Fludarabine (NSC 118218)

For research use only. Not for use in humans.

製品コードS1491 別名:FaraA, Fludarabinum

Fludarabine (NSC 118218)化学構造

CAS No. 21679-14-1

Fludarabine (NSC 118218, FaraA, Fludarabinum) is a STAT1 activation inhibitor which causes a specific depletion of STAT1 protein (and mRNA) but not of other STATs. Also a DNA synthesis inhibitor in vascular smooth muscle cells. Fludarabine induces apoptosis.

サイズ 価格(税別)  
10mM (1mL in DMSO) JPY 22900
JPY 18100
JPY 53400
JPY 130160
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バルク問合せ

文献中Selleckの製品使用例(103)

製品安全説明書

STAT阻害剤の選択性比較

生物活性

製品説明 Fludarabine (NSC 118218, FaraA, Fludarabinum) is a STAT1 activation inhibitor which causes a specific depletion of STAT1 protein (and mRNA) but not of other STATs. Also a DNA synthesis inhibitor in vascular smooth muscle cells. Fludarabine induces apoptosis.
ターゲット
STAT1 [4]
(Vascular smooth muscle cells)
体外試験

Fludarabine efficiently inhibits the proliferation of RPMI 8226 cells with IC50 of 1.54 μg/mL. The IC50 of Fludarabine against MM.1S and MM.1R cells is 13.48 μg/mL and 33.79 μg/mL, respectively. In contrast, U266 cells are resistant to Fludarabine with IC50 of 222.2 μg/mL. Fludarabine treatment results in increased number of cells in the G1 phase of cell cycle, accompanied with a concomitant reduction of cells at the S phase of cell cycle in a time-dependent manner. Fludarabine induces a cell cycle block and triggers apoptosis in MM cells. Fludarabine triggers time-dependent cleavage of caspase-8, -9, and -3, -7, followed by PARP cleavage. Fludarabine increases expression of Bax in a time-dependent fashion, while the expression of Bak doesn't change. After exposure to Fludarabine for 12 hours, RPMI 8226 cells shows a loss of membrane potential with 61.05% of the cells expressing low fluorescence of rhodamine 123 compared with 8.62% of cells in untreated control. [1] To enhance solubility, Fludarabine is formulated as the monophosphate (F-ara-AMP, fudarabine), which is instantaneously and quantitatively dephosphorylated to the parent nucleoside upon intravenous infusion. Inside the cells rephosphorylation occurs which leads to fuoroadenine arabinoside triphosphate (F-ara-ATP), the major cytotoxic metabolite of F-ara-A. [2] Fludarabine can also induce pro-inflammatory stimulation of monocytic cells, as evaluated by increased expression of ICAM-1 and IL-8 release. [3] Fludarabine does not affect the growth of ovarian cancer cell lines, whereas it induces marked and dose-dependent inhibition of proliferation in melanoma cell lines. [4] Fludarabine induces significant reduction of STAT-1 phosphorylation, whereas it does not change JAK2 activation. Interestingly, Fludarabine does not significantly affect the phosphorylation of these three STAT proteins. Fludarabine (1.5 mg) significantly prevents STAT-1 phosphorylation and also reduces the increased amount of this protein. No significant changes are demonstrated in JAK2 phosphorylation at 2 days, but Fludarabine inhibits JAK2-increased expression at 7 days. Fludarabine specifically inhibits STAT-1 activation without affecting other STAT proteins and consequently diminishes VSMC proliferation. [5]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Jeko-1  NGDBdHRHfW6ldHnvckBCe3OjeR?= NHzEd4UzOCEQvF2= NWLzUGE1OjRiaB?= NVPzWVhvcW6qaXLpeJMh\XiycnXzd4lwdiCxZjDJSG8> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl2MEexNkc,OjV7NEC3NVI9N2F-
