Staurosporine (AM-2282)

For research use only. Not for use in humans.

製品コードS1421 別名:Antibiotic AM-2282, STS

Staurosporine (AM-2282)化学構造

CAS No. 62996-74-1

Staurosporine (AM-2282, Antibiotic AM-2282, STS) is a potent PKC inhibitor for PKCα, PKCγ and PKCη with IC50 of 2 nM, 5 nM and 4 nM, less potent to PKCδ (20 nM), PKCε (73 nM) and little active to PKCζ (1086 nM) in cell-free assays. Also shows inhibitory activities on other kinases, such as PKA, PKG, S6K, CaMKII, etc. Phase 3.

サイズ 価格(税別)  
JPY 46800
JPY 85000
JPY 216560
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バルク問合せ

文献中Selleckの製品使用例(111)

製品安全説明書

Antineoplastic and Immunosuppressive Antibiotics阻害剤の選択性比較

生物活性

製品説明 Staurosporine (AM-2282, Antibiotic AM-2282, STS) is a potent PKC inhibitor for PKCα, PKCγ and PKCη with IC50 of 2 nM, 5 nM and 4 nM, less potent to PKCδ (20 nM), PKCε (73 nM) and little active to PKCζ (1086 nM) in cell-free assays. Also shows inhibitory activities on other kinases, such as PKA, PKG, S6K, CaMKII, etc. Phase 3.
ターゲット
PKCα [1]
(Cell-free assay)
c-Fgr [2]
(Cell-free assay)
phosphorylase kinase [2]
(Cell-free assay)
PKCη [1]
(Cell-free assay)
PKCγ [1]
(Cell-free assay)
2 nM 2 nM 3 nM 4 nM 5 nM
体外試験

