PD98059

製品コード:S1177

For research use only.

PD98059 is a non-ATP competitive MEK inhibitor with IC50 of 2 μM in a cell-free assay, specifically inhibits MEK-1-mediated activation of MAPK; does not directly inhibit ERK1 or ERK2. PD98059 is a ligand for the aryl hydrocarbon receptor (AHR) and functions as an AHR antagonist.

PD98059化学構造

CAS No. 167869-21-8

サイズ 価格(税別)
10mM (1mL in DMSO) JPY 24200
JPY 26400
JPY 80000
JPY 113200

代表番号: 03-5615-9297|電子メール:[email protected]
よく尋ねられる質問

文献中Selleckの製品使用例(435)

製品安全説明書

MEK阻害剤の選択性比較

相関MEK製品

生物活性

製品説明 PD98059 is a non-ATP competitive MEK inhibitor with IC50 of 2 μM in a cell-free assay, specifically inhibits MEK-1-mediated activation of MAPK; does not directly inhibit ERK1 or ERK2. PD98059 is a ligand for the aryl hydrocarbon receptor (AHR) and functions as an AHR antagonist.
特性 Does not inhibit c-Raf phosphorylated MEK1.
ターゲット
AhR [1]
(Cell-free assay)
MEK1 [1]
(Cell-free assay)
1 μM 2 μM
体外試験

