Tamoxifen (ICI 46474)

製品コード:S1238 別名:(Z)-Tamoxifen, trans-Tamoxifen

For research use only.

Tamoxifen (ICI 46474, (Z)-Tamoxifen, trans-Tamoxifen) is a selective estrogen receptor modulator (SERM). Tamoxifen enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen induces apoptosis.

Tamoxifen (ICI 46474)化学構造

CAS No. 10540-29-1

サイズ 価格(税別)
10mM (1mL in DMSO) JPY 12000
JPY 13300
JPY 19100

代表番号: 03-5615-9297|電子メール:[email protected]
よく尋ねられる質問

文献中Selleckの製品使用例(43)

製品安全説明書

Antineoplastic and Immunosuppressive Antibiotics阻害剤の選択性比較

生物活性

製品説明 Tamoxifen (ICI 46474, (Z)-Tamoxifen, trans-Tamoxifen) is a selective estrogen receptor modulator (SERM). Tamoxifen enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen induces apoptosis.
ターゲット
estrogen receptor [1]
体外試験

TAM treatment inhibits significantly MCF7 cell proliferation. Low doses of TAM are able to induce structural chromosomal aberrations (deletions, isochromosomes, translocations, and dicentric chromosomes) in both ER+ and ER- breast cancer cells. This genotoxic effect is higher in those cell lines with HER2 gene amplification[2]. Whereas TAM at lower concentrations (0.1-1 μM) induces a cell-cycle arrest, pharmacological concentrations (above 5 μM) of TAM have been found to induce apoptosis of breast cancer cells. 5 μM TAM rapidly induced sustained activation of ERK1/2 in ER-positive breast cancer cell lines (MCF-7 and T47D)[3]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MDA-MB-435 cells M3\zR2Z2dmO2aX;uJIF{e2G7 NEHHUlZKdmirYnn0bY9vKG:oIF3ERU1OSi12M{WgZpJm[XO2IHPhcoNmeiClZXzsJJBzd2yrZnXyZZRqd25uIFnDOVA:OC5yNTFOwG0> M2q4[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2OUexPFk6Lz5zNEm3NVg6QTxxYU6=
MDA-MB-231 cells M{\QeWZ2dmO2aX;uJIF{e2G7 M1[zVmlvcGmkaYTpc44hd2ZiZYP0do9o\W5icnXj[ZB1d3JibnXnZZRqfmViTVTBMW1DNTJ|MTDidoVie3RiY3HuZ4VzKGOnbHygdJJwdGmoZYLheIlwdixiSVO1NF0xNjB6IN88US=> NHHCUHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNEm3NVg6QSd-MUS5O|E5QTl:L3G+
MCF7 NFLYWZlCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MUC3NkBpenN? M{jMOWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgUWNHPyClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBC[3Srdnn0fUA:KDRwNjFOwG0v NHjXWXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{mxPFgzOCd-MUe5NVg5OjB:L3G+
MCF7 NUTqSIl1SW62aXXzeJJw\2WwaXOgZZN{[Xl? M3HH[Fk3KGi{cx?= NUfvfmp1SW62aXXzeJJw\2WwaXOgZYN1cX[rdImgbY4hcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyCmcoXnJIxmfmWuIHPheZNqdmdiaX7obYJqfGmxbjDv[kAyODBicF2gSVIu\W6qYX7j[YQh[2WubDDwdo9tcW[ncnH0bY9vKHSxIHzleoVtKGWzdXn2ZYxmdnRidH:gOVAheE1iRUKgZYZ1\XJiOU[gbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDBZ5Rqfmm2eTC9JFAvOSEQvF2u NWXQcVl4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5OFM3OjBpPkG5PVQ{PjJyPD;hQi=>
