Staurosporine

For research use only. Not for use in humans.

製品コードS1421 別名:CGP 41251, Antibiotic AM-2282, STS, AM-2282

Staurosporine化学構造

CAS No. 62996-74-1

Staurosporine (CGP 41251, Antibiotic AM-2282, STS, AM-2282) is a potent PKC inhibitor for PKCα, PKCγ and PKCη with IC50 of 2 nM, 5 nM and 4 nM, less potent to PKCδ (20 nM), PKCε (73 nM) and little active to PKCζ (1086 nM) in cell-free assays. Also shows inhibitory activities on other kinases, such as PKA, PKG, S6K, CaMKII, etc. Phase 3.

サイズ 価格(税別)  
JPY 46800
JPY 85000
JPY 216560
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バルク問合せ

文献中Selleckの製品使用例(91)

製品安全説明書

Antineoplastic and Immunosuppressive Antibiotics阻害剤の選択性比較

生物活性

製品説明 Staurosporine (CGP 41251, Antibiotic AM-2282, STS, AM-2282) is a potent PKC inhibitor for PKCα, PKCγ and PKCη with IC50 of 2 nM, 5 nM and 4 nM, less potent to PKCδ (20 nM), PKCε (73 nM) and little active to PKCζ (1086 nM) in cell-free assays. Also shows inhibitory activities on other kinases, such as PKA, PKG, S6K, CaMKII, etc. Phase 3.
ターゲット
PKCα [1]
(Cell-free assay)
c-Fgr [2]
(Cell-free assay)
phosphorylase kinase [2]
(Cell-free assay)
PKCη [1]
(Cell-free assay)
PKCγ [1]
(Cell-free assay)
2 nM 2 nM 3 nM 4 nM 5 nM
体外試験