PD98059 inhibits either basal MEK1 or a partially activated MEK produced by mutation of serine at residues 218 and 222 to glutamate (MEK-2E) with IC50 of 2 μM. PD98059 does not inhibit the MAPK homologues JNK and P38. PD98059 is highly selective against MEK, as it does not inhibit a number of other kinase activities including Raf kinase, cAMP-dependent kinase, protein kinase C, v-Src, epidermal growth factor (EGF) receptor kinase, insulin receptor kinase, PDGF receptor kinase, and phosphatidylinositol 3-kinase. PD98059 inhibits PDGF-stimulated activation of MAPK and thymidine incorporation into 3T3 cells with IC50 of ~10 μM and ~7 μM, respectively. [1] PD98059 potently prevents the activation of MEK1 by Raf or MEK kinase with IC50 of 4 μM, and weakly inhibits the activation of MEK2 by Raf with IC50 of 50 μM. PD98059 does not inhibit the activation of MEK homologues MKK4 and RK kinase that participate in stress and interleukin-1-stimulated kinase cascades in KB and PC12 cells, and the activation of p70 S6 kinase by insulin or epidermal growth factor in Swiss 3T3 cells. [2] PD98059 completely blocks the nerve growth factor (NGF)-induced differentiation of PC12 cells without altering cell viability. [3] PD98059 inhibits the proliferation of RAW264.7 cells in the culture containing RANKL in a dose-dependent manner, resulting in an apparent decrease of TRAP-positive cells. [4]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A2780  MXPGeY5kfGmxbjDBd5NigQ>? NGjIbFMzOMLizszN MVixJIg> Mn7VZoxw[2u|IFTUR2QucW6mdXPl[EBFWjViZYjwdoV{e2mxbh?= M2TLcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|Nkm2PFYzLz5{M{[5Olg3OjxxYU6=
AML 1# MX;BdI9xfG:|aYOgRZN{[Xl? Moi3NlDDqM7:TR?= MVyxNkBp NU\3ZZF5\W6qYX7j[ZMh[2WubDDhdI9xfG:|aYOgbY5lfWOnZDDifUBUOQ>? M4\xXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{C2OlkyLz5{M{ewOlY6OTxxYU6=
KG-1  MoLlRZBweHSxc3nzJGF{e2G7 MX6yNOKh|ryP MXGxNkBp MmPV[Y5p[W6lZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDTNS=> NV62U|E3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFY3QTFpPkKzO|A3PjlzPD;hQi=>
HUVECs Mn21RZBweHSxc3nzJGF{e2G7 NEDXXHAzNzRizszN M3zsO|I1NzR6IHi= NWHwPYdocW6mdXPld{Bk\WyuIHTlZZRp Mmq4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5MEe1NlAoRjJ|N{C3OVIxRC:jPh?=
HepG2  MXPGeY5kfGmxbjDBd5NigQ>? NV3NXJBbOjEEoN88US=> Mm\kNlQhcA>? M17GSYlvcGmkaYTzJJRp\SCKTz2xJJBzd3SnaX6g[ZhxemW|c3nvckBkdy22cnXheIVlKHerdHigcYV1\m:{bXnu NWjueZFoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFc3ODlpPkKzO|A4PjB7PD;hQi=>
NB4  NY\ke5gySXCxcITvd4l{KEG|c3H5 NV\WcnpQOTBxMkCvOlAh|ryP MU[xMlUhcA>? NYjFfZduTE2VTx?= NIj5WY9l\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgZ48ufHKnYYTl[EB4cXSqIGDhZ4xqfGG6ZXy= NEH6PJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ezOVU1OSd-MkO3N|U2PDF:L3G+
BxPC-3 cells NVrkNmN2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXjvNnZFOjEEoN88US=> MYiwMlUhcA>? NXTvc|FjcW6qaXLpeJMhXkWJRj3BMZJm\3WuYYTl[EBJXV[HQzDndo94fGhiYX7kJJR2[mViZn;ycYF1cW:wIHnu[JVk\WRiYomgVGFTNTJiQWC= NVfJ[nVDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3OlQxPDZpPkKzO|Y1ODR4PD;hQi=>
MKN45 MnnyRZBweHSxc3nzJGF{e2G7 M4nadVExKM7:TdMg MlLaNlQhcA>? NV\aSpFXcW6lcnXhd4V{KHSqZTDERXBVNWmwZIXj[YQh[2WubDDhdI9xfG:|aYO= NVTI[nlxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PVI2QDhpPkKzO|kzPTh6PD;hQi=>
SGC7901 NYCxVJBoSXCxcITvd4l{KEG|c3H5 M1G1Z|ExKM7:TdMg MV:yOEBp M{T3UYlv[3KnYYPld{B1cGViRFHQWE1qdmS3Y3XkJINmdGxiYYDvdJRwe2m| MlTDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUK1PFgoRjJ|N{myOVg5RC:jPh?=
MKN45 M3PZWGZ2dmO2aX;uJGF{e2G7 M1vCeFExKM7:TdMg NGDMWHAzPCCq MUfpcohq[mm2czD0bIUh\XiycnXzd4lwdiCxZjDwbI9{eGixconsZZRm\CCHUluxM|I> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd7MkW4PEc,OjN5OUK1PFg9N2F-
SGC7901 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4S0U|ExKM7:TdMg MV2yOE81QC95MjDo MkDLbY5pcWKrdIOgZ4VtdCCpcn;3eIgh[29vdILlZZRm\CC5aYToJGRCWFR? MnvyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUK1PFgoRjJ|N{myOVg5RC:jPh?=
