Staurosporine (AM-2282)

For research use only. Not for use in humans.

製品コードS1421 別名:Antibiotic AM-2282, STS

Staurosporine (AM-2282)化学構造

CAS No. 62996-74-1

Staurosporine (AM-2282, Antibiotic AM-2282, STS) is a potent PKC inhibitor for PKCα, PKCγ and PKCη with IC50 of 2 nM, 5 nM and 4 nM, less potent to PKCδ (20 nM), PKCε (73 nM) and little active to PKCζ (1086 nM) in cell-free assays. Also shows inhibitory activities on other kinases, such as PKA, PKG, S6K, CaMKII, etc. Phase 3.

サイズ 価格(税別)  
JPY 46800
JPY 85000
JPY 216560
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バルク問合せ

文献中Selleckの製品使用例(96)

製品安全説明書

Antineoplastic and Immunosuppressive Antibiotics阻害剤の選択性比較

生物活性

製品説明 Staurosporine (AM-2282, Antibiotic AM-2282, STS) is a potent PKC inhibitor for PKCα, PKCγ and PKCη with IC50 of 2 nM, 5 nM and 4 nM, less potent to PKCδ (20 nM), PKCε (73 nM) and little active to PKCζ (1086 nM) in cell-free assays. Also shows inhibitory activities on other kinases, such as PKA, PKG, S6K, CaMKII, etc. Phase 3.
ターゲット
PKCα [1]
(Cell-free assay)
c-Fgr [2]
(Cell-free assay)
phosphorylase kinase [2]
(Cell-free assay)
PKCη [1]
(Cell-free assay)
PKCγ [1]
(Cell-free assay)
2 nM 2 nM 3 nM 4 nM 5 nM
体外試験

