PD98059

For research use only. Not for use in humans.

製品コードS1177

PD98059化学構造

CAS No. 167869-21-8

PD98059 is a non-ATP competitive MEK inhibitor with IC50 of 2 μM in a cell-free assay, specifically inhibits MEK-1-mediated activation of MAPK; does not directly inhibit ERK1 or ERK2. PD98059 is a ligand for the aryl hydrocarbon receptor (AHR) and functions as an AHR antagonist.

サイズ 価格(税別)  
10mM (1mL in DMSO) JPY 24200
JPY 26400
JPY 80000
JPY 113200
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文献中Selleckの製品使用例(414)

製品安全説明書

MEK阻害剤の選択性比較

生物活性

製品説明 PD98059 is a non-ATP competitive MEK inhibitor with IC50 of 2 μM in a cell-free assay, specifically inhibits MEK-1-mediated activation of MAPK; does not directly inhibit ERK1 or ERK2. PD98059 is a ligand for the aryl hydrocarbon receptor (AHR) and functions as an AHR antagonist.
特性 Does not inhibit c-Raf phosphorylated MEK1.
ターゲット
AhR [1]
(Cell-free assay)		 MEK1 [1]
(Cell-free assay)
1 μM 2 μM
体外試験

PD98059 inhibits either basal MEK1 or a partially activated MEK produced by mutation of serine at residues 218 and 222 to glutamate (MEK-2E) with IC50 of 2 μM. PD98059 does not inhibit the MAPK homologues JNK and P38. PD98059 is highly selective against MEK, as it does not inhibit a number of other kinase activities including Raf kinase, cAMP-dependent kinase, protein kinase C, v-Src, epidermal growth factor (EGF) receptor kinase, insulin receptor kinase, PDGF receptor kinase, and phosphatidylinositol 3-kinase. PD98059 inhibits PDGF-stimulated activation of MAPK and thymidine incorporation into 3T3 cells with IC50 of ~10 μM and ~7 μM, respectively. [1] PD98059 potently prevents the activation of MEK1 by Raf or MEK kinase with IC50 of 4 μM, and weakly inhibits the activation of MEK2 by Raf with IC50 of 50 μM. PD98059 does not inhibit the activation of MEK homologues MKK4 and RK kinase that participate in stress and interleukin-1-stimulated kinase cascades in KB and PC12 cells, and the activation of p70 S6 kinase by insulin or epidermal growth factor in Swiss 3T3 cells. [2] PD98059 completely blocks the nerve growth factor (NGF)-induced differentiation of PC12 cells without altering cell viability. [3] PD98059 inhibits the proliferation of RAW264.7 cells in the culture containing RANKL in a dose-dependent manner, resulting in an apparent decrease of TRAP-positive cells. [4]
細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A2780  NFXSUZFHfW6ldHnvckBCe3OjeR?= MX2yNOKh|ryP M4S1V|EhcA>? NInlPJljdG:la4OgSHRETC2rbnT1Z4VlKESUNTDlfJBz\XO|aX;u MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ7Nki2Nkc,OjN4OU[4OlI9N2F-
AML 1# NIPXV3RCeG:ydH;zbZMhSXO|YYm= NE\W[YQzOMLizszN NV\oSnJXOTJiaB?= M3z6SYVvcGGwY3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgV|E> NWLEWmpKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFY3QTFpPkKzO|A3PjlzPD;hQi=>
KG-1  MWHBdI9xfG:|aYOgRZN{[Xl? Ml[wNlDDqM7:TR?= MVuxNkBp MlfD[Y5p[W6lZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDTNS=> MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzdyNk[5NUc,OjN5ME[2PVE9N2F-
HUVECs NH7TSmxCeG:ydH;zbZMhSXO|YYm= Mm\SNk81KM7:TR?= MUOyOE81QCCq NH\Re|dqdmS3Y3XzJINmdGxiZHXheIg> Mly0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5MEe1NlAoRjJ|N{C3OVIxRC:jPh?=