Staurosporine, a microbial alkaloid, significantly inhibits protein kinase C from rat brain with IC50 of 2.7 nM. Staurosporine displays strong inhibitory effect against HeLa S3 cells with IC50 of 4 nM. [1] Staurosporine also inhibits a variety of other protein kinases, including PKA, PKG, phosphorylase kinase, S6 kinase, Myosin light chain kinase (MLCK), CAM PKII, cdc2, v-Src, Lyn, c-Fgr, and Syk with IC50 of 15 nM, 18 nM, 3 nM, 5 nM, 21 nM, 20 nM, 9 nM, 6 nM, 20 nM, 2 nM, and 16 nM, respectively. [2] Staurosporine (1 μM) induces >90% apoptosis in PC12 cells. Consistently, Staurosporine treatment induces a rapid and prolonged elevation of intracellular free calcium levels [Ca2+]i, accumulation of mitochondrial reactive oxygen species (ROS), and subsequent mitochondrial dysfunction. [3] The apoptosis of MCF7 cells induced by Staurosporine can be enhanced by the expression of functional caspase-3 via caspase-8 activation and Bid cleavage. [4] Staurosporine treatment at 1 μM only partially inhibits IL-3-stimulated Bcl2 phosphorylation but completely blocks PKC-mediated Bcl2 phosphorylation. [5] Staurosporine induces apoptosis of human foreskin fibroblasts AG-1518, depending on the lysosomal cathepsins D mediated cytochrome c release and caspase activation. [6] In addition to activating the classical mitochondrial apoptosis pathway, Staurosporine triggers a novel intrinsic apoptosis pathway, relying on the activation of caspase-9 in the absence of Apaf-1. [7]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human colon carcinoma cell line HCT116 MmXMSpVv[3Srb36gZZN{[Xl? NXL2emliS2:wY3XueJJifGmxbjDy[ZF2cXKnZDDmc5Ih\3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4h[2:ub36gZ4Fz[2mwb33hJINmdGxibHnu[UBJS1RzMU[sJGlEPTB;NjDuUU4> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTV|N{O0OUc,OTV3M{ezOFU9N2F-
human PBMC NEfJWplHfW6ldHnvckBie3OjeR?= NGezSZYzPCCq M3nSe3N2eHC{ZYPzbY9vKG:oIFnMNkBxem:mdXP0bY9vKGmwIHj1cYFvKFCETVOgZYZ1\XJiMkSgbJJ{KGK7IFXMTXNCNCCLQ{WwQVE3KG6PLh?= M2PDSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUi1NFQ3Lz5zOEW4OVA1PjxxYU6=
endothelial cells MYPGeY5kfGmxbjDhd5NigQ>? Mo\ISYZn\WO2aY\lJIRwe2ViYXfhbY5{fCCybHHzcYlvd2enbjDhZ5RqfmG2b4KgZYN1cX[rdImgd5RqdXWuYYTl[EBjgSCyaH;yZo9tKGW|dHXyJIlvKGWwZH;0bIVtcWGuIHPlcIx{NCCHREWwJF0hPy53IN88UU4> NVP1ZpFYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOEewPVA6PSd-OEewPVA6PTxxYU6=
Sf9 insect cells NVLmcnI4TnWwY4Tpc44h[XO|YYm= MnLtTY5pcWKrdHnvckBw\iCycn;0[YlvKGurbnHz[UBEMDF8MX3pfJR2emVib3[gVGtEKGGucHjhJEYh[mW2YTmg[ZhxemW|c3XkJIlvKFOoOTDpcpNm[3RiY3XscJMtKEmFNUCgQUAxNjB{IN88UU4> NV;YbpNYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUi3N|YxPSd-OUi3N|YxPTxxYU6=
LoVo cells MV3BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MoXQRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiClb3zvckBk[W6lZYKgZ4VtdCCuaX7lJEhNd1[xIHPlcIx{MSC3c3nu[{BOXFRiYYPzZZktKEmFNUCgQUAxNjByMTFOwG0v NHzybnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUW5NVUxPSd-MUG1PVE2ODV:L3G+
umbilical vein endothelial cells M33zSGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MkDURY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiC3bXLpcIlk[WxidnXpckBmdmSxdHjlcIlidCClZXzsd{ApUFWYRVPzLUB2e2mwZzDNWHQh[XO|YYmsJGlEPTBiPTCwMlAxPCEQvF2u NHX0eFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUW5NVUxPSd-MUG1PVE2ODV:L3G+
ST-486 cells NFztUG5CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NFvpV45CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIJ2emurdDDsfY1xcG:vYTDj[YxtKGyrbnWgLHNVNTR6NjDj[YxteylidYPpcochVVSWIHHzd4F6NCCLQ{WwJF0hOC5yMEeg{txONg>? MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTV7MUWwOUc,OTF3OUG1NFU9N2F-
DLD-1 cells MXfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NWPEflhrSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDjc4xwdiClYX7j[ZIh[2WubDDsbY5mKCiGTFStNUBk\WyuczmgeZNqdmdiTWTUJIF{e2G7LDDJR|UxKD1iMD6wNFkh|ryPLh?= MoCyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF3OUG1NFUoRjFzNUmxOVA2RC:jPh?=
HEK293 cells MkXYSpVv[3Srb36gZZN{[Xl? M4PlTmlvcGmkaYTpc44hd2ZiUFvDMYJmfGFiSVmgcYVlcWG2ZXSgTWwuQCC{ZXzlZZNmKG[{b32gTGVMOjl|IHPlcIx{KGK7IFXMTXNCNCCLQ{WwJF0hOC5yN{eg{txONg>? MnnlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ7NEGzN|EoRjF{OUSxN|MyRC:jPh?=
P19 cells NH3EcmlHfW6ldHnvckBie3OjeR?= M1TybmlvcGmkaYTpc44hd2ZiUHzheIVt\XRvZHXybZZm\CCpcn;3eIgh\mGldH;yJJJm[2WydH;yJIlvKFBzOTDj[YxteyxiSVO1NEA:KDBwMECyJO69VS5? MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd5MUSxPUc,OTV5N{G0NVk9N2F-