Staurosporine, a microbial alkaloid, significantly inhibits protein kinase C from rat brain with IC50 of 2.7 nM. Staurosporine displays strong inhibitory effect against HeLa S3 cells with IC50 of 4 nM. [1] Staurosporine also inhibits a variety of other protein kinases, including PKA, PKG, phosphorylase kinase, S6 kinase, Myosin light chain kinase (MLCK), CAM PKII, cdc2, v-Src, Lyn, c-Fgr, and Syk with IC50 of 15 nM, 18 nM, 3 nM, 5 nM, 21 nM, 20 nM, 9 nM, 6 nM, 20 nM, 2 nM, and 16 nM, respectively. [2] Staurosporine (1 μM) induces >90% apoptosis in PC12 cells. Consistently, Staurosporine treatment induces a rapid and prolonged elevation of intracellular free calcium levels [Ca2+]i, accumulation of mitochondrial reactive oxygen species (ROS), and subsequent mitochondrial dysfunction. [3] The apoptosis of MCF7 cells induced by Staurosporine can be enhanced by the expression of functional caspase-3 via caspase-8 activation and Bid cleavage. [4] Staurosporine treatment at 1 μM only partially inhibits IL-3-stimulated Bcl2 phosphorylation but completely blocks PKC-mediated Bcl2 phosphorylation. [5] Staurosporine induces apoptosis of human foreskin fibroblasts AG-1518, depending on the lysosomal cathepsins D mediated cytochrome c release and caspase activation. [6] In addition to activating the classical mitochondrial apoptosis pathway, Staurosporine triggers a novel intrinsic apoptosis pathway, relying on the activation of caspase-9 in the absence of Apaf-1. [7]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human colon carcinoma cell line HCT116 MVTGeY5kfGmxbjDhd5NigQ>? MXHDc45k\W62cnH0bY9vKHKncYXpdoVlKG[xcjDndo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDjc4xwdiClYYLjbY5wdWFiY3XscEBtcW6nIFjDWFEyPixiSVO1NF03KG6PLh?= NVKxUYFXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW1N|c{PDVpPkG1OVM4OzR3PD;hQi=>
human PBMC MVfGeY5kfGmxbjDhd5NigQ>? MlLjNlQhcA>? NYDHe2RiW3WycILld5Nqd25ib3[gTWwzKHC{b3T1Z5Rqd25iaX6gbJVu[W5iUFLNR{Bi\nSncjCyOEBpenNiYomgSWxKW0FuIFnDOVA:OTZibl2u MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDV6NUC0Okc,OTh3OEWwOFY9N2F-
endothelial cells MmK4SpVv[3Srb36gZZN{[Xl? NGnwU4RG\m[nY4TpeoUh\G:|ZTDh[4FqdnO2IIDsZZNucW6xZ3XuJIFkfGm4YYTvdkBi[3Srdnn0fUB{fGmvdXzheIVlKGK7IIDoc5Jjd2xiZYP0[ZIhcW5iZX7kc5Rp\WyrYXygZ4VtdHNuIFXEOVAhRSB5LkWg{txONg>? Mn\LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDdyOUC5OUc,QDdyOUC5OVww[T5?
Sf9 insect cells NVP4PFZ3TnWwY4Tpc44h[XO|YYm= M3e4VGlvcGmkaYTpc44hd2ZicILveIVqdiCtaX7hd4UhSyhzOkHtbZh1fXKnIH;mJHBMSyCjbIDoZUAnKGKndHGpJIV5eHKnc4Pl[EBqdiCVZkmgbY5{\WO2IHPlcIx{NCCLQ{WwJF0hOC5yMjFOwG0v MkCzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTh5M{[wOUc,QTh5M{[wOVww[T5?
LoVo cells Mn[yRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MnGzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiClb3zvckBk[W6lZYKgZ4VtdCCuaX7lJEhNd1[xIHPlcIx{MSC3c3nu[{BOXFRiYYPzZZktKEmFNUCgQUAxNjByMTFOwG0v NFfjPWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUW5NVUxPSd-MUG1PVE2ODV:L3G+
umbilical vein endothelial cells MkS2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NH3kW3RCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIIXtZoltcWOjbDD2[YlvKGWwZH;0bIVtcWGuIHPlcIx{KCiKVW\FR5MqKHW|aX7nJG1VXCCjc4PhfUwhUUN3MDC9JFAvODB2IN88UU4> NYX4[JBNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG1PVE2ODVpPkGxOVkyPTB3PD;hQi=>
ST-486 cells M{Dqc2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NHTFWnJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIJ2emurdDDsfY1xcG:vYTDj[YxtKGyrbnWgLHNVNTR6NjDj[YxteylidYPpcochVVSWIHHzd4F6NCCLQ{WwJF0hOC5yMEeg{txONg>? M1rBNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzNUmxOVA2Lz5zMUW5NVUxPTxxYU6=
DLD-1 cells NI\PT5VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NHXwXHpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIHPvcI9vKGOjbnPldkBk\WyuIHzpcoUhMESORD2xJINmdGy|KTD1d4lv\yCPVGSgZZN{[XluIFnDOVAhRSByLkCwPUDPxE1w NXfsUmM{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG1PVE2ODVpPkGxOVkyPTB3PD;hQi=>
HEK293 cells MULGeY5kfGmxbjDhd5NigQ>? NE[1NW5KdmirYnn0bY9vKG:oIGDLR{1j\XSjIFnJJI1m\GmjdHXkJGlNNThicnXs[YF{\SCocn;tJGhGUzJ7MzDj[YxteyCkeTDFUGlUSSxiSVO1NEA:KDBwMEe3JO69VS5? M2HCWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{OUSxN|MyLz5zMkm0NVM{OTxxYU6=
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P19 cells M1T4R2Z2dmO2aX;uJIF{e2G7 M1r1WWlvcGmkaYTpc44hd2ZiUHzheIVt\XRvZHXybZZm\CCpcn;3eIgh\mGldH;yJJJm[2WydH;yJIlvKFBzOTDj[YxteyxiSVO1NEA:KDBwMECyJO69VS5? NGXCZnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe3NVQyQSd-MUW3O|E1OTl:L3G+
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P19 cells M3LueWZ2dmO2aX;uJIF{e2G7 MV3Jcohq[mm2aX;uJI9nKEOjbHPpeY0w[2GubX;keYxqdi2mZYDlcoRmdnRicILveIVqdiCtaX7hd4UhfHmyZTDJTUBqdiCSMUmgZ4VtdHNuIFnDOVAhRSByLkCwOkDPxE1w MkW2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5N{G0NVkoRjF3N{exOFE6RC:jPh?=
P19 cells NIXuTm9HfW6ldHnvckBie3OjeR?= NVnCV5VOUW6qaXLpeIlwdiCxZjDDfYNtcW5vZHXw[Y5l\W62IHvpcoF{\SBzIHnuJHAyQSClZXzsd{whUUN3MDC9JFAvODB6IN88UU4> MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd5MUSxPUc,OTV5N{G0NVk9N2F-
P19 cells NWnzbolYTnWwY4Tpc44h[XO|YYm= MkX4TY5pcWKrdHnvckBw\iCYYYPjeYxieiCnbnTveIhmdGmjbDDndo94fGhiZnHjeI9zKHKnY3XweI9zKGmwIGCxPUBk\WyuczygTWM2OCB;IECuNFE1KM7:TT6= NHzpeoc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe3NVQyQSd-MUW3O|E1OTl:L3G+
HEK293 cells MlTjSpVv[3Srb36gZZN{[Xl? Mk\vTY5pcWKrdHnvckBw\iCLTD24JJJmdGWjc3WgZpkhUEWNMkmzJINmdGy|IHX4dJJme3OrbnegVGtENWKndHGyMEBKSzVyIE2gNE4xPzdizszNMi=> NHPkemU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe3NVQyQSd-MUW3O|E1OTl:L3G+