Staurosporine, a microbial alkaloid, significantly inhibits protein kinase C from rat brain with IC50 of 2.7 nM. Staurosporine displays strong inhibitory effect against HeLa S3 cells with IC50 of 4 nM. [1] Staurosporine also inhibits a variety of other protein kinases, including PKA, PKG, phosphorylase kinase, S6 kinase, Myosin light chain kinase (MLCK), CAM PKII, cdc2, v-Src, Lyn, c-Fgr, and Syk with IC50 of 15 nM, 18 nM, 3 nM, 5 nM, 21 nM, 20 nM, 9 nM, 6 nM, 20 nM, 2 nM, and 16 nM, respectively. [2] Staurosporine (1 μM) induces >90% apoptosis in PC12 cells. Consistently, Staurosporine treatment induces a rapid and prolonged elevation of intracellular free calcium levels [Ca2+]i, accumulation of mitochondrial reactive oxygen species (ROS), and subsequent mitochondrial dysfunction. [3] The apoptosis of MCF7 cells induced by Staurosporine can be enhanced by the expression of functional caspase-3 via caspase-8 activation and Bid cleavage. [4] Staurosporine treatment at 1 μM only partially inhibits IL-3-stimulated Bcl2 phosphorylation but completely blocks PKC-mediated Bcl2 phosphorylation. [5] Staurosporine induces apoptosis of human foreskin fibroblasts AG-1518, depending on the lysosomal cathepsins D mediated cytochrome c release and caspase activation. [6] In addition to activating the classical mitochondrial apoptosis pathway, Staurosporine triggers a novel intrinsic apoptosis pathway, relying on the activation of caspase-9 in the absence of Apaf-1. [7]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human colon carcinoma cell line HCT116 NIjUWYpHfW6ldHnvckBie3OjeR?= MmHBR49v[2WwdILheIlwdiC{ZYH1bZJm\CCob4Kg[5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gZ49td25iY3HyZ4lvd22jIHPlcIwhdGmwZTDIR3QyOTZuIFnDOVA:PiCwTT6= NWexOllVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW1N|c{PDVpPkG1OVM4OzR3PD;hQi=>
human PBMC NXnpOY9rTnWwY4Tpc44h[XO|YYm= MmjlNlQhcA>? NITuXFRUfXCycnXzd4lwdiCxZjDJUFIheHKxZIXjeIlwdiCrbjDoeY1idiCSQl3DJIFnfGW{IEK0JIhzeyCkeTDFUGlUSSxiSVO1NF0yPiCwTT6= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDV6NUC0Okc,OTh3OEWwOFY9N2F-
endothelial cells MW\GeY5kfGmxbjDhd5NigQ>? M4rtOmVn\mWldHn2[UBld3OnIHHnZYlve3RicHzhd41qdm:pZX6gZYN1cX[jdH;yJIFkfGm4aYT5JJN1cW23bHH0[YQh[nlicHjvdoJwdCCnc4TldkBqdiCnbnTveIhmdGmjbDDj[YxteyxiRVS1NEA:KDdwNTFOwG0v NYrjUplFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOEewPVA6PSd-OEewPVA6PTxxYU6=
Sf9 insect cells MlrWSpVv[3Srb36gZZN{[Xl? M4LJcmlvcGmkaYTpc44hd2ZicILveIVqdiCtaX7hd4UhSyhzOkHtbZh1fXKnIH;mJHBMSyCjbIDoZUAnKGKndHGpJIV5eHKnc4Pl[EBqdiCVZkmgbY5{\WO2IHPlcIx{NCCLQ{WwJF0hOC5yMjFOwG0v M4fuVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl6N{O2NFUoRjl6N{O2NFU9N2F-
LoVo cells NIjtbZlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= Mn7sRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiClb3zvckBk[W6lZYKgZ4VtdCCuaX7lJEhNd1[xIHPlcIx{MSC3c3nu[{BOXFRiYYPzZZktKEmFNUCgQUAxNjByMTFOwG0v NYjZO3hKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG1PVE2ODVpPkGxOVkyPTB3PD;hQi=>
umbilical vein endothelial cells M1PIR2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MX\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKHWvYnnsbYNidCC4ZXnuJIVv\G:2aHXsbYFtKGOnbHzzJEhJXV[HQ4OpJJV{cW6pIF3UWEBie3OjeTygTWM2OCB;IECuNFA1KM7:TT6= MnzhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF3OUG1NFUoRjFzNUmxOVA2RC:jPh?=
ST-486 cells NV;YXWpSSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NEPxS4xCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIJ2emurdDDsfY1xcG:vYTDj[YxtKGyrbnWgLHNVNTR6NjDj[YxteylidYPpcochVVSWIHHzd4F6NCCLQ{WwJF0hOC5yMEeg{txONg>? MkXHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF3OUG1NFUoRjFzNUmxOVA2RC:jPh?=
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HEK293 cells MkG0SpVv[3Srb36gZZN{[Xl? NE\GfYRKdmirYnn0bY9vKG:oIGDLR{1j\XSjIFnJJI1m\GmjdHXkJGlNNThicnXs[YF{\SCocn;tJGhGUzJ7MzDj[YxteyCkeTDFUGlUSSxiSVO1NEA:KDBwMEe3JO69VS5? M{PxRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{OUSxN|MyLz5zMkm0NVM{OTxxYU6=
P19 cells NEnodnNHfW6ldHnvckBie3OjeR?= NHrRXHlKdmirYnn0bY9vKG:oIGDsZZRmdGW2LXTldol3\WRiZ4Lve5RpKG[jY4TvdkBz\WOncITvdkBqdiCSMUmgZ4VtdHNuIFnDOVAhRSByLkCwNkDPxE1w MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd5MUSxPUc,OTV5N{G0NVk9N2F-
P19 cells NWrrOVd7TnWwY4Tpc44h[XO|YYm= M4DB[2lvcGmkaYTpc44hd2ZiUHzheIVt\XRvZHXybZZm\CCpcn;3eIgh\mGldH;yJJJm[2WydH;yJIlvKFBzOTDj[YxteyxiSVO1NEA:KDBwMECyJO69VS5? MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd5MUSxPUc,OTV5N{G0NVk9N2F-
P19 cells NET4WGlHfW6ldHnvckBie3OjeR?= NEnsTZhKdmirYnn0bY9vKG:oIGDyc5RmcW5iS3nuZZNmKEFiaX6gVFE6KGOnbHzzMEBKSzVyIE2gNE4xODRizszNMi=> NIPBelc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe3NVQyQSd-MUW3O|E1OTl:L3G+
P19 cells M1q1b2Z2dmO2aX;uJIF{e2G7 Mn;rTY5pcWKrdHnvckBw\iCFYXzjbZVuN2OjbH3v[JVtcW5vZHXw[Y5l\W62IIDyc5RmcW5ia3nuZZNmKHS7cHWgTWkhcW5iUEG5JINmdGy|LDDJR|UxKD1iMD6wNFYh|ryPLh?= NHXkNlY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe3NVQyQSd-MUW3O|E1OTl:L3G+
P19 cells M2XRNWZ2dmO2aX;uJIF{e2G7 NV:3bGxoUW6qaXLpeIlwdiCxZjDDfYNtcW5vZHXw[Y5l\W62IHvpcoF{\SBzIHnuJHAyQSClZXzsd{whUUN3MDC9JFAvODB6IN88UU4> M4\lbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{exOFE6Lz5zNUe3NVQyQTxxYU6=
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HEK293 cells MUfGeY5kfGmxbjDhd5NigQ>? MnTTTY5pcWKrdHnvckBw\iCLTD24JJJmdGWjc3WgZpkhUEWNMkmzJINmdGy|IHX4dJJme3OrbnegVGtENWKndHGyMEBKSzVyIE2gNE4xPzdizszNMi=> Ml;XQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5N{G0NVkoRjF3N{exOFE6RC:jPh?=
P19 cells NE\JOGhHfW6ldHnvckBie3OjeR?= MUHJcohq[mm2aX;uJI9nKEmwc4XsbY4hemWlZYD0c5Ihc2mwYYPlJIlvKFBzOTDj[YxteyxiSVO1NEA:KDBwMjFOwG0v MlrDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5N{G0NVkoRjF3N{exOFE6RC:jPh?=