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P19 cells MV;GeY5kfGmxbjDhd5NigQ>? MYrJcohq[mm2aX;uJI9nKEOjbHPpeY0w[2GubX;keYxqdi2mZYDlcoRmdnRicILveIVqdiCtaX7hd4UhfHmyZTDJTUBqdiCSMUmgZ4VtdHNuIFnDOVAhRSByLkCwOkDPxE1w MmPtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5N{G0NVkoRjF3N{exOFE6RC:jPh?=
P19 cells NVT6VHliTnWwY4Tpc44h[XO|YYm= MYrJcohq[mm2aX;uJI9nKEO7Y3zpck1l\XCnbnTlcpQhc2mwYYPlJFEhcW5iUEG5JINmdGy|LDDJR|UxKD1iMD6wNFgh|ryPLh?= M3[4TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{exOFE6Lz5zNUe3NVQyQTxxYU6=
P19 cells MV\GeY5kfGmxbjDhd5NigQ>? MWnJcohq[mm2aX;uJI9nKF[jc3P1cIFzKGWwZH;0bIVtcWGuIHfyc5d1cCCoYXP0c5IhemWlZYD0c5IhcW5iUEG5JINmdGy|LDDJR|UxKD1iMD6wNVQh|ryPLh?= NH;Qbpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe3NVQyQSd-MUW3O|E1OTl:L3G+
HEK293 cells M{PqS2Z2dmO2aX;uJIF{e2G7 MY\Jcohq[mm2aX;uJI9nKEmOLUigdoVt\WG|ZTDifUBJTUt{OUOgZ4VtdHNiZYjwdoV{e2mwZzDQT2Mu[mW2YUKsJGlEPTBiPTCwMlA4PyEQvF2u MnrrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5N{G0NVkoRjF3N{exOFE6RC:jPh?=
P19 cells MVPGeY5kfGmxbjDhd5NigQ>? NInsPGRKdmirYnn0bY9vKG:oIFnud5VtcW5icnXj[ZB1d3Jia3nuZZNmKGmwIGCxPUBk\WyuczygTWM2OCB;IECuNkDPxE1w NYi3U5ZURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3O|E1OTlpPkG1O|cyPDF7PD;hQi=>
P19 cells MnrCSpVv[3Srb36gZZN{[Xl? MUXJcohq[mm2aX;uJI9nKEOjc3XpckBscW6jc3WgNUBqdiCSMUmgZ4VtdHNuIFnDOVAhRSBzLkSg{txONg>? MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd5MUSxPUc,OTV5N{G0NVk9N2F-
FL5.12-Akt1 cells NU\yUGI6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NHLUdIlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGZNPS5zMj3Bb5QyKGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwMkmg{txONg>? NGW5UlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkSwN|YzPid-MU[0NFM3OjZ:L3G+
MiaPaCa-2 cells MWLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NWrLfWd6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBOcWGSYVPhMVIh[2WubIOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5|NzFOwG0v NHLUcYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkSwN|YzPid-MU[0NFM3OjZ:L3G+
FL5.12-Akt1 cells MXrGeY5kfGmxbjDhd5NigQ>? MoTmRYN1cX[rdImgZYdicW6|dDDHV2s{KGmwIF\MOU4yOi2Da4SxJINmdGy|LDDFR|UxKD1iMD60OkDPxE1w MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjRyM{[yOkc,OTZ2MEO2NlY9N2F-
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MDA-MB-231 cells M4rxeGN6fG:2b4jpZ4l1gSCjc4PhfS=> NUHMcmhmPzJiaB?= M1OyZWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IIP1cIZwemixZHHtbY5mKEJibXX0bI9lNCCJSUWwJF0hOC5yMEexJO69VS5? NUfVPHZ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi0PFQ4PzVpPkG4OFg1Pzd3PD;hQi=>
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MCF7 cells M1XYc2Z2dmO2aX;uJIF{e2G7 NY\iNW86UW6qaXLpeIlwdiCxZjDQT2EhcW5iaIXtZY4hVUOINzDj[YxteyCkeTDhdpJigS2kYYPl[EBndHWxcnXzZ4Vv[2ViYYPzZZktKEmFNUCgQUAzKM7:TT6= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ3NkO2PUc,OTh4NU[zOlk9N2F-
Sf9 cells NUizcZRNTnWwY4Tpc44h[XO|YYm= M3vBXGlvcGmkaYTpc44hd2ZiaIXtZY4hW3mtIHX4dJJme3OnZDDpckBU\jliY3XscJMtKEmFNUCgQUAxNjByMzFOwG0v NH7ybpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEiyN|c5PCd-MUi4NlM4QDR:L3G+
Sf9 cells M2m5b2Z2dmO2aX;uJIF{e2G7 M1yxS2lvcGmkaYTpc44hd2ZiaIXtZY4hYkGSN{Cg[ZhxemW|c3XkJIlvKFOoOTDj[YxteyxiSVO1NEA:KDBwMEWzJO69VS5? NFPBcZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEiyN|c5PCd-MUi4NlM4QDR:L3G+
insect cells NXPJcYhwTnWwY4Tpc44h[XO|YYm= MXzJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KFCrbUGg[ZhxemW|c3XkJIlvKGmwc3XjeEBk\WyuczDifUBJXFKILDDJR|UxKD1iMD6wNUDPxE1w NIL6N4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUG3PVA4Pid-MUmxO|kxPzZ:L3G+
HT-29 cells MoH0RZBweHSxc3nzJIF{e2G7 MWWyJIg> MofvTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCKVD2yPUBk\WyuczDhd5Nme3OnZDDhd{BkcGGwZ3WgbY4hdWm2b3Poc45lemmjbDD0doFve22nbXLyZY5mKHCxdHXueIlidCCjZoTldkAzKGi{czDifUBLSzFic4ThbY5qdmdiYnHz[YQh\my3b4Lld4NmdmOnIHHzd4F6NCCHREWwJF0hOC5yMEK2JO69VS5? NIC4Rmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUSyNlIxPid-MUm0NlIzODZ:L3G+
insect Sf21 cells NFXHOW9HfW6ldHnvckBie3OjeR?= NUXZ[Is5UW6qaXLpeIlwdiCxZjDKRWs{KGW6cILld5Nm\CCrbjDpcpNm[3RiU3[yNUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHLpc5RqdnmuYYTl[EB{fWK|dILheIUheGixc4Doc5J6dGG2aX;uMEBKSzVyIE2gNE4xODZizszNMi=> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR{N{KwN{c,OTl2MkeyNFM9N2F-