MV-4-11 NHv0[pRCeG:ydH;zbZMhSXO|YYm= NFrxbG8zNjVizszN NVXKb3dWPDhiaB?= NXfxUJFocW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> NVrkeIc6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNVE2QDNpPkK1NVEyPTh|PD;hQi=>
THP-1 M{G5NWFxd3C2b4Ppd{BCe3OjeR?= M4j0fFIvPSEQvF2= NX;3TVVoPDhiaB?= M{TpXYlv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 MkC5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMUG1PFMoRjJ3MUGxOVg{RC:jPh?=
MOLM 13 NIPFe4tCeG:ydH;zbZMhSXO|YYm= MnLwNk42KM7:TR?= MVq0PEBp NEDUbJFqdmS3Y3XzJIFxd3C2b4Ppd{B{dGmpaITsfS=> MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTFzMUW4N{c,OjVzMUG1PFM9N2F-
KBM3/Bu2506 MV\BdI9xfG:|aYOgRZN{[Xl? NH;TPW4zNjVizszN NHjoeJM1QCCq MWrpcoR2[2W|IHHwc5B1d3OrczDzcIlocHSueR?= NV3XVVlQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNVE2QDNpPkK1NVEyPTh|PD;hQi=>
Nalm-6 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\ZTWM2OD1zODFOwG0> MoewQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNkGxNFEoRjJ3ME[xNVAyRC:jPh?=
Reh NIDUenBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnHYTWM2OD1|MDFOwG0> NEPtNFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2NVExOSd-MkWwOlEyODF:L3G+
U2937 NY\3[JVIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M37CV2lEPTB;MU[g{txO NXLoSZJLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOlEyODFpPkK1NFYyOTBzPD;hQi=>
Mec-1 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV7JR|Ux97zgNUCwJO69VQ>? NYfyTnFyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOlEyODFpPkK1NFYyOTBzPD;hQi=>
RPMI-8226 M1f0b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzoXmlsUUN3ME21NFAh|ryP MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB4MUGwNUc,OjVyNkGxNFE9N2F-
Molt-4 MmnsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlO3TWM2OD1zOECg{txO MofaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNkGxNFEoRjJ3ME[xNVAyRC:jPh?=
Nalm-6-FluR NX\1TIV1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF;abZdKSzVyPUK1NEDPxE1? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB4MUGwNUc,OjVyNkGxNFE9N2F-
Raji  Ml3rSpVv[3Srb36gRZN{[Xl? NIfRXHQ{yqEQvF2= NVLv[HFGOjRxNEivO|IhcA>? M4rNUolv\HWlZYOgZYNkfW23bHH0bY9veyCxZjDwOVMtKHB4MzDhcoQheDd|wrC= NI\UZpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm0NFY6PSd-MkS5OFA3QTV:L3G+
PBMC NGGxfoZHfW6ldHnvckBCe3OjeR?= M{PmfFUxNzFyMDFOwG0> MYiyOEBp MULEUXNQ NXjM[m82cW6qaXLpeJMhW1SDVEGgdIhwe3Cqb4L5cIF1cW:w NH;DbXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmxNVg4Oid-MkS5NVE5PzJ:L3G+
MDA-231 M1XZNWZ2dmO2aX;uJGF{e2G7 NIf2V5oyODBizszN MkDuNlQhcA>? NY[4[lhSTE2VTx?= M1HM[oRm[3KnYYPld{BKTE9iZYjwdoV{e2mxbh?= NGLLT2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmxNVg4Oid-MkS5NVE5PzJ:L3G+
624.38mel  MV;GeY5kfGmxbjDBd5NigQ>? M2faTlUxKM7:TR?= M2PqclI1KGh? M2myNWROW09? NYTrOGNb\GWlcnXhd4V{KEmGTzDlfJBz\XO|aX;u NWLXUpB1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NVE5PzJpPkK0PVEyQDd{PD;hQi=>
MDA-231 MXLGeY5kfGmxbjDBd5NigQ>? M1XsZlUxNTJyMDFOwG0> NFH6OGYzPCCq NEjQ[VJFVVOR NFTZR5FqdmirYnn0d{BKTE9iYXP0bZZqfHliaX7k[ZBmdmSnboTsfUBw\iCvUl7BJIxmfmWucx?= MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlzMUi3Nkc,OjR7MUG4O|I9N2F-
624.38mel  MW\GeY5kfGmxbjDBd5NigQ>? NIjLOlE2OC1{MECg{txO NYrkdWhyOjRiaB?= NWDYXI9kTE2VTx?= MYPpcohq[mm2czDJSG8h[WO2aY\peJkhcW6mZYDlcoRmdnSueTDv[kBuWk6DIHzleoVtew>? MmHOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MUG4O|IoRjJ2OUGxPFczRC:jPh?=