MKN45 NH3BWYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjmcmg3OTBizszNxsA> M3v2T|I1NzR6L{eyJIg> MYLpcohq[mm2czDj[YxtKGe{b4f0bEBkdy22cnXheIVlKHerdHigSGFRXA>? NWmwSG92RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PVI2QDhpPkKzO|kzPTh6PD;hQi=>
SGC7901 M3fvTGZ2dmO2aX;uJGF{e2G7 NWDxb4xqOTBizszNxsA> M4PIZ|I1KGh? NX\SOpZGcW6qaXLpeJMhfGinIHX4dJJme3Orb36gc4YheGixc4Doc5J6dGG2ZXSgSXJMOS9{ NGP3SWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e5NlU5QCd-MkO3PVI2QDh:L3G+
HUASMCs MlvwSpVv[3Srb36gRZN{[Xl? NFzJNHUyOCEQvF5CpC=> NEXXdpkzPCCq MYPkbY1qdmm|aHXzJGFv\yCLST3jZZV{\WRiU1;DV|MhdVKQQTDhcoQheHKxdHXpckBmgHC{ZYPzbY9vyqB? NVfXRoxNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4NVY1PjhpPkKzPFE3PDZ6PD;hQi=>
HUASMCs MlPDSpVv[3Srb36gRZN{[Xl? MUOxNEDPxE4EoB?= M4XCUlI1KGh? MVzpcohq[mm2czDBcochUUlvaX7keYNm\CCHUluxM|IheGixc4Doc5J6dGG2aX;uJIxmfmWu NVXZXplZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4NVY1PjhpPkKzPFE3PDZ6PD;hQi=>
EPOR/CR3 NXHiU406TnWwY4Tpc44hSXO|YYm= NEHuVlk2OMLizszN NIPrZXI{KGh? NHvrfZpz\WS3Y3XzJGVRVyCjbnSvc5IhUUxvMz3pcoR2[2WmIITo[UB1gXKxc3nu[UBxcG:|cHjvdplt[XSrb39CpC=> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{MEezNUc,OjN6MkC3N|E9N2F-
NB4  M2Prd2Z2dmO2aX;uJGF{e2G7 NIf6ZYUyOCEQvF5CpC=> MoXpO|IhcA>? MWfEUXNQ NVTBXo5ZcW6qaXLpeJMh\GG|YYTpcoljNWmwZIXj[YQhS0RzMXKg[ZhxemW|c3nvci=> M3r6[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEK1OVg2Lz5{M{iyOVU5PTxxYU6=
HL60  MmW0SpVv[3Srb36gRZN{[Xl? MUiyNOKh|ryP NGnTNHc4OiCq NWTLeVE{TE2VTx?= M3nheIlvcGmkaYTzJIRie2G2aX7pZk1qdmS3Y3XkJGNFOTGkIHX4dJJme3Orb36= MonyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MkW1PFUoRjJ|OEK1OVg2RC:jPh?=
HUVECs NEnIWoNHfW6ldHnvckBCe3OjeR?= MoXoNlUh|ryP Mo\uNUBp M4nnPIlv[3KnYYPlJG5HNc78QjDwOlUhdnWlbHXhdkB1emGwc3zvZ4F1cW:w NF\Sclg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mwNVAxQCd-MkO5NFExODh:L3G+
LNCaP  NE\2dYtHfW6ldHnvckBCe3OjeR?= MXmxNEDPxE4EoB?= MX6xJIg> M{HCdoRm[3KnYYPld{B1cGViRVfGJJVxemWpdXzheIVlKHBvWVKtNS=> MkS0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6M{izNVgoRjJ|OEO4N|E5RC:jPh?=
HL-60 NY\zS|lCSXCxcITvd4l{KEG|c3H5 MoGzOVDDqM7:TR?= NVrrdZZOOSCq NVf2fFk6emW|Y4Xld{BDSTF2NTDt[YRq[XSnZDDhdI9xfG:|aYO= NUD2O2IzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5OFg4PTFpPkKzPVQ5PzVzPD;hQi=>
HepG2 M2rD[mZ2dmO2aX;uJGF{e2G7 M4fxdVQxKM7:TR?= MYS2M|EzKGh? M2\BT4lvcGmkaYTzJJRp\SCrbnPy[YF{\SCxZjDwMWVTUzFiYX7kJJAu[y2MdX6gdJJwfGWrbjDlfJBz\XO|aX;uJIJ6KFCO NGnlcFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m0Nlg2OSd-MkO5OFI5PTF:L3G+
HPAF-II M1\2PGZ2dmO2aX;uJGF{e2G7 MVqxNEDPxE4EoB?= Mn;vOkBp MoXxbY5kemWjc3WgcYlTNTF2MzDlfJBz\XO|aX;u Ml35QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7N{O3NVAoRjJ|OUezO|ExRC:jPh?=
BxPC-3 NV;xcFV{TnWwY4Tpc44hSXO|YYm= NXXmVHI6OTBizszNxsA> M4i5PVYhcA>? MWnpcoNz\WG|ZTDtbXIuOTR|IHX4dJJme3Orb36= M3iyb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUezO|ExLz5{M{m3N|cyODxxYU6=
COLO205 MWnBdI9xfG:|aYOgRZN{[Xl? M{fjO|ExNzJyL{SwJO69VQ>? Mn\qNlQhcA>? Ml;PbY5lfWOnczDEUmEhdGGmZHXyJIZwem2jdHnvci=> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDBzOUGwPEc,OjRyMUmxNFg9N2F-
G292  M3nobmFxd3C2b4Ppd{BCe3OjeR?= MXOzNOKh|ryP NF[zZVEzKGh? MkPRdoV{fG:{ZYOgZ4Fxe2GrY3nuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> MoW3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUK5N|AoRjJ2MEGyPVMxRC:jPh?=
SGC7901 MX\BdI9xfG:|aYOgRZN{[Xl? MUOyNOKh|ryP MX:xJIg> M3\mXolvcGmkaYTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEmITj5OteKh[W6mIEZihNIuTE[XUh?= NHOxRmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECyO|c2OCd-MkSwNlc4PTB:L3G+
MGC803  Mn7XRZBweHSxc3nzJGF{e2G7 MXSyNOKh|ryP NXn1UJl3OSCq M3;RV4lvcGmkaYTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEmITj5OteKh[W6mIEZihNIuTE[XUh?= M4HSWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEK3O|UxLz5{NECyO|c2ODxxYU6=
HPMC NWGwOXJOSXCxcITvd4l{KEG|c3H5 MmHjNVDDqM7:TR?= NH;TWJczPMLiaB?= Mn7TdoV3\XK|ZYOg[IVkemWjc3WgbY4h[2WubDD2bYFjcWyrdImgbY5lfWOnZDDifUBJT1CGUx?= M1;BUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MESyPFM5Lz5{NEC0Nlg{QDxxYU6=
HCT-8 NFvV[|hCeG:ydH;zbZMhSXO|YYm= MnjXNVDDqM7:TR?= NYn3dpZpOjUEoHi= NXG1VW14\GWlcnXhd4V{KGOnbHygZZBweHSxc3nzJIlv\HWlZXSgZpkhPS2IVR?= MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7NUi2N{c,OjRyOUW4OlM9N2F-