Jurkat cells NF3LNXdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MVHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFrBT|Mh\XiycnXzd4lv\yCLTEKtd5RqdXWuYYTl[EBpfW2jbjDKeZJs[XRiY3XscJMtKEmFNUCgQUAxNjB5MTFOwG0v M1vRdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEK3NlA{Lz5zOUSyO|IxOzxxYU6=
Jurkat cells M1P3SmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MXfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFrBT|Mh\XiycnXzd4lv\yCqdX3hckBz\XO2aX7nJGp2emujdDDj[YxteyxiSVO1NEA:KDBwM{GxJO69VS5? M1n2dFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEK3NlA{Lz5zOUSyO|IxOzxxYU6=
HUE cells M1v6XGZ2dmO2aX;uJIF{e2G7 NIf0Tow6OCCvaX7z M2fB[2lvcGmkaYTpc44hd2ZiVlXHSnIzKGmwIFjVSUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIG\FS2YucW6mdXPl[EBifXSxcHjvd5Bpd3K7bHH0bY9vKHS{ZXH0[YQh\m:{IEmwJI1qdnNiYnXmc5JmKF[HR1[gZ4hidGynbnflJIJ6KEWOSWPBMEBKSzVyIE2gNE4xPyEQvF2u Mlf3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzN{CxOlMoRjJyMUewNVY{RC:jPh?=
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Sf21 cells M2K2fWZ2dmO2aX;uJIF{e2G7 NGPER2s{OCC3TR?= MWLJcohq[mm2aX;uJI9nKGi3bXHuJGNJUzJia3nuZZNmKGW6cILld5Nm\CCrbjDpcpNm[3RiU3[yNUBk\WyuczD1d4lv\yCyaH;zdIhwNUOURVL0bYRmKGG|IIP1ZpN1emG2ZTDheEA{OCC3TTygTWM2OCB;IECuNFI4KM7:TT6= NVHvR4FURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN|Q4QTZpPkKxN|M1Pzl4PD;hQi=>
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HeLa cells MYDDfZRwfG:6aXPpeJkh[XO|YYm= M4PhNFQ5KGh? Mln0R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4xODByMESg{txONg>? MoLHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2OEi2OVUoRjJzNEi4OlU2RC:jPh?=
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HT-29 cells MVrGeY5kfGmxbjDhd5NigQ>? M3nmfGlvcGmkaYTpc44hd2ZibXn0c4Npd26mcnnhcEBu\W2kcnHu[UBxd3SnboTpZYwhcW5iaIXtZY4hUFRvMkmgZ4VtdHNidYPpcochUkNzIHT5[UB{fGGrbnnu[{BjgSCobIXvdoV{[2WwY3WgdIxifGVicnXh[IVzKGG|c3H5MEBKSzVyIE2gNE4xODJ3IN88UU4> NV:yWlk2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzQTNpPkKxOVE{Ojl|PD;hQi=>
SF268 cells M3zib2N6fG:2b4jpZ4l1gSCjc4PhfS=> NH;3bG81QCCq Mn3IR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2YzPjhiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhT0l3MDC9JFAvODR2IN88UU4> M2DmcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGzNlk1Lz5{MUWxN|I6PDxxYU6=
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HT-29 cells NFPIXIxEgXSxdH;4bYNqfHliYYPzZZk> M3;pVFQ5KGh? M3XL[mN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhVNTJ7IHPlcIx{KGGodHXyJFQ5KGi{czDifUBUWkJiYYPzZZktKEeLNUCgQUA{NjZizszNMi=> NWH3WZBFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzQTRpPkKxOVE{Ojl2PD;hQi=>
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SW1116 cells MUTGeY5kfGmxbjDhd5NigQ>? M3rYPFI1KGh? MXXJcohq[mm2aX;uJI9nKHSnbH;t[ZJie2ViaX6gbJVu[W5iU2exNVE3KGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDUVmFRNVCFUj3FUGlUSSCjc4PhfUwhUUN3MDC9JFgvOzJizszNMi=> NXXjd|ZwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5OlI2OjNpPkKxPVYzPTJ|PD;hQi=>
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ST486 cells NXXYcI5MSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MkfyOFghfG9iN{KgbC=> M3OyXWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU2S0PFYh[2WubIOgZYZ1\XJiNEigeI8hPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwMEC3JO69VS5? M1e2dFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUiyPVI6Lz5{MkG4NlkzQTxxYU6=
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MCF7 cells MkH0R5l1d3SxeHnjbZR6KGG|c3H5 MWWyOEBp NGq4WYZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFI1KGi{czD1d4lv\yCDbn7lfIlvKF[nRlnUR{9xem:yaXTpeY0hcW:maXTlJJN1[WmwaX7nJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwMUig{txONg>? NF:4bmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW0NVA2OSd-MkK1OFExPTF:L3G+
BJ cells MX;DfZRwfG:6aXPpeJkh[XO|YYm= NXW3dGVzPzJiaB?= NXjvXmN2S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSkpiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGNidGOnaX6gRW0h[XO|YYmsJGlEPTBiPTCwMlAxOiEQvF2u NUnpW5AxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5NlExQDFpPkKyPVIyODhzPD;hQi=>
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HeLa cells MlnjR5l1d3SxeHnjbZR6KGG|c3H5 NEHVSoY4OiCq MljTR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bi\nSncjC3NkBpenNiYomgR4Ft[2WrbjDBUUBie3OjeTygTWM2OCB;IECuNVc2KM7:TT6= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl{MUC4NUc,OjJ7MkGwPFE9N2F-