HepG2  MVnGeY5kfGmxbjDBd5NigQ>? NEG2SY8zOMLizszN MkjlNlQhcA>? M{Pkd4lvcGmkaYTzJJRp\SCKTz2xJJBzd3SnaX6g[ZhxemW|c3nvckBkdy22cnXheIVlKHerdHigcYV1\m:{bXnu Ml7uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5MEe2NFkoRjJ|N{C3OlA6RC:jPh?=
NB4  M1jpW2Fxd3C2b4Ppd{BCe3OjeR?= NGfKcJoyOC9{MD:2NEDPxE1? M4rDTVEvPSCq M2PHR2ROW09? NUj4fo0y\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JINwNXS{ZXH0[YQhf2m2aDDQZYNtcXSjeHXs M4HJPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{O1OVQyLz5{M{ezOVU1OTxxYU6=
BxPC-3 cells NGj0N4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXz1RmNUOjEEoN88US=> NYfObnlEOC53IHi= NGXydXZqdmirYnn0d{BXTUeILVGtdoVofWyjdHXkJGhWXkWFIHfyc5d1cCCjbnSgeJVj\SCob4LtZZRqd25iaX7keYNm\CCkeTDQRXIuOiCDUB?= NV7TNYNyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3OlQxPDZpPkKzO|Y1ODR4PD;hQi=>
MKN45 MkPFRZBweHSxc3nzJGF{e2G7 MWexNEDPxE4EoB?= Mm\lNlQhcA>? NEDme2JqdmO{ZXHz[ZMhfGinIFTBVHQucW6mdXPl[EBk\WyuIHHwc5B1d3Orcx?= NHfsbFQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e5NlU5QCd-MkO3PVI2QDh:L3G+
SGC7901 NEHLPG9CeG:ydH;zbZMhSXO|YYm= M{jYRVExKM7:TdMg NH\BZ2czPCCq Mm\ybY5kemWjc3XzJJRp\SCGQWDUMYlv\HWlZXSgZ4VtdCCjcH;weI9{cXN? NUL6UWp2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PVI2QDhpPkKzO|kzPTh6PD;hQi=>
MKN45 NVLPSHlUTnWwY4Tpc44hSXO|YYm= NF;3NYwyOCEQvF5CpC=> NGDQSHkzPCCq NWS1cHpqcW6qaXLpeJMhfGinIHX4dJJme3Orb36gc4YheGixc4Doc5J6dGG2ZXSgSXJMOS9{ NX22NYtqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PVI2QDhpPkKzO|kzPTh6PD;hQi=>
SGC7901 M{\Semdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M374dVExKM7:TdMg M4L2cVI1NzR6L{eyJIg> NX:1cpBvcW6qaXLpeJMh[2WubDDndo94fGhiY3:teJJm[XSnZDD3bZRpKESDUGS= MmDyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUK1PFgoRjJ|N{myOVg5RC:jPh?=
MKN45 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGLnVnoyOCEQvF5CpC=> NWfUdmlGOjRxNEivO|IhcA>? MWPpcohq[mm2czDj[YxtKGe{b4f0bEBkdy22cnXheIVlKHerdHigSGFRXA>? NUjDNII5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PVI2QDhpPkKzO|kzPTh6PD;hQi=>
SGC7901 MXvGeY5kfGmxbjDBd5NigQ>? MkjWNVAh|ryPwrC= NF70OVkzPCCq NWexOIdRcW6qaXLpeJMhfGinIHX4dJJme3Orb36gc4YheGixc4Doc5J6dGG2ZXSgSXJMOS9{ NUDUclc6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PVI2QDhpPkKzO|kzPTh6PD;hQi=>
HUASMCs Ml3zSpVv[3Srb36gRZN{[Xl? NEHsS3AyOCEQvF5CpC=> M3Hmc|I1KGh? NX;IXmxj\GmvaX7pd4hmeyCDbnegTWku[2G3c3XkJHNQS1N|IH3SUmEh[W6mIIDyc5RmcW5iZYjwdoV{e2mxbtMg NGX5ZWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ixOlQ3QCd-MkO4NVY1Pjh:L3G+
HUASMCs MWLGeY5kfGmxbjDBd5NigQ>? MWmxNEDPxE4EoB?= M{W1bFI1KGh? MV7pcohq[mm2czDBcochUUlvaX7keYNm\CCHUluxM|IheGixc4Doc5J6dGG2aX;uJIxmfmWu M2HDW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEG2OFY5Lz5{M{ixOlQ3QDxxYU6=
EPOR/CR3 MYHGeY5kfGmxbjDBd5NigQ>? MVS1NOKh|ryP NHPlToY{KGh? NXjqeWZYemWmdXPld{BGWE9iYX7kM49zKEmOLUOtbY5lfWOnZDD0bIUhfHm{b4PpcoUheGixc4Doc5J6dGG2aX;uxsA> MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{MEezNUc,OjN6MkC3N|E9N2F-
NB4  M2HiU2Z2dmO2aX;uJGF{e2G7 NV7G[FRROTBizszNxsA> NEjYUIc4OiCq MXLEUXNQ MUPpcohq[mm2czDkZZNifGmwaXKtbY5lfWOnZDDDSFEy[iCneIDy[ZN{cW:w NFfneo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iyOVU5PSd-MkO4NlU2QDV:L3G+