T47D NYrp[29sSW62aXXzeJJw\2WwaXOgZZN{[Xl? M4rhUVk3KGi{cx?= NGm1bFFCdnSrZYP0do9o\W6rYzDhZ5Rqfmm2eTDpckBpfW2jbjDUOFdFKGOnbHzzJIF{e2W|c3XkJIF{KGS{dXegcIV3\WxiY3H1d4lv\yCrbnjpZol1cW:wIH;mJFExOCCyTTDFNk1mdmijbnPl[EBk\WyuIIDyc4xq\mW{YYTpc44hfG9ibHX2[Ywh\XG3aY\hcIVvfCC2bzC1NEBxVSCHMjDh[pRmeiB7NjDodpMh[nliYXzhcYFzKGKudXWgZZN{[XluIFHjeIl3cXS7IE2gNE4yKM7:TT6= NEXQZpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUm0N|YzOCd-MUm5OFM3OjB:L3G+
MCF7 MYPBcpRq\XO2cn;n[Y5q[yCjc4PhfS=> NGO1[lc6PiCqcoO= MV\BcpRq\XO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCPQ1[3JINmdGy|IHHzd4V{e2WmIHHzJIRzfWdibHX2[Ywh[2G3c3nu[{BqdmirYnn0bY9vKG:oIEGwNEBxVSCHMj3lcohidmOnZDDj[YxtKHC{b3zp[oVz[XSrb36geI8hdGW4ZXyg[ZF2cX[jbHXueEB1dyBzMDDwUUBGOiCjZoTldkA6PiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEGldHn2bZR6KD1iMD6yJO69VS5? MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl2M{[yNEc,OTl7NEO2NlA9N2F-
MCF7 NGDHdWdCdnSrZYP0do9o\W6rYzDhd5NigQ>? NFfTc5U6PiCqcoO= NWPLb3p3SW62aXXzeJJw\2WwaXOgZYN1cX[rdImgbY4hcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyCmcoXnJIxmfmWuIHPheZNqdmdiaX7obYJqfGmxbjDv[kAyODBicF2gSVIu\W6qYX7j[YQh[2WubDDwdo9tcW[ncnH0bY9vKHSxIHzleoVtKGWzdXn2ZYxmdnRidH:gNUBxVSCHMjDh[pRmeiB7NjDodpMh[nliYXzhcYFzKGKudXWgZZN{[XluIFHjeIl3cXS7IE2gNE41KM7:TT6= NH7QfGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUm0N|YzOCd-MUm5OFM3OjB:L3G+
T47D NEfWZVVCdnSrZYP0do9o\W6rYzDhd5NigQ>? NYPJWWZZQTZiaILz NGHs[mdCdnSrZYP0do9o\W6rYzDhZ5Rqfmm2eTDpckBpfW2jbjDUOFdFKGOnbHzzJIF{e2W|c3XkJIF{KGS{dXegcIV3\WxiY3H1d4lv\yCrbnjpZol1cW:wIH;mJFExOCCyTTDFNk1mdmijbnPl[EBk\WyuIIDyc4xq\mW{YYTpc44hfG9ibHX2[Ywh\XG3aY\hcIVvfCC2bzCxNEBxVSCHMjDh[pRmeiB7NjDodpMh[nliYXzhcYFzKGKudXWgZZN{[XluIFHjeIl3cXS7IE2gNE44KM7:TT6= Mlv6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7NEO2NlAoRjF7OUSzOlIxRC:jPh?=
T47D MVHBcpRq\XO2cn;n[Y5q[yCjc4PhfS=> NYfzN2x5QTZiaILz M3fjbGFvfGmnc4Tyc4dmdmmlIHHjeIl3cXS7IHnuJIh2dWGwIGS0O2Qh[2WubIOgZZN{\XO|ZXSgZZMh\HK3ZzDs[ZZmdCClYYXzbY5oKGmwaHnibZRqd25ib3[gNVAxKHCPIFWyMYVvcGGwY3XkJINmdGxicILvcIln\XKjdHnvckB1dyCuZY\lcEBmeXWrdnHs[Y51KHSxIEGgdG0hTTJiYX\0[ZIhQTZiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCDY4Tpeol1gSB;IEmg{txONg>? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl2M{[yNEc,OTl7NEO2NlA9N2F-
HepG2 Ml3HR5l1d3SxeHnjbZR6KGG|c3H5 NWTMV|BOOjRiaILz NUe4R|FsS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KHKnc3H6eZJqdiCmeXWtZoF{\WRiZnz1c5Jme2OnbnPlJIFv[Wy7c3nzMEBESzVyIE2gNlQvPyEQvF2u NHnmWVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEOyNlc2PCd-M{CzNlI4PTR:L3G+