HepG2 cells NH;aR3lHfW6ldHnvckBie3OjeR?= NXrSWohkOjRiaB?= Mnn2TY5pcWKrdHnvckBw\iC2ZXzvcYVz[XOnIHnuJIh2dWGwIFjldGczKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDUVmFRNVCFUj3FUGlUSSCjc4PhfUwhUUN3MDC9JFQvOTRizszNMi=> NGjHZos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CxPFA6Pid-MkOwNVgxQTZ:L3G+
SW1116 cells MWrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M3TOTFQ5KGh? MlWxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVV{GxNVYh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBu\XSqb3SsJGlEPTBiPTC0Mlk2KM7:TT6= MmHBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMUiwPVYoRjJ|MEG4NFk3RC:jPh?=
HepG2 cells NIDoVmVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NEK1VYc1QCCq MlnLRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZYDHNkBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJI1mfGixZDygTWM2OCB;IE[uO|Mh|ryPLh?= MoLQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMUiwPVYoRjJ|MEG4NFk3RC:jPh?=
BGC823 cells MY\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MlTEOFghcA>? NVn0VIh2SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDCS2M5OjNiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCvZYToc4QtKEmFNUCgQUA3Njh|IN88UU4> M1qyWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEG4NFk3Lz5{M{CxPFA6PjxxYU6=
HeLa cells NX72[3RmSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MYC0PEBp NEHQeG5CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjlUIEh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBu\XSqb3SsJGlEPTBiPTC5MlEzKM7:TT6= NFTpW3A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CxPFA6Pid-MkOwNVgxQTZ:L3G+
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HepG2 cells M1K1SGZ2dmO2aX;uJIF{e2G7 M4\4R|Q5KGh? NGTMWYFKdmirYnn0bY9vKG:oIITlcI9u\XKjc3WgbY4hcHWvYX6gTIVxTzJiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHRTSVBvUFPSMWVNUVODLDDJR|UxKD1iOD6zJO69VS5? NETPWXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K3PVg3PCd-MkOyO|k5PjR:L3G+
SW1116 cells Mo\PSpVv[3Srb36gZZN{[Xl? MkDUNlQhcA>? MmLDTY5pcWKrdHnvckBw\iC2ZXzvcYVz[XOnIHnuJIh2dWGwIGPXNVEyPiClZXzsd{Bi\nSncjCyOEBpenNiYomgWHJCWC2SQ2KtSWxKW0FiYYPzZZktKEmFNUCgQUA1NjF6IN88UU4> NVXFOJBnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPFY4PjFpPkK0Nlg3PzZzPD;hQi=>
KE-97 cells M3LGOmN6fG:2b4jpZ4l1gSCjc4PhfS=> NWnNVGRnPzJiaB?= M3eyS2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtGNTl5IHPlcIx{KGGodHXyJFczKGi{czDifUBE\WyuVHn0doUuT2yxIHz1cYlv\XOlZX70JINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hOC5zMzFOwG0v M1m2e|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{K4Nlg{Lz5{NEOyPFI5OzxxYU6=
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HuH7 cells MoHBR5l1d3SxeHnjbZR6KGG|c3H5 MUC3NkBp NYDjdottS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUHWKNzDj[YxteyCjZoTldkA4OiCqcoOgZpkhS2WubGTpeJJmNUeubzDseY1qdmW|Y3XueEBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDBwMkOg{txONg>? NF3qXWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOyPFI5Oyd-MkSzNlgzQDN:L3G+
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MCF7 cells MlnPR5l1d3SxeHnjbZR6KGG|c3H5 NEnzfmY4OiCq M2\MV2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGNmdGyWaYTy[U1IdG9ibIXtbY5me2OnboSgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAxNjV{IN88UU4> NGfH[ZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOyPFI5Oyd-MkSzNlgzQDN:L3G+
NIH/3T3 cells M3LFXGN6fG:2b4jpZ4l1gSCjc4PhfS=> NXX6WWY{QTZiaB?= NF;HcoxEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBPUUhxM2SzJINmdGy|IHHmeIVzKDl4IHjyd{BjgSCVUlKgZZN{[XluIFnDOVAhRSByLkKg{txONg>? NFXLOm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2NVUzOSd-MkSzOlE2OjF:L3G+
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HT-29 cells M2Hwb2N6fG:2b4jpZ4l1gSCjc4PhfS=> NULVTYU3QTZiaB?= MYDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIWE0zQSClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyIE2gNE4zKM7:TT6= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4MUWyNUc,OjR|NkG1NlE9N2F-
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518A2 cells NVHWV2xkS3m2b4TvfIlkcXS7IHHzd4F6 M{\rXFk3KGh? NUiwZ|VwS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hPTF6QUKgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KFOUQjDhd5NigSxiSVO1NEA:KDBwMjFOwG0v MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4MUWyNUc,OjR|NkG1NlE9N2F-