HL60  NHP6Z5pHfW6ldHnvckBCe3OjeR?= MoTSNlDDqM7:TR?= NYXCeGM5PzJiaB?= MV3EUXNQ M1jPVolvcGmkaYTzJIRie2G2aX7pZk1qdmS3Y3XkJGNFOTGkIHX4dJJme3Orb36= M4m2fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEK1OVg2Lz5{M{iyOVU5PTxxYU6=
HUVECs NGC0[nFHfW6ldHnvckBCe3OjeR?= MVeyOUDPxE1? Mm\rNUBp M{f2Nolv[3KnYYPlJG5HNc78QjDwOlUhdnWlbHXhdkB1emGwc3zvZ4F1cW:w M3;6NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUCxNFA5Lz5{M{mwNVAxQDxxYU6=
LNCaP  MYfGeY5kfGmxbjDBd5NigQ>? NV3iT5dJOTBizszNxsA> M{nmU|EhcA>? NHmxUG5l\WO{ZXHz[ZMhfGinIFXHSkB2eHKnZ4XsZZRm\CCyLWnCMVE> NYHpXIVSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4N|g{OThpPkKzPFM5OzF6PD;hQi=>
HL-60 M4nrNWFxd3C2b4Ppd{BCe3OjeR?= MVS1NOKh|ryP MkTXNUBp NFL1SVBz\XOldXXzJGJCOTR3IH3l[IlifGWmIHHwc5B1d3Orcx?= NYTZNppmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5OFg4PTFpPkKzPVQ5PzVzPD;hQi=>
HepG2 MVLGeY5kfGmxbjDBd5NigQ>? MYS0NEDPxE1? M4LEUFYwOTJiaB?= M4f5NolvcGmkaYTzJJRp\SCrbnPy[YF{\SCxZjDwMWVTUzFiYX7kJJAu[y2MdX6gdJJwfGWrbjDlfJBz\XO|aX;uJIJ6KFCO NGrpUVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m0Nlg2OSd-MkO5OFI5PTF:L3G+
HPAF-II NF3ze3NHfW6ldHnvckBCe3OjeR?= MoLmNVAh|ryPwrC= MYm2JIg> MknubY5kemWjc3WgcYlTNTF2MzDlfJBz\XO|aX;u MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl5M{exNEc,OjN7N{O3NVA9N2F-
BxPC-3 Mni0SpVv[3Srb36gRZN{[Xl? NF;McXQyOCEQvF5CpC=> NIiyNpk3KGh? MXLpcoNz\WG|ZTDtbXIuOTR|IHX4dJJme3Orb36= M{S5R|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUezO|ExLz5{M{m3N|cyODxxYU6=
COLO205 MUnBdI9xfG:|aYOgRZN{[Xl? M3X3WFExNzJyL{SwJO69VQ>? MWeyOEBp MmnKbY5lfWOnczDEUmEhdGGmZHXyJIZwem2jdHnvci=> Ml3NQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUmxNFgoRjJ2MEG5NVA5RC:jPh?=
G292  M1LqTGFxd3C2b4Ppd{BCe3OjeR?= M2DwOVMxyqEQvF2= MVOyJIg> Mlr0doV{fG:{ZYOgZ4Fxe2GrY3nuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NWHxTGVTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNVI6OzBpPkK0NFEzQTNyPD;hQi=>
SGC7901 NF35[lBCeG:ydH;zbZMhSXO|YYm= M2nuTVIxyqEQvF2= MWSxJIg> M4TySYlvcGmkaYTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEmITj5OteKh[W6mIEZihNIuTE[XUh?= M2P6bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEK3O|UxLz5{NECyO|c2ODxxYU6=
MGC803  MnvsRZBweHSxc3nzJGF{e2G7 M1qxPFIxyqEQvF2= NWPLc2RCOSCq NI\DOnhqdmirYnn0d{BieG:ydH;zbZMhcW6mdXPl[EBjgSCLRl6t{tHDqGGwZDC15qCzNUSIVWK= MnXyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMke3OVAoRjJ2MEK3O|UxRC:jPh?=
HPMC MmmwRZBweHSxc3nzJGF{e2G7 M2rhUlExyqEQvF2= NX;5PY56OjUEoHi= NIC4dYtz\X[ncoPld{Bl\WO{ZXHz[UBqdiClZXzsJJZq[WKrbHn0fUBqdmS3Y3XkJIJ6KEiJUFTT M2\ESFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MESyPFM5Lz5{NEC0Nlg{QDxxYU6=
HCT-8 NILacohCeG:ydH;zbZMhSXO|YYm= MmnZNVDDqM7:TR?= MUSyOOKhcA>? M4DwN4Rm[3KnYYPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JFUuTlV? M{jZW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEm1PFY{Lz5{NEC5OVg3OzxxYU6=
HPMC MoHDSpVv[3Srb36gRZN{[Xl? MV:xNOKh|ryP MWO0PEBp MoDqdoV3\XK|ZYOgeIhmKGOqYX7n[ZMhcW5iY3XscEBud3KyaH;sc4d6KGmwZIXj[YQh[nliSFfQSHM> MmXXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyNEK4N|goRjJ2MESyPFM5RC:jPh?=
A549 MkHqSpVv[3Srb36gRZN{[Xl? NXT6ZodLPTEEoN88US=> NXPibXgzOiCq NI\pV2ZjdG:la4OgSXJMKHCqb4PwbI9zgWyjdHnvckBu\WSrYYTl[EBjgSBzLEKtUnE> NUTvXGhkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwOlc4OjdpPkK0NFY4PzJ5PD;hQi=>