P19 cells MULGeY5kfGmxbjDhd5NigQ>? MkO2TY5pcWKrdHnvckBw\iCLboP1cIlvKHKnY3XweI9zKGurbnHz[UBqdiCSMUmgZ4VtdHNuIFnDOVAhRSByLkKg{txONg>? MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd5MUSxPUc,OTV5N{G0NVk9N2F-
P19 cells MWrGeY5kfGmxbjDhd5NigQ>? M4\3V2lvcGmkaYTpc44hd2ZiQ3Hz[YlvKGurbnHz[UAyKGmwIGCxPUBk\WyuczygTWM2OCB;IEGuOEDPxE1w NYrTb41jRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3O|E1OTlpPkG1O|cyPDF7PD;hQi=>
FL5.12-Akt1 cells MnTsRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? Ml;tRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDGUFUvOTJvQXv0NUBk\WyuczDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjJ7IN88UU4> MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjRyM{[yOkc,OTZ2MEO2NlY9N2F-
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FL5.12-Akt1 cells NGC4S3dHfW6ldHnvckBie3OjeR?= M2fXOGFkfGm4aYT5JIFo[Wmwc4SgS3NMOyCrbjDGUFUvOTJvQXv0NUBk\WyuczygSWM2OCB;IECuOFYh|ryPLh?= MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjRyM{[yOkc,OTZ2MEO2NlY9N2F-
MiaPaCa-2 cells Mkm5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MWrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2rYWDhR4EuOiClZXzsd{whUUN3MDC9JFAvOzdizszNMi=> MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjRzM{e4NEc,OTZ2MUO3PFA9N2F-
FL5.12-Akt1 cells NUj0[Vl1TnWwY4Tpc44h[XO|YYm= NHmyemdC[3Srdnn0fUBi\2GrboP0JGdUUzNicHjvd5Bpd3K7bHH0bY9vKGmwIF\MOU4yOi2Da4SxJINmdGy|LDDFR|UxKD1iMD60OkDPxE1w M{fPW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NEGzO|gxLz5zNkSxN|c5ODxxYU6=
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insect Sf21 cells NGPWcphHfW6ldHnvckBie3OjeR?= NYTyVVhQUW6qaXLpeIlwdiCxZjDKRWs{KGW6cILld5Nm\CCrbjDpcpNm[3RiU3[yNUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHLpc5RqdnmuYYTl[EB{fWK|dILheIUheGixc4Doc5J6dGG2aX;uMEBKSzVyIE2gNE4xODZizszNMi=> MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR{N{KwN{c,OTl2MkeyNFM9N2F-
Jurkat cells M{L1bWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NFvzRldCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGpCUzNiZYjwdoV{e2mwZzDJUFIue3SrbYXsZZRm\CCqdX3hckBLfXKtYYSgZ4VtdHNuIFnDOVAhRSByLkC3NUDPxE1w NWCzVo13RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0NlczODNpPkG5OFI4OjB|PD;hQi=>
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MCF7 cells NXPnVYE5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NITUPWg1QCCq M3XGZmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMz6wO{DPxE1w NUTuN2RnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1NFM{PjRpPkKyOVA{OzZ2PD;hQi=>
A431 cells MV3DfZRwfG:6aXPpeJkh[XO|YYm= M3j3fVI1KGh? MknuR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVQ{OSClZXzsd{Bi\nSncjCyOEBpenNidYPpcochSW6wZYjpckBX\U[LVFOvdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZzDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjB5IN88UU4> NHnsT5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW0NVA2OSd-MkK1OFExPTF:L3G+
MCF7 cells NEPuXW1EgXSxdH;4bYNqfHliYYPzZZk> M{SwUFI1KGh? MoHkR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjCyOEBpenNidYPpcochSW6wZYjpckBX\U[LVFOvdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZzDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjF6IN88UU4> MmHYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3NEGwOVEoRjJ{NUSxNFUyRC:jPh?=
BJ cells MmTDR5l1d3SxeHnjbZR6KGG|c3H5 MoDMO|IhcA>? Mm\sR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRmoh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IFPhcINmcW5iQV2gZZN{[XluIFnDOVAhRSByLkCwNkDPxE1w NHjvTWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyNVA5OSd-MkK5NlExQDF:L3G+
CEM cells Mk\qR5l1d3SxeHnjbZR6KGG|c3H5 NUe3SVF5PzJiaB?= NFvp[FFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBETU1iY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGNidGOnaX6gRW0h[XO|YYmsJGlEPTBiPTCwMlAzOyEQvF2u MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl{MUC4NUc,OjJ7MkGwPFE9N2F-
MCF7 cells MVLDfZRwfG:6aXPpeJkh[XO|YYm= NGPiT484OiCq NIjQUWVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFczKGi{czDifUBE[WylZXnuJGFOKGG|c3H5MEBKSzVyIE2gNE4xPjRizszNMi=> MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl{MUC4NUc,OjJ7MkGwPFE9N2F-
HeLa cells MkDrR5l1d3SxeHnjbZR6KGG|c3H5 Mlm1O|IhcA>? MYHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDDZYxk\WmwIFHNJIF{e2G7LDDJR|UxKD1iMD6xO|Uh|ryPLh?= MlfoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7MkGwPFEoRjJ{OUKxNFgyRC:jPh?=
HepG2 cells MojWSpVv[3Srb36gZZN{[Xl? MYOyOEBp MV7Jcohq[mm2aX;uJI9nKHSnbH;t[ZJie2ViaX6gbJVu[W5iSHXwS|Ih[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IGTSRXAuWEOULVXMTXNCKGG|c3H5MEBKSzVyIE2gOE4yPCEQvF2u M4DUOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEG4NFk3Lz5{M{CxPFA6PjxxYU6=
SW1116 cells MXHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M{nzR|Q5KGh? M4D1OWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU2exNVE3KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQhdWW2aH;kMEBKSzVyIE2gOE46PSEQvF2u Mmf2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMUiwPVYoRjJ|MEG4NFk3RC:jPh?=
HepG2 cells MlTBRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? Mn;tOFghcA>? Mo\3RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZYDHNkBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJI1mfGixZDygTWM2OCB;IE[uO|Mh|ryPLh?= NFXp[FM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CxPFA6Pid-MkOwNVgxQTZ:L3G+
BGC823 cells NVLpdXVtSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NEHkcII1QCCq M1y5WWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQlfDPFI{KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQhdWW2aH;kMEBKSzVyIE2gOk45OyEQvF2u MmG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMUiwPVYoRjJ|MEG4NFk3RC:jPh?=