HEK293 NFnmZpREgXSxdH;4bYNqfHliYYPzZZk> M4q0O|I1KGi{cx?= MofpR5l1d3SxeHnjbZR6KGGpYXnud5QhUEWNMkmzJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSC{ZYPhfpVzcW5iZInlMYJie2WmIH\seY9z\XOlZX7j[UBidmGueYPpd{whS0N3MDC9JFM6NjRizszNMi=> M4\4[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyM{KyO|U1Lz5|MEOyNlc2PDxxYU6=
MCF-7 MY\DfZRwfG:6aXPpeJkh[XO|YYm= MVzDfZRwfG:6aXPpeJkh[WejaX7zeEBOS0ZvNzDj[YxteyCrbjD0bIUh[WK|ZX7j[UBw\iBzIIXNJGUzKCinc4TyZYRqd2xrLDDDfZRwfG:6aXPpeJkhRSB7Lkig{txONg>? NYTIfphvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUC4PVM{Oid-OUC4PVM{OjxxYU6=
MCF-7 MUXDfZRwfG:6aXPpeJkh[XO|YYm= MWnDfZRwfG:6aXPpeJkh[WejaX7zeEBOS0ZvNzDj[YxteyCrbjDwdoV{\W6lZTDv[kAyKHWPIFWyJEhme3S{YXTpc4wqNCCFeYTveI95cWOrdImgQUAyOyEQvF2u NHvzWmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97MEi5N|MzLz57MEi5N|MzRC:jPh?=
MCF-7 MY\GeY5kfGmxbjDhd5NigQ>? NV25fnRkTW[oZXP0bZZmKGSxc3Wg[o9zKFt|SG2tJIV{fHKjZHnvcEBi\2GrboP0JJBzd2yrZnXyZZRqd25ib3[gUWNHNTdiY3XscJMtKEWFNUCgQUAxNjByMEGxJO69VS5? M3PTZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5Nkm2PFEoRjJ5Nkm2PFE9N2F-
Ishikawa MoTMSpVv[3Srb36gZZN{[Xl? MUTDc45k\W62cnH0bY9vKG:oIHPvcZBwfW6mIILldZVqemWmIITvJIlv\HWlZTC1NEAmKG:oIITo[UBu[XirbYXtJJN1cW23bHH0bY9vKG:oIHHsb4FtcW6nIIDoc5NxcGG2YYPlJIFkfGm4aYT5JIlvKEm|aHnrZZdiKGOnbHzzMEBGSzVyIE2gNE4xOzNizszNMi=> NUTOfIZvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOEKwNVU5Pyd-OEKwNVU5PzxxYU6=
MCF-7 NVHLeYI4TnWwY4Tpc44h[XO|YYm= NF2ybI1G\m[nY4TpeoUh[2:wY3XueJJifGmxbjDh[4FqdnO2IF3DSk04KGK{ZXHzeEB1fW2xcjDj[YxteyC3c3nu[{BOS0ZvNzDhd5NigS5uIFXDOVAhRSByLkCxJO69VS5? NFrpNlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkiyOVk{PSd-MUK4NlU6OzV:L3G+
ScN2a-cl3 M1[0cGZ2dmO2aX;uJIF{e2G7 NXrTfZhUPSCmYYnz NHrFVHFKdmirYnn0bY9vKG:oIGLNUEBxemmxbjDwdo91\WmwIHnu[oVkfGWmIHnuJI1wfXOnIHTpeollcW6pIGPjUlJiNWOuMzDj[YxteyCneIDy[ZN{cW6pIH\1cIwhdGWwZ4ToJI1wfXOnIGDyVEBie3Onc4Pl[EBieyC{ZXT1Z5Rqd25ib3[gVJJRe2NibHX2[Ywh[W[2ZYKgOUBl[Xm|IHL5JGVNUVODLDDFR|UxKD1iND6zPUDPxE1w MmnMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzOEO1PFkoRjJ2MUizOVg6RC:jPh?=
NCI-H460 NXTvbHZ1SW62aXPhcoNmeiCjc4PhfS=> NUfwOoFROjRiaILz NXjofYVbSW62aXPhcoNmeiCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJG5EUS2KNE[wJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCVUlKgZZN{[XluIFfJOVAhRSB2LkS4JO69VS5? NWnV[2VKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm3N|MxQDVpPkG5O|M{ODh3PD;hQi=>
DU145 M{\YTWFvfGmlYX7j[ZIh[XO|YYm= NG\icXMzPCCqcoO= NX;tNHZYSW62aXPhcoNmeiCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJGRWOTR3IHPlcIx{KGGodHXyJFI1KGi{czDifUBUWkJiYYPzZZktKEeLNUCgQUA3NjB5IN88UU4> Mk\QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl5M{OwPFUoRjF7N{OzNFg2RC:jPh?=