Caco-2  NIHzNWJCeG:ydH;zbZMhSXO|YYm= MknhNVDDqM7:TR?= MXKyOOKhcA>? NXLMTmVE\GWlcnXhd4V{KGOnbHygZZBweHSxc3nzJIlv\HWlZXSgZpkhPS2IVR?= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7NUi2N{c,OjRyOUW4OlM9N2F-
AGS NYD2ZYV1TnWwY4Tpc44hSXO|YYm= MUSxNEDPxE4EoB?= M4XmO|AvPSCq M{\YV4lvcGmkaYTzJJRp\SC3cILl[5Vt[XSrb36gc4YhfGinIFnMMVgh\2WwZR?= NFewWZo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGwOlE3Pid-MkSxNFYyPjZ:L3G+
Ca9-22 MVXGeY5kfGmxbjDBd5NigQ>? M1fJUFMh|ryP M{j2SFEwOiCq M1HKVJJm\HWlZYOgTIJTNWmwZIXj[YQhSVSILUKgdIhwe3Cqb4L5cIF1cW:w M1ToR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUK2OVMzLz5{NEGyOlU{OjxxYU6=
Ca9-22 M13kSWZ2dmO2aX;uJGF{e2G7 MlSwN{DPxE1? NHnt[IQyKGh? M3HJTIFjd2yrc3jld{B1cGViYXLpcIl1gSCxZjDIZnIhfG9iaX7keYNmKEmOLUigdJJw\HWldHnvci=> M4nRN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUK2OVMzLz5{NEGyOlU{OjxxYU6=
HAECs NXXGNmtKTnWwY4Tpc44hSXO|YYm= M{fVXFExKM7:TR?= NGXjcGYyKGh? MUJCpIF1fGWwdXH0[ZMhXE6ILd8xMZN1cW23bHH0[YQhUUODTT2xJIFv\CCYQ1HNMVEh\XiycnXzd4lwdg>? M4TWNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUO0OlU4Lz5{NEGzOFY2PzxxYU6=
Caco-2 M2LQfmZ2dmO2aX;uJGF{e2G7 M2D2XVUxyqEQvF2= MUO0PIg> MmH1SG1UVw>? MWnlcohidmOnczD0bIUhdVKQQTDs[ZZmdHNib3dCpHNEVk5zQTzGXHlFOy{EoFzDWEzDqEyRWD|CpGhKTjODLNMgXmcyPi{EoGDESVZCyqCjbnVCpGxISUyVMUdCpIdmdmW|wrDjc{11emWjdHXkJJdqfGhiRHX4 NUnvUHR3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOlE3QTVpPkK0NVYyPjl3PD;hQi=>
MCF-7 Mly2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYKxNEDPxE1? MlL1OFhp M3rKdGROW09? Mk\PdoV3\XK|ZYOgRm5HNWmwZIXj[YQh[2WubDDjfYNt\SCjcoLld5Q> M3SzfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MU[zOFA1Lz5{NEG2N|QxPDxxYU6=
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SGC7901  MXfBdI9xfG:|aYOgRZN{[Xl? M3S2bFUxyqEQvF2= NWH0[o9iOjRxNEivO|IhcA>? M3PyN2ROW09? M2nPVYlv\HWlZYOgZZBweHSxc3nzJINwdWKrbnXkJJdqfGhiSlHLNkB{cFKQQR?= NFn1T4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG3PFI1OCd-MkSxO|gzPDB:L3G+
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DLD-1  MVPGeY5kfGmxbjDBd5NigQ>? MVKyNOKh|ryP MX60PEBp M3PTSpJm\HWlZYOgeIhmKEKQSWCzJIV5eHKnc4Ppc44heHKnLYTy[YF1\WRid3n0bEA2NWG8YT3kRy=> MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJzMUW4NUc,OjR{MUG1PFE9N2F-
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KATOIII  NGDZTXNHfW6ldHnvckBCe3OjeR?= Ml;4OVAh|ryP MVu0PEBp MXPEUXNQ M{nUZolv[3KnYYPld{BmgHC{ZYPzbY9vKG:oIFjMRU1CODJib4KgTGxCNUF{NDDtc4xm[3WuZYO= NYTVfZljRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
NCI-N87  NELZNY9HfW6ldHnvckBCe3OjeR?= Mn7mOVAh|ryP MnHWOFghcA>? NFv1VGJFVVOR NVfiZZZScW6lcnXhd4V{KGW6cILld5Nqd25ib3[gTGxCNUFyMjDvdkBJVEFvQUK0JI1wdGWldXzldy=> NXvJXFY4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
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KYSE30 M4rpPWZ2dmO2aX;uJGF{e2G7 NWXt[ppPPTBizszN NHrqOFY1QCCq NVzmOZBITE2VTx?= NVW5NlNyfXC{ZXf1cIF1\XNidHjlJIV5eHKnc4Ppc44hd2ZiSFzBJINt[XO|IFm= NVLhXVh{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
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HT29 NF3TOnVHfW6ldHnvckBCe3OjeR?= M4PqTlExKM7:TR?= MnLoNkBp MVzpcohq[mm2czDv[kBLSUt{LDDFVmsyNzJiYX7kJHNVSVR|IIDoc5NxcG:{eXzheIlwdg>? NXX4T4ZLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOlUzQTNpPkK0NlY2Ojl|PD;hQi=>