HMECs NH3oO5VHfW6ldHnvckBCe3OjeR?= MVKxNFDDqM7:TdMg M4L6d|M3yqCq NWLocVdmcW6qaXLpeJMhUU[QzsRCpIFv\CCOUGOgbY5lfWOnZDDTWGFVOSCyaH;zdIhwenmuYYTpc44h[W6mIFnSSlEh\XiycnXzd4lwdg>? NYTZPZQ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNVE{OjdpPkK0NlEyOzJ5PD;hQi=>
HMECs  M4m1SGZ2dmO2aX;uJGF{e2G7 M{TUdVExOMLizszNxsA> M2XkcFM3yqCq NEniW25qdmirYnn0d{BKTk8QsdMgcYVlcWG2ZXSgdIhwe3Cqb4L5cIF1cW:wIH;mJHNVSVRzIHHu[EBUXEGWMzygZpV1KG6xdDDv[kBUXEGWMh?= M37UclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkGxN|I4Lz5{NEKxNVMzPzxxYU6=
BJAB NXLtNFFwSXCxcITvd4l{KEG|c3H5 NVrrbGNrPcLizszN NEPKfpczPCCq Moj3bY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB3N{G0O{c,OjRyNUexOFc9N2F-
I-83 NEfZZVhCeG:ydH;zbZMhSXO|YYm= NHOyWIc2yqEQvF2= MnjCNlQhcA>? MVLpcoR2[2W|IHPlcIwh[XCxcITvd4l{ MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB3N{G0O{c,OjRyNUexOFc9N2F-
NALM6 NVr3cXZUSXCxcITvd4l{KEG|c3H5 NFHKPWY2yqEQvF2= NYrSSFFROjRiaB?= NXLI[XhNcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= NG\RNFQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC1O|E1Pyd-MkSwOVcyPDd:L3G+
DU-145 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVywMVExKM7:Zz;tcC=> NVfGU|lXPDhiaNMg M1G2T4lvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd|NEixOUc,OjN5M{S4NVU9N2F-
Nalm-6 NELSWJpHfW6ldHnvckBCe3OjeR?= MmO3NVDDqM7:TR?= MoW4NU8zNzRiaB?= NV3iR|BNcW6mdXPld{BifXSxcHjh[5k> MortQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4OEGyNlMoRjJ|NkixNlI{RC:jPh?=
Reh M4LwTGZ2dmO2aX;uJGF{e2G7 MlnYNVDDqM7:TR?= NXiwepZvOS9{L{SgbC=> M3zXTYlv\HWlZYOgZZV1d3CqYXf5 M2TL[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkixNlI{Lz5{M{[4NVIzOzxxYU6=
Nalm-6 NXX4dFRWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE[zS2lKSzVyIPMIwFEx6oDLzszN NWfk[WNnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFEzOjNpPkKzOlgyOjJ|PD;hQi=>
Reh MnvNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3hPIExUUN3MDFijNwyOOLCid88US=> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ6MUKyN{c,OjN4OEGyNlM9N2F-
HEC1A Mlq5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVuxNFAuPTByIN88US=> MU[yOEBp NUHvO2xqcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NYfnWoRVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVU3QTdpPkKzOVk2Pjl5PD;hQi=>
AN3CA Mn\CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\wWmYxOTByLUWwNEDPxE1? NIXyV5AzPCCq MX;pcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NVzk[JVpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVU3QTdpPkKzOVk2Pjl5PD;hQi=>
HEC50B M3nzTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF3hPWIyODBvNUCwJO69VQ>? NHXoflczPCCq NH7yWW9qdmirYnn0d{Bk\WyuIHfyc5d1cCC|bHnnbJRtgQ>? NF[5VmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W5OVY6Pyd-MkO1PVU3QTd:L3G+
HEC1A NGixS3lCeG:ydH;zbZMhSXO|YYm= MlXCNlAwOTByIN88US=> NGKyc|YzPCCq MmnmbY5lfWOnczDhdI9xfG:|aYOgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NGDDeI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W5OVY6Pyd-MkO1PVU3QTd:L3G+
AN3CA MV\BdI9xfG:|aYOgRZN{[Xl? NXXlTVVXOjBxMUCwJO69VQ>? NILx[m4zPCCq NEC1U3lqdmS3Y3XzJIFxd3C2b4Ppd{BqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV7NU[5O{c,OjN3OUW2PVc9N2F-
HEC50B MUfBdI9xfG:|aYOgRZN{[Xl? MVqyNE8yODBizszN MnS2NlQhcA>? MlrFbY5lfWOnczDhdI9xfG:|aYOgd4xq\2i2bIm= Mn70QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3OUW2PVcoRjJ|NUm1Olk4RC:jPh?=
EHEB MUjBdI9xfG:|aYOgRZN{[Xl? MUO0NEDPxE1? MnfTNlQhcA>? MWXpcoR2[2W|IHHwc5B1d3Orcx?= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR7N{C3OUc,OjN2OUewO|U9N2F-