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A549 cells MWjDfZRwfG:6aXPpeJkh[XO|YYm= NI[yWGk6PiCq Ml\nR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyIE2gNE43KM7:TT6= NFPhTXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2NVUzOSd-MkSzOlE2OjF:L3G+
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HEK293 cells Mnm4R5l1d3SxeHnjbZR6KGG|c3H5 M1XDd|czKGh? MULDfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{OUOgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRmekeubzDhd5NigSxiSVO1NEA:KDBwMEW2JO69VS5? NWfINVhIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OlMzPjJpPkK0O|Y{OjZ{PD;hQi=>
HeLa cells NYDGeop6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MVeyOEB1dyB2ODDo NUH3VJFYSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IEK0JJRwKDR6IHjyd{BjgSCFQ1utPEBie3OjeTygTWM2OCB;IEKuO|Ih|ryPLh?= NXPTe|l2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3PVI5OTFpPkK0O|kzQDFzPD;hQi=>
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HepG2 cells NHnKW4JEgXSxdH;4bYNqfHliYYPzZZk> M2LxcGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{NCCHQ{WwJF0hOiEQvF2u NYDOepBCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNVY{OTdpPkK1N|E3OzF5PD;hQi=>
Raji cells MmDtR5l1d3SxeHnjbZR6KGG|c3H5 MWXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDSZYpqKGOnbHzzMEBGSzVyIE2gNkDPxE1w NVnXWW5SRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNVY{OTdpPkK1N|E3OzF5PD;hQi=>
BJ cells M1vFWWN6fG:2b4jpZ4l1gSCjc4PhfS=> MXnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDCTkBk\WyuczygSWM2OCB;IEKg{txONg>? M1i0RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{G2N|E4Lz5{NUOxOlMyPzxxYU6=
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A549 cells NGiwPJFEgXSxdH;4bYNqfHliYYPzZZk> NH;VfZc1QCCq NEjlWnZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjB{IN88UU4> NGf0eng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUiyOVk{PCd-MkW4NlU6OzR:L3G+
HeLa cells MXrDfZRwfG:6aXPpeJkh[XO|YYm= NVPTTm5NPDhiaB?= M{D4PWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmVGFiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODJ3IN88UU4> NHL5NIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUiyOVk{PCd-MkW4NlU6OzR:L3G+
PC3 cells MXPDfZRwfG:6aXPpeJkh[XO|YYm= MXu0PEBp MnftR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVGM{KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlA{OSEQvF2u NWPQO3lyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4NlU6OzRpPkK1PFI2QTN2PD;hQi=>
MGC-803 cells NIrXSnBHfW6ldHnvckBie3OjeR?= MVWyOEBp MW\Jcohq[mm2aX;uJI9nKHSnbH;t[ZJie2ViaX6gbJVu[W5iTVfDMVgxOyClZXzsd{Bi\nSncjCyOEBpenNiYomgWHJCWC2SQ2KtSWxKW0FiYYPzZZktKEmFNUCgQUA5Njl5IN88UU4> MmWxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ7MEC2OVYoRjJ4OUCwOlU3RC:jPh?=
Sf9 insect cells MlWySpVv[3Srb36gZZN{[Xl? M1PNWlExKG2rboO= NIPMT2lKdmirYnn0bY9vKG:oIF6teIVzdWmwYXygS3NVKHSjZ3fl[EBz\WOxbXLpcoFvfCCodXzsMYxmdme2aDDoeY1idiCFSFuxJIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3czDT[lkhcW6|ZXP0JINmdGy|IHHmeIVzKDFyIH3pcpMh[nlibIXtbY5me2OnboSgRWRRNUeubzDhd5NigSxiSVO1NEA:KDBwMECxNkDPxE1w MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzB6OUKxNUc,OjdyOEmyNVE9N2F-
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Neuro2a cells NHLBWopEgXSxdH;4bYNqfHliYYPzZZk> NWTzUZlSPzJiaB?= MmTRR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUoV2em9{YTDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5yMUGzJO69VS5? MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDF3MkSyO{c,OjhzNUK0Nlc9N2F-
MDA-MB-231 cells M{LFRmZ2dmO2aX;uJIF{e2G7 MofmNVYhcA>? NVXZ[41SUW6qaXLpeIlwdiCxZjDy[ZRqdm:kbHHzeI9u[SCycn;0[YlvKHCqb4PwbI9zgWyjdHnvckBqdiCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEG2JIhzeyCkeTDIc4VkcHO2IEOzN|QzKHO2YXnubY5oKGKjc3XkJIZtfW:{ZYPj[Y5k\SCjc4PhfUwhUUN3MDC9JFAvODZ4IN88UU4> MmToQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2M{GzOFIoRjJ6NEOxN|QzRC:jPh?=
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BT549 cells MUfDfZRwfG:6aXPpeJkh[XO|YYm= MVeyOEBp NYC0[IpsS3m2b4TvfIlkKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gRnQ2PDliY3XscJMhcW6ldXLheIVlKG[xcjCyOEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4xQCEQvF2u MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDdyNUSzNkc,Ojh5MEW0N|I9N2F-
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アッセイ
Methods Test Index PMID
Western blot
FAK / RIP; 