HepG2 Mk[4RZBweHSxc3nzJGF{e2G7 MmnQNlAh|ryP M2fPfVI1KGh? NHXwW2hqdmirYnn0d{BGWktzL{KgdIhwe3Cqb4L5cIF1cW:wIHHu[EBmdmijbnPld{BXSjFvaX7keYNm\CCjcH;weI9{cXN? M1nxVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkS3PVA6Lz5{NEK0O|kxQTxxYU6=
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MC-3 MkfSRZBweHSxc3nzJGF{e2G7 NFvQNpEyOCEQvF2= M3T5U|I1KGh? M33PbJBwfGWwdHnheIVlKE2HU1OtbY5lfWOnZDDhdI9xfG:|aYOgbY4hKGOnbHzz NVvvR45wRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyO|A2OjNpPkK0NlcxPTJ|PD;hQi=>
Raji  NHfhWFhHfW6ldHnvckBCe3OjeR?= MVuxNEDPxE1? NHnUNogyKGh? MWnicI9kc3NiaIPCRWZHKGmwZIXj[YQhTXKtMT:yJJBpd3OyaH;yfYxifGmxbh?= NGq4eYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK2PVY{OCd-MkSyOlk3OzB:L3G+
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PANC-1 MV\GeY5kfGmxbjDBd5NigQ>? MlfzNlAh|ryP NFfwV5M1QCCq MlHZbY5pcWKrdIOgeIhmKGW6cILld5Nqd25ib3[g{rQ3TCCrbjDy[ZNxd26|ZTD0c{B1cGViUGDBVu61yqCjZ3;ubZN1yqB? NYHjbHpURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPVQyOzNpPkK0Nlk1OTN|PD;hQi=>
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SW480 M4freGZ2dmO2aX;uJGF{e2G7 NEjYfG0yOCEQvF2= MlrENlAhcA>? MWDEUXNQ M3Sw[ZN2eHC{ZYPz[ZMhfGinIFPSWEBi[3Srdnn0fS=> M{nzcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{K0N|Y3Lz5{NEOyOFM3PjxxYU6=
HEK 293 MkLQSpVv[3Srb36gRZN{[Xl? MmXHNVAh|ryP M2\Ie|UhcA>? MWDEUXNQ MXjpcohq[mm2czDXcpQucW6mdXPl[EDPui2lYYTlcolvN1SFRkSgZYN1cX[rdImgZY5lKG63Y3zlZZIh|rJvY3H0[Y5qdiCjY3P1cZVt[XSrb36= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN{NEO2Okc,OjR|MkSzOlY9N2F-
HEK 293 MXzGeY5kfGmxbjDBd5NigQ>? Ml24NVAh|ryP Mn:yOUBp MkjTSG1UVw>? MUXzeZBxemW|c3XzJJRp\SCFUmSgZYN1cX[rdIm= NFHHe|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOyOFM3Pid-MkSzNlQ{PjZ:L3G+
HL-60  NHTFUINHfW6ldHnvckBCe3OjeR?= MXSxNE8zOCEQvF2= M4fPO|EhcA>? Mm\4bY5pcWKrdIOgeIhmyqCQLjDjbIlv\W6|aYPlfJRz[WO2wrDpcoR2[2WmIHTp[oZmemWwdHnheIlwdiCrboTvJIdz[W63bH;jfZRmew>? NUDrVnE6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOVcxOjBpPkK0N|U4ODJyPD;hQi=>
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HeLa M4PCOmZ2dmO2aX;uJGF{e2G7 NVjVdHNrPTBizszN MlPJNE42KGh? NX\ROFlR[myxY3vzJHRTYC1zIH71Z4xm[XJibXnndoF1cW:wIHHu[EBVYE6LUDDkc5dvNXKnZ4XsZZRqd25? M4PCbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{e2PFI4Lz5{NEO3OlgzPzxxYU6=
HUVECs M1vjZWZ2dmO2aX;uJGF{e2G7 MWexNOKh|ryP NHzVS24yKGh? M1rWdIlvcGmkaYTzJJRp\SCKRFygdoVlfWOnZDDDU3guOiCneIDy[ZN{cW:wIHHu[EBRT0lvMjDy[Yxm[XOn M{fuTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{i1NVA6Lz5{NEO4OVExQTxxYU6=
MCF-7 NFnEd4NHfW6ldHnvckBCe3OjeR?= MWixNOKh|ryP NGrWToYyOC9|MDDtbY4> MUXy[YR2[2W|IITo[UBWXFBvZHXw[Y5l\W62IFXST{BxcG:|cHjvdplt[XSrb36= M1;3NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{mwPFE6Lz5{NEO5NFgyQTxxYU6=
HGC-27 NXfqbpNySXCxcITvd4l{KEG|c3H5 MoPnNUDDvU1? NHXPZ4MyKGh? NEf0WIR{fXCycnXzd4V{KFKDRECwNUBxdHW|IF3LMVIzODZvaX7keYNm\CClZXzsJJZq[WKrbHn0fUBtd3O| M37UZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEG2N|Q6Lz5{NESxOlM1QTxxYU6=
PC3  MXrBdI9xfG:|aYOgRZN{[Xl? M1rYT|UxKM7:TR?= MWmwMlUhcA>? NGTnNndqdmirYnn0d{BOUFlvNES5MYlv\HWlZXSgZZBweHSxc3nzxsA> NHXoXXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NESyOFg5QSd-MkS0NlQ5QDl:L3G+