HeLa cells MYnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NWDucZd{PDhiaB?= NGW1OpNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjlUIEh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBu\XSqb3SsJGlEPTBiPTC5MlEzKM7:TT6= Ml7LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMUiwPVYoRjJ|MEG4NFk3RC:jPh?=
HCT116 cells M4C0V2Z2dmO2aX;uJIF{e2G7 NE\OXZBKdmirYnn0bY9vKG:oIGTOTWsudWWmaXH0[YQhXEOIND;i[ZRiNWOjc3XpckB1emGwc3PybZB1cW:wIHnuJIh2dWGwIFjDWFEyPiClZXzsd{BjgSCkZYThMYxi[3SjbXHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwJF0hOC5yM{mg{txONg>? MorqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{M{KwOlAoRjJ|MkOyNFYxRC:jPh?=
HepG2 cells NH3xWmZHfW6ldHnvckBie3OjeR?= NGDPeYg1QCCq NU\rd2F4UW6qaXLpeIlwdiCxZjD0[YxwdWW{YYPlJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFQ5KGi{czDifUBVWkGSLWDDVk1GVEmVQTygTWM2OCB;IEiuN{DPxE1w M1rJNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|Mke5PFY1Lz5{M{K3PVg3PDxxYU6=
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KE-97 cells NVzqUJZ{S3m2b4TvfIlkcXS7IHHzd4F6 MYq3NkBp MYTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLSU06PyClZXzsd{Bi\nSncjC3NkBpenNiYomgR4VtdFSrdILlMWdtdyCudX3pcoV{[2WwdDDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFAvOTNizszNMi=> MoTTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MkiyPFMoRjJ2M{K4Nlg{RC:jPh?=
Jurkat cells NFjFWWJEgXSxdH;4bYNqfHliYYPzZZk> NHvjOHo4OiCq M2S3TWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGp2emujdDDj[YxteyCjZoTldkA4OiCqcoOgZpkhS2WubGTpeJJmNUeubzDseY1qdmW|Y3XueEBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDBwMUSg{txONg>? NVXiS4ZGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNlgzQDNpPkK0N|I5Ojh|PD;hQi=>
HuH7 cells MUjDfZRwfG:6aXPpeJkh[XO|YYm= MWO3NkBp Mm[1R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVJPyClZXzsd{Bi\nSncjC3NkBpenNiYomgR4VtdFSrdILlMWdtdyCudX3pcoV{[2WwdDDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFAvOjNizszNMi=> MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN{OEK4N{c,OjR|MkiyPFM9N2F-
BGC823 cells NXjz[WxES3m2b4TvfIlkcXS7IHHzd4F6 NHXSRpo4OiCq MVLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDCS2M5OjNiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGNmdGyWaYTy[U1IdG9ibIXtbY5me2OnboSgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAxNjN6IN88UU4> MnfWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MkiyPFMoRjJ2M{K4Nlg{RC:jPh?=
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A2780 cells NGHl[HREgXSxdH;4bYNqfHliYYPzZZk> MUG5OkBp M4r1PGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzPzhyIHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEmFNUCgQUAxNjJizszNMi=> NUi3ZmNqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlE2OjFpPkK0N|YyPTJzPD;hQi=>
HT-29 cells NYPOcY97S3m2b4TvfIlkcXS7IHHzd4F6 NEfzS|M6PiCq MWrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIWE0zQSClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyIE2gNE4zKM7:TT6= NX;kWWM3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlE2OjFpPkK0N|YyPTJzPD;hQi=>
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MCF7 cells NU[wN|FIS3m2b4TvfIlkcXS7IHHzd4F6 M2jiSFk3KGh? M4rOcmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFAvPCEQvF2u NXvPOWI1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlE2OjFpPkK0N|YyPTJzPD;hQi=>
A549 cells M33tb2N6fG:2b4jpZ4l1gSCjc4PhfS=> NWLyN2RlQTZiaB?= M3\qe2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFAvPiEQvF2u NETY[Yo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2NVUzOSd-MkSzOlE2OjF:L3G+
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HeLa cells NIrZbXBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M{foWVI1KHSxIES4JIg> M{O2R2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSHXMZUBk\WyuczDh[pRmeiB{NDD0c{A1QCCqcoOgZpkhS0ONLUigZZN{[XluIFnDOVAhRSB{LkeyJO69VS5? NXS2b441RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3PVI5OTFpPkK0O|kzQDFzPD;hQi=>
A549 cells NH[2OmtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NF\PXVgzPCC2bzC0PEBp MnXERY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNUS5JINmdGy|IHHmeIVzKDJ2IITvJFQ5KGi{czDifUBES0tvODDhd5NigSxiSVO1NEA:KDNwMEWg{txONg>? M3z1clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{myPFEyLz5{NEe5NlgyOTxxYU6=
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Raji cells NFzLb41EgXSxdH;4bYNqfHliYYPzZZk> NUHBNJhoS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWmGsaTDj[YxteyxiRVO1NEA:KDJizszNMi=> NYHIfpVuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNVY{OTdpPkK1N|E3OzF5PD;hQi=>
BJ cells MV;DfZRwfG:6aXPpeJkh[XO|YYm= NVvjOVRtS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSkpiY3XscJMtKEWFNUCgQUAzKM7:TT6= M1n0UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{G2N|E4Lz5{NUOxOlMyPzxxYU6=
HEK293 cells NHzuZXZEgXSxdH;4bYNqfHliYYPzZZk> MXnDfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{OUOgZ4VtdHNuIFXDOVAhRSB{IN88UU4> NVr0V5hrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNVY{OTdpPkK1N|E3OzF5PD;hQi=>
A549 cells M3juOWN6fG:2b4jpZ4l1gSCjc4PhfS=> M3jKeFQ5KGh? NEW2[lVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjB{IN88UU4> M2jEU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEK1PVM1Lz5{NUiyOVk{PDxxYU6=
HeLa cells MUfDfZRwfG:6aXPpeJkh[XO|YYm= M4q1WlQ5KGh? NUjjXJNTS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWOYTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5yMkWg{txONg>? NX\4TphPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4NlU6OzRpPkK1PFI2QTN2PD;hQi=>