PubMed: 15121855     


Western blot (WB) analysis of BT-20 and BT-474 lysates treated with staurosporine (200 nM). Control cells were treated with dimethyl sulfoxide for 18 h.

p-Akt / Akt / PARP /Cleaved PARP; 

PubMed: 24174874     


Western blot analysis for Akt, P-Akt, and cleaved poly(ADP-ribose) polymerase. U87 cells were incubated with 200 nM, 10 nM, and 0.1 nM of staurosporine encapsulated in liposomes and staurosporine for 32 hours.

Ambra1; 

PubMed: 24587252     


SW620 cells treated with various doses of staurosporine for 6 h. Ambra1 levels were measured by Western blot. 

15121855 24174874 24587252
Growth inhibition assay
Cell viability; 

PubMed: 25215174     


Staurosporine reduces cerebellar astrocytes viability. (a) Cerebellar astrocytes were treated with staurosporine 0.1 μM, 0.25 μM, and 0.5 μM for 24 h and the cell viability was measured by MTT transformation. (b) Cerebellar astrocytes were treated with staurosporine 0.5 μM for 6, 12, and 24 h and the cell viability was measured by MTT transformation. Data are presented as mean ± SEM of four independent experiments. ∗ is significantly different from control (P < 0.05).

25215174
Immunofluorescence
Phalloidin / Type II collagen; 

PubMed: 22684244     


Cell were cultured in the absence (a-f) or presence of staurosporine (STSN, 5 × 10-9M) or cytochalasin D (CD, 1 µg/ml) for 1 (upper panel) or 2 (lower panel) days at low density and stained for F-actin (Phalloidin) and type II collagen (Type II). STSN: Staurosporine.

Tubulin / Actin; 

PubMed: 25215174     


Morphological changes of astrocytes induced by St are evidenced by the rearrangement of cytoskeletal proteins. Astrocytes were treated with St (0.5 μM) for 12 hours and then were labelled with rhodamine-phalloidin or immunostained for tubulin. Representative images of phase contrast, rhodamine-phalloidin, and tubulin are shown in control and St treated astrocytes. Scale bar represents 50 μm.