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A375 MofNR4VtdCCLbo\hd4lwdiCDc4PhfS=> MorrNVDjiJN{MDFCuW0> NYHOdnJ1OjRiaB?= M{LvRpJm\HWlZYOgcYVt[W6xbXGgZ4VtdCCrbo\hd4lwdg>? NYnRb|YyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OlYxOzZpPkK0OFY3ODN4PD;hQi=>
HBMEC NEjIeWJHfW6ldHnvckBCe3OjeR?= MXKxNEDPxE1? NVzWNHY3OSCq MXPicI9kc3NiVlXHSk1qdmS3Y3XkJGVxcEF{IHX4dJJme3Orb36= NWjySJoyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OVg6QDJpPkK0OFU5QTh{PD;hQi=>
HCT-15 MWPGeY5kfGmxbjDBd5NigQ>? NFjpeIIyKGh? NVP3dXdi[XS2ZX71ZZRmeyCSR1WyMYlv\HWlZXSgdIhwe3Cqb4L5cIF1cW:wIH;mJGVzc8Li MnTQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{G0NlUoRjJ3NEOxOFI2RC:jPh?=
HCT-15 M4r4c2Fxd3C2b4Ppd{BCe3OjeR?= M4nH[lEhcA>? NHTQOYVi[m:uaYPo[ZMhfGinIIDyc5Rm[3SrdnWg[YZn\WO2czDv[kBRT0V{wrDh[4FqdnO2IHP1doN2dWmwLXnu[JVk\WRiYYDvdJRwe2m| MoDxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{G0NlUoRjJ3NEOxOFI2RC:jPh?=
786-O NF:yenFCeG:ydH;zbZMhSXO|YYm= NEXXZ4U2OMLizszN M2\rOVI1KGh? M361RZBwfGWwdHnheIV{yqC2aHWgdJJwNWGyb4D0c5Rq[yCnZn\lZ5R{KG:oIF7D NF\VSJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWwPFQ4Pid-MkS1NFg1PzZ:L3G+
HepG2  NF3XNXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXfRRlNqOjBizszN NY\vNWNxOjRiaB?= NETxSFZ{fXCycnXzd4V{KFSJRj5OtlEucW6mdXPl[EBk\WyuIIDyc4xq\mW{YYTpc44h[W6mIHnueoF{cW:w M3fUS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NU[wOFg5Lz5{NUW2NFQ5QDxxYU6=
SW480 M1jVfGZ2dmO2aX;uJGF{e2G7 M4PjclIxyqEQvF2= NWfjOJd5OcLiaB?= MnfhdoVlfWOnczD0bIUh\XiycnXzd4lwdiCxZjDBWGY{KHC{b4TlbY4> NXHEW4Z{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OFc5OTZpPkK1OFQ4QDF4PD;hQi=>
MDA-MB-231 MV3GeY5kfGmxbjDBd5NigQ>? M3LLN|I2KM7:TR?= NF\YNpczNTNiaB?= M2nMU4Rm[3KnYYPld{BxNUWUS{GvNkBidmRiU{GwNGE1KGW6cILld5Nqd25? M3\SU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUW1PFc2Lz5{NUW1OVg4PTxxYU6=
HepG2  NFHR[odHfW6ldHnvckBCe3OjeR?= MWKxNEDPxE1? MXq1JIg> Ml3UZoxw[2u|IIDoc5NxcG:{eXzheIVlKE2DUFvzJIlv\HWlZXSgZpkh\XixZ3Xuc5V{KFSJRj5OtlE> MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV4MES4PEc,OjV3NkC0PFg9N2F-
MCF-7  MYnGeY5kfGmxbjDBd5NigQ>? M4XrSFExKM7:TR?= MWexJIg> MlzzbY5pcWKrdIOgTWwuOThvZX7oZY5k\WRiY3XscEBucWe{YYTpc44> MmXmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5MkewNVEoRjJ3N{K3NFEyRC:jPh?=
H1355 NFzkUmtHfW6ldHnvckBCe3OjeR?= NXvreXc{OjVizszN NGPJbowyKGh? M2LyV4JtfW62czD0bIUhSlujXWCtbY5lfWOnZDDpcoNz\WG|ZTDpckBxcG:|cHjvMWNpczFiYX7kJJBpd3OyaH:tSXJMKGW6cILld5Nqd25? MkHUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5NkmxPFEoRjJ3N{[5NVgyRC:jPh?=
PC12 MmjISpVv[3Srb36gZZN{[Xl? M{DvbWlvcGmkaYTpc44hd2ZibnXyeoUh\3Kxd4ToJIZi[3Sxcj3t[YRq[XSnZDDNRXAhc2mwYYPlJIFkfGm4aYT5JIlvKGi3bXHuJHBEOTJiY3XscJMtKEmFNUCgQUAzKM7:TT6= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB5N{O2N{c,OThyN{ezOlM9N2F-
HT-29 NYDZc2MzSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NHPMTGQ2KGSjeYO= MYnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWLUK5JINmdGy|IHHmeIVzKDViZHH5d{BjgSCZU2SxJIF{e2G7LDDJR|UxKD1iNDFOwG0v NVzreFIyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwO|g{OTZpPkK1NFc5OzF4PD;hQi=>