PC3 cells MmrIR5l1d3SxeHnjbZR6KGG|c3H5 NXjPOI8zPDhiaB?= M1iy[2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHBEOyClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4xOzFizszNMi=> MkHvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6MkW5N|QoRjJ3OEK1PVM1RC:jPh?=
MGC-803 cells M4ftcmZ2dmO2aX;uJIF{e2G7 NX3OfFM4OjRiaB?= NFPPPHVKdmirYnn0bY9vKG:oIITlcI9u\XKjc3WgbY4hcHWvYX6gUWdENThyMzDj[YxteyCjZoTldkAzPCCqcoOgZpkhXFKDUD3QR3IuTUyLU1GgZZN{[XluIFnDOVAhRSB6Lkm3JO69VS5? MnruQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ7MEC2OVYoRjJ4OUCwOlU3RC:jPh?=
Sf9 insect cells NWf3fXZ7TnWwY4Tpc44h[XO|YYm= MVmxNEBucW6| M1ru[mlvcGmkaYTpc44hd2ZiTj30[ZJucW6jbDDHV3QhfGGpZ3XkJJJm[2:vYnnuZY51KG[3bHytcIVv\3SqIHj1cYFvKEOKS{Gg[ZhxemW|c3XkJIlvKGKjY4Xsc5ZqenW|IGPmPUBqdnOnY4SgZ4VtdHNiYX\0[ZIhOTBibXnud{BjgSCudX3pcoV{[2WwdDDBSHAuT2yxIHHzd4F6NCCLQ{WwJF0hOC5yMEGyJO69VS5? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzB6OUKxNUc,OjdyOEmyNVE9N2F-
Sf21 cells NHLjdWdHfW6ldHnvckBie3OjeR?= NG\PNGk3OCCvaX7z MUnJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEqDS{Kg[ZhxemW|c3XkJIlvKFOoMkGgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKFWuaXfoeE1ESUeDR1HJSXRFU0W\WWTWT2QheGixc4Doc5J6dGG2aX;uJJBz\S2rbnP1ZoF1\WRiYnXmc5JmKHO3YoP0doF1\SCjZHTpeIlwdiCjbnSgcYVie3W{ZXSgZYZ1\XJiNkCgcYlveyCkeTDMRW5ETSCmZYTlZ5Rqd25ibXX0bI9lNCCLQ{WwJF0hOC5yMEKg{txONg>? NWPubnRQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexN|c{PTlpPkK3NVM4OzV7PD;hQi=>
Neuro2a cells NWfOdVJtS3m2b4TvfIlkcXS7IHHzd4F6 MY[3NkBp M17ncGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJG5mfXKxMnGgZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwMEGxN{DPxE1w MojGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhzNUK0NlcoRjJ6MUWyOFI4RC:jPh?=
MDA-MB-231 cells M124WmZ2dmO2aX;uJIF{e2G7 MonGNVYhcA>? MnTJTY5pcWKrdHnvckBw\iC{ZYTpco9jdGG|dH;tZUBxem:2ZXnuJJBpd3OyaH;yfYxifGmxbjDpckBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHmeIVzKDF4IHjyd{BjgSCKb3XjbJN1KDN|M{SyJJN1[WmwaX7nJIJie2WmIH\seY9z\XOlZX7j[UBie3OjeTygTWM2OCB;IECuNFY3KM7:TT6= NXntSHZ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0N|E{PDJpPkK4OFMyOzR{PD;hQi=>
Sf9 insect cells M1myc2Z2dmO2aX;uJIF{e2G7 NVraPYxXOjBibXnudy=> NYTwTIhKUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDOMZRmem2rbnHscJkhT1OWLYTh[4dm\CCHR1\SJGw5PTiUL2S3PVBON0N5OUfTJJRzcXCuZTDteZRidnRiZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{KGmwIGPmPUBqdnOnY4SgZ4VtdHNicILlbY5kfWKjdHXkJIZweiB{MDDtbY5{KG[xbHzve4VlKGK7IHHk[Il1cW:wIH;mJHs{O1CfLVHUVEBu\WG|dYLl[EBi\nSncjCyJIhzeyCkeTDmbYx1\XJvYnnu[Ilv\yCvZYToc4QtKEmFNUCgQUAxNjByMEK5JO69VS5? M3zIUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEiyNVUyLz5{OES4NlE2OTxxYU6=
HEK293 cells MX3DfZRwfG:6aXPpeJkh[XO|YYm= NHHpNXk4OiCq MWLDfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4h[2WubDD2bYFjcWyrdImgZYZ1\XJiN{KgbJJ{KGK7IFPlcIxVcXSncjDHcI8hdHWvaX7ld4NmdnRiYYPzZZktKEOFNUCgQUAxNjByM{W0JO69VS5? MnXUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4MkS3NFEoRjJ6NkK0O|AyRC:jPh?=
BT549 cells MlP2R5l1d3SxeHnjbZR6KGG|c3H5 M1HxdlI1KGh? NHO3S2FEgXSxdH;4bYMh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDCWFU1QSClZXzsd{BqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlA5KM7:TT6= NX[4bo52RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki3NFU1OzJpPkK4O|A2PDN{PD;hQi=>
MDA-MB-468 cells MWjDfZRwfG:6aXPpeJkh[XO|YYm= M{jUclI1KGh? MorpR5l1d3SxeHnjJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTR4ODDj[YxteyCrbnP1ZoF1\WRiZn;yJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjF3IN88UU4> NV2zRnk2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki3NFU1OzJpPkK4O|A2PDN{PD;hQi=>
MDA-MB-231 cells Mmm0R5l1d3SxeHnjbZR6KGG|c3H5 NVrFPHpmOjRiaB?= MXTDfZRwfG:6aXOgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGmwY4XiZZRm\CCob4KgNlQhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvOjRizszNMi=> Mn3wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5MEW0N|IoRjJ6N{C1OFMzRC:jPh?=
Vero cells MkmxR5l1d3SxeHnjbZR6KGG|c3H5 MkfaNkBl[Xm| MUfDfZRwfG:6aXPpeJkh[WejaX7zeEBC\nKrY3HuJIdz\WWwIH3vcotmgSCYZYLvJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJZq[WKrbHn0fUBi\nSncjCyJIRigXNiYomgZ4VtdC22aYTldk1odG9ibIXtbY5me2OnboSgZZN{[XluIGTDOVAhRSByLkCwNlQh|ryPLh?= MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDd4M{[0OUc,Ojh5NkO2OFU9N2F-
L929 cells MoTnRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MVu1JIRigXN? Mmj3RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCOOUK5JINmdGy|IHHmeIVzKDViZHH5d{BjgSCZU2StNUBie3OjeTygTWM2OCB;IECuNkDPxE1w NFrjVVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm1OlkyPSd-Mki5OVY6OTV:L3G+
KB-3-1 cells MojJRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MkTtOUBl[Xm| MoTnRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCNQj2zMVEh[2WubIOgZYZ1\XJiNTDkZZl{KGK7IGfTWE0yKGG|c3H5MEBKSzVyIE2gNE4zKM7:TT6= NVP4VWo{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OVY6OTVpPkK4PVU3QTF3PD;hQi=>
MCF7 cells MlPKRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MnTZOUBl[Xm| MXvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYX\0[ZIhPSCmYYnzJIJ6KFeVVD2xJIF{e2G7LDDJR|UxKD1iMT64JO69VS5? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl3NkmxOUc,Ojh7NU[5NVU9N2F-
FS4-LTM cells MmT3RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NX7BNI8xOjRiaB?= NUDEN|lUSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDGV|QuVFSPIHPlcIx{KGGodHXyJFI1KGi{czDifUBYW1RvMTDhd5NigSxiSVO1NEA:KDJwODFOwG0v MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl3NkmxOUc,Ojh7NU[5NVU9N2F-
Vero cells NGPiUXVEgXSxdH;4bYNqfHliYYPzZZk> NIm5emEzKGSjeYO= NWH6SYVYS3m2b4TvfIlkcXS7IHHnZYlve3RiQX\ybYNidiCpcnXlckBud26tZYmgWoVzdyClZXzsd{Bi\nSncjCyJIRigXNiYomgR4VtdFSrdHXyMWdtdyCudX3pcoV{[2WwdDDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFAvODB{NDFOwG0v MkTaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7OUOxNFYoRjJ6OUmzNVA3RC:jPh?=
HepG2 cells NUTDbVJzS3m2b4TvfIlkcXS7IHHzd4F6 MWqxNFAhfU1? NWjoZolEOyCmYYnz M{XTcGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGG2IEGwNEB2VSCjZoTldkA{KGSjeYOgZpkhS2WubGTpeIVzNUeubzDseY1qdmW|Y3XueEBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDBwMEG4JO69VS5? NIHZR209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm5N|ExPid-Mki5PVMyODZ:L3G+
NIH/3T3 cells MX7DfZRwfG:6aXPpeJkh[XO|YYm= M1\FOlk3KGh? Mkj2R5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUmlJNzOWMzDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCHQ{WwJF0hOC5yMEig{txONg>? MlLuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzOUe3N|AoRjJ7MUm3O|MxRC:jPh?=
518A2 cells MnjSR5l1d3SxeHnjbZR6KGG|c3H5 NV3yNld{QTZiaB?= NWLBc|ZyS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hPTF6QUKgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KFOUQjDhd5NigSxiRVO1NEA:KDBwMEOg{txONg>? MmW5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzOUe3N|AoRjJ7MUm3O|MxRC:jPh?=
A549 cells NYPtUYlNS3m2b4TvfIlkcXS7IHHzd4F6 MmrLPVYhcA>? NXfhN2pSS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCHQ{WwJF0hOC5yNDFOwG0v Ml3iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzOUe3N|AoRjJ7MUm3O|MxRC:jPh?=
MCF7 cells M{HPNmN6fG:2b4jpZ4l1gSCjc4PhfS=> M{PjPVk3KGh? MlXIR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBGSzVyIE2gNE4yKM7:TT6= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF7N{ezNEc,OjlzOUe3N|A9N2F-
A2780 cells NGnJeWpEgXSxdH;4bYNqfHliYYPzZZk> M4HqW|k3KGh? NXrTfG5zS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTJ5OECgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KFOUQjDhd5NigSxiRVO1NEA:KDBwMUKg{txONg>? M2nzbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUm3O|MxLz5{OUG5O|c{ODxxYU6=
HT-29 cells NGr0c4pEgXSxdH;4bYNqfHliYYPzZZk> MoD2PVYhcA>? NGrpS|NEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJXC1{OTDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCHQ{WwJF0hOC5zNTFOwG0v NYPHZZJ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxPVc4OzBpPkK5NVk4PzNyPD;hQi=>
PC3 cells NEXqPINEgXSxdH;4bYNqfHliYYPzZZk> NFLjR2lEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBRSzNiY3XscJMh[nlic4Xs[o9zcG:mYX3pcoUhSiCjc4PhfUwhUUN3MDC9JFAvODF5IN88UU4> M{S3ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7M{i5NVIzLz5{OUO4PVEzOjxxYU6=
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insect cells NVnEN44{TnWwY4Tpc44h[XO|YYm= MYezNEBucW6| NVTibnBnUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDQSGdHWmKndHGg[ZhxemW|c3XkJIlvKGmwc3XjeEBk\WyuczD1d4lv\yCXbHnnbJQuWG:ueTDHRXRcTUG\KEG6NVoyMV2wIHHzJJN2[nO2cnH0[UBi\nSncjCzNEBucW6|IHL5JGxCVkOHIH3leIhw\CxiSVO1NEA:KDBwMECwO|Yh|ryPLh?= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV2OUizOkc,Ojl3NEm4N|Y9N2F-
Sf9 insect cells M1\ibWZ2dmO2aX;uJIF{e2G7 MVe2NEBucW6| MknyTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCNRGKg[ZhxemW|c3XkJIlvKFOoOTDpcpNm[3RiY3XscJMhfXOrbnegWYxq\2i2LVPBS2FISUmHVFTLSXl[XF[NRDDhd{B{fWK|dILheIUh[W[2ZYKgOlAhdWmwczDifUBNSU6FRTDt[ZRpd2RuIFnDOVAhRSByLkCwNlEh|ryPLh?= NIe1NHY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0PVg{Pid-Mkm1OFk5OzZ:L3G+
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insect cells MX\GeY5kfGmxbjDhd5NigQ>? NIHZbnAyPSCvaX7z NVraN5EyUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDDSGsyN0O7Y3zpckBDKGW6cILld5Nm\CCrbjDpcpNm[3RiY3XscJMhfXOrbnegWYxq\2i2LVPGSmtPUV[WUGLUVHBRW1GJSz3hcYll\SCjczDzeYJ{fHKjdHWgZYZ1\XJiMUWgcYlveyCkeTDMRW5ETSCvZYToc4QtKEmFNUCgQUAxNjB{NTFOwG0v NYjOZ3RQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFk5OzZpPkK5OVQ6QDN4PD;hQi=>
insect cells M1T1UGZ2dmO2aX;uJIF{e2G7 NV;tdIU2OTVibXnudy=> MXnJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKEOGS{GvR5lkdGmwIFKg[ZhxemW|c3XkJIlvKGmwc3XjeEBk\WyuczD1d4lv\yCXbHnnbJQuS0[IS17JWnRRWlSSUGDTVWdMNWGvaXTlJIF{KHO3YoP0doF1\SCjZoTldkAyPSCvaX7zJIJ6KEyDTlPFJI1mfGixZDygTWM2OCB;IECuNFI2KM7:TT6= MlzBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEm4N|YoRjJ7NUS5PFM3RC:jPh?=
Sf9 insect cells MoXySpVv[3Srb36gZZN{[Xl? NH7abG8yPSCvaX7z NYDUNVEzUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDGcJQyKGW6cILld5Nm\CCrbjDT[lkhcW6|ZXP0JINmdGy|IIXzbY5oKFWuaXfoeE1VUyCyZYD0bYRmKGG|IIP1ZpN1emG2ZTDh[pRmeiBzNTDtbY5{KGK7IFzBUmNGKG2ndHjv[EwhUUN3MDC9JFAvODN3IN88UU4> M1jT[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUS5PFM3Lz5{OUW0PVg{PjxxYU6=
Sf21 insect cells NFzLS4JHfW6ldHnvckBie3OjeR?= MkThNVUhdWmwcx?= NFrtflhKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIFH1do9z[S2DIHX4dJJme3OnZDDpckBU\jJzIHnud4VkfCClZXzsd{B2e2mwZzDVcIlocHRvUmLSV2xNTSCjczDzeYJ{fHKjdHWgZYZ1\XJiMUWgcYlveyCkeTDMRW5ETSCvZYToc4QtKEmFNUCgQUAxNjB|OTFOwG0v NEP2UnY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0PVg{Pid-Mkm1OFk5OzZ:L3G+
insect cells NIj3OGZHfW6ldHnvckBie3OjeR?= MmnnOlAhdWmwcx?= MkHQTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCDS2SxJIV5eHKnc4Pl[EBqdiCrboPlZ5Qh[2WubIOgeZNqdmdiQ2LFRpRq\GViYYOgd5Vje3S{YYTlJIFnfGW{IE[wJI1qdnNiYomgUGFPS0VibXX0bI9lNCCLQ{WwJF0hOC5yNU[g{txONg>? NH7nTZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0PVg{Pid-Mkm1OFk5OzZ:L3G+
Sf9 insect cells Mki2SpVv[3Srb36gZZN{[Xl? M4e0UVYxKG2rboO= NYPuVWM6UW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDGRWsh\XiycnXzd4VlKGmwIGPmPUBqdnOnY4SgZ4VtdHNidYPpcochXWyrZ3j0MXRMKHCncITp[IUh[XNic4Xid5Rz[XSnIHHmeIVzKDZyIH3pcpMh[nliTFHOR2UhdWW2aH;kMEBKSzVyIE2gNE4xPjJizszNMi=> Mn3nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3NEm4N|YoRjJ7NUS5PFM3RC:jPh?=
insect cells MnvJSpVv[3Srb36gZZN{[Xl? MYK2NEBucW6| M{jWRWlvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iQVLMJIV5eHKnc4Pl[EBqdiCrboPlZ5Qh[2WubIOgeZNqdmdiVXzp[4h1NVSNIIDldJRq\GViYYOgd5Vje3S{YYTlJIFnfGW{IE[wJI1qdnNiYomgUGFPS0VibXX0bI9lNCCLQ{WwJF0hOC5{OTFOwG0v MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV2OUizOkc,Ojl3NEm4N|Y9N2F-
insect cells NVz5NGZWTnWwY4Tpc44h[XO|YYm= MXq2NEBucW6| MnThTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiClLV3FWEBmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzJJV{cW6pIGXsbYdpfC2FQVfBS2FKTVSGS1XZXXRXU0RiYYOgd5Vje3S{YYTlJIFnfGW{IE[wJI1qdnNiYomgUGFPS0VibXX0bI9lNCCLQ{WwJF0hOC5|MTFOwG0v MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV2OUizOkc,Ojl3NEm4N|Y9N2F-
PC3 cells Mnf6R5l1d3SxeHnjbZR6KGG|c3H5 NX;LSlM5PzJiaB?= MXjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDQR|Mh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IGPSRkBie3OjeTygTWM2OCB;IECuNFM6KM7:TT6= NFzheGQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW1PFEyQSd-Mkm1OVgyOTl:L3G+
Sf9 cells M3nxe2Z2dmO2aX;uJIF{e2G7 MUO0OUBucW6| MmfsTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCFLYTldo1qdmGuIFjpd{11[WepZXSvUk11\XKvaX7hcEBIW1RvdHHn[4VlKEWJRmKgLFY3QCC2bzCxNlExKHKnc3nkeYV{MSCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMhcW6oZXP0[YQhW2Z7IHPlcIx{KHW|aX7nJHBwdHlvKFfseUwhXHm{KTDhd{B{fWK|dILheIUhdWWjc4Xy[YQh[W[2ZYKgOFUhdWmwdYTld{BjgSCNaX7hd4UuT2yxIHz1cYlv\XOlZX70JIF{e2G7LDDJR|UxKD1iMD6wOFch|ryPLh?= NGrhPW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUmwNlcyQSd-Mkm5NFI4OTl:L3G+
Sf9 cells M4jySGZ2dmO2aX;uJIF{e2G7 NV60c4VMPDVibXnudy=> MVnJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKE5vdHXycYlv[WxiR2PUMZRi\2enZDDISXIzKCh4N{mgeI8hOTJ3NTDy[ZNq\HWnczmg[ZhxemW|c3XkJIlvKGKjY4Xsc5ZqenW|IHnu[oVkfGWmIGPmPUBk\WyuczD1d4lv\yCSb3z5MUhIdHVuIGT5dkkh[XNic4Xid5Rz[XSnIH3lZZN2emWmIHHmeIVzKDR3IH3pcpV1\XNiYomgT4lv[XOnLVfsc{BtfW2rbnXzZ4VvfCCjc4PhfUwhUUN3MDC9JFAvODZyODFOwG0v MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTlyMkexPUc,Ojl7MEK3NVk9N2F-
HT-29 cells NVzVR|VLTnWwY4Tpc44h[XO|YYm= NFPISmQ{KGh? NH;UWJhKdmirYnn0bY9vKG:oIH3peI9kcG:wZILpZYwhdWWvYoLhcoUheG:2ZX70bYFtKGmwIHj1cYFvKEiWLUK5JINmdGy|IHHmeIVzKDNiaILzJIJ6KEqFLUGgd5RicW6rbnegZoF{\WRiZnz1c5Jme2OnbnPlJIF{e2G7LDDJR|UxKD1iMD6wNkDPxE1w NH[2U4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEC1O|E2PSd-M{CwOVcyPTV:L3G+
Sf9 cells NXXEe5RFTnWwY4Tpc44h[XO|YYm= MX7Jcohq[mm2aX;uJI9nKE5vdHXycYlv[WxiRlzBS{11[WepZXSgbJVu[W5iZoXscE1t\W6pdHigR2RMOTJiKEGgeI8hOTR7MDDy[ZNq\HWnczmvUk11\XKvaX7hcEBJcXNvdHHn[4VlKEO7Y1ugLFEhfG9iNUiwJJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iU3[5JINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCDVGCt[IVx\W6mZX70JHVNcWeqdD20SU1DWDFiKGTodlM4N1SqckS2LUB{fWK|dILheIUheGWydHnk[UBxcG:|cHjvdplt[XSrb36gdJItKEmFNUCgQUAxNjB3MzFOwG0v NG\TRlY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEC2O|M2QCd-M{CwOlc{PTh:L3G+
Sf9 cells NHPad5dHfW6ldHnvckBie3OjeR?= NGfQcHBKdmirYnn0bY9vKG:oIF6teIVzdWmwYXygSmxCTy22YXfn[YQhcHWvYX6g[pVtdC2uZX7neIghS0SNMUKgLFEhfG9iMUS5NEBz\XOrZIXld{kwVi22ZYLtbY5idCCKaYOteIFo\2WmIFP5Z2shMDFidH:gOVgxKHKnc3nkeYV{MSCneIDy[ZN{\WRiaX6gV4Y6KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDBWHAu\GWyZX7k[Y51KFWOaXfoeE01TS2EUEGgLHRpejN5L2TodlQ3MSC|dXLzeJJifGVicHXweIll\SCyaH;zdIhwenmuYYTpc44heHJuIFnDOVAhRSByLkC1N{DPxE1w Mn\lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzByNkezOVgoRjNyME[3N|U5RC:jPh?=
Sf9 cells MojaSpVv[3Srb36gZZN{[Xl? M1fN[GlvcGmkaYTpc44hd2ZiTj30[ZJucW6jbDDGUGFINXSjZ3fl[EBpfW2jbjDmeYxtNWynbnf0bEBETEtzMzCoNUB1dyBzNUGyJJJme2mmdXXzLU9PNXSncn3pcoFtKEircz30ZYdo\WRiQ4njT{ApOSC2bzC1PFAhemW|aXT1[ZMqKGW6cILld5Nm\CCrbjDT[lkh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIFHUVE1l\XCnbnTlcpQhXUyrZ3j0MVRGNUKSMTCoWIhzOzdxVHjyOFYqKHO3YoP0doF1\SCyZYD0bYRmKHCqb4PwbI9zgWyjdHnvckBxeixiSVO1NEA:KDBwMEW3JO69VS5? M171NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyME[3N|U5Lz5|MEC2O|M2QDxxYU6=
Sf9 cells NWntT5JXTnWwY4Tpc44h[XO|YYm= MVzJcohq[mm2aX;uJI9nKE5vdHXycYlv[WxiRlzBS{11[WepZXSgbJVu[W5iZoXscE1t\W6pdHigR2RMOTNiKEGgeI8hOTVzMjDy[ZNq\HWnczmvUk11\XKvaX7hcEBJcXNvdHHn[4VlKEO7Y1ugLFEhfG9iNUiwJJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iU3[5JINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCDVGCt[IVx\W6mZX70JHVNcWeqdD20SU1DWDFiKGTodlM4N1SqckS2LUB{fWK|dILheIUheGWydHnk[UBxcG:|cHjvdplt[XSrb36gdJItKEmFNUCgQUAxNjB3NzFOwG0v MmrBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzByNkezOVgoRjNyME[3N|U5RC:jPh?=
HCT116 cells MX3DfZRwfG:6aXPpeJkh[XO|YYm= M2ftc|Q5KGh? M4nHXGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG|ZTDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMD6wN{DPxE1w NFflZpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEGwOlI6OSd-M{CxNFYzQTF:L3G+
Huh7.5 cells MWTDfZRwfG:6aXPpeJkh[XO|YYm= M2j4OVQ5KGh? MnThR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVpPy53IHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLk[g{txONg>? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODFyNkK5NUc,OzBzME[yPVE9N2F-
K562 cells MlHVR5l1d3SxeHnjbZR6KGG|c3H5 NXmweJlXPDhiaB?= MoXxR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT|U3OiClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOS55IN88UU4> M3\hT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMUC2NlkyLz5|MEGwOlI6OTxxYU6=
LO2 cells NEjzOIhEgXSxdH;4bYNqfHliYYPzZZk> NELIc4s1QCCq MV3DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMU|Ih[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFkvPSEQvF2u NHj2VpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEGwOlI6OSd-M{CxNFYzQTF:L3G+