P19 cells NGnoUG9HfW6ldHnvckBie3OjeR?= M1jv[mlvcGmkaYTpc44hd2ZiQ3Hz[YlvKGurbnHz[UAyKGmwIGCxPUBk\WyuczygTWM2OCB;IEGuOEDPxE1w NIDycJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe3NVQyQSd-MUW3O|E1OTl:L3G+
FL5.12-Akt1 cells M1vJO2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NHTLO2ZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGZNPS5zMj3Bb5QyKGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwMkmg{txONg>? MmXrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MEO2NlYoRjF4NECzOlI3RC:jPh?=
MiaPaCa-2 cells M2qycmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MlvBRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDNbYFR[UOjLUKgZ4VtdHNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4{PyEQvF2u MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjRyM{[yOkc,OTZ2MEO2NlY9N2F-
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A549 cells MV;DfZRwfG:6aXPpeJkh[XO|YYm= NUiwPHF6PzJiaB?= NX7wN3NjS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBu\XSqb3SsJGdKPTBiPTCwMlAxOjRizszNMi=> MnHvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh2OES3O|UoRjF6NEi0O|c2RC:jPh?=
MDA-MB-231 cells M3:4XGN6fG:2b4jpZ4l1gSCjc4PhfS=> MVm3NkBp NIfOWpNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIH3leIhw\CxiR1m1NEA:KDBwMEC3NUDPxE1w Mn34QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh2OES3O|UoRjF6NEi0O|c2RC:jPh?=
HT29 cells NIjTcIREgXSxdH;4bYNqfHliYYPzZZk> MU[3NkBp MVfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIWFI6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIH3leIhw\CxiR1m1NEA:KDBwMEGwPUDPxE1w MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDR6NEe3OUc,OTh2OES3O|U9N2F-
MCF7 cells NFXWeYNHfW6ldHnvckBie3OjeR?= MWjJcohq[mm2aX;uJI9nKFCNQTDpckBpfW2jbjDNR2Y4KGOnbHzzJIJ6KGG{cnH5MYJie2WmIH\seY9z\XOlZX7j[UBie3OjeTygT4khRSByLkCwOUDPxE1w NFPPb2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[1OlM3QSd-MUi2OVY{Pjl:L3G+
MCF7 cells NYexVpJCTnWwY4Tpc44h[XO|YYm= NWTZSJp[UW6qaXLpeIlwdiCxZjDQT2EhcW5iaIXtZY4hVUOINzDj[YxteyCkeTDhdpJigS2kYYPl[EBndHWxcnXzZ4Vv[2ViYYPzZZktKEmFNUCgQUAzKM7:TT6= NIPLSXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[1OlM3QSd-MUi2OVY{Pjl:L3G+
Sf9 cells NWj4[HVtTnWwY4Tpc44h[XO|YYm= MVLJcohq[mm2aX;uJI9nKGi3bXHuJHN6cyCneIDy[ZN{\WRiaX6gV4Y6KGOnbHzzMEBKSzVyIE2gNE4xODNizszNMi=> MmXKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh6MkO3PFQoRjF6OEKzO|g1RC:jPh?=
Sf9 cells NHL3fpdHfW6ldHnvckBie3OjeR?= MV\Jcohq[mm2aX;uJI9nKGi3bXHuJHpCWDdyIHX4dJJme3OnZDDpckBU\jliY3XscJMtKEmFNUCgQUAxNjB3MzFOwG0v NEHHU2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEiyN|c5PCd-MUi4NlM4QDR:L3G+
insect cells NInHV2NHfW6ldHnvckBie3OjeR?= NH3p[HlKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IGDpcVEh\XiycnXzd4VlKGmwIHnud4VkfCClZXzsd{BjgSCKVGLGMEBKSzVyIE2gNE4xOSEQvF2u MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTF5OUC3Okc,OTlzN{mwO|Y9N2F-
HT-29 cells MXfBdI9xfG:|aYOgZZN{[Xl? MUWyJIg> NX3rN2dvUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDIWE0zQSClZXzsd{Bie3Onc4Pl[EBieyClaHHu[4UhcW5ibXn0c4Npd26mcnnhcEB1emGwc33lcYJz[W6nIIDveIVvfGmjbDDh[pRmeiB{IHjyd{BjgSCMQ{Ggd5RicW6rbnegZoF{\WRiZnz1c5Jme2OnbnPlJIF{e2G7LDDFSFUxKD1iMD6wNFI3KM7:TT6= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR{MkKwOkc,OTl2MkKyNFY9N2F-
insect Sf21 cells M4nBZ2Z2dmO2aX;uJIF{e2G7 M2rQWWlvcGmkaYTpc44hd2ZiSlHLN{BmgHC{ZYPz[YQhcW5iaX7z[YN1KFOoMkGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCkaX;0bY56dGG2ZXSgd5Vje3S{YYTlJJBpd3OyaH;yfYxifGmxbjygTWM2OCB;IECuNFA3KM7:TT6= M{ntVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEK3NlA{Lz5zOUSyO|IxOzxxYU6=
Jurkat cells NEH3[XVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NHTRSmdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGpCUzNiZYjwdoV{e2mwZzDJUFIue3SrbYXsZZRm\CCqdX3hckBLfXKtYYSgZ4VtdHNuIFnDOVAhRSByLkC3NUDPxE1w M3;UO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEK3NlA{Lz5zOUSyO|IxOzxxYU6=
Jurkat cells NITYUVJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NH\iSJNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGpCUzNiZYjwdoV{e2mwZzDoeY1idiC{ZYP0bY5oKEq3cnvheEBk\WyuczygTWM2OCB;IECuN|EyKM7:TT6= NILCZ5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUSyO|IxOyd-MUm0NlczODN:L3G+
HUE cells NWe1dpZmTnWwY4Tpc44h[XO|YYm= M2jwOlkxKG2rboO= MlT0TY5pcWKrdHnvckBw\iCYRVfGVlIhcW5iSGXFJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiVlXHSk1qdmS3Y3XkJIF2fG:yaH;zdIhwenmuYYTpc44hfHKnYYTl[EBnd3JiOUCgcYlveyCkZX\vdoUhXkWJRjDjbIFtdGWwZ3WgZpkhTUyLU1GsJGlEPTBiPTCwMlA4KM7:TT6= NGjueYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG3NFE3Oyd-MkCxO|AyPjN:L3G+
Sf9 cells MoHtSpVv[3Srb36gZZN{[Xl? NYDBVlR7PjBibXnudy=> NVjSOoxrUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDHV3Qu\nW|ZXSgUmlMKGW6cILld5Nm\CCrbjDT[lkh[2WubIOgZYZ1\XJiNkCgcYlveyCkeTDyZYRqd22ndILpZ{Bxem:2ZXnuJItqdmG|ZTDhd5NigSxiSVO1NEA:KDJwMzFOwG0v Mny1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB3OEC1OVIoRjJyNUiwOVUzRC:jPh?=
Sf21 cells NWr6[I5wTnWwY4Tpc44h[XO|YYm= MlT2N|AhfU1? NX\LSGZYUW6qaXLpeIlwdiCxZjDoeY1idiCFSFuyJItqdmG|ZTDlfJBz\XO|ZXSgbY4hcW6|ZXP0JHNnOjFiY3XscJMhfXOrbnegdIhwe3Cqbz3DVmVDfGmmZTDhd{B{fWK|dILheIUh[XRiM{CgeW0tKEmFNUCgQUAxNjB{NzFOwG0v MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN|NEe5Okc,OjF|M{S3PVY9N2F-
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HeLa cells MWPDfZRwfG:6aXPpeJkh[XO|YYm= MkK3OFghcA>? MoTnR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4xODByMESg{txONg>? NUfrNJJvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzPFgyQTFpPkKxN|g5OTlzPD;hQi=>
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HT-29 cells M1jWWGZ2dmO2aX;uJIF{e2G7 NUjLWpV7OiCq M33wOmVn\mWldDDvckBucXSxY3jvcoRzcWGuIH3lcYJz[W6nIIDveIVvfGmjbDDpckBpfW2jbjDIWE0zQSClZXzsd{Bi\nSncjCyJIhzeyC3c3nu[{BLSy1zIIP0ZYlvcW6pIHL5JIZtfW:{ZYPj[Y5k\SCjc4PhfUwhUUN3MDC9JFAvODB{IN88UU4> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR{OEO3OUc,OjF2MkizO|U9N2F-
HeLa cells NV7j[G5PS3m2b4TvfIlkcXS7IHHzd4F6 NWfK[HlpPDhiaB?= M4jsdmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmVGFiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODByMEC0JO69VS5? NHXpWIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS4PFY2PSd-MkG0PFg3PTV:L3G+
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HT-29 cells M1nydWZ2dmO2aX;uJIF{e2G7 NV36N|Y5UW6qaXLpeIlwdiCxZjDtbZRw[2ixbnTybYFtKG2nbXLyZY5mKHCxdHXueIlidCCrbjDoeY1idiCKVD2yPUBk\WyuczD1d4lv\yCMQ{Gg[JlmKHO2YXnubY5oKGK7IH\seY9z\XOlZX7j[UBxdGG2ZTDy[YFl\XJiYYPzZZktKEmFNUCgQUAxNjByMkWg{txONg>? NUT1fmtlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzQTNpPkKxOVE{Ojl|PD;hQi=>
SF268 cells NFP0SXhEgXSxdH;4bYNqfHliYYPzZZk> MmHxOFghcA>? Moj2R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2YzPjhiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhT0l3MDC9JFAvODR2IN88UU4> NGLQUHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxN|I6PCd-MkG1NVMzQTR:L3G+
CHOK1 cells NVL0VpZuS3m2b4TvfIlkcXS7IHHzd4F6 NGr6dXg1QCCq Moe0R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR2hQUzFiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhT0l3MDC9JFAvOTNizszNMi=> M1vyZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGzNlk1Lz5{MUWxN|I6PDxxYU6=
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HT-29 cells Mke3R5l1d3SxeHnjbZR6KGG|c3H5 NIewbm81QCCq MUnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIWE0zQSClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBIUTVyIE2gN{43KM7:TT6= NVzqV2I3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzQTRpPkKxOVE{Ojl2PD;hQi=>
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LoVo cells NH\YSY1CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M4flVFQ5KHSxIEeyJIg> MoXxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCOb2\vJINmdGy|IHHmeIVzKDR6IITvJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjByMTFOwG0v MnvpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzOEK5NlkoRjJ{MUiyPVI6RC:jPh?=
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MCF7 cells NX3tdmdRSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NUH6R5VxPDhiaB?= NX3zNXM3SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCzMlA4KM7:TT6= NXyySWFVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1NFM{PjRpPkKyOVA{OzZ2PD;hQi=>
A431 cells M2[2d2N6fG:2b4jpZ4l1gSCjc4PhfS=> M4\uVFI1KGh? NGfUbZVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPDNzIHPlcIx{KGGodHXyJFI1KGi{czD1d4lv\yCDbn7lfIlvKF[nRlnUR{9xem:yaXTpeY0hcW:maXTlJJN1[WmwaX7nJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwMEeg{txONg>? MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV2MUC1NUc,OjJ3NEGwOVE9N2F-
MCF7 cells NWDYWpplS3m2b4TvfIlkcXS7IHHzd4F6 NUmxSGV[OjRiaB?= NFzNU3NEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFI1KGi{czD1d4lv\yCDbn7lfIlvKF[nRlnUR{9xem:yaXTpeY0hcW:maXTlJJN1[WmwaX7nJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwMUig{txONg>? NGf6S2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW0NVA2OSd-MkK1OFExPTF:L3G+
BJ cells NHXz[WpEgXSxdH;4bYNqfHliYYPzZZk> MUG3NkBp NUK0W4dVS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSkpiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGNidGOnaX6gRW0h[XO|YYmsJGlEPTBiPTCwMlAxOiEQvF2u NITsbIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyNVA5OSd-MkK5NlExQDF:L3G+
CEM cells MkHUR5l1d3SxeHnjbZR6KGG|c3H5 NIfPd4k4OiCq MYrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDDSW0h[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IFPhcINmcW5iQV2gZZN{[XluIFnDOVAhRSByLkCyN{DPxE1w NVXmOYR7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5NlExQDFpPkKyPVIyODhzPD;hQi=>
MCF7 cells MUnDfZRwfG:6aXPpeJkh[XO|YYm= NHv2Wlc4OiCq NX;he3M3S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA4OiCqcoOgZpkhS2GuY3XpckBCVSCjc4PhfUwhUUN3MDC9JFAvODZ2IN88UU4> NYPCd4JnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5NlExQDFpPkKyPVIyODhzPD;hQi=>
HeLa cells M3jiTGN6fG:2b4jpZ4l1gSCjc4PhfS=> M33S[lczKGh? MmXMR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bi\nSncjC3NkBpenNiYomgR4Ft[2WrbjDBUUBie3OjeTygTWM2OCB;IECuNVc2KM7:TT6= NEG2N|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyNVA5OSd-MkK5NlExQDF:L3G+
HepG2 cells NFnYTmFHfW6ldHnvckBie3OjeR?= NF;LOVMzPCCq NV7aZ4Z7UW6qaXLpeIlwdiCxZjD0[YxwdWW{YYPlJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFI1KGi{czDifUBVWkGSLWDDVk1GVEmVQTDhd5NigSxiSVO1NEA:KDRwMUSg{txONg>? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzBzOEC5Okc,OjNyMUiwPVY9N2F-
SW1116 cells M4LhWmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NFrnNXQ1QCCq Mn;TRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVV{GxNVYh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBu\XSqb3SsJGlEPTBiPTC0Mlk2KM7:TT6= NUCyO2lvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNVgxQTZpPkKzNFE5ODl4PD;hQi=>
HepG2 cells NGLWcXdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MlPjOFghcA>? NVnkZZhjSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKG2ndHjv[EwhUUN3MDC9JFYvPzNizszNMi=> M1n3eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEG4NFk3Lz5{M{CxPFA6PjxxYU6=
BGC823 cells NFfjdmJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NF6wWZg1QCCq M{nCZmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQlfDPFI{KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQhdWW2aH;kMEBKSzVyIE2gOk45OyEQvF2u M{LDcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEG4NFk3Lz5{M{CxPFA6PjxxYU6=
HeLa cells MV7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NIHTbJc1QCCq MW\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEinTHGgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDt[ZRpd2RuIFnDOVAhRSB7LkGyJO69VS5? MonOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMUiwPVYoRjJ|MEG4NFk3RC:jPh?=
HCT116 cells MVzGeY5kfGmxbjDhd5NigQ>? NETCbmNKdmirYnn0bY9vKG:oIGTOTWsudWWmaXH0[YQhXEOIND;i[ZRiNWOjc3XpckB1emGwc3PybZB1cW:wIHnuJIh2dWGwIFjDWFEyPiClZXzsd{BjgSCkZYThMYxi[3SjbXHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwJF0hOC5yM{mg{txONg>? NIPqWVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{KzNlA3OCd-MkOyN|IxPjB:L3G+
HepG2 cells MVjGeY5kfGmxbjDhd5NigQ>? MoLKOFghcA>? NVXvVZgxUW6qaXLpeIlwdiCxZjD0[YxwdWW{YYPlJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFQ5KGi{czDifUBVWkGSLWDDVk1GVEmVQTygTWM2OCB;IEiuN{DPxE1w MoHMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{N{m4OlQoRjJ|Mke5PFY1RC:jPh?=
SW1116 cells MV7GeY5kfGmxbjDhd5NigQ>? Mn;xNlQhcA>? MU\Jcohq[mm2aX;uJI9nKHSnbH;t[ZJie2ViaX6gbJVu[W5iU2exNVE3KGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDUVmFRNVCFUj3FUGlUSSCjc4PhfUwhUUN3MDC9JFQvOThizszNMi=> M2j6ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mki2O|YyLz5{NEK4Olc3OTxxYU6=
KE-97 cells M3;3[WN6fG:2b4jpZ4l1gSCjc4PhfS=> MkTIO|IhcA>? NXSwRXBJS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0VvOUegZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRz\S2JbH:gcJVucW6nc3PlcpQh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVAhRSByLkGzJO69VS5? M4DNO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{K4Nlg{Lz5{NEOyPFI5OzxxYU6=
Jurkat cells NH7oeIJEgXSxdH;4bYNqfHliYYPzZZk> M{OzdVczKGh? MVTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDKeZJs[XRiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGNmdGyWaYTy[U1IdG9ibIXtbY5me2OnboSgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAxNjF2IN88UU4> Ml62QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MkiyPFMoRjJ2M{K4Nlg{RC:jPh?=
HuH7 cells NYDOWJBxS3m2b4TvfIlkcXS7IHHzd4F6 NYDKc3FUPzJiaB?= NUDo[W1VS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUHWKNzDj[YxteyCjZoTldkA4OiCqcoOgZpkhS2WubGTpeJJmNUeubzDseY1qdmW|Y3XueEBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDBwMkOg{txONg>? NVrpcJBRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNlgzQDNpPkK0N|I5Ojh|PD;hQi=>
BGC823 cells NVLie3gxS3m2b4TvfIlkcXS7IHHzd4F6 NYHPOVdHPzJiaB?= M17C[mN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGJISzh{MzDj[YxteyCjZoTldkA4OiCqcoOgZpkhS2WubGTpeJJmNUeubzDseY1qdmW|Y3XueEBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDBwM{ig{txONg>? NGfQVYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOyPFI5Oyd-MkSzNlgzQDN:L3G+
MCF7 cells M{L5OWN6fG:2b4jpZ4l1gSCjc4PhfS=> NFv3fGE4OiCq MX7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDD[YxtXGm2cnWtS4xwKGy3bXnu[ZNk\W62IHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyIE2gNE42OiEQvF2u MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN{OEK4N{c,OjR|MkiyPFM9N2F-
NIH/3T3 cells MV3DfZRwfG:6aXPpeJkh[XO|YYm= NXXXRXZFQTZiaB?= Mk[2R5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUmlJNzOWMzDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwJF0hOC5{IN88UU4> MmDFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NkG1NlEoRjJ2M{[xOVIyRC:jPh?=
A2780 cells NF;5[VFEgXSxdH;4bYNqfHliYYPzZZk> NX7sWplmQTZiaB?= NWHjb5RIS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTJ5OECgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KFOUQjDhd5NigSxiSVO1NEA:KDBwMjFOwG0v NGfGUGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2NVUzOSd-MkSzOlE2OjF:L3G+
HT-29 cells NGe0ZXlEgXSxdH;4bYNqfHliYYPzZZk> NHXG[o46PiCq NHTKR4dEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJXC1{OTDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwJF0hOC5{IN88UU4> NVvBeVRJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlE2OjFpPkK0N|YyPTJzPD;hQi=>
8505C cells MXzDfZRwfG:6aXPpeJkh[XO|YYm= NF;ER|Y6PiCq NYLOcGl6S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hQDVyNVOgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KFOUQjDhd5NigSxiSVO1NEA:KDBwMjFOwG0v NHH3Oo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2NVUzOSd-MkSzOlE2OjF:L3G+
518A2 cells M4DyNmN6fG:2b4jpZ4l1gSCjc4PhfS=> MmPCPVYhcA>? M1jBXGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJFUyQEF{IHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEmFNUCgQUAxNjJizszNMi=> MmLqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NkG1NlEoRjJ2M{[xOVIyRC:jPh?=
MCF7 cells NGjYW2REgXSxdH;4bYNqfHliYYPzZZk> M1Gyflk3KGh? MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTBiPTCwMlQh|ryPLh?= NXzpT4xPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlE2OjFpPkK0N|YyPTJzPD;hQi=>
A549 cells NIezU5BEgXSxdH;4bYNqfHliYYPzZZk> MVO5OkBp MUPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTBiPTCwMlYh|ryPLh?= NIjOS3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2NVUzOSd-MkSzOlE2OjF:L3G+
V79 MZ cells M17x[2Z2dmO2aX;uJIF{e2G7 MX3Jcohq[mm2aX;uJI9nKGi3bXHuJIFt\G:|dHXyc45mKHO7boToZZNmKGW6cILld5Nm\CCrbjDWO|khVVpiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBidGSxc4Tldo9v\SC|eX70bIV{cXNuIFnDOVAhRSByLkCxNUDPxE1w NV;sUW4{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0NlI2OTlpPkK0OFIzPTF7PD;hQi=>
HEK293 cells MlTvR5l1d3SxeHnjbZR6KGG|c3H5 NVPUemNEPzJiaB?= Mo\LR5l1d3SxeHnjbZR6KGGpYXnud5QhUEWNMkmzJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XKJbH:gZZN{[XluIFnDOVAhRSByLkC1OkDPxE1w NWf1V3g5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OlMzPjJpPkK0O|Y{OjZ{PD;hQi=>
HeLa cells NFG4NXhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M{f4T|I1KHSxIES4JIg> NUHyZpZ2SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IEK0JJRwKDR6IHjyd{BjgSCFQ1utPEBie3OjeTygTWM2OCB;IEKuO|Ih|ryPLh?= NIPzOmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe5NlgyOSd-MkS3PVI5OTF:L3G+
A549 cells MnzWRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NXTROYE1OjRidH:gOFghcA>? M{XVO2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDh[pRmeiB{NDD0c{A1QCCqcoOgZpkhS0ONLUigZZN{[XluIFnDOVAhRSB|LkC1JO69VS5? M1TENFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{myPFEyLz5{NEe5NlgyOTxxYU6=
HepG2 cells M4TKVGN6fG:2b4jpZ4l1gSCjc4PhfS=> NGDpZ|VEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\XCJMjDj[YxteyxiRVO1NEA:KDJizszNMi=> NX;LUnJFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNVY{OTdpPkK1N|E3OzF5PD;hQi=>
Raji cells M{fqemN6fG:2b4jpZ4l1gSCjc4PhfS=> MV3DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDSZYpqKGOnbHzzMEBGSzVyIE2gNkDPxE1w NUHJPXNGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNVY{OTdpPkK1N|E3OzF5PD;hQi=>
BJ cells NVv3[FlqS3m2b4TvfIlkcXS7IHHzd4F6 MYrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDCTkBk\WyuczygSWM2OCB;IEKg{txONg>? MkGzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MU[zNVcoRjJ3M{G2N|E4RC:jPh?=
HEK293 cells MXLDfZRwfG:6aXPpeJkh[XO|YYm= NVv4dm1sS3m2b4TvfIlkcXS7IHHnZYlve3RiSFXLNlk{KGOnbHzzMEBGSzVyIE2gNkDPxE1w M1frUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{G2N|E4Lz5{NUOxOlMyPzxxYU6=
A549 cells M3G5N2N6fG:2b4jpZ4l1gSCjc4PhfS=> MmGxOFghcA>? NXPRS25xS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5yMjFOwG0v NGPHXW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUiyOVk{PCd-MkW4NlU6OzR:L3G+
HeLa cells MV;DfZRwfG:6aXPpeJkh[XO|YYm= NXXjdFF6PDhiaB?= M3j1O2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmVGFiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODJ3IN88UU4> MkDuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6MkW5N|QoRjJ3OEK1PVM1RC:jPh?=
PC3 cells NHX2VGpEgXSxdH;4bYNqfHliYYPzZZk> MYe0PEBp NVvvS|UyS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWEN|IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjB|MTFOwG0v MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh{NUmzOEc,OjV6MkW5N|Q9N2F-
MGC-803 cells M4TRbWZ2dmO2aX;uJIF{e2G7 Mlq5NlQhcA>? MVHJcohq[mm2aX;uJI9nKHSnbH;t[ZJie2ViaX6gbJVu[W5iTVfDMVgxOyClZXzsd{Bi\nSncjCyOEBpenNiYomgWHJCWC2SQ2KtSWxKW0FiYYPzZZktKEmFNUCgQUA5Njl5IN88UU4> M2nmNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUCwOlU3Lz5{NkmwNFY2PjxxYU6=
Sf9 insect cells NH;FbVNHfW6ldHnvckBie3OjeR?= M2XqSFExKG2rboO= NUXtOpZVUW6qaXLpeIlwdiCxZjDOMZRmem2rbnHsJGdUXCC2YXfn[YQhemWlb33ibY5idnRiZoXscE1t\W6pdHigbJVu[W5iQ1jLNUBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOgV4Y6KGmwc3XjeEBk\WyuczDh[pRmeiBzMDDtbY5{KGK7IHz1cYlv\XOlZX70JGFFWC2JbH:gZZN{[XluIFnDOVAhRSByLkCwNVIh|ryPLh?= Ml65QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyOEmyNVEoRjJ5MEi5NlEyRC:jPh?=
Sf21 cells NXXBfZRvTnWwY4Tpc44h[XO|YYm= Mn[yOlAhdWmwcx?= NGf5dmRKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFrBT|Ih\XiycnXzd4VlKGmwIGPmNlEh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIGXsbYdpfC2FQVfBS2FKTVSGS1XZXXRXU0RicHjvd5Bpd3K7bHH0bY9vKHC{ZT3pcoN2[mG2ZXSgZoVnd3KnIIP1ZpN1emG2ZTDh[IRqfGmxbjDhcoQhdWWjc4Xy[YQh[W[2ZYKgOlAhdWmwczDifUBNSU6FRTDk[ZRm[3Srb36gcYV1cG:mLDDJR|UxKD1iMD6wNFIh|ryPLh?= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF|N{O1PUc,OjdzM{ezOVk9N2F-
Neuro2a cells MX3DfZRwfG:6aXPpeJkh[XO|YYm= Mme2O|IhcA>? MmjER5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUoV2em9{YTDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5yMUGzJO69VS5? M3m1TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUWyOFI4Lz5{OEG1NlQzPzxxYU6=
MDA-MB-231 cells M4P6WGZ2dmO2aX;uJIF{e2G7 NFnaRlQyPiCq M3K2[mlvcGmkaYTpc44hd2ZicnX0bY5w[myjc4TvcYEheHKxdHXpckBxcG:|cHjvdplt[XSrb36gbY4hcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDh[pRmeiBzNjDodpMh[nliSH;lZ4h{fCB|M{O0NkB{fGGrbnnu[{Bj[XOnZDDmcJVwemW|Y3XuZ4Uh[XO|YYmsJGlEPTBiPTCwMlA3PiEQvF2u M{\xdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEOxN|QzLz5{OESzNVM1OjxxYU6=
Sf9 insect cells MorsSpVv[3Srb36gZZN{[Xl? MoTwNlAhdWmwcx?= M{nGV2lvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iTj30[ZJucW6jbHz5JGdUXC22YXfn[YQhTUeIUjDMPFU5Wi:WN{mwUU9EPzl5UzD0dolxdGVibYX0ZY51KGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{BqdiCVZkmgbY5{\WO2IHPlcIx{KHC{ZXnuZ5Vj[XSnZDDmc5IhOjBibXnud{Bnd2yub4fl[EBjgSCjZHTpeIlwdiCxZjDbN|NRZS2DVGCgcYVie3W{ZXSgZYZ1\XJiMjDodpMh[nliZnnseIVzNWKrbnTpcochdWW2aH;kMEBKSzVyIE2gNE4xODB{OTFOwG0v M1PIOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEiyNVUyLz5{OES4NlE2OTxxYU6=
HEK293 cells M3XSRWN6fG:2b4jpZ4l1gSCjc4PhfS=> NXLHU5ZtPzJiaB?= M4jycmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEiHS{K5N{Bk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiClZXzsJJZq[WKrbHn0fUBi\nSncjC3NkBpenNiYomgR4VtdFSrdHXyJGdtdyCudX3pcoV{[2WwdDDhd5NigSxiQ1O1NEA:KDBwMECzOVQh|ryPLh?= M2jaeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NkK0O|AyLz5{OE[yOFcxOTxxYU6=
BT549 cells M3vpcWN6fG:2b4jpZ4l1gSCjc4PhfS=> NEjtXm8zPCCq NWr2bJBPS3m2b4TvfIlkKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gRnQ2PDliY3XscJMhcW6ldXLheIVlKG[xcjCyOEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4xQCEQvF2u M4rJPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6N{C1OFMzLz5{OEewOVQ{OjxxYU6=
MDA-MB-468 cells NX;sS|FVS3m2b4TvfIlkcXS7IHHzd4F6 NWW3[G9[OjRiaB?= NWnTV4JsS3m2b4TvfIlkKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUS2PEBk\WyuczDpcoN2[mG2ZXSg[o9zKDJ2IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkG1JO69VS5? MkfDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5MEW0N|IoRjJ6N{C1OFMzRC:jPh?=