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MCF7 NI\wVYRCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MUK0PEBpenN? MXjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiZYjwdoV{e2mwZzDld5Rzd2enbjDy[YNmeHSxcjDpcoN2[mG2ZXSge4l1cCByLkCwN{BvVSCxZjDld5Rz[WSrb3ygZY5lKDBwMEGgcm0hd2ZidHXzeI9{fGW{b37lJIFnfGW{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJFUmKG[ndHHsJIJwfmmwZTDz[ZJ2dSxiR1m1NEA:KDJwMkmwPFch|ryPLh?= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO|U9N2F-
MCF7 NFTLc3FCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NFjwcGI1QCCqcoO= MnXERY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IHX4dJJme3Orbneg[ZN1em:pZX6gdoVk\XC2b4KgbY5kfWKjdHXkJJdqfGhiMD6wOkBvVSCxZjDld5Rz[WSrb3ygZY5lKDBwMEegcm0hd2ZidHXzeI9{fGW{b37lJIFnfGW{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJIZmfGGuIHLveolv\SC|ZYL1cUBidmRiToXT[ZJ2dSxiR1m1NEA:KDJwOUWxNlEh|ryPLh?= MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO|U9N2F-
MCF7 M4LQbmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MorSOFghcHK| NVm4do1JSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIV5eHKnc4Ppcoch\XO2cn;n[Y4hemWlZYD0c5IhcW6ldXLheIVlKHerdHigNVAxKG6PIH;mJIV{fHKjZHnvcEBidmRiMD6wO{BvVSCxZjD0[ZN1d3O2ZYLvcoUh[W[2ZYKgOFghcHK|IHL5JJN2dG[xcnjv[IFucW6nIFKgZZN{[XliaX6gdJJme2WwY3Wgc4Yh\mW2YXygZo93cW6nIIPldpVuKGGwZDDOeXNmenWvLDDHTVUxKD1iNT62NlM1OSEQvF2u MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO|U9N2F-
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MCF7 MULBcpRqfHWvb4KgZZN{[Xl? MnfEOlAhdWdxa3e= MVq0JJdm\Wu| M3XTdmFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgfIVvd2e{YX\0[YQhcW5iboWvcpUhdW:3c3WgcY9l\Wxib3[geIFud3irZnXuMZNmdnOrdHn2[UBjemWjc4SgZ4Fv[2W{IIjlco9oemGodDDpcZBt[W62ZXSge4l1cCBzN3LleIEu\XO2cnHkbY9tKHSrbXWgdoVt\WG|ZTDw[Yxt\XS|IHHzd4V{e2WmIHHzJJJm\HWldHnvckB1fW2xcjD2c4x2dWViYYSgOlAhdWdxa3esJJBwKHGmIH\vdkA1KHenZXvz NX2yXZpERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CwPFY3OjZpPkOwNFg3PjJ4PD;hQi=>
insect cells NEi5em9HfW6ldHnvckBie3OjeR?= MmD4NE4yKHSxIEGwNFAxKHWP NWHVR2lxSmmwZHnu[{Bi\m[rbnn0fUB1dyC3boTh[4dm\CCqdX3hckBz\WOxbXLpcoFvfCCodXzsJIxmdme2aDDFVoFteGijIHX4dJJme3OnZDDpckBqdnOnY4SgZ4VtdHNiMD6xJJRwKDFyMECwJJVOKGK7IF\seY9zdW:wZTDFV|IhT3KnZX6g[Il{eGyjY3Xt[Y51KGKjc3XkJIF{e2G7 M{\xXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMEmxPVIxLz5|MEC5NVkzODxxYU6=
アッセイ
Methods Test Index PMID
Western blot p-AKT / AKT ; ER-α66 / ER-α36 / HER2 / EGFR ; p-AMPK / AMPK / p-ACC / ACC / p-P70S6K / p-S6 / p-4EBP1 ; CD36 / p-ERK / ERK 30759864 25203051 27020861 30573731
Immunofluorescence CD36 30573731
体内試験