IEC6 MXrGeY5kfGmxbjDhd5NigQ>? Ml2wOUBucW6| M3K2[GlvcGmkaYTpc44hd2ZiTVXLNU8zKGmwIILheEBKTUN4IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBGWktzL{KgcI9weCCyaH;zdIhwenmuYYTpc44h\G:|ZXSgN|AhdWmwczDi[YZwemVidH:gd5RqdXWuYYTpc44hf2m2aDCxNEUhe2W{dX2g[o9zKDVibXnud{BjgSCrbX31co9jdG:2dHnu[{Bu\XSqb3SsJGlEPTBiPTC0MlIh|ryPLh?= NIjpdWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC3PFMyPid-MkWwO|g{OTZ:L3G+
NG 108-15 MnfuSpVv[3Srb36gZZN{[Xl? NYfkUmZ{S2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{Bi[m:uaYPoJG1CWEtiYXP0bZZqfHliaX6gcY92e2VibnX1do9jdGG|dH;tZUBidmRicnH0JIdtcW:vYTDofYJzcWRiTlegNVA5NTF3IHPlcIx{NCCDY4Tpeol1gSB;IEGwJO69VS5? NG\KN2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUWzO|M2PCd-MUW1N|c{PTR:L3G+
PC12 Ml64SpVv[3Srb36gZZN{[Xl? NVnqOYp6OTBidV2= M2fL[FUhcHK| M1:xd2FkfGm4YYTpc44hd2ZiToLmNk9CWkViaX6gdoF1KFCFMUKgZ4VtdHNiYYPz[ZN{\WRiYYOgTG8uOSCycn;0[YlvKGmwZIXjeIlwdiCjdDCxNEB2VSCjZoTldkA2KGi{czDwdoV1emWjdHXkJJdqfGhiSl7LJIlvcGmkaYTvdkBUWDZyMEGyOUBnd3JiMTDodkBj\W[xcnWgZ49ueG:3bnSgZYRlcXSrb36gZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| NVrQOJljRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzOFU3QDVpPkKxN|Q2Pjh3PD;hQi=>
PC12 MV\GeY5kfGmxbjDhd5NigQ>? NVny[INoOTBidV2= NFTxWmw2KGi{cx?= MUnBZ5RqfmG2aX;uJI9nKE6{ZkKvRXJGKGmwIILheEBRSzF{IHPlcIx{KGG|c3Xzd4VlKGG|IFjPMVEheHKxdHXpckBqdmS3Y4Tpc44h[XRiMUCgeW0h[W[2ZYKgOUBpenNicILleJJm[XSnZDD3bZRpKE2HS{KgbY5pcWKrdH;yJHUxOTJ4IH\vdkAyKGi{IHLl[o9z\SClb33wc5Vv\CCjZHTpeIlwdiCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> MoXPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|NEW2PFUoRjJzM{S1Olg2RC:jPh?=
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HeLa NV\USZdKTnWwY4Tpc44h[XO|YYm= MYGyNEB2VQ>? MmewN{BpenN? M2nXdWlvcGmkaYTpc44hd2ZiRWLLNU8zKHCqb4PwbI9zgWyjdHnvckBqdiCqdX3hckBJ\UyjIHPlcIx{KGG2IEKwJJVOKGGodHXyJFMhcHK|IHL5JHdme3Sncn6gZoxwfHSrbnegZY5idHm|aYO= Ml;FQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3N{C2NVUoRjJ|NUewOlE2RC:jPh?=
RD MWnxTHRUKGG|c3H5 MV;xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWkRiY3XscJM> MlnLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB1643 NGfLVpFyUFSVIHHzd4F6 M3q0UpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQkG2OFMh[2WubIO= NHTjO4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
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SK-N-MC NFzxXJRyUFSVIHHzd4F6 M1;PT5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzz Mn7ZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
TC32 MojLdWhVWyCjc4PhfS=> MmnNdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgWGM{OiClZXzsdy=> M4XXfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
MDA-MB-231 MkXuSpVv[3Srb36gZZN{[Xl? MlfYOVAhfU1? MlfhSI94dnKnZ4XsZZRqd25ib3[gUW1ROiCrbjDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGG2IEWwJJVOKGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> NG[3Roo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyOlIzPid-MkK5NlYzOjZ:L3G+
MDA-MB-231 MmLISpVv[3Srb36gZZN{[Xl? NY\PfY9QPTBidV2= M1\vO2Rwf26{ZXf1cIF1cW:wIH;mJG1OWDliaX6gbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCjdDC1NEB2VSCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> M2r3NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUK2NlI3Lz5{MkmyOlIzPjxxYU6=