HPMC NIPr[lNHfW6ldHnvckBCe3OjeR?= MVOxNOKh|ryP NWDSOFdPPDhiaB?= MknCdoV3\XK|ZYOgeIhmKGOqYX7n[ZMhcW5iY3XscEBud3KyaH;sc4d6KGmwZIXj[YQh[nliSFfQSHM> NYHCeIxqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwOFI5OzhpPkK0NFQzQDN6PD;hQi=>
A549 M{myd2Z2dmO2aX;uJGF{e2G7 MV:1NOKh|ryP NWnFV2dtOiCq MXLicI9kc3NiRWLLJJBpd3OyaH;yfYxifGmxbjDt[YRq[XSnZDDifUAyNDJvTmG= NIrDbWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC2O|czPyd-MkSwOlc4Ojd:L3G+
Caco-2  NVTDV2J3SXCxcITvd4l{KEG|c3H5 M2jSTFExyqEQvF2= NGHNVGIzPMLiaB?= M2L6NYRm[3KnYYPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JFUuTlV? MnfyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUW4OlMoRjJ2MEm1PFY{RC:jPh?=
AGS MV7GeY5kfGmxbjDBd5NigQ>? MX:xNEDPxE4EoB?= MUmwMlUhcA>? M2C3ZYlvcGmkaYTzJJRp\SC3cILl[5Vt[XSrb36gc4YhfGinIFnMMVgh\2WwZR?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFyNkG2Okc,OjRzME[xOlY9N2F-
Ca9-22 M1LSTGZ2dmO2aX;uJGF{e2G7 NEfHVGE{KM7:TR?= NIqzcpgyNzJiaB?= MnrWdoVlfWOnczDIZnIucW6mdXPl[EBCXEZvMjDwbI9{eGixconsZZRqd25? M370WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUK2OVMzLz5{NEGyOlU{OjxxYU6=
Ca9-22 MYrGeY5kfGmxbjDBd5NigQ>? M2TS[FMh|ryP NEDPNGEyKGh? Mnn0ZYJwdGm|aHXzJJRp\SCjYnnsbZR6KG:oIFjiVkB1dyCrbnT1Z4UhUUxvODDwdo9lfWO2aX;u M3\4[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUK2OVMzLz5{NEGyOlU{OjxxYU6=
HAECs NHH0UHlHfW6ldHnvckBCe3OjeR?= MluyNVAh|ryP M3Tm[|EhcA>? NYLYVFBnyqCjdITlcpVifGW|IGTOSk3PuS2|dHnteYxifGWmIFnDRW0uOSCjbnSgWmNCVS1zIHX4dJJme3Orb36= MmLYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzM{S2OVcoRjJ2MUO0OlU4RC:jPh?=
Caco-2 NInqToxHfW6ldHnvckBCe3OjeR?= NFH0RnI2OMLizszN NUn3SXgzPDiq NYCwVGtjTE2VTx?= MoDY[Y5p[W6lZYOgeIhmKG2UTlGgcIV3\Wy|IH;mxsBUS06QMVGsSnh[TDNuwrDMR3QtyqCOT2isxsBJUUZ|QT|CpHpIOTZuwrDQSGU3ScLiYX7kxsBNT0GOU{G2xsBo\W6nc9MgZ48ufHKnYYTl[EB4cXSqIFTlfC=> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF4MU[5OUc,OjRzNkG2PVU9N2F-
MCF-7 M2LTVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX2xNEDPxE1? MUC0PIg> NUPKbW1JTE2VTx?= MkTsdoV3\XK|ZYOgRm5HNWmwZIXj[YQh[2WubDDjfYNt\SCjcoLld5Q> NGntfpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG2N|QxPCd-MkSxOlM1ODR:L3G+
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DLD-1  NFrqZYJHfW6ldHnvckBCe3OjeR?= MViyNOKh|ryP NH\LXI01QCCq Ml;HdoVlfWOnczD0bIUhSk6LUEOg[ZhxemW|c3nvckBxemVvdILlZZRm\CC5aYToJFUu[XqjLXTD MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJzMUW4NUc,OjR{MUG1PFE9N2F-
HT-29 NFvH[WRHfW6ldHnvckBCe3OjeR?= NHXWN28zOMLizszN M2HUWVQ5KGh? NFnZdHVz\WS3Y3XzJJRp\SCETlnQN{BmgHC{ZYPzbY9vKHC{ZT30doVifGWmIIfpeIghPS2jenGt[GM> NITNdFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKxNVU5OSd-MkSyNVE2QDF:L3G+
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ARPE-19 NXOxXYpJTnWwY4Tpc44hSXO|YYm= M1rkcFIxKM7:TR?= M1\xfFAvPSCq MX7pcohq[mm2czDBdIVtcW5vaX7keYNm\CCyaH;zdIhwenmuYYTpc44hd2ZiRYLrJIFv\CCDa4S= NYS0UnZmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNlc6OThpPkK0NlI4QTF6PD;hQi=>
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KATOIII  NF7ue4JHfW6ldHnvckBCe3OjeR?= NW\DU|Q5PTBizszN M3\uO|Q5KGh? NFiydXpFVVOR MkPLbY5kemWjc3XzJIV5eHKnc4Ppc44hd2ZiSFzBMWExOiCxcjDIUGEuSTJ2IH3vcIVkfWyncx?= Mn[4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{NESwNlMoRjJ2MkS0NFI{RC:jPh?=
NCI-N87  NEPTUXZHfW6ldHnvckBCe3OjeR?= NWfSPYZyPTBizszN NH:xOWM1QCCq NWiyRmd6TE2VTx?= M4jYbolv[3KnYYPld{BmgHC{ZYPzbY9vKG:oIFjMRU1CODJib4KgTGxCNUF{NDDtc4xm[3WuZYO= NY\XfG15RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
OE19 M3y2VGZ2dmO2aX;uJGF{e2G7 NV;5OppoPTBizszN M2KwXVQ5KGh? MXPEUXNQ M4OyVIlv[3KnYYPld{BmgHC{ZYPzbY9vKG:oIFjMRU1CODJib4KgTGxCNUF{NDDtc4xm[3WuZYO= NULoZ49GRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
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HT29 MWrGeY5kfGmxbjDBd5NigQ>? NILRNHYyOCEQvF2= MnXQNkBp MW\pcohq[mm2czDv[kBLSUt{LDDFVmsyNzJiYX7kJHNVSVR|IIDoc5NxcG:{eXzheIlwdg>? M{XXeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mk[1Nlk{Lz5{NEK2OVI6OzxxYU6=