A549 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTF3LkhCtVIvQCEEtV2= Mnz4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|N{exPVIoRjJ|M{e3NVkzRC:jPh?=
A549 GAPDH-deficient NXL6O3RoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoDLTWM2OD1zOD61xtEzNjNiwsXN NFzYVos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O3O|E6Oid-MkOzO|cyQTJ:L3G+
CLL  M3\sdmFxd3C2b4Ppd{BCe3OjeR?= MYmxNEDPxE4EoB?= Ml;oNlQuQTZiaB?= M3nOd4lv\HWlZYOgZZBweHSxdHnjJINmdGxiZHXheIg> NX36Omo2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyNFc3QDZpPkKyNlA4Pjh4PD;hQi=>
MEC1 MVHBdI9xfG:|aYOgRZN{[Xl? MV:xNFDDqM7:TR?= NFjUcm04OiCq MorCbY5lfWOnczDhdI9xfG:|aYOgd4lodmmoaXPhcpRtgQ>? NU\jVGM1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxN|I6PzNpPkKyNVMzQTd|PD;hQi=>
U937  M{XHfmFxd3C2b4Ppd{BCe3OjeR?= NWrMOFZIOC56IN88US=> NHf1[pM1NTR6IHi= MlzpbY5lfWOnczDhdI9xfG:|aYOgd4xq\2i2bIm= M1ToSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEe0O|AxLz5{MkC3OFcxODxxYU6=
U937  MlHKRZBweHSxc3nzJGF{e2G7 MWqxJO69VQ>? Mmj1PVYhcA>? M3rXTolv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 M4HKSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEKzOVI{Lz5{MkCyN|UzOzxxYU6=
Daudi MXPBdI9xfG:|aYOgRZN{[Xl? MmrLNlAh|ryP MUO5OkBp M3jOeIlv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 NGnDOos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkCyN|UzOyd-MkKwNlM2OjN:L3G+
J45.01 MU\BdI9xfG:|aYOgRZN{[Xl? NXzGc21OOSEQvF2= NHrDSHk6PiCq MljDbY5lfWOnczDhdI9xfG:|aYOgd4xq\2i2bIm= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB{M{WyN{c,OjJyMkO1NlM9N2F-
RPMI 8226 Mlf6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYS1cYRRUUN3ME2yOU46yqEEsdMgN{44KM7:TR?= MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl2OEK2OEc,OjF7NEiyOlQ9N2F-
CEM MmfKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWnJR|UxRTJwNNMgxtHDqDBwNDFOwG0> NVzCU|g6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5OFgzPjRpPkKxPVQ5OjZ2PD;hQi=>
Raji MmjsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnXHTWM2OD1yLkS3xsDDucLiMD6wOEDPxE1? MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl2OEK2OEc,OjF7NEiyOlQ9N2F-
U937 M{\QfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWP2XWJVUUN3ME2wMlI1yqEEsdMgNE4xPCEQvF2= NHK1OoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUm0PFI3PCd-MkG5OFgzPjR:L3G+
K562 MlH6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTBwNEVCpOKyyqByLkC1JO69VQ>? NETqN289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUm0PFI3PCd-MkG5OFgzPjR:L3G+
NALM-6 M1\KbGFxd3C2b4Ppd{BCe3OjeR?= NE\FNGoyOCEQvF5CpC=> MVSyOEBp MVLpcoR2[2W|IHPlcIwh[XCxcITvd4l{KHOuaXfoeIx6 MlSwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF4OUmzPFMoRjJzNkm5N|g{RC:jPh?=
JMV-3 NHeydnBCeG:ydH;zbZMhSXO|YYm= NX7K[m9xOTBizszNxsA> MX6yOEBp NUn1NVRqcW6mdXPld{Bk\WyuIHHwc5B1d3OrczDzcIlocHSueR?= NVS2UnZIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG2PVk{QDNpPkKxOlk6Ozh|PD;hQi=>
EHEB M4e0bWZ2dmO2aX;uJGF{e2G7 NX32ToFDPS13MDFOwG0> NVXoUWhHOjRiaB?= NUDUS5gz\GWlcnXhd4V{KHB{MTDlfJBz\XO|aX;uJJNq\26rZnnjZY51dHl? NFrWdY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUG2PFM6OSd-MkGxOlg{QTF:L3G+
JVM-2  MYPGeY5kfGmxbjDBd5NigQ>? M{XhUFMxKM7:TR?= NWnsXmZsOjRiaB?= MXnk[YNz\WG|ZYOgdFIyKGW6cILld5Nqd25? NX3JZWU6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxOlg{QTFpPkKxNVY5OzlzPD;hQi=>
KBM3/Bu2506 NXHsSpNwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH;3dHdKSzJyPUCuOlchyrWP NH;mRYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEmzN|UxQSd-MkC5N|M2ODl:L3G+
KBM3/Bu2506 MoP6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWSwMlYh|ryP NEftW|AzPCCq MX7pcoNz\WG|ZYOgeIhmKGOnbHyg[pJi[3Srb36gbY4hWy2yaHHz[S=> MlqxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB7M{O1NFkoRjJyOUOzOVA6RC:jPh?=
MDA-MB-231 MljvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxRTRwMDFOwG0> M{O2N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNES3N|kxLz5{MES0O|M6ODxxYU6=
MCF-7 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHvtNpFKSzVyPUG1MlAh|ryP NVjSTZJURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0OFc{QTBpPkKwOFQ4OzlyPD;hQi=>
HLE-B3  NVfFTVlCTnWwY4Tpc44hSXO|YYm= NVvBWFlCOjVizszN M{HWWlQ5KGh? MVHicI9kc3NiSV\OMe6{6oDVaX7keYNm\CCVVFHUNUBxcG:|cHjvdplt[XSrb36gZY5lKEmGTzDlfJBz\XO|aX;u MnqyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2M{WxOVgoRjJyNEO1NVU5RC:jPh?=
K562 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlrMO|IhcA>? Mmq0TWM2OD1|LkOgcm0> M2fKcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyM{C3NVk5Lz5{MEOwO|E6QDxxYU6=
BW-225 M4j4VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUL4fHU1UUN{ME2xMlM4KMPZMUFijLI5yqEQvF5CpC=> MoTSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4NkGzPFAoRjF6Nk[xN|gxRC:jPh?=
OH-65 MnXtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVrPOJhIUUN{ME2xMlM4KMPZMUFijLI5yqEQvF5CpC=> MoG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4NkGzPFAoRjF6Nk[xN|gxRC:jPh?=
GR-145 NHnnRpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXfzUJhIUUN{ME2yMlc1KMPZIEGw5qiTQCBizszNxsA> NX;WTHJGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2OlE{QDBpPkG4OlYyOzhyPD;hQi=>
A549 NGjZ[5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1n4ZWlEOjB;NT60PEDEnyBzMPMIllgh|ryPwrC= NF7KVXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[2NVM5OCd-MUi2OlE{QDB:L3G+
CaSki  NEfnbFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGL0WZhKSzJyPUGuN|chy5diMUFijLI4KM7:TdMg NVrKO41ZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2OlE{QDBpPkG4OlYyOzhyPD;hQi=>
ZMK-1 NIHx[o1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|IxRTFwM{egx7chOTEkiKK2JO69VcLi NVzNZ5pWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2OlE{QDBpPkG4OlYyOzhyPD;hQi=>
SKW6.4 MYnBdI9xfG:|aYOgRZN{[Xl? M4fGNVAvODFvMUCg{txO M{LXbVI1NzR6IHi= NH;hbZlqdmS3Y3XzJINmdGxiZHXheIghcW5iYn;0bEB1cW2nLTDhcoQh\G:|ZT2g[IVx\W6mZX70JI1idm6nch?= NES5O4M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEC5NlM1OCd-MUiwPVI{PDB:L3G+
RPMI 8226 NFfvdFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkL2NlQhcA>? NFPTTWZKSzVyPUGuOVTDqM7:TR?= M1q0U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUe2NVg3Lz5zN{m3OlE5PjxxYU6=
MM.1S NIHzNIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVHCe5MxPDhiaB?= M3LGZ2lEPTB;MUOuOFjDqM7:TR?= M3PwUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUe2NVg3Lz5zN{m3OlE5PjxxYU6=
MM.1R NEfsUHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\LOFghcA>? NUXRfHpNUUN3ME2zN{44QSEQvF2= NI[2[Yw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{m3OlE5Pid-MUe5O|YyQDZ:L3G+
U937 MkjXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDQdIJKSzVyPUOsNlAxKMLzIEW2NEBvVQ>? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTl|MEO2NUc,OTV7M{CzOlE9N2F-
CLL5 M3vBW2FvfGm2dX3vdkBie3OjeR?= NFHLR3I1QCCqcoO= MYPBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDDUGw2KGOnbHzzJIl{d2yjdHXkJIZzd21iQ1zMJJBifGmnboSgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiNEigbJJ{KGK7IF\BR3Mh[W6jbInzbZMtKEWFNUCgQUAxNjF4IN88UU4> MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ5M{ezPUc,OjR4N{O3N|k9N2F-
K562 MnzwR5l1d3SxeHnjbZR6KGG|c3H5 M1;WUFczKGi{cx?= NVrNcIJpS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4heGGlbHn0ZZhmdC2{ZYPpd5RidnRiS{W2NkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMD6yOkDPxE1w MnfyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB4MEW2OVYoRjJyNkC1OlU3RC:jPh?=
CLL3 MnHkRY51cXS3bX;yJIF{e2G7 NXzmcpc6PDhiaILz NITOV45CdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBEVEx|IHPlcIx{KGm|b3zheIVlKG[{b32gR2xNKHCjdHnlcpQh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JGZCS1NiYX7hcJl{cXNuIFXDOVAhRSByLkO1JO69VS5? MkCzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4N{O3N|koRjJ2NkezO|M6RC:jPh?=
CLL4 NFzqd3ZCdnSrdIXtc5Ih[XO|YYm= NWLDWWx[PDhiaILz MlXNRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgR2xNPCClZXzsd{Bqe2:uYYTl[EBnem:vIFPMUEBx[XSrZX70JIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDGRWNUKGGwYXz5d4l{NCCHQ{WwJF0hOC54NDFOwG0v MoXnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4N{O3N|koRjJ2NkezO|M6RC:jPh?=
CEM-DNR-B Mmn1R5l1d3SxeHnjbZR6KGG|c3H5 MkHJO|IhcHK| NUP1UFlHS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hS0WPLVTOVk1DKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCxMlAyKM7:TT6= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODZyNU[1Okc,OjB4MEW2OVY9N2F-
primary CLL Mnn5R5l1d3SxeHnjbZR6KGG|c3H5 MWXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDwdolu[XK7IFPMUEBk\WyuczygUGQ2OCB;IEGuNUDPxE1w NXXDenFHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxOFg{QTJpPkK1NVQ5Ozl{PD;hQi=>
CLL6 M2SxeWFvfGm2dX3vdkBie3OjeR?= NWfPcmhNPDhiaILz M3iwOGFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JGNNVDZiY3XscJMhcXOxbHH0[YQh\nKxbTDDUGwheGG2aXXueEBie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjC0PEBpenNiYomgSmFEWyCjbnHsfZNqeyxiRVO1NEA:KDFwNjFOwG0v M3nYWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkezO|M6Lz5{NE[3N|c{QTxxYU6=
CLL2 MWPBcpRqfHWvb4KgZZN{[Xl? MnTUOFghcHK| MmX0RY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgR2xNOiClZXzsd{Bqe2:uYYTl[EBnem:vIFPMUEBx[XSrZX70JIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDGRWNUKGGwYXz5d4l{NCCHQ{WwJF0hOi54NjFOwG0v NUTqe5lWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2O|M4OzlpPkK0Olc{PzN7PD;hQi=>
HCT116 Mn;qR5l1d3SxeHnjbZR6KGG|c3H5 M1TRUFczKGi{cx?= MlfmR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVOTF4IHPlcIx{KGGodHXyJFczKGi{czDifUBUWkJiYYPzZZktKEmFNUCgQUA3NjZizszNMi=> M4nCclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NE[yNlc4Lz5{NUS2NlI4PzxxYU6=
PBMC NV\Cc2N6S3m2b4TvfIlkcXS7IHHzd4F6 M4C3OlQ5KGi{cx?= M1r3[GN6fG:2b4jpZ4l1gSCjZ3HpcpN1KHCjdHnlcpQhWEKPQzDh[pRmeiB2ODDodpMh[nliQ3XscHRqfHKnLVLseYUh[XO|YYmgbY4heHKnc3XuZ{Bw\iCvb4Xz[UBOOjFyQkSgZ4VtdHNuIFnDOVAhRSBzMDFOwG0v M1naUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NU[yOFE4Lz5{NUW2NlQyPzxxYU6=
CHO NF\SN|VHfW6ldHnvckBie3OjeR?= NXrNb3h3SmmwZHnu[{Bi\m[rbnn0fUBl\XSncn3pcoVlKGK7IHTpd5Bt[WOnbXXueEBw\iC|cHXjbYZq[yCkaX7kbY5oKG:oIGuxNlVKZU5vKEStZY1qdm9vMz3pc4RweGinbnX0bJltMS2jZHXuc5NqdmViaX6gcYVu[nKjbnXzJI9nKEOKTzDj[YxteyC|dHHicJkhfHKjboPm[YN1\WRid3n0bEB1cGVicnH0JIFl\W6xc3nu[UBCOyC{ZXPldJRweixiS3mgQUAyOC52IN88UU4> M4XFWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzd5MEezNlAoRjd5MEezNlA9N2F-
JVM2 M2PDbmFvfGm2dX3vdkBie3OjeR?= MX7BcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCMVl2yJINmdGy|IHHzd4V{e2WmIHHzJINmdGxidnnhZoltcXS7IHHmeIVzKDR6IHjyd{BjgSCIQVPTJIFv[Wy7c3nzMEBGSzVyIE2gNVAvPCEQvF2u Moq1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4N{O3N|koRjJ2NkezO|M6RC:jPh?=
HeLa M1n1NWFvfGm2dX3vdkBie3OjeR?= NFnWNW5CdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIJ6KE2WVDDhd5NigSxiRVO1NEA:KDF4IN88UU4> MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ5M{ezPUc,OjR4N{O3N|k9N2F-
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BT-12 MlvsdWhVWyCjc4PhfS=> NXj6b244eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFLUMVEzKGOnbHzz MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NB1643 MnvPdWhVWyCjc4PhfS=> MoPudWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6EMU[0N{Bk\Wyucx?= NIHweYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
OHS-50 MnHZdWhVWyCjc4PhfS=> MYXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| M1X5bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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SK-N-SH MXzxTHRUKGG|c3H5 NV7uTWtEeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW0tvTj3TTEBk\Wyucx?= NGHJUJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
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BT-37 Ml;RdWhVWyCjc4PhfS=> M3XXRZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGJVNTN5IHPlcIx{ NWjxfoN4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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SK-N-SH MlnUdWhVWyCjc4PhfS=> M4\XdpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KE:{dHjv[49v[WxiM1Sgeoli[mmuaYT5JJNkemWnbjDmc5IhW0tvTj3TTEBk\Wyucx?= NV\VNYpSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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Daoy NWHYNYNOeUiWUzDhd5NigQ>? MXPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBQenSqb3fvcoFtKDOGII\pZYJqdGm2eTDzZ5Jm\W5iZn;yJGRid3liY3XscJM> Mom5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
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LAN-5 NXrD[YdyeUiWUzDhd5NigQ>? NV6zV5E2eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFzBUk02KGOnbHzz MoPDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh18 MY\xTHRUKGG|c3H5 NG\1b4VyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUnixPEBk\Wyucx?= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NB1643 MYfxTHRUKGG|c3H5 MkfmdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUmIyPjR|IHPlcIx{ M4nhSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-MC MV3xTHRUKGG|c3H5 M{D1RZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNMNU5vTVOgZ4VtdHN? MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SJ-GBM2 MlrhdWhVWyCjc4PhfS=> MWLxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTTk1ISk1{IHPlcIx{ MmS3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
TC32 MlTIdWhVWyCjc4PhfS=> MUHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDUR|MzKGOnbHzz Mn3CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh18 M3TUN5FJXFNiYYPzZZk> M2XCW5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHJpOThiY3XscJM> MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Saos-2 MUDxTHRUKGG|c3H5 MYDxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTZY9{NTJiY3XscJM> Mk[wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SJ-GBM2 M{fhS5FJXFNiYYPzZZk> NXrue4Q2eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiT4L0bI9od26jbDCzSEB3cWGkaXzpeJkhe2O{ZXXuJIZweiCVSj3HRm0zKGOnbHzz MoPxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
RD NI\NR3JyUFSVIHHzd4F6 NHr1O4pyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCRcoToc4dwdmGuIEPEJJZq[WKrbHn0fUB{[3KnZX6g[o9zKFKGIHPlcIx{ MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-