Tamoxifen (TAM) is widely used for both treatment and prevention of breast cancer. However, it is also carcinogenic in human uterus and rat liver[2]. Tm-inducible Cre-loxP systems are being used in broad areas of research and are providing important biologic insights in tissue development, maintenance, and function. Tamoxifen-induced nuclear localization of Cre recombinase is time- and dose-dependent. Higher doses of tamoxifen induce recombination weeks following administration and Lower doses of tamoxifen induce recombination up to one week following administration. Duration of tamoxifen-induced gene recombination is also dose-dependent. Administration of high Tm doses leads to extended CreER nuclear localization. Tm treatment induces side effects that may have physiologic consequences in Tm-inducible models[4].

お薦めの試験操作(参考用のみ)

細胞試験:

[2]

  • 細胞株:MCF7 and T47D
  • 濃度: 10-6 mol/L
  • 反応時間:24, 48, and 96 h
  • 実験の流れ:

    48 h before the addition of E2 and TAM, cells were washed with 5 mL phosphate-buffered saline (PBS) and then switched to phenol red-free RPMI-1640 containing 10% charcoal-stripped FBS. E2 and TAM were dissolved in absolute ethanol and diluted in the media at 10-8 mol/L and 10-6 mol/L, respectively, and then added to the culture medium at 24, 48, and 96 h.

動物試験:

[4]

  • 動物モデル:Pdx1PB-CreERTm mice
  • 投薬量:--
  • 投与方法:s.c.

溶解度 (25°C)

体外

体内

左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
混合させたのち直ちに使用することを推奨します。

1mg/mL

化学情報

分子量 371.51
化学式

C26H29NO

CAS No. 10540-29-1
Storage 3年 -20°C
2年 -80°C in solvent
Smiles CCC(=C(C1=CC=CC=C1)C2=CC=C(C=C2)OCCN(C)C)C3=CC=CC=C3

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

便利ツール

質量 濃度 体積 分子量

臨床試験

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04200066 Not yet recruiting Drug: Temozolomide Tamoxifen Maprotiline Glioblastoma|Brain Tumor University of Rochester June 1 2022 Phase 1
NCT05133674 Active not recruiting Drug: Tamoxifen 20 mg Breast Cancer|Breast Carcinoma|Breast Tumors|Cancer of Breast|Malignant Neoplasm of Breast Karolinska University Hospital April 4 2022 Phase 2
NCT05156892 Not yet recruiting Drug: SUBA-itraconazole|Drug: Tamoxifen Ovarian Cancer Anthony Joshua FRACP|Royal Prince Alfred Hospital Sydney Australia|Concord Hospital|Prince of Wales Hospital Sydney|St Vincent''s Hospital Sydney February 1 2022 Phase 1
NCT05378269 Recruiting Drug: Tamoxifen|Other: Placebo Vulvar Atrophy Daré Bioscience Inc. November 22 2021 Phase 1|Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

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お問い合わせ内容をご入力ください

よくある質問(FAQ)

質問1:
Could you tell me if you have a protocol for the use of tamoxifen (S1238) in vivo in mouse? Is there an administration way that is better than another?

回答
Tamoxifen (S1238) can be dissolved in 10% ethanol/10% Tween 80/ddH2O at 5 mg/ml clearly. But precipitation will form if the formulation was left at RT for an hour or longer. So we'd suggest that you use it quickly after formulation. Tamoxifen is often administrated via i.p injection.

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