HepG2 MmTmRZBweHSxc3nzJGF{e2G7 Mk[3NlAh|ryP NYPWO4xbOjRiaB?= MkG0bY5pcWKrdIOgSXJMOS9{IIDoc5NxcG:{eXzheIlwdiCjbnSg[Y5p[W6lZYOgWmIyNWmwZIXj[YQh[XCxcITvd4l{ MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ2N{mwPUc,OjR{NEe5NFk9N2F-
HepG2 NIG0eW5HfW6ldHnvckBCe3OjeR?= NEjr[VIzOCEQvF2= NETNZ3czKGh? NGLTc4VmdmijbnPld{BXSjFvaX7keYNm\CCIT2jPN4EhfHKjboPjdolxfGmxbnHsJIFkfGm4aYT5 NID3cIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK0O|kxQSd-MkSyOFc6ODl:L3G+
MC-3 M2\seWFxd3C2b4Ppd{BCe3OjeR?= NFfwR2EyOCEQvF2= MVOyOEBp MmPldI91\W62aXH0[YQhVUWVQz3pcoR2[2WmIHHwc5B1d3OrczDpckAh[2WubIO= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ5MEWyN{c,OjR{N{C1NlM9N2F-
Raji  NWPMfpNkTnWwY4Tpc44hSXO|YYm= MXKxNEDPxE1? NX7qbGMzOSCq M1yzR4Jtd2OtczDod2JCTkZiaX7keYNm\CCHcnuxM|IheGixc4Doc5J6dGG2aX;u NFyzN3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK2PVY{OCd-MkSyOlk3OzB:L3G+
Raji  MnyyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWWxNEDPxE1? MXexJIg> MYXpcohq[mm2czD0bIUh[mG|YXygc5IhcHOEQV\GMZN1cW23bHH0[YQh[2WubDDwdo9tcW[ncnH0bY9vKGGwZDD2bYFjcWyrdIm= NVTJSItRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOlk3OzBpPkK0NlY6PjNyPD;hQi=>
A549 NGmyO2ZHfW6ldHnvckBCe3OjeR?= M37UU|MxKM7:TR?= NXXCe3puOC53IHi= MV3EUXNQ MX;pcohq[mm2czD0bJJwdWKrbj3pcoR2[2WmIFOvSWJR|rJiVHjyNlM2yqCyaH;zdIhwenmuYYTpc44> MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ5N{[5Okc,OjR{N{e2PVY9N2F-
HCSMCs MlHrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFv0TmsyOCEQvF2= NV;rVVdROjRiaB?= NWPvUXlM[myxY3vzJGZCSlB2LXnu[JVk\WRiSFPBV21EKHC{b3zp[oVz[XSrb36= M1zUe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{GyN|gyLz5{NEOxNlM5OTxxYU6=
PANC-1 M3PRUWZ2dmO2aX;uJGF{e2G7 M1XzV|IxKM7:TR?= MmDGOFghcA>? M3;GVolvcGmkaYTzJJRp\SCneIDy[ZN{cW:wIH;mJO6VPkRiaX6gdoV{eG:wc3WgeI8hfGinIGDQRXLPvMLiYXfvcol{fMLi NHXvN3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK5OFE{Oyd-MkSyPVQyOzN:L3G+
A549 M1PlSmZ2dmO2aX;uJGF{e2G7 NGPiSGY{OCEQvF2= Ml;hNE42KGh? M{K3dWROW09? MYHpcohq[mm2czD0bIUhfGi{b33ibY4ucW6mdXPl[EBKVC16L1PYR2w5NUy3YzDhZ5Rqfmm2eR?= NH\xbHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK3O|Y6Pid-MkSyO|c3QTZ:L3G+
SW480 M3XFZ2Z2dmO2aX;uJGF{e2G7 NWjnWnRlOTBizszN MnzDNlAhcA>? NG\YZXFFVVOR M{\WTJN2eHC{ZYPz[ZMhfGinIFPSWEBi[3Srdnn0fS=> M{HPVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{K0N|Y3Lz5{NEOyOFM3PjxxYU6=
HEK 293 NVSyfW5vTnWwY4Tpc44hSXO|YYm= NUHib|VnOTBizszN NIjHNIg2KGh? MVjEUXNQ MYDpcohq[mm2czDXcpQucW6mdXPl[EDPui2lYYTlcolvN1SFRkSgZYN1cX[rdImgZY5lKG63Y3zlZZIh|rJvY3H0[Y5qdiCjY3P1cZVt[XSrb36= M4PuXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{K0N|Y3Lz5{NEOyOFM3PjxxYU6=
HEK 293 MoLaSpVv[3Srb36gRZN{[Xl? NXzCSndmOTBizszN MXG1JIg> MXnEUXNQ NEfCNnV{fXCycnXzd4V{KHSqZTDDVnQh[WO2aY\peJk> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN{NEO2Okc,OjR|MkSzOlY9N2F-
HL-60  NF;NZnpHfW6ldHnvckBCe3OjeR?= NVntb5ZlOTBxMkCg{txO MYOxJIg> MoHKbY5pcWKrdIOgeIhmyqCQLjDjbIlv\W6|aYPlfJRz[WO2wrDpcoR2[2WmIHTp[oZmemWwdHnheIlwdiCrboTvJIdz[W63bH;jfZRmew>? MlW3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NUewNlAoRjJ2M{W3NFIxRC:jPh?=
HL-60 M3nqb2Z2dmO2aX;uJGF{e2G7 MlX2NkDDvU1? MoC2NVYhcA>? NUPBO|I1TE2VTx?= MVvpcohq[mm2czD0bIUh[XO|b3PpZZRqd25ib3[gdHM3OjFiUnHmMVEh[W6mIF7GRXRkOyxiYX7kJJRp\SCUQT3pcoR2[2WmIIDoc5NxcG:{eXzheIlwdiCxZjDueYNt\WG{IF7GRXRkOw>? NE\ycWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOzNFA3QCd-MkSzN|AxPjh:L3G+
HeLa MUnGeY5kfGmxbjDBd5NigQ>? MW[1NEDPxE1? MViwMlUhcA>? NYDoe3hy[myxY3vzJHRTYC1zIH71Z4xm[XJibXnndoF1cW:wIHHu[EBVYE6LUDDkc5dvNXKnZ4XsZZRqd25? MoiyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|N{[4NlcoRjJ2M{e2PFI4RC:jPh?=
HUVECs NVftSGRNTnWwY4Tpc44hSXO|YYm= NEPkc|YyOMLizszN MnO1NUBp NX3WXYp4cW6qaXLpeJMhfGinIFjEUEBz\WS3Y3XkJGNQYC1{IHX4dJJme3Orb36gZY5lKFCJST2yJJJmdGWjc3W= NEnRUno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO4OVExQSd-MkSzPFUyODl:L3G+
MCF-7 NFO0[IdHfW6ldHnvckBCe3OjeR?= NVnPXnRLOTEEoN88US=> NU\Ie3o{OTBxM{CgcYlv NYXaOZFPemWmdXPld{B1cGViVWTQMYRmeGWwZHXueEBGWkticHjvd5Bpd3K7bHH0bY9v NUTIUVY6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzPVA5OTlpPkK0N|kxQDF7PD;hQi=>
HGC-27 NUTic2p[SXCxcITvd4l{KEG|c3H5 NX\YVW96OSEEtV2= M1\DeFEhcA>? MV3zeZBxemW|c3XzJHJCTDByMTDwcJV{KE2NLUKyNFYucW6mdXPl[EBk\WyuII\pZYJqdGm2eTDsc5N{ NIDWTXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NESxOlM1QSd-MkS0NVY{PDl:L3G+
PC3  MmfVRZBweHSxc3nzJGF{e2G7 M4nIcVUxKM7:TR?= NFPlZ5MxNjViaB?= MWXpcohq[mm2czDNTHkuPDR7LXnu[JVk\WRiYYDvdJRwe2m|wrC= M1[4dlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEK0PFg6Lz5{NESyOFg5QTxxYU6=
HPAEpiCs  MXXGeY5kfGmxbjDBd5NigQ>? MV[zNEDPxE1? M1HzVlEhcA>? MUXpcohq[mm2czDUUmYu|rFic4TpcZVt[XSnZDDwOFIweDR2IF3BVGsheGixc4Doc5J6dGG2aX;u NVjETXpjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OFE5PzBpPkK0OFQyQDdyPD;hQi=>
BeWo MVLGeY5kfGmxbjDBd5NigQ>? NYTON5B[OTEEoN88US=> NEKzelQzKGh? M4DlbYlvcGmkaYTzJGVTUzFxMh?= NUD3bVlZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0N|M5PDZpPkK0OFM{QDR4PD;hQi=>
A498 M{\w[GFxd3C2b4Ppd{BCe3OjeR?= MnHBOVDDqM7:TR?= MkX2NlQhcA>? NWfXT4g6eG:2ZX70bYF1\XQEoITo[UBxem9vYYDvdJRwfGmlIHXm[oVkfHNib3[gUmM> MmLPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MEi0O|YoRjJ2NUC4OFc3RC:jPh?=
NHBE NY\WR5hHTnWwY4Tpc44hSXO|YYm= M3;mOVIwOjBizszN Mn;ENkBp MV;heJRmdnWjdHXzJGlNNTN|IIP0bY12dGG2ZXSgR3hEVDhxSVytPEB{\WO{ZYTpc44> NHjwPGE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES3PVUzPid-MkS0O|k2OjZ:L3G+
A375 NHH4XphE\WyuIFnueoF{cW:wIFHzd4F6 MnLQNVDjiJN{MDFCuW0> MYeyOEBp MofYdoVlfWOnczDt[Yxidm:vYTDj[YxtKGmwdnHzbY9v NFTwPVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES2OlA{Pid-MkS0OlYxOzZ:L3G+
HBMEC NWW3b5MyTnWwY4Tpc44hSXO|YYm= MWmxNEDPxE1? MVWxJIg> M2nxfIJtd2OtczDWSWdHNWmwZIXj[YQhTXCqQUKg[ZhxemW|c3nvci=> NWTzXGxORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OVg6QDJpPkK0OFU5QTh{PD;hQi=>
HCT-15 NHXtR3pHfW6ldHnvckBCe3OjeR?= MXGxJIg> NWnqVXdi[XS2ZX71ZZRmeyCSR1WyMYlv\HWlZXSgdIhwe3Cqb4L5cIF1cW:wIH;mJGVzc8Li MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR|MUSyOUc,OjV2M{G0NlU9N2F-
HCT-15 MW\BdI9xfG:|aYOgRZN{[Xl? M4PzclEhcA>? MlTVZYJwdGm|aHXzJJRp\SCycn;0[YN1cX[nIHXm[oVkfHNib3[gVGdGOsLiYXfhbY5{fCCldYLjeY1qdi2rbnT1Z4VlKGGyb4D0c5Nqew>? MorMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{G0NlUoRjJ3NEOxOFI2RC:jPh?=
786-O NH;FXVVCeG:ydH;zbZMhSXO|YYm= Mmi3OVDDqM7:TR?= NV20dHFiOjRiaB?= MV;wc5RmdnSrYYTld:KhfGinIIDyc{1ieG:ydH;0bYMh\W[oZXP0d{Bw\iCQQx?= NGP1XoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWwPFQ4Pid-MkS1NFg1PzZ:L3G+
HepG2  NXfKW3UyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3HEelIxKM7:TR?= NXrxTYk1OjRiaB?= NUTXfZhTe3WycILld5NmeyCWR1[t{tIyNWmwZIXj[YQh[2WubDDwdo9tcW[ncnH0bY9vKGGwZDDpcpZie2mxbh?= M1\TZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NU[wOFg5Lz5{NUW2NFQ5QDxxYU6=
SW480 MXHGeY5kfGmxbjDBd5NigQ>? M2PyVFIxyqEQvF2= M2fMPVHDqGh? NVPiN3hsemWmdXPld{B1cGViZYjwdoV{e2mxbjDv[kBCXEZ|IIDyc5RmcW5? MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR2N{ixOkc,OjV2NEe4NVY9N2F-
MDA-MB-231 MXjGeY5kfGmxbjDBd5NigQ>? M2HFbFI2KM7:TR?= NWmyPXBbOi1|IHi= MmjO[IVkemWjc3XzJJAuTVKNMT:yJIFv\CCVMUCwRVQh\XiycnXzd4lwdg>? MnHNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NUW4O|UoRjJ3NUW1PFc2RC:jPh?=
HepG2  Ml\TSpVv[3Srb36gRZN{[Xl? M4rQdVExKM7:TR?= MX:1JIg> M4rOR4Jtd2OtczDwbI9{eGixconsZZRm\CCPQWDLd{BqdmS3Y3XkJIJ6KGW6b3flco92eyCWR1[t{tIy NHjmT489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW2NFQ5QCd-MkW1OlA1QDh:L3G+
MCF-7  NXS4TolMTnWwY4Tpc44hSXO|YYm= MYCxNEDPxE1? MlftNUBp M{TGcYlvcGmkaYTzJGlNNTF6LXXubIFv[2WmIHPlcIwhdWmpcnH0bY9v MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd{N{CxNUc,OjV5MkewNVE9N2F-
H1355 NFHyPJZHfW6ldHnvckBCe3OjeR?= MXSyOUDPxE1? MoS3NUBp MXjicJVvfHNidHjlJGJc[V2SLXnu[JVk\WRiaX7jdoVie2ViaX6gdIhwe3Cqbz3DbIsyKGGwZDDwbI9{eGixLVXST{BmgHC{ZYPzbY9v MmDNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5NkmxPFEoRjJ3N{[5NVgyRC:jPh?=
PC12 NXn2WYxxTnWwY4Tpc44h[XO|YYm= MljpTY5pcWKrdHnvckBw\iCwZYL2[UBoem:5dHig[oFkfG:{LX3l[IlifGWmIF3BVEBscW6jc3WgZYN1cX[rdImgbY4hcHWvYX6gVGMyOiClZXzsd{whUUN3MDC9JFIh|ryPLh?= MoraQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThyN{ezOlMoRjF6MEe3N|Y{RC:jPh?=
HT-29 NFnDTpVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NI\VXoU2KGSjeYO= M1zZ[WFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSGStNlkh[2WubIOgZYZ1\XJiNTDkZZl{KGK7IGfTWFEh[XO|YYmsJGlEPTBiPTC0JO69VS5? M1nBdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEe4N|E3Lz5{NUC3PFMyPjxxYU6=