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Methods Test Index PMID
Western blot
p-Akt / Akt / PARP /Cleaved PARP; 

PubMed: 24174874     


Western blot analysis for Akt, P-Akt, and cleaved poly(ADP-ribose) polymerase. U87 cells were incubated with 200 nM, 10 nM, and 0.1 nM of staurosporine encapsulated in liposomes and staurosporine for 32 hours.

FAK / RIP; 

PubMed: 15121855     


Western blot (WB) analysis of BT-20 and BT-474 lysates treated with staurosporine (200 nM). Control cells were treated with dimethyl sulfoxide for 18 h.

Ambra1; 

PubMed: 24587252     


SW620 cells treated with various doses of staurosporine for 6 h. Ambra1 levels were measured by Western blot. 

24174874 15121855 24587252
Growth inhibition assay
Cell viability; 

PubMed: 25215174     


Staurosporine reduces cerebellar astrocytes viability. (a) Cerebellar astrocytes were treated with staurosporine 0.1 μM, 0.25 μM, and 0.5 μM for 24 h and the cell viability was measured by MTT transformation. (b) Cerebellar astrocytes were treated with staurosporine 0.5 μM for 6, 12, and 24 h and the cell viability was measured by MTT transformation. Data are presented as mean ± SEM of four independent experiments. ∗ is significantly different from control (P < 0.05).

25215174
Immunofluorescence
Tubulin / Actin; 

PubMed: 25215174     


Morphological changes of astrocytes induced by St are evidenced by the rearrangement of cytoskeletal proteins. Astrocytes were treated with St (0.5 μM) for 12 hours and then were labelled with rhodamine-phalloidin or immunostained for tubulin. Representative images of phase contrast, rhodamine-phalloidin, and tubulin are shown in control and St treated astrocytes. Scale bar represents 50 μm.

Phalloidin / Type II collagen; 

PubMed: 22684244     


Cell were cultured in the absence (a-f) or presence of staurosporine (STSN, 5 × 10-9M) or cytochalasin D (CD, 1 µg/ml) for 1 (upper panel) or 2 (lower panel) days at low density and stained for F-actin (Phalloidin) and type II collagen (Type II). STSN: Staurosporine.

Pyk2; 

PubMed: 19880522     


Pyk2 translocates to the nucleus upon staurosporine addition to ID8 cells. Confocal immunofluorescent analysis of endogenous Pyk2 upon DMSO (control) or 1 μm staurosporine addition for 2 h. The scale bar is 10 μm. 

Annexin; 

PubMed: 15140398     


Induction of apoptosis with staurosporine resulting in activation of the ICE-NIRF probe. Gli36 cells were treated with 50 µM staurosporine for 24 hours (A-C) or with the same percentage of DMSO (0.01%) to which experimental wells were exposed (D-488nm laser, E-633nm laser and F-bright field). To examine the role of caspase-1 in staurosporine-induced apoptosis and probe activation, cells were coincubated in caspase-1 inhibitor (10 µM) and staurosporine (G-I). Staurosporine induces apoptosis, indicated by the positive annexin staining viewed with the 488-nm laser (A), which colocalized with activated probe viewed with the 633-nm laser (B). Coincubation of the caspase-1 inhibitor with staurosporine did not completely block apoptosis, indicated by the relatively higher number of apoptotic cells stained with annexin (G), as those that activated the probe (H). Magnification, x 40; scale bar, 50 µM.

cleaved-caspase 3; 

PubMed: 19840952     


Representative images (×1000 magnification) showing vehicle treated (C) and staurosporine treated (D) cells stained with anti-cleaved caspase 3 antibody and DAPI.

FAK 4.47; 

PubMed: 15121855     


BT-20 or BT-474 cell culture were plated on six-well plates and were treated with staurosporine (200 nM) after 24 h. After 6 h of treatment, cells were immunostained with anti-FAK 4.47 antibody (white arrowheads mark some focal adhesions).

25215174 22684244 19880522 15140398 19840952 15121855
体内試験 In the gerbil and rat ischemia models, Staurosporine pretreatment (0.1-10 ng) before ischemia prevents neuronal damage in a dose-dependent manner, suggesting the involvement of PKC in CAl pyramidal cell death after ischemia. [8]

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キナーゼ試験:

[1]

- 合併

Enzyme assay and binding assay:

Protein kinase C is assayed in a reaction mixture (0.25 mL) containing 5 μmol of Tris/HCl, pH 7.5, 2.5 μmol of magnesium acetate, 50 μg of histone II S, 20 μg of phosphatidylserine, 0.88 μg of diolein, 125 nmol of CaCl2, 1.25 nmol of [γ-32]ATP (5-10 × 104 cpm/nmol) and 5 μg of partially purified enzyme. The binding of [3H]PDBu to protein kinase C is determined: Reaction mixture (200 μL contained 4 μmo1 of Tris/malate, pH 6.8, 20 μmol of KCl, 30 nmol of CaC12, 20 μg of phosphatidylserine, 5 μg of partially purified protein kinase C, 0.5% (final concentration) of DMSO,10 pmol of [3H]PDBu (l-3 × 104 cpm/pmol) and 10 μL of various amounts of Staurosporine.
細胞試験:

[3]

- 合併
  • 細胞株: PC12
  • 濃度: Dissolved in DMSO, final concentration 1 μM
  • 反応時間: ~32 hours
  • 実験の流れ:

    Cells are exposed to Staurosporine for ~32 hours. Cells are fixed in 4% paraformaldehyde and stained with the DNA-binding dye Hoechst 33342. Cells are visualized under epifluorescence illumination, and the percentage of apoptotic cells (cells with condensed and fragmented DNA) is determined.


    (参考用のみ)
動物試験:

[8]

- 合併
  • 動物モデル: Male Mongolian gerbils or male Wistar rats subjected to transient ischemia
  • 投薬量: ~10 ng
  • 投与方法: Stereotaxically administered into the bilateral CAl subfield of the hippocampus
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 4 mg/mL (8.57 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。

化学情報

分子量 466.53
化学式

C28H26N4O3

CAS No. 62996-74-1
Storage powder
in solvent
別名 CGP 41251, Antibiotic AM-2282, STS, AM-2282
Smiles CC12C(C(CC(O1)N3C4=CC=CC=C4C5=C6C(=C7C8=CC=CC=C8N2C7=C53)CNC6=O)NC)OC

投与溶媒組成計算器(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)
投与量 mg/kg 動物平均体重 g 投与体積(動物毎) ul 動物数
ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)
% DMSO % % Tween 80 % ddH2O
計算リセット

便利ツール

モル濃度計算器

モル濃度計算器

求めたい質量、体積または濃度を計算してください。

質量 (mg) = 濃度 (mM) x 体積 (mL) x 分子量 (g/mol)

モル濃度計算器方程式

  • 質量
    濃度
    体積
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備するために必要な希釈率を計算してください。Selleck希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 入力 出力 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

質量 濃度 体積 分子量

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

  • * 必須
Tags: Staurosporineを買う | Staurosporine ic50 | Staurosporine供給者 | Staurosporineを購入する | Staurosporine費用 | Staurosporine生産者 | オーダーStaurosporine | Staurosporine化学構造 | Staurosporine分子量 | Staurosporine代理店
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細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID