Doxorubicin (Adriamycin) HCl

Licensed by Pfizer 製品コードS1208 別名:NSC 123127

Doxorubicin (Adriamycin) HCl化学構造

分子量(MW):579.98

Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.

サイズ 価格(税別)  
In DMSO JPY 21400
JPY 16900
JPY 36800
JPY 113200
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Topoisomerase阻害剤の選択性比較

生物活性

製品説明 Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.
ターゲット
Topo II [1]
(Tumor cells)
体外試験

Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. [2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. [3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. [5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. [6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor Bortezomib, indicating that Doxorubicin may also exert its effect by damaging proteins. [8]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
L1210 NVv6fHd{TnWwY4Tpc44hSXO|YYm= MY\Jcohq[mm2aX;uJI9nKFKQQTDzfY51cGW|aYOge4l1cCCHREWwJI9nKDBwNUig{txO MWW0PVA2OzZ?
Yoshida sarcoma MWXGeY5kfGmxbjDBd5NigQ>? NGryUFFKdmirYnn0bY9vKG:oIGuzTH1V\FJiaX7jc5Jxd3KjdHnvckB4cXSqIFnDOVAhd2ZiMD6wNkDPxE1? NV7qUWh1OTN{Mkm4Oi=>
CHO MmHXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHSzNW5KSzVyPUCuNFYh|ryP MkHWNVM2PDd3MB?=
8226 M3\tb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1PzemlEPTB;MD6wN|Mh|ryP M{nTVVE1PDd5M{C=
8226/ADR MkO4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVrBW|hmUUN3ME2wMlU3KM7:TR?= M1z1fFE1PDd5M{C=
MCF7/ADR NGLUbZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVTJR|UxRTNwMTFOwG0> NGftV3AyPDR5N{Ow
H69 M17GPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoPMTWM2OD1yLki3JO69VQ>? M4TZR|E1Pzl3OEW=
N417 MoDSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVvOU3pIUUN3ME2wMlI{KM7:TR?= MYWxOFc6PTh3
H9 M{jic2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlXrTY5pcWKrdHnvckBw\iCKOTDj[YxteyClaILvcolk[WyueTDpcoZm[3SnZDD3bZRpKHSqZTDITXYuOSBqSEmvTWlKSilid3n0bEBKTDVyIH;mJFAvODFizszN M4jp[|E2PDh4OEG=
T-C8166 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXHHVXB1UUR3ME2wMlAyKM7:TR?= NEP2U5AyPTR6Nkix
T-H9 M123XGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1WxO2lFPTB;MD6wNUDPxE1? NWPNe2NEOTV2OE[4NS=>
T-MT4 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJSFUxRTBwMEGg{txO M1uwW|E2PDh4OEG=
SKMEL-5 NWXLdGFQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXTFSFUxRTNwMjFOwG0> NV24O2o{OTZzM{e1Ny=>
MLM MljsR5l1d3SxeHnjJGF{e2G7 NFu3T|JGTDVyPUCuNVMh|ryP MnfENVg4PTN3MB?=
KBM-3 NV[xNYRnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MljWTWM2OD1yLkCyOkDPxE1? MkLwNVk6PTh5Nx?=
HT-29 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYOwMlAyKM7:Zx?= M{PoN|Q5KGh? M4nadGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5 MUKyO|c5PDR7
EMT6 MnjTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXlNE42KM7:TR?= NHHEVGkyKGh? MlnITY5pcWKrdHnvckBw\iCycn;sbYZmemG2aX;uJIJ6KG2nYYP1dolv\yCob4Kgd5Vzfmm4aX7nJIZz[WO2aX;ud{B4cXSqIFXEOVAhd2ZiMD6wNFAyKM7:TR?= NFvOcGY4PDV{Nke0
H2981 MXvDfZRwfG:6aXOgRZN{[Xl? MXHJR|UxRTBwNTFOwG0> NVLwOZJwPzd|MUCyNy=>
LOX MlWwR5l1d3SxeHnjJGF{e2G7 MnO4TWM2OD1yLkC0NkDPxE1? NI\GPFc4QDV|M{Ox
C38 NWTwTlBUS3m2b4TvfIlkKEG|c3H5 NE[0TGVKSzVyPUCuNFEh|ryP NV\KfZNSQDB3N{K5NS=>
EAC NFvOVJVEgXSxdH;4bYMhSXO|YYm= MnjlO|IhcA>? NHTpfG5KSzVyPUCuN|Uh|ryP MUi4N|UxODh5
SKVLB M2\adGN6fG:2b4jpZ{BCe3OjeR?= NX\oXJN3UUN3ME21MlYh|ryP MmPWPFU4PjlzNB?=
AUC375 MmfBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYLJR|UxRTBwMUGyJO69VQ>? M33Qblg3PDh4MEC=
A2780-C25 M1XHW2N6fG:2b4jpZ{BCe3OjeR?= NFG0bJhEgXSxdH;4bYNqfHliYXfhbY5{fCCxeHHsbZBt[XSrbj3y[ZNqe3SjboSgRVI4QDBvQ{K1JJdqfGhiSVO1NEBw\iByLkC2N{DPxE1? NGi0b3U5QDNzN{W1
A2780-CP3 M2\RZ2N6fG:2b4jpZ{BCe3OjeR?= MXzDfZRwfG:6aXPpeJkh[WejaX7zeEBkcXOybHH0bY4uemW|aYP0ZY51KEF{N{iwMWNROyC5aYToJGlEPTBib3[gNE4xPTZizszN NEX1flk5QDNzN{W1
A2780-DX5 M2q3ZWN6fG:2b4jpZ{BCe3OjeR?= NX7kXHR6S3m2b4TvfIlkcXS7IHHnZYlve3RiZH;4c5J2[mmlaX6tdoV{cXO2YX70JGEzPzhyLVTYOUB4cXSqIFnDOVAhd2ZiMD6zOVch|ryP NH\lRoY5QDNzN{W1
MXF7 MYXDfZRwfG:6aXOgRZN{[Xl? NYXoS4Q5SW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5Qhe2Wwc3n0bZZmKE2[Rkege4l1cCCLQ{WwJI9nKDBwMEK4JO69VQ>? MVS4PVYxPTV6
MXF7 NEWyfGhEgXSxdH;4bYMhSXO|YYm= MX;BcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDtbZRwgGGwdILvcoUuemW|aYP0ZY51KE2[Rkege4l1cCCLQ{WwJI9nKDBwMEK4JO69VQ>? MV64PVYxPTV6
MXF7 MmHyR5l1d3SxeHnjJGF{e2G7 MofGRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4Sg[I95d3K3YnnjbY4uemW|aYP0ZY51KE2[Rkege4l1cCCLQ{WwJI9nKDFwMUeyJO69VQ>? MYS4PVYxPTV6
WiDr M3HjXWN6fG:2b4jpZ{BCe3OjeR?= NEHBR4lC[3Srdnn0fUBi\2GrboP0JJNmdnOrdHn2[UBYcUS{IHPlcIwhf2m2aDDJR|UxKG:oIECuNFU{KM7:TR?= MYO4PVYxPTV6
MCF M1;BVWZ2dmO2aX;uJGF{e2G7 MVPS[ZZmenOjbDDv[kBRNWeyLX3l[IlifGWmIH31cJRq\HK3ZzDy[ZNqe3SjbnPlJIF{e2W|c3XkJIF{KGWwaHHuZ4Vu\W62IHnuJIN6fG:2b4jpZ4l1gSCxZjDh[JJq[W27Y3nu NXjYcJdVQDl6NEG1NS=>
C6 M4raO2N6fG:2b4jpZ{BCe3OjeR?= MkPvNk42KG2pL33M Mn\mO|IhcA>? MnnpSG1UVw>? M2WzNmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KHKjdDDDOkBk\WyuczDheEA2OCC3Zz;tUEB1dyB{LkWgcYcwdUx? M13ZWlg6QDh4MES=
C6 MlTDSpVv[3Srb36gRZN{[Xl? NFXBT4kzNjVibXevcWw> MnW2OEBp M3uwd2ROW09? MYHJcohq[mm2aX;uJI9nKHS3YoXsbY4heG:ueX3ldol7[XSrb36= MUS4PVg5PjB2
C6 NHT5UYxEgXSxdH;4bYMhSXO|YYm= Mo\NNk42KG2pL33M NXzwepMzPSCq M1nFc2ROW09? NGC3[HZEgXSxdH;4bYNqfHliYYPz[ZN{\WRiYYOgZ4VtdCC{ZXzlZZNm MYC4PVg5PjB2
SKVLB NVHobWM2S3m2b4TvfIlkKEG|c3H5 NXfmbGVJPzJiaB?= NEfGNW5KSzVyPU[uOVQh|ryP MX[5NFA{PTJy
MCF7/VP16 MnLhR5l1d3SxeHnjJGF{e2G7 NGrpPVY2OCEQvHevcWw> NWDGcXI2PzJiaB?= M2ThcWlEPTB;MUSuNkDPxE1? NXu1fFBnQTB{Mke5Ni=>
CA755 MkXvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1nY[lExKM7:TR?= NWH6dG8yPiCm MkHiTWM2OD1yLkCxOEDPxE1? NEPNbIk6PTJ4NUew
CHO M4nrcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjhbGJzOTBizszN NH7Td2Q3KGR? NWTiblZkUUN3ME2wMlA3KM7:TR?= MWi5OVI3PTdy
Panc02 M{juXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWexNEDPxE1? NYWyTFVVPiCm M2DV[WlEPTB;MD6wOlUh|ryP NELl[486PTJ4NUew
PC14 NFHzb2FEgXSxdH;4bYMhSXO|YYm= NYrNdHZQPCCm M2DTTGROW09? NIDkPXpKSzVyPUCuNlkh|ryP MkfFPVgzOjV2Mh?=
HOP62 NW\ofItLS3m2b4TvfIlkKEG|c3H5 MXi3NkBp NETkZXBKSzVyPUCuNFA1KM7:TR?= M2r0S|k5PzN4NkG=
SF-295 MlPKR5l1d3SxeHnjJGF{e2G7 NGjJUYM4OiCq NVnVcJhYUUN3ME2wMlA{PSEQvF2= M3GyOlk5PzN4NkG=
MDA-MB-361 M1[1XmN6fG:2b4jpZ{BCe3OjeR?= NYfJPJNkOSCq MWHEUXNQ M3TJU2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GQT3NRk0{PjFiZYjwdoV{e2mwZzDMZYNbKHerdHigTWM2OCCxZjCwMlAyOiEQvF2= NUHDXoFoOTB|OUW0PVA>
MDA-MB-361 MYDDfZRwfG:6aXOgRZN{[Xl? NGLiT2YyKGh? NVrCOmtOTE2VTx?= NX\w[HkyS3m2b4TvfIlkcXS7IHHnZYlve3RiTVTBJG1DKDN4MTDlfJBz\XO|aX7nJINiemKxeInw[ZB1cWSjc3Wgd5REWEd{IIfpeIghUUN3MDDv[kAxNjBzODFOwG0> NGHXe4wyODN7NUS5NC=>
CCRF-SB MoTZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHj[oo1KGR? NX\hOVZSTE2VTx?= NFrWN49KSzVyPUCuNFEh|ryP M2Hn[lExPDFzNEe2
CHO-K1 NIPHWJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlfpOEBl NUS2VXI1TE2VTx?= MlXqTWM2OD1yLkSg{txO NF\qeoQyODRzMUS3Oi=>
KB MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYq0JIQ> MX3EUXNQ NX3wXlg{SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBlenWpLYLld4l{fGGwdDDLRldFKHerdHigTWM2OCCxZjCwMlY2KM7:TR?= NGnHNpIyODRzMUS3Oi=>
KB MnLCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUPRZpV5PCCm MnnSSG1UVw>? NVrO[Ic1SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBlenWpLYLld4l{fGGwdDDLRmNidXC5aYToJGlEPTBib3[gNE4xPiEQvF2= M1zHPVExPDFzNEe2
KB NUTYR3ZMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUHNcXVUPCCm M4fSd2ROW09? MYfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTyeYcuemW|aYP0ZY51KEuETVTSJJdqfGhiSVO1NEBw\iByLkWg{txO Mm[3NVA1OTF2N{[=
KB M4\wVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NInWdnQ1KGR? M{XoWGROW09? NF\NeHJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIRzfWdvcnXzbZN1[W62IFvCWlIxSyC5aYToJGlEPTBib3[gNE41KM7:TR?= NFGzVJIyODRzMUS3Oi=>
HTB-54 NH62cHpEgXSxdH;4bYMhSXO|YYm= MlS1NVAxKM7:TR?= NXPoSG5QUUN3ME2xMlEh|ryP Moe0NVA1PzZ6NkG=
MEL-AC NGnaVmhEgXSxdH;4bYMhSXO|YYm= M{PkdVExOCEQvF2= NU\xcodwUUN3ME2xMlIh|ryP NYDUZ3E2OTB2N{[4OlE>
DU145 MWrDfZRwfG:6aXOgRZN{[Xl? NVzjSox4PzJiaB?= NFH3d3RKSzVyPUCuNFUzQCEQvF2= NYLsTWpuOTB2N{myPFI>
K562R NF7GNo1EgXSxdH;4bYMhSXO|YYm= NF73dJQ4OiCq MYfJR|UxRTBwMEG1OkDPxE1? NVXCN4I5OTB2N{myPFI>
PACA-2 NVfDbWtmS3m2b4TvfIlkKEG|c3H5 NUHUfnFvPiCm NUjjVIdYTUR3ME2wMlAxPjNizszN M2nLSlExPDl6MkG0
3Y1 MUTDfZRwfG:6aXOgRZN{[Xl? MVu3NkBp MXzHTVUxRTBwMECzPEDPxE1? NFjhPHIyODZ7MUexNC=>
P388 NWrWdIl7S3m2b4TvfIlkKEG|c3H5 M2jE[|czKGh? NFP4VWFIUTVyPUCuNFEzKM7:TR?= MoH3NVA3QTF5MUC=
HMV-1 M4\rS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYPpfGJ5UUN3ME2wMlI5KM7:TR?= NHzycXEyODZ7OESzOi=>
MKN-1 M4DVWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTBwNkSg{txO NVfweFc{OTB4OUi0N|Y>
PC14 Mnf2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1HzR2lEPTB;MT65JO69VQ>? MX2xNFY6QDR|Nh?=
T24 NEjEfXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYjIUGI5UUN3ME2wMlY1KM7:TR?= NF;pNJQyODZ7OESzOi=>
U937 M1j1OWZ2dmO2aX;uJGF{e2G7 M2CyV|I1KGh? Mk\xSG1UVw>? NHfCO|hKdmirYnn0d{BqdmOxcoDvdoF1cW:wIH;mJHs{UF22aIntbYRqdmVid3n0bEBKSzVyIH;mJFAvOSEQvF2= M{jaPFExPzhyOUGx
H460pv8/eto NVfqVnNrS3m2b4TvfIlkKEG|c3H5 NFLJdlYyODBizszN MXu0PEBp MUPTZYxqdmV? NELXTJBKSzVyPUCuNFIh|ryP MUGxNFc5ODlzMx?=
H460pv8 NFG3S3dEgXSxdH;4bYMhSXO|YYm= NULte402OTByIN88US=> NFHu[|I1QCCq M4PBOnNidGmwZR?= MXTJR|UxRTBwMEC0JO69VQ>? M17kVFExPzhyOUGz
MDA-231 NVPSemZmS3m2b4TvfIlkKEG|c3H5 M37Y[VExOCEQvF2= MoDoOFghcA>? MUnTZYxqdmV? M4n6bWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GQT2yN|Eh[2WubDCoSXIuNEWJRmKrMI12fGGwdDDwOVMqKHerdHigTWM2OCCxZjCwMlAxPiEQvF2= MYWxNFc5ODlzMx?=
MDA-468 NHG4THJEgXSxdH;4bYMhSXO|YYm= NGK0[lEyODBizszN NV3RfVJFPDhiaB?= MYPTZYxqdmV? M13RemN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GQT20Olgh[2WubDCoSXIuNCCjbYDsbYZq\WRiRVfGVkwhdXW2YX70JJA2Oylid3n0bEBKSzVyIH;mJFAvODB3IN88US=> NH\hOnkyODd6MEmxNy=>
NCI-H226 NUnD[FVyS3m2b4TvfIlkKEG|c3H5 MofMNVAxKM7:TR?= NGDEVXU1QCCq MXXTZYxqdmV? Ml;RR5l1d3SxeHnjbZR6KGGpYXnud5QhVkOLLViyNlYh[2WubDCocZV1[W62IIC1N{khf2m2aDDJR|UxKG:oIECuNFA5KM7:TR?= MV:xNFc5ODlzMx?=
NCI-H322 MWXDfZRwfG:6aXOgRZN{[Xl? M4qwW|ExOCEQvF2= MoDZOFghcA>? NUm0eFFrW2GuaX7l NX\ZPJN5S3m2b4TvfIlkcXS7IHHnZYlve3RiTlPJMWg{OjJiY3XscEApdXW2YX70JJA2Oylid3n0bEBKSzVyIH;mJFAvODNizszN MUSxNFc5ODlzMx?=
NCI-H358 MWnDfZRwfG:6aXOgRZN{[Xl? NYCwWphFOTByIN88US=> NFzWbJU1QCCq M3P5XXNidGmwZR?= NHvYeG9EgXSxdH;4bYNqfHliYXfhbY5{fCCQQ1mtTFM2QCClZXzsJEhufXSjboSgdFU{MSC5aYToJGlEPTBib3[gNE4yOyEQvF2= Mni1NVA4QDB7MUO=
NCI-H647 MVfDfZRwfG:6aXOgRZN{[Xl? MnvJNVAxKM7:TR?= MlLvOFghcA>? NUG3VJE5W2GuaX7l NEfSdndEgXSxdH;4bYNqfHliYXfhbY5{fCCQQ1mtTFY1PyClZXzsJEhufXSjboSgdFU{MSC5aYToJGlEPTBib3[gNE4xODFizszN M2LYR|ExPzhyOUGz
SKBR-3 MXzDfZRwfG:6aXOgRZN{[Xl? MUexNFAh|ryP NH\4b3Y1QCCq NH\vNFBU[WyrbnW= NGjub2tEgXSxdH;4bYNqfHliYXfhbY5{fCCVS1LSMVMh[2WubDCoSXIu[W2ybHnmbYVlKGW{QkKscZV1[W62IIC1N{khf2m2aDDJR|UxKG:oIECuNFIh|ryP M3jFWlExPzhyOUGz
T47D MmjoR5l1d3SxeHnjJGF{e2G7 MnnlNVAxKM7:TR?= NGXBd2Y1QCCq NXO0UmNFW2GuaX7l NIDVPGpEgXSxdH;4bYNqfHliYXfhbY5{fCCWNEfEJINmdGxiKFXSL{xufXSjboSgdFU{MSC5aYToJGlEPTBib3[gNE4xODdizszN MVqxNFc5ODlzMx?=
ZR-75-1 NYC3ZZFwS3m2b4TvfIlkKEG|c3H5 MlvsNVAxKM7:TR?= MXm0PEBp NIj2PWFU[WyrbnW= MoHWR5l1d3SxeHnjbZR6KGGpYXnud5QhYlJvN{WtNUBk\WyuIDjFVksteGe{KzzteZRidnRicEWzLUB4cXSqIFnDOVAhd2ZiMD6wNFkh|ryP NUjjU3pMOTB5OEC5NVM>
L1210 NIXpd21EgXSxdH;4bYMhSXO|YYm= NGDle5hKSzVyPUCuNFI2KM7:TR?= M1[yUVExQDlyMU[3
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