FAK 4.47; 

PubMed: 15121855     


BT-20 or BT-474 cell culture were plated on six-well plates and were treated with staurosporine (200 nM) after 24 h. After 6 h of treatment, cells were immunostained with anti-FAK 4.47 antibody (white arrowheads mark some focal adhesions).

cleaved-caspase 3; 

PubMed: 19840952     


Representative images (×1000 magnification) showing vehicle treated (C) and staurosporine treated (D) cells stained with anti-cleaved caspase 3 antibody and DAPI.

Annexin; 

PubMed: 15140398     


Induction of apoptosis with staurosporine resulting in activation of the ICE-NIRF probe. Gli36 cells were treated with 50 µM staurosporine for 24 hours (A-C) or with the same percentage of DMSO (0.01%) to which experimental wells were exposed (D-488nm laser, E-633nm laser and F-bright field). To examine the role of caspase-1 in staurosporine-induced apoptosis and probe activation, cells were coincubated in caspase-1 inhibitor (10 µM) and staurosporine (G-I). Staurosporine induces apoptosis, indicated by the positive annexin staining viewed with the 488-nm laser (A), which colocalized with activated probe viewed with the 633-nm laser (B). Coincubation of the caspase-1 inhibitor with staurosporine did not completely block apoptosis, indicated by the relatively higher number of apoptotic cells stained with annexin (G), as those that activated the probe (H). Magnification, x 40; scale bar, 50 µM.

Pyk2; 

PubMed: 19880522     


Pyk2 translocates to the nucleus upon staurosporine addition to ID8 cells. Confocal immunofluorescent analysis of endogenous Pyk2 upon DMSO (control) or 1 μm staurosporine addition for 2 h. The scale bar is 10 μm. 

22684244 25215174 15121855 19840952 15140398 19880522
体内試験 In the gerbil and rat ischemia models, Staurosporine pretreatment (0.1-10 ng) before ischemia prevents neuronal damage in a dose-dependent manner, suggesting the involvement of PKC in CAl pyramidal cell death after ischemia. [8]

お薦めの試験操作(参考用のみ)

キナーゼ試験:

[1]

- 合併

Enzyme assay and binding assay:

Protein kinase C is assayed in a reaction mixture (0.25 mL) containing 5 μmol of Tris/HCl, pH 7.5, 2.5 μmol of magnesium acetate, 50 μg of histone II S, 20 μg of phosphatidylserine, 0.88 μg of diolein, 125 nmol of CaCl2, 1.25 nmol of [γ-32]ATP (5-10 × 104 cpm/nmol) and 5 μg of partially purified enzyme. The binding of [3H]PDBu to protein kinase C is determined: Reaction mixture (200 μL contained 4 μmo1 of Tris/malate, pH 6.8, 20 μmol of KCl, 30 nmol of CaC12, 20 μg of phosphatidylserine, 5 μg of partially purified protein kinase C, 0.5% (final concentration) of DMSO,10 pmol of [3H]PDBu (l-3 × 104 cpm/pmol) and 10 μL of various amounts of Staurosporine.
細胞試験:

[3]

- 合併
  • 細胞株: PC12
  • 濃度: Dissolved in DMSO, final concentration 1 μM
  • 反応時間: ~32 hours
  • 実験の流れ:

    Cells are exposed to Staurosporine for ~32 hours. Cells are fixed in 4% paraformaldehyde and stained with the DNA-binding dye Hoechst 33342. Cells are visualized under epifluorescence illumination, and the percentage of apoptotic cells (cells with condensed and fragmented DNA) is determined.


    (参考用のみ)
動物試験:

[8]

- 合併
  • 動物モデル: Male Mongolian gerbils or male Wistar rats subjected to transient ischemia
  • 投薬量: ~10 ng
  • 投与方法: Stereotaxically administered into the bilateral CAl subfield of the hippocampus
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 4 mg/mL (8.57 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。

化学情報

分子量 466.53
化学式

C28H26N4O3

CAS No. 62996-74-1
Storage powder
in solvent
別名 Antibiotic AM-2282, STS
Smiles CC12C(C(CC(O1)N3C4=CC=CC=C4C5=C6C(=C7C8=CC=CC=C8N2C7=C53)CNC6=O)NC)OC

投与溶媒組成計算器(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)
投与量 mg/kg 動物平均体重 g 投与体積(動物毎) ul 動物数
ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)
% DMSO % % Tween 80 % ddH2O
計算リセット

便利ツール

モル濃度計算器

モル濃度計算器

求めたい質量、体積または濃度を計算してください。

質量 (mg) = 濃度 (mM) x 体積 (mL) x 分子量 (g/mol)

モル濃度計算器方程式

  • 質量
    濃度
    体積
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備するために必要な希釈率を計算してください。Selleck希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 入力 出力 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

質量 濃度 体積 分子量

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

  • * 必須
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細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID