Cytarabine (U-19920A)

製品コード:S1648 別名:Cytarabin, Ara-C, Arabinofuranosyl Cytidine, Cytosine β-D-arabinofuranoside, Cytosine arabinoside, NSC 63878, NSC 287459

For research use only.

Cytarabine (U-19920A, Cytarabin, Ara-C, Arabinofuranosyl Cytidine, Cytosine β-D-arabinofuranoside, Cytosine arabinoside, NSC 63878, NSC 287459) is an antimetabolic agent and DNA synthesis inhibitor with IC50 of 16 nM in wild-type CCRF-CEM cells. Cytarabine induces autophagy and apoptosis.

Cytarabine (U-19920A)化学構造

CAS No. 147-94-4

サイズ 価格(税別)
10mM (1mL in DMSO) JPY 15900
JPY 18100
JPY 40880

代表番号: 03-5615-9297|電子メール:[email protected]
よく尋ねられる質問

文献中Selleckの製品使用例(64)

製品安全説明書

DNA/RNA Synthesis阻害剤の選択性比較

相関DNA/RNA Synthesis製品

生物活性

製品説明 Cytarabine (U-19920A, Cytarabin, Ara-C, Arabinofuranosyl Cytidine, Cytosine β-D-arabinofuranoside, Cytosine arabinoside, NSC 63878, NSC 287459) is an antimetabolic agent and DNA synthesis inhibitor with IC50 of 16 nM in wild-type CCRF-CEM cells. Cytarabine induces autophagy and apoptosis.
特性 The 1st of a series of cancer drugs that alters the sugar component of nucleosides.
ターゲット
DNA synthesis [1]
(CCRF-CEM cells)
16 nM
体外試験

Cytarabine (AraC) is phosphorylated into a triphosphate form (Ara-CTP) involving deoxycytidine kinase (dCK), which competes with dCTP for incorporation into DNA, and then blocks DNA synthesis by inhibiting the function of DNA and RNA polymerases. Cytarabine displays a higher growth inhibitory activity towards wild-type CCRF-CEM cells compared to other acute myelogenous leukemia (AML) cells with IC50 of 16 nM. [1] Increasing concentrations of Cytarabine (IC50 of 0.69 μM) results in decreased metabolic activity of sensitive rat leukemic cell line RO/1, and the cell toxity can be highly enhanced by transfection with human wt dCK (IC50 of 0.037 μM) but not the inactive, alternatively spliced dCK forms. [2] Cytarabine apparently induces apoptosis of rat sympathetic neurons at 10 μM, of which 100 μM shows the highest toxicity and kills over 80% of the neurons by 84 hours, involving the release of mitochondrial cytochrome-c and the activation of caspase-3, and the toxicity can be attenuated by p53 knockdown and delayed by bax deletion. [3]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
murine leukemia P388 MmfpSpVv[3Srb36gZZN{[Xl? NHnhSotCdnSrY3HuZ4VzKGGldHn2bZR6KGOjcoLp[YQhd3W2IHnuJJZqfHKxIHHnZYlve3RibYXybY5mKGyndXvlcYliKFB|OEisJGlEPTB;MD6xOEDPxE1? NWr2SGo{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUGzOVYyOTBpPkGxN|U3OTFyPD;hQi=>
L1210 cell lines MoXBSpVv[3Srb36gZZN{[Xl? NVzXbXhQS2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmirYnn0JJJmeGyrY3H0bY9vKG:oIFyxNlExKGOnbHygcIlv\XNiYomgOVAmKGGodHXyJFczKGi{IHnuZ5Vj[XSrb36sJGVFPTB;MD6wOUDPxE1w M2C4N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NUKwNlQoRjF4NUKwNlQ9N2F-
CCRF-CEM cell lines M1LFU2Z2dmO2aX;uJIF{e2G7 NUDxZZFqPzJiaB?= M{XOSWNwdmOnboTyZZRqd25icnXxeYlz\WRidH:gbY5pcWKrdDDy[ZBtcWOjdHnvckBw\iCFQ2LGMWNGVSClZXzsJIxqdmW|IHL5JFUxLSCjZoTldkA4OiCqcjDpcoN2[mG2aX;uMEBGTDVyPU[gcm0v NH7BZmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkWyNFI1Lz5zNkWyNFI1RC:jPh?=
CCRF-CEM cell line MmnXSpVv[3Srb36gZZN{[Xl? NVXlWmZqUW6qaXLpeEB1cGVicnXwcIlk[XSrb36gc4YhS0OURj3DSW0h[2WubDDsbY5mKGmwII\peJJwNCCHREWwQVUhdk1w NXLaZnJkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5OVg6PCd-MUm5OVg6PDxxYU6=
HeLa human cell lines M2DzVWZ2dmO2aX;uJIF{e2G7 NYTGelJsOTR2IHi= M4H3T3Rp\SClb33wc5Vv\CC5YYOgeIV{fGWmIHnuJJZqfHKxIH\vdkBidnSrdIXtc5Ih[WO2aY\peJkpPTBnIHnubIljcXSrb36gc4YhfHWvb4KgZ4VtdCCpcn;3eIgqKGGpYXnud5QhUGWOYTDoeY1idiClZXzsJIxqdmW|IHHmeIVzKDF2NDDodkwhUUR3ME2wMlEh|ryP Mki0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzZ{NUexOEc,OzZ{NUexOFww[T5?
L1210 murine cell lines NFPmOYVHfW6ldHnvckBie3OjeR?= M1vmc|czKGh? M{X1bXRp\SClb33wc5Vv\CC5YYOgeIV{fGWmIHnuJJZqfHKxIH\vdkBidnSrdIXtc5Ih[WO2aY\peJkpPTBnIHnubIljcXSrb36gc4YhfHWvb4KgZ4VtdCCpcn;3eIgqKGGpYXnud5QhfGinIFyxNlExKG23cnnu[UBk\WyuIHzpcoV{KGGodHXyJFczKGixdYLzMEBKTDVyPUKwJI5ONg>? M4DjOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzN4MkW3NVQoRjN4MkW3NVQ9N2F-
P388 murine cell lines MYjGeY5kfGmxbjDhd5NigQ>? MWS3NkBp M1HHeXRp\SClb33wc5Vv\CC5YYOgeIV{fGWmIHnuJJZqfHKxIH\vdkBidnSrdIXtc5Ih[WO2aY\peJkpPTBnIHnubIljcXSrb36gc4YhfHWvb4KgZ4VtdCCpcn;3eIgqKGGpYXnud5QhfGinIGCzPFghdXW{aX7lJINmdGxibHnu[ZMh[W[2ZYKgO|IhcG:3coOsJGlFPTB;MkCgcm0v NYjkbWtFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{[yOVcyPCd-M{[yOVcyPDxxYU6=
RPMI-6410 human leukemic lymphoblastoid cell lines MVnDfZRwfG:6aXRCpIF{e2G7 MnTDR5l1d3SxeHnjbZR6KGGpYXnud5QhWlCPST22OFExKGi3bXHuJIxmfWunbXnjJIx6dXCqb3LsZZN1d2mmIHPlcIwhdGmwZYOsJGlEPTB;MD6wOkDPxE1? M2PBTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzZ6Mke1OFYoRjZ6Mke1OFY9N2F-
CCRF-CEM human leukemic lymphoblastoid cell lines MWHDfZRwfG:6aXRCpIF{e2G7 MmDmR5l1d3SxeHnjbZR6KGGpYXnud5QhS0OURj3DSW0hcHWvYX6gcIV2c2WvaXOgcJlueGixYnzhd5RwcWRiY3XscEBtcW6nczygTWM2OD1{MDDuUU4> M3yyeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzZ6Mke1OFYoRjZ6Mke1OFY9N2F-
MOLT-4 human leukemic lymphoblastoid cell lines MmTvR5l1d3SxeHnjxsBie3OjeR?= M{nCWGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2RTGStOEBpfW2jbjDs[ZVs\W2rYzDsfY1xcG:kbHHzeI9q\CClZXzsJIxqdmW|LDDJR|UxRTFyIH7NMi=> MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek83QDJ5NUS2K|43QDJ5NUS2QE9iRg>?
human leukemia cell line(CCRF-CEM) NHLFfoxEgXSxdH;4bYPDqGG|c3H5 NVTDXoNIUW5idnn0do8h[3m2b4TvfIlkcXS7IH;mJINwdXCxdX7kd{B4\XKnIHTleIVzdWmwZXSgc44hUW6qaXLpeIlwdiCxZjDoeY1idiCuZYXr[Y1q[SClZXzsJIxqdmVqQ1PSSk1ETU1rLDDJR|UxRTBwMEmg{txONg>? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek85QTF5NkS1K|45QTF5NkS1QE9iRg>?
HL60 NXz4U4pGTnWwY4Tpc44h[XO|YYm= Mo\BTY4hfmm2cn:gZ49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDpcoR2[2ViYYDvdJRwe2m|IHnuJGhNPjBiY3XscJMtKEGFNUCgQUAxNjF3IN88UU4> MmDFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ6N{e1PVMoRjF{OEe3OVk{RC:jPh?=
HL60R MmPtSpVv[3Srb36gZZN{[Xl? M4\6W2lvKH[rdILvJINwdmOnboTyZZRqd25icnXxeYlz\WRidH:gbY5lfWOnIHHwc5B1d3OrczDpckBJVDZyUjDj[YxteyxiQVO1NEA:KDhizszNMi=> MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjh5N{W5N{c,OTJ6N{e1PVM9N2F-
CCRF-CEM MVvDfZRwfG:6aXPpeJkh[XO|YYm= MkLqOFghcHK| M{LnWGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNEWkZvQ1XNJINmdGy|IHHmeIVzKDR6IHjyd{BjgSClZXzsJJRqfGW{LXLseYUh[XO|YYmsJGVEPTBiPTCwMlAxPTZzNDFOwG0v M{PwNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{SzPFU5Lz5zOUe0N|g2QDxxYU6=
CCRF-CEM/C2 M17iW2N6fG:2b4jpZ4l1gSCjc4PhfS=> MnTNOFghcHK| MYLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDDR3JHNUOHTT;DNkBk\WyuczDh[pRmeiB2ODDodpMh[nliY3XscEB1cXSncj3icJVmKGG|c3H5MEBGSzVyIE2gNE4xOjJ6IN88UU4> NELicIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUe0N|g2QCd-MUm3OFM5PTh:L3G+
HL60/MX2 MWnDfZRwfG:6aXPpeJkh[XO|YYm= M2XFSVQ5KGi{cx?= NYfPTnhlS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEx4MD;NXFIh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IHPlcIwhfGm2ZYKtZox2\SCjc4PhfUwhTUN3MDC9JFAvOjZzMjFOwG0v M3vEO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{SzPFU5Lz5zOUe0N|g2QDxxYU6=
HL60 M1:xeWN6fG:2b4jpZ4l1gSCjc4PhfS=> MUG0PEBpenN? M4nX[mN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMh[W[2ZYKgOFghcHK|IHL5JINmdGxidHn0[ZIu[my3ZTDhd5NigSxiRVO1NEA:KDBwNkW3NkDPxE1w M4TkeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{SzPFU5Lz5zOUe0N|g2QDxxYU6=
L1210 M1LTU2N6fG:2b4jpZ4l1gSCjc4PhfS=> M4\6VFczKGi{cx?= MXTDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDMNVIyOCClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCvZXHzeZJm\CCjdDC3NkBpenNiYomgeJJ6eGGwIHLseYUh\XilbIXzbY9vKGG|c3H5MEBGTDVyIE2gNE4yKM7:TT6= M37rUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzOEK1PEc,OTF6MkW4QE9iRg>?
BGM NE[1dYZCdnSrdnnyZYwh[XO|YYm= MXS1JIRigXN? MWfBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDWZYNkcW6rYTD2bZJ2eyCLSFSgbY5n\WO2ZXSgbY4hSW[{aXPhckBoemWnbjDtc45s\XliQlfNJINmdGy|IHHzd4V{e2WmIHHzJJBt[XG3ZTDy[YR2[3Srb36gcYVie3W{ZXSgc44h\GG7IEWgZpkh\Gm|azDkbYZnfXOrb36gZZN{[XluIFXEOVAhRSByLkC3JO69VS5? NE\LbZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{i4O|MoRjF5OEi3N|ww[T5?
BGM NVHvR5NWSW62aY\pdoFtKGG|c3H5 MkXkOUBl[Xm| NYfIbZl3SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUGW{cHXzJHNqdXCuZYigeolzfXNiSF[gbY5n\WO2ZXSgbY4hSW[{aXPhckBoemWnbjDtc45s\XliQlfNJINmdGy|IHHzd4V{e2WmIHHzJJBt[XG3ZTDy[YR2[3Srb36gcYVie3W{ZXSgc44h\GG7IEWgZpkh\Gm|azDkbYZnfXOrb36gZZN{[XluIFXEOVAhRSByLkG2JO69VS5? MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh6N{OnQlE4QDh5M{yvZV4>
L1210 NYfJeWtDTnWwY4Tpc44h[XO|YYm= MWnJcohq[mm2aX;uJI9nKHSqeX3p[Jlt[XSnIIP5cpRp[XOnIHnuJGwyOjFyIHPlcIx{NCCHREWwJF0hOC5yMzFOwG0v MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ5M{K5K|4{ODJ5M{K5QE9iRg>?
BALL-1 M{PLWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NETPcXAzPCCqcoO= NVrGeWtKT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hSkGOTD2xJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCZU2StNUBie3OjeTygS2k2OCB;IECuNFgh|ryPLh?= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ4NEG3NEc,OjJ{NkSxO|A9N2F-
HCT116 MnXKS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVSyOEBpenN? MV3Hdo94fGhiaX7obYJqfGmxbjDv[kBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{Bi\nSncjCyOEBpenNiYomgW3NVNTFiYYPzZZktKEeLNUCgQUAxNjF{IN88UU4> M372V|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mk[0NVcxLz5{MkK2OFE4ODxxYU6=
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A549 MXHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoDaNlQhcHK| NHjGZZVIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFI1KGi{czDifUBYW1RvMTDhd5NigSxiR1m1NEA:KDBwMjFOwG0v NUjUeZNDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOlQyPzBpPkKyNlY1OTdyPD;hQi=>
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NUGC3 NY\UZW5zT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NX:1V2xkOjRiaILz NGD0dJRIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBPXUeFMzDj[YxteyCjZoTldkAzPCCqcoOgZpkhX1OWLUGgZZN{[XluIFfJOVAhRSByLkK1JO69VS5? MnG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{NkSxO|AoRjJ{Mk[0NVcxRC:jPh?=
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leukemia L1210 NELHfFBHfW6ldHnvckBie3OjeR?= MoPWTY5pcWKrdHnvckBw\iCOMUKxNEBtgW2yaH;p[EBt\XWtZX3pZUBk\WyuczC0PEBpeiCjZoTldkBi\G2rbnnzeJJifGmxbjCocIVv\3SqIH;mJJBz\WmwY4XiZZRqd25;MDDodkktKEmFNUCgQUAxNjBzIN88UU4> MoPFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV{N{e4N{c,OTV{N{e4N|ww[T5?
leukemia L1210 M4LPTWZ2dmO2aX;uJIF{e2G7 NHXrWoZKdmirYnn0bY9vKG:oIFyxNlExKGy7bYDoc4llKGyndXvlcYliKGOnbHzzJFczKGi{IHHmeIVzKGGmbXnubZN1emG2aX;uJEht\W6pdHigc4YheHKnaX7jeYJifGmxbk2wJIhzMSxiSVO1NEA:KDBwMEGg{txONg>? NFfTNW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUK3O|g{Lz5zNUK3O|g{RC:jPh?=
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thymidine kinase deficient 143B NGC2S3VEgXSxdH;4bYNqfHliYYPzZZk> M2\qUlczKGi{cx?= NIK0XVREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckB1cHmvaXTpcoUhc2mwYYPlJIRm\mmlaXXueEAyPDOEIHPlcIx{KGGodHXyJFczKGi{czDifUBUWkJiYYPzZZktKEmFNUCgQUAyPi56ODFOwG0v MoL2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyN{OwOVUoRjJ5MEezNFU2RC:jPh?=
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SNU638 NIfi[|FEgXSxdH;4bYNqfHliYYPzZZk> MVm3NkBpenN? M4rZVGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNPXTZ|ODDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG|ZTDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB5MjDodpMh[nliU2LCJIF{e2G7LDDJR|UxKD1iMT6wPEDPxE1w MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTlyNk[4O{c,Ojl7ME[2PFc9N2F-
A549 NHTocohEgXSxdH;4bYNqfHliYYPzZZk> M3nZR|czKGi{cx?= NEn5UJNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDd{IHjyd{BjgSCVUlKgZZN{[XluIFnDOVAhRSBzLke1JO69VS5? M1zPRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OUC2Olg4Lz5{OUmwOlY5PzxxYU6=
SKHEP1 MWLDfZRwfG:6aXPpeJkh[XO|YYm= NUH0TI9VPzJiaILz MV7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT2hGWDFiY3XscJMh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KFOUQjDhd5NigSxiSVO1NEA:KDFwODFOwG0v NHfvemk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUmwOlY5Pyd-Mkm5NFY3QDd:L3G+
HCT116 M3XLcmN6fG:2b4jpZ4l1gSCjc4PhfS=> NF\3RW44OiCqcoO= MUjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KFOUQjDhd5NigSxiSVO1NEA:KDRwNDFOwG0v MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTlyNk[4O{c,Ojl7ME[2PFc9N2F-
MDA-MB-231 Mn:4R5l1d3SxeHnjbZR6KGG|c3H5 MWm3NkBpenN? MVTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKGOnbHygeoli[mmuaYT5JIFnfGW{IEeyJIhzeyCkeTDTVmIh[XO|YYmsJGlEPTBiPTCxNE41QSEQvF2u MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTlyNk[4O{c,Ojl7ME[2PFc9N2F-
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Jurkat E6-1 MmTzRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M3vke2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSoXyb4F1KEV4LUGgZ4VtdHNuIFnDOVAhRSByLkCxPEDPxE1w M2X3fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMUG0OlYyLz5|MEGxOFY3OTxxYU6=
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アッセイ
Methods Test Index PMID
Western blot p-mTOR / mTOR / p-S6K / S6K / p-AMPK / AMPK / p-AKT / AKT / p-ERK / ERK 24714637
Growth inhibition assay Cell viability 24714637
体内試験 Cytarabine is highly effective against acute leukaemias, which causes the characteristic G1/S blockage and synchronization, and increases the survival time for leukaemic Brown Norway rats in a weak dose-related fashion indicating that the use of higher dosages of Cytarabine does not contribute to its antileukaemic effectiveness in man. [4] Cytarabine (250 mg/kg) also causes placental growth retardation and increases placental trophoblastic cells apoptosis in the placental labyrinth zone of the pregnant Slc:Wistar rats, which increases from 3 hour after the treatment and peaks at 6 hour before returning to control levels at 48 hour, with remarkably enhanced p53 protein, p53 trancriptional target genes such as p21, cyclinG1 and fas and caspase-3 activity. [5]

お薦めの試験操作(参考用のみ)

キナーゼ試験:[1]
  • In Vitro Growth Inhibition Assay:

    Stock solution of Cytarabine is prepared in absolute ethanol, and serial dilutions of Cytarabine are prepared. CCRF-CEM cells are suspended in RPMI medium supplemented with 10% FBS, 0.1% gentamicin, and 1% sodium pyruvate. The cells are suspended in their respective media to give 10 mL volumes of cell suspension at a final density of 3-6 × 104 cells/mL. Appropriate volumes of Cytarabine solution are transferred to the cell suspensions, and incubation is continued for 72 hours. The cells are spun down and resuspended in fresh Cytarabine -free medium, and final cell counts are determined. The data are analyzed by sigmoidal curve fitting of the cell count versus Cytarabine concentration, and the results are expressed as the IC50 (Cytarabine concentration that inhibits cell growth to 50% of the control value).

細胞試験:[2]
  • 細胞株:Rat leukemic cell lines RCL/0, RO/1 and K7 and human myelomonocytic leukemic U937
  • 濃度: ~100 μM
  • 反応時間:24, 48 and 72 hours
  • 実験の流れ:Cells are incubated in the presence of different concentrations of Cytarabine at 37 °C for 24, 48, and 72 hours. At the time of 20-, 44-, or 68-hour incubation in the presence of Cytarabine, 10 mL of cell proliferation reagent WST-1 solution is added. After 2- and 4-hour incubation with WST-1, cell metabolic activity is assessed with colorimetric changes quantified by measuring the absorbance in a spectrophotometer at 450 nm. And cell division times are calculated from eosin counting in parallel with viability assay
動物試験:[4]
  • 動物モデル:Brown Norway rat with myelocytic leukaemia
  • 投薬量:5 - 1000 mg/kg
  • 投与方法:Injection i.v.

溶解度 (25°C)

体外

体内

左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
Saline
混合させたのち直ちに使用することを推奨します。

30 mg/mL

化学情報

分子量 243.22
化学式

C9H13N3O5

CAS No. 147-94-4
Storage 3年 -20°C
2年 -80°C in solvent
Smiles C1=CN(C(=O)N=C1N)C2C(C(C(O2)CO)O)O

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

便利ツール

質量 濃度 体積 分子量

臨床試験

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05319587 Not yet recruiting Drug: Liposomal Annamycin|Drug: Cytarabine Leukemia Myeloid Acute Moleculin Biotech Inc. July 31 2022 Phase 1|Phase 2
NCT05372536 Recruiting Other: parent-led home-administered low-dose Cytarabine Supportive Care|Childhood Cancer Rigshospitalet Denmark March 11 2022 Not Applicable
NCT05215639 Recruiting -- Acute Myeloid Leukemia AbbVie December 27 2021 --

(data from https://clinicaltrials.gov, updated on 2022-08-01)

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

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Tags: Cytarabine (U-19920A)を買う | Cytarabine (U-19920A) ic50 | Cytarabine (U-19920A)供給者 | Cytarabine (U-19920A)を購入する | Cytarabine (U-19920A)費用 | Cytarabine (U-19920A)生産者 | オーダーCytarabine (U-19920A) | Cytarabine (U-19920A)化学構造 | Cytarabine (U-19920A)分子量 | Cytarabine (U-19920A)代理店