{KM7:TR?= MkL2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{M{CxN|EoRjJ|MkOwNVMyRC:jPh?=
U87MG MV\DfZRwfG:6aXPpeJkh[XO|YYm= MW[zJIRigXN? NFvNc2lKSzVyIE2gNU41QSEQvF2= NFzhXFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUexNVA2PCd-MkG3NVExPTR:L3G+
BL21(DE3) Mn;xSpVv[3Srb36gZZN{[Xl? NELQTJdMdSB;IEGuOVIh|ryP M3\QRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkOwNVMyLz5{M{KzNFE{OTxxYU6=
HT-29 NFrUWG5EgXSxdH;4bYNqfHliYYPzZZk> NGTkVFg{KGSjeYO= NHLGZYhKSzVyIE2gNU42OyEQvF2= MkDSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MUGwOVQoRjJzN{GxNFU1RC:jPh?=
SW1990 NE\6[plEgXSxdH;4bYNqfHliYYPzZZk> NWDvdJR[UUN3MDC9JFEvPiEQvF2= NHzlfJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG5OVQ3Pid-MkSxPVU1PjZ:L3G+
Capan2 NHuxUWZEgXSxdH;4bYNqfHliYYPzZZk> MkXYO|IhcHK| M1nlNWlEPTBiPTCxMlch|ryP NHnLd5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO2NlQ4PCd-MUmzOlI1PzR:L3G+
SW480 MW\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M{XQS|czKGi{cx?= NF\3eXFKSzVyIE2gNU44KM7:TR?= NEnrS5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi3N|c1OCd-MkC4O|M4PDB:L3G+
PANC1 NWHDPY1iS3m2b4TvfIlkcXS7IHHzd4F6 M{fpSlczKGi{cx?= MVvJR|UxKD1iMT63JO69VQ>? Mn\XQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4NU[yNFIoRjJ7NkW2NlAzRC:jPh?=
HCT8 Ml34R5l1d3SxeHnjbZR6KGG|c3H5 MnzaO|IhcHK| NHi1VlZKSzVyIE2gNU44PCEQvF2= M{HtU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
BL21(DE3) M2nZR2Z2dmO2aX;uJIF{e2G7 M4\3emtuKD1iMT63OUDPxE1? MmL4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{M{CxN|EoRjJ|MkOwNVMyRC:jPh?=
HT-29 NGXMT4VEgXSxdH;4bYNqfHliYYPzZZk> M1v1flQ5KGi{cx?= M2DiZmlEPTBiPTCxMlk2KM7:TR?= NHHlenI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2PFgzPCd-MkO5Olg5OjR:L3G+
MIAPaCa2 M4fibWN6fG:2b4jpZ4l1gSCjc4PhfS=> NYPkPYJmPSCmYYnz NIDYNHdGSzVyIE2gNkDPxE1? Mnr0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6Nke1PVAoRjJ2OE[3OVkxRC:jPh?=
BL21(DE3) NH3lTGFHfW6ldHnvckBie3OjeR?= MoX3NUBucW5? NWPnSmhqU21iPTCyMlE2KM7:TR?= M1q1N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkOwNVMyLz5{M{KzNFE{OTxxYU6=
SW1990 M2nifmN6fG:2b4jpZ4l1gSCjc4PhfS=> MkDVTWM2OCB;IEKuNkDPxE1? NYfVOGZrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNFU4OjJpPkK1NVA2PzJ{PD;hQi=>
SW1990 MnztR5l1d3SxeHnjbZR6KGG|c3H5 M4\tNFQ5KGi{cx?= NWHQc4hTUUN3MDC9JFIvOyEQvF2= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzl4Nkm1NEc,Ojd7Nk[5OVA9N2F-
NCI-H146 MoTtR5l1d3SxeHnjbZR6KGG|c3H5 MXqzJIRigXN? NHXOZVVKSzVyIE2gNk44QCEQvF2= NEfhO209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUexNVA2PCd-MkG3NVExPTR:L3G+
BxPC3 NVrwZlVkS3m2b4TvfIlkcXS7IHHzd4F6 NWPQTpc6PzJiaILz NF:zXGNKSzVyIE2gNk46KM7:TR?= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN4MkS3OEc,OTl|NkK0O|Q9N2F-
p53 deficient COLO205 NE\vbVBCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NHLGclU4OiCqcoO= NICyXZhKSzVyIE2gN{DPxE1? M1zldlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOEezO|QxLz5{MEi3N|c1ODxxYU6=
MiaPaCa MmjsR5l1d3SxeHnjbZR6KGG|c3H5 NY\OXWtQPiCmYYnz M4PkWGlEPTBiPTCzJO69VQ>? NUnudHoyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzOlAyODRpPkKzN|YxOTB2PD;hQi=>
COLO205 MUTDfZRwfG:6aXPpeJkh[XO|YYm= M4LkPFQ5KGi{cx?= NY[1N444UUN3MDC9JFMvOjRizszN NVrTTmxnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5Olg5OjRpPkKzPVY5QDJ2PD;hQi=>
MCF7 MXrDfZRwfG:6aXPpeJkh[XO|YYm= NIq5UHc4OiCqcoO= MXvJR|UxKD1iMz6yPEDPxE1? NYnqW5Z4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzOlI1PzRpPkG5N|YzPDd2PD;hQi=>
MIAPaCa2 M1[5fmN6fG:2b4jpZ4l1gSCjc4PhfS=> MnLYO|IhcHK| MUfJR|UxKD1iMz6zJO69VQ>? NXrIOFA5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmzNlg3PTZpPkK5N|I5PjV4PD;hQi=>
HeLa NFvHZVNEgXSxdH;4bYNqfHliYYPzZZk> MVGyOEBpenN? Mm[5TWM2OCB;IEOuN{DPxE1? NUTM[olORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3NFMzQTZpPkK1O|A{Ojl4PD;hQi=>
FTC-133 NXfZcXNqS3m2b4TvfIlkcXS7IHHzd4F6 MYOyOEBpenN? M2ft[2lEPTBiPTCzMlM3KM7:TR?= M1XjVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEO2PVk1Lz5{NESzOlk6PDxxYU6=
BxPC3 NILOblNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVLSenJYPDhiaILz M1yzcmdKPTBiPTCzMlY1KM7:TR?= M3jsUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUGyPVA5Lz5{MkWxNlkxQDxxYU6=
COLO320DM MkHtR5l1d3SxeHnjbZR6KGG|c3H5 NUjMOodKPDhiaILz MVzJR|UxKD1iMz65NkDPxE1? NYjL[JlIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5Olg5OjRpPkKzPVY5QDJ2PD;hQi=>
HeLa M1frRWN6fG:2b4jpZ4l1gSCjc4PhfS=> M3zLdlMh\GG7cx?= M4XPSGlEPTBiPTC0MlEzKM7:TR?= NXztW|V{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3NVExPTRpPkKxO|EyODV2PD;hQi=>
8305C Mn\ZR5l1d3SxeHnjbZR6KGG|c3H5 NH\GXmYzPCCqcoO= NVfFNpZLUUN3MDC9JFQvPTNizszN MkLJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2M{[5PVQoRjJ2NEO2PVk1RC:jPh?=
PANC1 MY\DfZRwfG:6aXPpeJkh[XO|YYm= MmHBO|IhcHK| MoX4TWM2OCB;IEWuOkDPxE1? NV\mWVNXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzOlI1PzRpPkG5N|YzPDd2PD;hQi=>
PANC1 MkiwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXXHO|htPzJiaILz MXLHTVUxKD1iNT64JO69VQ>? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR7NUC4NUc,Ojh2OUWwPFE9N2F-
SK-MEL-2 M2\Id2N6fG:2b4jpZ4l1gSCjc4PhfS=> NFTrTGs{KGSjeYO= NEjxb3VKSzVyIE2gO{4yOSEQvF2= NFLESYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUexNVA2PCd-MkG3NVExPTR:L3G+
CEM MoTjR5l1d3SxeHnjbZR6KGG|c3H5 MWKzJIRigXN? NF\hXW5KSzVyIE2gO{43KM7:TR?= MmD5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NEGzOVYoRjJ2M{SxN|U3RC:jPh?=
SW1573 MX7DfZRwfG:6aXPpeJkh[XO|YYm= NVHzXpNPPzJiaILz MYTJR|UxKD1iOD6zJO69VQ>? M{jn[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEG5OlA1Lz5zN{SxPVYxPDxxYU6=
BxPC3 NVjjdYpjT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUjjV3EyPDhiaILz MXzMR|UxKD1iOD63NUDPxE1? NFPkenM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWxNlkxQCd-MkK1NVI6ODh:L3G+
MCF7 NVP3RmdtS3m2b4TvfIlkcXS7IHHzd4F6 NX32XJIxPzJiaILz NVLSTpFpUUN3MDC9JFgvQSEQvF2= MlXjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyN{W1PVIoRjJ6MEe1OVkzRC:jPh?=
BJ NUjBXoQzS3m2b4TvfIlkcXS7IHHzd4F6 NYHFboZ3OyCmYYnz MYnJR|UxKD1iOT64PEDPxE1? MlfUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MUGwOVQoRjJzN{GxNFU1RC:jPh?=
HeLa Mk\JR5l1d3SxeHnjbZR6KGG|c3H5 MVW3NkBpenN? MYTJR|UxKD1iMUCg{txO MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ|MUO1PUc,OjR4M{GzOVk9N2F-
B16-F10-Luc-G5 MnfRRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M1L0bVI1KGi{cx?= NFnFZnZKSzVyIE2gNVEh|ryP NFLDOYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{O0PVM{Oid-MkezOFk{OzJ:L3G+
HCT15 M4PBU2N6fG:2b4jpZ4l1gSCjc4PhfS=> MVrJR|UxKD1iMUGuPEDPxE1? M2THWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MUi2OlA1Lz5zOEG4OlYxPDxxYU6=
A549 MUTDfZRwfG:6aXPpeJkh[XO|YYm= M164SlczKGi{cx?= NX3EO|Y5UUN3MDC9JFE{NjFizszN NIPxU|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{SxPVYxPCd-MUe0NVk3ODR:L3G+
HCT116 M1f1c2N6fG:2b4jpZ4l1gSCjc4PhfS=> MUDJR|UxKD1iMUSuN{DPxE1? NEXUO2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEG4OlYxPCd-MUixPFY3ODR:L3G+
MiaPaCa MoX3R5l1d3SxeHnjbZR6KGG|c3H5 MkC2O|IhcHK| NH7qc3FKSzVyIE2gNVcvOSEQvF2= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR6OU[yOkc,OjN2OEm2NlY9N2F-
AG6000 MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mm\uTWM2OCB;IEKwJO69VQ>? MlXOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4MEK0OlQoRjF5NkCyOFY1RC:jPh?=
AG6000 NYTofFU{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1;PXWlEPTBiPTCyNEDPxE1? M1\YOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkCyOFY1Lz5zN{[wNlQ3PDxxYU6=
BxPC3 NGC3WpFCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NGe0c|E4OiCqcoO= M{TlZ2lEPTBiPTCyNE4zKM7:TR?= M1;adFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUe2OlM{Lz5{OEW3OlY{OzxxYU6=
CV1 MX\DfZRwfG:6aXPpeJkh[XO|YYm= NWXBRmpXPzJiaILz MYrJR|UxKD1iMkGuPUDPxE1? NV3IZ242RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PFk3OjZpPkKzOFg6PjJ4PD;hQi=>
AsPC1 MYLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NFXLZ|U4OiCqcoO= NWfDcVNXUUN3MDC9JFI3NjhizszN NXXXTIx5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1O|Y3OzNpPkK4OVc3PjN|PD;hQi=>
PANC1 MlrQRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M{LBXFczKGi{cx?= NULNTlRLUUN3MDC9JFMxNjRizszN MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV5Nk[zN{c,Ojh3N{[2N|M9N2F-
MIAPaCa2 M3m2WGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{HSc|Q5KGi{cx?= M2GwUmdKPTBiPTCzOU45OyEQvF2= MmLKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3MUK5NFgoRjJ{NUGyPVA5RC:jPh?=
アッセイ
Methods Test Index PMID
Western blot p-JNK / JNK / p-ATF2 / p-c-Jun ; caspase-3 / caspase-8 / caspase-9 ; pS-ERα / ERα / pERK / ERK ; LC3-I / LC3-II ; p70S6K1 / p-S6 / HIF-1α 16307741 27384485 27765914
Immunofluorescence BMI1 27177084
Growth inhibition assay Cell viability 27765914
体内試験 Intratumoral NF-κB activity is significantly elevated (1.3- to 1.8-fold) in the Gemcitabine-treated mice compared to the PBS-treated mice, suggesting that Gemcitabine also induces NF-κB activation. [2]

お薦めの試験操作(参考用のみ)

細胞試験:[2]
  • 細胞株:BxPC-3, MIA PaCa-2, and PANC-1 cells
  • 濃度: 0.2 μM
  • 反応時間:24 hours or 48 hours
  • 実験の流れ:BxPC-3, MIA PaCa-2, and PANC-1 cells are seeded in a 96-well plate. After 24 hours, cells are treated with vehicle, DMAPT and/or Gemcitabine for an additional 24 hours or 48 hours. Apoptosis is quantified using the Cell Death Detection ELISA to detect the amount of cytoplasmic histone-associated DNA fragments and expressed relative to vehicle-treated cells.
動物試験:[2]
  • 動物モデル:Athymic nude mice with MIA PaCa-2 cells
  • 投薬量:50 mg/kg or 100 mg/kg
  • 投与方法:Administered via i.p.

溶解度 (25°C)

体外

体内

左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
Saline
混合させたのち直ちに使用することを推奨します。

19mg/mL

化学情報

分子量 299.66
化学式

C9H11F2N3O4.HCI

CAS No. 122111-03-9
Storage 3年 -20°C
2年 -80°C in solvent
Smiles C1=CN(C(=O)N=C1N)C2C(C(C(O2)CO)O)(F)F.Cl

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

便利ツール

質量 濃度 体積 分子量

臨床試験

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05141149 Not yet recruiting Drug: PBP1510 (400mg/16mL)|Drug: Gemcitabine (1000 mg/m^2) Pancreatic Cancer Prestige Biopharma Limited July 2022 Phase 1|Phase 2
NCT05181462 Recruiting Drug: Carboplatin|Drug: Gemcitabine|Drug: Nadunolimab Triple Negative Breast Cancer Cantargia AB|Spanish Breast Cancer Research Group January 11 2022 Phase 1|Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

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お問い合わせ内容をご入力ください

よくある質問(FAQ)

質問1:
What’s the difference between S1714 and S1149 and which one is better?

回答
They have the same biological activities. The free base(S1714) dissolves better in DMSO, and the hydrochloride (S1149) dissolves better in water.

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