IEC6 NGrXOFNHfW6ldHnvckBie3OjeR?= MYC1JI1qdnN? NWKwOIljUW6qaXLpeIlwdiCxZjDNSWsyNzJiaX6gdoF1KEmHQ{[gZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKEWUS{GvNkBtd2:yIIDoc5NxcG:{eXzheIlwdiCmb4Pl[EA{OCCvaX7zJIJm\m:{ZTD0c{B{fGmvdXzheIlwdiC5aYToJFExLSC|ZYL1cUBnd3JiNTDtbY5{KGK7IHntcZVvd2Kub4T0bY5oKG2ndHjv[EwhUUN3MDC9JFQvOiEQvF2u M3LwNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEe4N|E3Lz5{NUC3PFMyPjxxYU6=
NG 108-15 M3XxRmZ2dmO2aX;uJIF{e2G7 Mn33R49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDhZo9tcXOqIF3BVGsh[WO2aY\peJkhcW5ibX;1d4UhdmW3cn;icIF{fG:vYTDhcoQhemG2IHfsbY9u[SCqeXLybYQhVkdiMUC4MVE2KGOnbHzzMEBC[3Srdnn0fUA:KDFyIN88UU4> M1XyclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NUO3N|U1Lz5zNUWzO|M2PDxxYU6=
PC12 NHT3XWFHfW6ldHnvckBie3OjeR?= NHj0OWsyOCC3TR?= MWG1JIhzew>? NUL3VVJjSWO2aY\heIlwdiCxZjDOdoYzN0GURTDpckBz[XRiUFOxNkBk\WyuczDhd5Nme3OnZDDhd{BJVy1zIIDyc5RmcW5iaX7keYN1cW:wIHH0JFExKHWPIHHmeIVzKDViaILzJJBz\XS{ZXH0[YQhf2m2aDDKUmshcW6qaXLpeI9zKFOSNkCwNVI2KG[xcjCxJIhzKGKnZn;y[UBkd22yb4Xu[EBi\GSrdHnvckBjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= NIDIe|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUO0OVY5PSd-MkGzOFU3QDV:L3G+
PC12 M3fTUWZ2dmO2aX;uJIF{e2G7 NHnjT|YyOCC3TR?= NIi2N4k2KGi{cx?= NYHtTY5TSWO2aY\heIlwdiCxZjDOdoYzN0GURTDpckBz[XRiUFOxNkBk\WyuczDhd5Nme3OnZDDhd{BJVy1zIIDyc5RmcW5iaX7keYN1cW:wIHH0JFExKHWPIHHmeIVzKDViaILzJJBz\XS{ZXH0[YQhf2m2aDDNSWszKGmwaHnibZRweiCXMEGyOkBnd3JiMTDodkBj\W[xcnWgZ49ueG:3bnSgZYRlcXSrb36gZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| M4\KNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{S1Olg2Lz5{MUO0OVY5PTxxYU6=
PC12 NF3OcI9HfW6ldHnvckBie3OjeR?= NUHkfVZQOTBidV2= M{Lp[VUhcHK| M4nyb2FkfGm4YYTpc44hd2ZiToLmNk9CWkViaX6gdoF1KFCFMUKgZ4VtdHNiYYPz[ZN{\WRiYYOgTG8uOSCycn;0[YlvKGmwZIXjeIlwdiCjdDCxNEB2VSCjZoTldkA2KGi{czDwdoV1emWjdHXkJJdqfGhiTVXLNUBqdmirYnn0c5IhWER7OEC1PUBnd3JiMTDodkBj\W[xcnWgZ49ueG:3bnSgZYRlcXSrb36gZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| M4q1WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{S1Olg2Lz5{MUO0OVY5PTxxYU6=
HeLa MU\GeY5kfGmxbjDhd5NigQ>? NYjq[3JmOjBidV2= NFi2TW4{KGi{cx?= MVfJcohq[mm2aX;uJI9nKEWUS{GvNkBxcG:|cHjvdplt[XSrb36gbY4hcHWvYX6gTIVN[SClZXzsd{BifCB{MDD1UUBi\nSncjCzJIhzeyCkeTDX[ZN1\XKwIHLsc5R1cW6pIHHuZYx6e2m| Mlf2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3N{C2NVUoRjJ|NUewOlE2RC:jPh?=
RD MWjxTHRUKGG|c3H5 NV62UpZkeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLEJINmdGy| NV6yXIo1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB1643 MWXxTHRUKGG|c3H5 MnzLdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6EMU[0N{Bk\Wyucx?= NXXjSoJ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
RD NH7VfYNyUFSVIHHzd4F6 MnnXdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVmQh[2WubIO= NWH3d2F[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC MkjWdWhVWyCjc4PhfS=> MXnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= MoT1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
TC32 NX[xS5Q2eUiWUzDhd5NigQ>? NILieZZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBVSzN{IHPlcIx{ MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
MDA-MB-231 M4\0UGZ2dmO2aX;uJIF{e2G7 MYS1NEB2VQ>? MYPEc5dvemWpdXzheIlwdiCxZjDNUXAzKGmwIHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[XRiNUCgeW0h[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz NXXGO|FyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5NlYzOjZpPkKyPVI3OjJ4PD;hQi=>
MDA-MB-231 NV\EXZd7TnWwY4Tpc44h[XO|YYm= MofIOVAhfU1? MVrEc5dvemWpdXzheIlwdiCxZjDNUXA6KGmwIHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[XRiNUCgeW0h[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz NHHyOXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyOlIzPid-MkK5NlYzOjZ:L3G+
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Methods Test Index PMID
Western blot c-Jun / α-tubulin / p-ERK / ERK / p-AKT / AKT ; p-JNK / JNK / Cyclin D1 ; p-HER2 / HER2 ; MMP9 / XIAP / VEGF 17482134 24244023 29695559
体内試験