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Methods Test Index PMID
Western blot
c-Jun / α-tubulin / p-ERK / ERK / p-AKT / AKT; 

PubMed: 17482134     


ERK regulates c-Jun levels in melanoma. The indicated melanoma cell lines were treated with 50μM PD98059 (PD) or 10μM LY294002 (LY) for 12h in the absence of serum. Protein samples were analyzed by Western blots using the indicated antibodies. α-Tubulin reveals equal loading.

p-JNK / JNK / Cyclin D1 ; 

PubMed: 17482134     


Inhibition of the MEK/ERK pathway affects c-Jun, P-JNK and cyclin D1 levels. The indicated melanoma cell lines were treated with 50μM PD98059 for 16h. Protein samples were analyzed by Western blots using the indicated antibodies. α-Tubulin reveals equal loading.

p-HER2 / HER2 ; 

PubMed: 24244023     


TE1cells were treated with wortmannin (Akt signal inhibitor), PD98059 (MAPK signal inhibitor), or lapatinib (EGFR and HER2 dual inhibitor) at the indicated doses or with DMSO as a negative control for 48 h. HER2-related signaling molecules were assessed by Western blot. 

MMP9 / XIAP / VEGF ; 

PubMed: 29695559     


SK-Hep1 cells were treated 10 μM PD98059 for 48 h.  Protein levels of MMP-9, XIAP, VEGF, cyclin-D1, and pERK were evaluated with western blotting assay.

17482134 24244023 29695559
体内試験

Treatment of mice 30 minutes before focal cerebral ischemia with PD98059 protects against damage, resulting in a decrease in infarct volume. [5] Pretreated with PD98059 (10 mg/kg per i.v. injection) 30 minutes before and then together with hourly cerulein injections for 3 hours significantly ameliorates cerulein-induced acute pancreatitis ipancreatitis on the basis of pancreatic wet weight and histology. [6] Administration of PD98059 (10 mg/kg) in mice 1 hour after carrageenan causes a reduction in all the parameters of inflammation measured. [7]

お薦めの試験操作（参考用のみ）

キナーゼ試験：

[1]
- 合併

In vitro MEK-inhibitory activity:

Incorporation of 32P into myelin basic protein (MBP) is assayed in the presence of glutathione S-transferase (GST) fusion proteins containing the 44-kDa MAPK (GST-MAPK) or the 45-kDa MEK (GST-MEK1). Assays are conducted in 50 μL of 50 mM Tris, pH 7.4/10 mM MgCl2/2 mM EGTA/10 μM [γ-32P]ATP containing 10 μg of GST-MEK1, 0.5 μg of GST-MAPK, and 40 μg of MBP. After incubation at 30°C for 15 minutes, reactions are stopped by addition of Laemmli SDS sample buffer. Phosphorylated MBP is resolved by SDS/10% PAGE.
細胞試験：

[1]
- 合併
  • 細胞株： K-Balb, KNRK, v-raf-3Y1, SRA/3Y1, EGFR/3T3, and K562
  • 濃度： Dissolved in DMSO, final concentrations ~100 μM
  • 反応時間： 3 dyas, or 7-10 days
  • 実験の流れ：

    For monolayer growth, cells are plated into multi-well plates at 10,000-20,000/mL. Forty-eight hours later, various concentrations of PD98059 are added to the cell growth medium and incubation is continued for an additional 3 days. Cells are then removed from the wells by incubation with trypsin and enumerated with a Coulter Counter. For growth in soft agar, cells are seeded into 35-mm dishes at 5,000-10,000 cells per dish with growth medium containing 0.3% agar and desired concentrations of PD98059. After 7-10 days of growth, visible colonies are manually enumerated with the aid of a dissecting microscope.


    (参考用のみ)
動物試験：

[7]
- 合併
  • 動物モデル： Male Sprague–Dawley rats with acute pancreatitis
  • 投薬量： 10 mg/kg
  • 投与方法： Injection i.v.
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 14 mg/mL (52.37 mM) warming
Water Insoluble
Ethanol Insoluble
体内 左から（NMPから）右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
4% DMSO+30% PEG 300+5% Tween 80+ddH2O
混合させたのち直ちに使用することを推奨します。 		1mg/mL

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。

化学情報

分子量 267.28
化学式

C16H13NO3
CAS No. 167869-21-8
Storage powder
in solvent
別名 N/A
Smiles COC1=CC=CC(=C1N)C2=CC(=O)C3=CC=CC=C3O2

投与溶媒組成計算器(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）
投与量 mg/kg 動物平均体重 g 投与体積（動物毎） ul 動物数 匹
ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）
% DMSO % % Tween 80 % ddH2O
計算リセット

便利ツール

モル濃度計算器

モル濃度計算器

求めたい質量、体積または濃度を計算してください。

質量 (mg) = 濃度 (mM) x 体積 (mL) x 分子量 (g/mol)

モル濃度計算器方程式

  • 質量
    濃度
    体積
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとSDS/COA（製品ページで利用可能な）。

希釈計算器

希釈計算器

貯蔵液を準備するために必要な希釈率を計算してください。Selleck希釈計算器は、以下の方程式に基づきます：

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます：C1V1 = C2V2 ( 入力 出力 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとSDS/COA（オンラインで利用できる）で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください：

総分子量：g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

質量 濃度 体積 分子量

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

  • * 必須

よくある質問（FAQ）

  • 質問1：

    How to formulate this inhibitor for i.p. injection?

  • 回答：

    You can prepare the stock by the vehicle 30% PEG400/0.5% Tween80/5% Propylene glycol, 0.5% CMC.

selleck catalog

MEKシグナル伝達経路

MEK Inhibitors with Unique Features

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  • U0126-EtOH

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    Features:A chemically synthesized and highly selective inhibitor of both MEK1 and MEK2.

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