MDA-MB-231 cells NFPsZlJEgXSxdH;4bYNqfHliYYPzZZk> NX3TTYhXOjRiaB?= M4DZemN6fG:2b4jpZ{Bi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiaX7jeYJifGWmIH\vdkAzPCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5{NDFOwG0v MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDdyNUSzNkc,Ojh5MEW0N|I9N2F-
Vero cells MV\DfZRwfG:6aXPpeJkh[XO|YYm= MVSyJIRigXN? MYfDfZRwfG:6aXPpeJkh[WejaX7zeEBC\nKrY3HuJIdz\WWwIH3vcotmgSCYZYLvJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJZq[WKrbHn0fUBi\nSncjCyJIRigXNiYomgZ4VtdC22aYTldk1odG9ibIXtbY5me2OnboSgZZN{[XluIGTDOVAhRSByLkCwNlQh|ryPLh?= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDd4M{[0OUc,Ojh5NkO2OFU9N2F-
L929 cells NV3TUZJrSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NVnTc|lXPSCmYYnz NXjxO|loSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W|ZTDMPVI6KGOnbHzzJIFnfGW{IEWg[IF6eyCkeTDXV3QuOSCjc4PhfUwhUUN3MDC9JFAvOiEQvF2u Ml3sQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NU[5NVUoRjJ6OUW2PVE2RC:jPh?=
KB-3-1 cells M2DDOGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NYXvbXFPPSCmYYnz MVXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEuELUOtNUBk\WyuczDh[pRmeiB3IHThfZMh[nliV2PUMVEh[XO|YYmsJGlEPTBiPTCwMlIh|ryPLh?= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl3NkmxOUc,Ojh7NU[5NVU9N2F-
MCF7 cells M4Hme2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NYrh[nlrPSCmYYnz NYPu[5RYSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEWg[IF6eyCkeTDXV3QuOSCjc4PhfUwhUUN3MDC9JFEvQCEQvF2u NGHHUpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm1OlkyPSd-Mki5OVY6OTV:L3G+
FS4-LTM cells M2D1PWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M1zKZlI1KGh? MVTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE[VND3MWG0h[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IGfTWE0yKGG|c3H5MEBKSzVyIE2gNk45KM7:TT6= M2raT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUW2PVE2Lz5{OEm1OlkyPTxxYU6=
Vero cells NFnNbYhEgXSxdH;4bYNqfHliYYPzZZk> MoHwNkBl[Xm| MUHDfZRwfG:6aXPpeJkh[WejaX7zeEBC\nKrY3HuJIdz\WWwIH3vcotmgSCYZYLvJINmdGy|IHHmeIVzKDJiZHH5d{BjgSCFZXzsWIl1\XJvR3zvJIx2dWmwZYPj[Y51KGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxKD1iMD6wNFI1KM7:TT6= NFnwflU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm5N|ExPid-Mki5PVMyODZ:L3G+
HepG2 cells MnfKR5l1d3SxeHnjbZR6KGG|c3H5 NUPnSFNTOTByIIXN MUWzJIRigXN? M{jNR2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGG2IEGwNEB2VSCjZoTldkA{KGSjeYOgZpkhS2WubGTpeIVzNUeubzDseY1qdmW|Y3XueEBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDBwMEG4JO69VS5? M4fSZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUmzNVA3Lz5{OEm5N|ExPjxxYU6=
NIH/3T3 cells NHnzPJZEgXSxdH;4bYNqfHliYYPzZZk> NV:5WXZLQTZiaB?= NH2xS|JEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBPUUhxM2SzJINmdGy|IHHmeIVzKDl4IHjyd{BjgSCVUlKgZZN{[XluIFXDOVAhRSByLkCwPEDPxE1w MmfuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzOUe3N|AoRjJ7MUm3O|MxRC:jPh?=
518A2 cells MXrDfZRwfG:6aXPpeJkh[XO|YYm= MV[5OkBp MmDUR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gOVE5STJiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhTUN3MDC9JFAvODNizszNMi=> Mn[5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzOUe3N|AoRjJ7MUm3O|MxRC:jPh?=
A549 cells NV[zOXdoS3m2b4TvfIlkcXS7IHHzd4F6 NG[1NZk6PiCq NFjwWo1EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEWFNUCgQUAxNjB2IN88UU4> MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF7N{ezNEc,OjlzOUe3N|A9N2F-
MCF7 cells MYXDfZRwfG:6aXPpeJkh[XO|YYm= M2jIelk3KGh? NUS3WGhHS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCHQ{WwJF0hOC5zIN88UU4> MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF7N{ezNEc,OjlzOUe3N|A9N2F-
A2780 cells M{[4b2N6fG:2b4jpZ4l1gSCjc4PhfS=> MX65OkBp MY\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBNlc5OCClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBGSzVyIE2gNE4yOiEQvF2u NHTwR4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG5O|c{OCd-MkmxPVc4OzB:L3G+
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insect cells MXjGeY5kfGmxbjDhd5NigQ>? MWizNEBucW6| MlL1TY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCSRFfGVoJmfGFiZYjwdoV{e2WmIHnuJIlve2WldDDj[YxteyC3c3nu[{BWdGmpaIStVI9tgSCJQWTbSWF[MDF8MUqxLX1vKGG|IIP1ZpN1emG2ZTDh[pRmeiB|MDDtbY5{KGK7IFzBUmNGKG2ndHjv[EwhUUN3MDC9JFAvODByN{[g{txONg>? M4PkSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUS5PFM3Lz5{OUW0PVg{PjxxYU6=
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insect cells NFjQdm1HfW6ldHnvckBie3OjeR?= NILaWHEyPSCvaX7z NX\LVJBpUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDDSGsyN0O7Y3zpckBDKGW6cILld5Nm\CCrbjDpcpNm[3RiY3XscJMhfXOrbnegWYxq\2i2LVPGSmtPUV[WUGLUVHBRW1GJSz3hcYll\SCjczDzeYJ{fHKjdHWgZYZ1\XJiMUWgcYlveyCkeTDMRW5ETSCvZYToc4QtKEmFNUCgQUAxNjB{NTFOwG0v NVH6ZoxrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFk5OzZpPkK5OVQ6QDN4PD;hQi=>
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Sf9 cells NGDVUGdHfW6ldHnvckBie3OjeR?= M{TQU2lvcGmkaYTpc44hd2ZiTj30[ZJucW6jbDDGUGFINXSjZ3fl[EBpfW2jbjDmeYxtNWynbnf0bEBETEtzMzCoNUB1dyBzNUGyJJJme2mmdXXzLU9PNXSncn3pcoFtKEircz30ZYdo\WRiQ4njT{ApOSC2bzC1PFAhemW|aXT1[ZMqKGW6cILld5Nm\CCrbjDT[lkh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIFHUVE1l\XCnbnTlcpQhXUyrZ3j0MVRGNUKSMTCoWIhzOzdxVHjyOFYqKHO3YoP0doF1\SCyZYD0bYRmKHCqb4PwbI9zgWyjdHnvckBxeixiSVO1NEA:KDBwMEW3JO69VS5? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODB4N{O1PEc,OzByNkezOVg9N2F-
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アッセイ
Methods Test Index PMID
Western blot
FAK / RIP; 

PubMed: 15121855     


Western blot (WB) analysis of BT-20 and BT-474 lysates treated with staurosporine (200 nM). Control cells were treated with dimethyl sulfoxide for 18 h.

p-Akt / Akt / PARP /Cleaved PARP; 

PubMed: 24174874     


Western blot analysis for Akt, P-Akt, and cleaved poly(ADP-ribose) polymerase. U87 cells were incubated with 200 nM, 10 nM, and 0.1 nM of staurosporine encapsulated in liposomes and staurosporine for 32 hours.

Ambra1; 

PubMed: 24587252     


SW620 cells treated with various doses of staurosporine for 6 h. Ambra1 levels were measured by Western blot. 

15121855 24174874 24587252
Growth inhibition assay
Cell viability; 

PubMed: 25215174     


Staurosporine reduces cerebellar astrocytes viability. (a) Cerebellar astrocytes were treated with staurosporine 0.1 μM, 0.25 μM, and 0.5 μM for 24 h and the cell viability was measured by MTT transformation. (b) Cerebellar astrocytes were treated with staurosporine 0.5 μM for 6, 12, and 24 h and the cell viability was measured by MTT transformation. Data are presented as mean ± SEM of four independent experiments. ∗ is significantly different from control (P < 0.05).

25215174
Immunofluorescence
Phalloidin / Type II collagen; 

PubMed: 22684244     


Cell were cultured in the absence (a-f) or presence of staurosporine (STSN, 5 × 10-9M) or cytochalasin D (CD, 1 µg/ml) for 1 (upper panel) or 2 (lower panel) days at low density and stained for F-actin (Phalloidin) and type II collagen (Type II). STSN: Staurosporine.

Tubulin / Actin; 

PubMed: 25215174     


Morphological changes of astrocytes induced by St are evidenced by the rearrangement of cytoskeletal proteins. Astrocytes were treated with St (0.5 μM) for 12 hours and then were labelled with rhodamine-phalloidin or immunostained for tubulin. Representative images of phase contrast, rhodamine-phalloidin, and tubulin are shown in control and St treated astrocytes. Scale bar represents 50 μm.

FAK 4.47; 

PubMed: 15121855     


BT-20 or BT-474 cell culture were plated on six-well plates and were treated with staurosporine (200 nM) after 24 h. After 6 h of treatment, cells were immunostained with anti-FAK 4.47 antibody (white arrowheads mark some focal adhesions).

cleaved-caspase 3; 

PubMed: 19840952     


Representative images (×1000 magnification) showing vehicle treated (C) and staurosporine treated (D) cells stained with anti-cleaved caspase 3 antibody and DAPI.

Annexin; 

PubMed: 15140398     


Induction of apoptosis with staurosporine resulting in activation of the ICE-NIRF probe. Gli36 cells were treated with 50 µM staurosporine for 24 hours (A-C) or with the same percentage of DMSO (0.01%) to which experimental wells were exposed (D-488nm laser, E-633nm laser and F-bright field). To examine the role of caspase-1 in staurosporine-induced apoptosis and probe activation, cells were coincubated in caspase-1 inhibitor (10 µM) and staurosporine (G-I). Staurosporine induces apoptosis, indicated by the positive annexin staining viewed with the 488-nm laser (A), which colocalized with activated probe viewed with the 633-nm laser (B). Coincubation of the caspase-1 inhibitor with staurosporine did not completely block apoptosis, indicated by the relatively higher number of apoptotic cells stained with annexin (G), as those that activated the probe (H). Magnification, x 40; scale bar, 50 µM.

Pyk2; 

PubMed: 19880522     


Pyk2 translocates to the nucleus upon staurosporine addition to ID8 cells. Confocal immunofluorescent analysis of endogenous Pyk2 upon DMSO (control) or 1 μm staurosporine addition for 2 h. The scale bar is 10 μm. 

22684244 25215174 15121855 19840952 15140398 19880522
体内試験 In the gerbil and rat ischemia models, Staurosporine pretreatment (0.1-10 ng) before ischemia prevents neuronal damage in a dose-dependent manner, suggesting the involvement of PKC in CAl pyramidal cell death after ischemia. [8]

お薦めの試験操作(参考用のみ)

キナーゼ試験:

[1]

- 合併

Enzyme assay and binding assay:

Protein kinase C is assayed in a reaction mixture (0.25 mL) containing 5 μmol of Tris/HCl, pH 7.5, 2.5 μmol of magnesium acetate, 50 μg of histone II S, 20 μg of phosphatidylserine, 0.88 μg of diolein, 125 nmol of CaCl2, 1.25 nmol of [γ-32]ATP (5-10 × 104 cpm/nmol) and 5 μg of partially purified enzyme. The binding of [3H]PDBu to protein kinase C is determined: Reaction mixture (200 μL contained 4 μmo1 of Tris/malate, pH 6.8, 20 μmol of KCl, 30 nmol of CaC12, 20 μg of phosphatidylserine, 5 μg of partially purified protein kinase C, 0.5% (final concentration) of DMSO,10 pmol of [3H]PDBu (l-3 × 104 cpm/pmol) and 10 μL of various amounts of Staurosporine.
細胞試験:

[3]

- 合併
  • 細胞株: PC12
  • 濃度: Dissolved in DMSO, final concentration 1 μM
  • 反応時間: ~32 hours
  • 実験の流れ:

    Cells are exposed to Staurosporine for ~32 hours. Cells are fixed in 4% paraformaldehyde and stained with the DNA-binding dye Hoechst 33342. Cells are visualized under epifluorescence illumination, and the percentage of apoptotic cells (cells with condensed and fragmented DNA) is determined.


    (参考用のみ)
動物試験:

[8]

- 合併
  • 動物モデル: Male Mongolian gerbils or male Wistar rats subjected to transient ischemia
  • 投薬量: ~10 ng
  • 投与方法: Stereotaxically administered into the bilateral CAl subfield of the hippocampus
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 4 mg/mL (8.57 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。

化学情報

分子量 466.53
化学式

C28H26N4O3

CAS No. 62996-74-1
Storage powder
in solvent
別名 Antibiotic AM-2282, STS
Smiles CC12C(C(CC(O1)N3C4=CC=CC=C4C5=C6C(=C7C8=CC=CC=C8N2C7=C53)CNC6=O)NC)OC

投与溶媒組成計算器(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)
投与量 mg/kg 動物平均体重 g 投与体積(動物毎) ul 動物数
ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)
% DMSO % % Tween 80 % ddH2O
計算リセット

便利ツール

モル濃度計算器

モル濃度計算器

求めたい質量、体積または濃度を計算してください。

質量 (mg) = 濃度 (mM) x 体積 (mL) x 分子量 (g/mol)

モル濃度計算器方程式

  • 質量
    濃度
    体積
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備するために必要な希釈率を計算してください。Selleck希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 入力 出力 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

質量 濃度 体積 分子量

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

  • * 必須
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