Treatment of mice 30 minutes before focal cerebral ischemia with PD98059 protects against damage, resulting in a decrease in infarct volume. [5] Pretreated with PD98059 (10 mg/kg per i.v. injection) 30 minutes before and then together with hourly cerulein injections for 3 hours significantly ameliorates cerulein-induced acute pancreatitis ipancreatitis on the basis of pancreatic wet weight and histology. [6] Administration of PD98059 (10 mg/kg) in mice 1 hour after carrageenan causes a reduction in all the parameters of inflammation measured. [7]

お薦めの試験操作(参考用のみ)

キナーゼ試験:

[1]

  • In vitro MEK-inhibitory activity:

    Incorporation of 32P into myelin basic protein (MBP) is assayed in the presence of glutathione S-transferase (GST) fusion proteins containing the 44-kDa MAPK (GST-MAPK) or the 45-kDa MEK (GST-MEK1). Assays are conducted in 50 μL of 50 mM Tris, pH 7.4/10 mM MgCl2/2 mM EGTA/10 μM [γ-32P]ATP containing 10 μg of GST-MEK1, 0.5 μg of GST-MAPK, and 40 μg of MBP. After incubation at 30°C for 15 minutes, reactions are stopped by addition of Laemmli SDS sample buffer. Phosphorylated MBP is resolved by SDS/10% PAGE.

細胞試験:

[1]

  • 細胞株:K-Balb, KNRK, v-raf-3Y1, SRA/3Y1, EGFR/3T3, and K562
  • 濃度: Dissolved in DMSO, final concentrations ~100 μM
  • 反応時間:3 dyas, or 7-10 days
  • 実験の流れ:

    For monolayer growth, cells are plated into multi-well plates at 10,000-20,000/mL. Forty-eight hours later, various concentrations of PD98059 are added to the cell growth medium and incubation is continued for an additional 3 days. Cells are then removed from the wells by incubation with trypsin and enumerated with a Coulter Counter. For growth in soft agar, cells are seeded into 35-mm dishes at 5,000-10,000 cells per dish with growth medium containing 0.3% agar and desired concentrations of PD98059. After 7-10 days of growth, visible colonies are manually enumerated with the aid of a dissecting microscope.

動物試験:

[7]

  • 動物モデル:Male Sprague–Dawley rats with acute pancreatitis
  • 投薬量:10 mg/kg
  • 投与方法:Injection i.v.

溶解度 (25°C)

体外

体内

左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
4% DMSO+30% PEG 300+5% Tween 80+ddH2O
混合させたのち直ちに使用することを推奨します。

1mg/mL

化学情報

分子量 267.28
化学式

C16H13NO3

CAS No. 167869-21-8
Storage 3年 -20°C
2年 -80°C in solvent
Smiles COC1=CC=CC(=C1N)C2=CC(=O)C3=CC=CC=C3O2

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

便利ツール

質量 濃度 体積 分子量

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

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よくある質問(FAQ)

質問1:
How to formulate this inhibitor for i.p. injection?

回答
You can prepare the stock by the vehicle 30% PEG400/0.5% Tween80/5% Propylene glycol, 0.5% CMC.

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