他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

アッセイ
Methods Test Index PMID
Western blot
procaspase-9 / procaspase-3; 

PubMed: 27223263     


Procaspase-9 and procaspase-3 were analyzed by Western blot in CLL cells from three patients after a 48 h-incubation in the absence or presence of 3 μM aphidicolin (APC) with or without fludarabine at 0.3 and 1 μM. β-Actin served as a loading control.

p-p53 / p53; 

PubMed: 27223263     


(A–B) CLL cells from 3 different patients were incubated for 24 h with fludarabine at the indicated concentrations in the absence or presence of 3 μM aphidicolin (APC). Phosphorylation of p53 at Ser-15 and total p53 were analyzed by Western blot. Data sho䲧疝Ỵ疞㧀疜膉痘 瘿�෋ᾰƌ෋à 㺣痖帉痖Ѐ瑖堘𢡄빢᎒෋à鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒

STAT1; 

PubMed: 26677135     


Representative western blot analysis of STAT1 in RAW 264.7 cells treated with STAT1 inhibitor fludarabine (Flu) for 24 h.

27223263 26677135
Immunofluorescence
α-SMA / Vimentin; 

PubMed: 28322315     


FLU (50 μM) reduces the expression of α-SMA and Vimentin protein in primary mouse activated HSCs (Hepatic Stellate Cells).

28322315
Growth inhibition assay
Cell viability ; 

PubMed: 24956101     


CLL cells RPMI/0.1% FBS were cultured on 0.5% BSA or 150 nM MMP-9 for 1 h prior to adding the indicated concentrations of fludarabine (Fluda). After 48 h (Fluda) cell viability was determined by flow cytometry using FITC-Annexin V and PI.

24956101
体内試験 Tumors treated with PBS grow rapidly to approx-imately 10-fold their initial volume in 25 day, whereas, the tumors in the Fludarabine at 40 mg/kg increase less than 5-fold. A significant antitumor effect of 40 mg/kg Fludarabine on RPMI8226 tumor growth is demonstrated. RPMI8226 tumors treated with 40 mg/kg Fludarabine at day 10 increase apoptotic nuclei. Fludarabine is effective in suppressing RPMI8226 myeloma xenografts in SCID mice. [1]

お薦めの試験操作(参考用のみ)

細胞試験:

[1]

- 合併
  • 細胞株: Dexamethasone-sensitive (MM.1S) and -resistant (MM.1R) human MM cell lines, RPMI8226 and U266 cell lines
  • 濃度: 2 μg/mL
  • 反応時間: 24 hours
  • 実験の流れ:

    After treated with Fludarabine or control, dexamethasone-sensitive (MM.1S) and -resistant (MM.1R) human MM cell lines, RPMI8226 and U266 cell lines (5 × 105 cells) are washed twice in phosphate-buffered saline (PBS) and fixed with 70% ice-cold ethanol, then centrifuged and suspended in PBS containing 100 μg/mL RNase A. After incubated for 30 minutes at 37 ºC, samples are resuspended in 25 μg/mL propidium iodide. Flow cytometry is performed on a FACSCalibur automated system. Apoptosis is determined by Annexin V-FITC apoptosis detection kit, according to the manufacturer's instructions. For TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling) assay, cells are analyzed by flow cytometry using the in situ cell death detection kit.


    (参考用のみ)
動物試験:

[1]

- 合併
  • 動物モデル: Severe combined immunodeficient (SCID) mice bearing RPMI 8226 cells
  • 投薬量: 40 mg/kg
  • 投与方法: Administered via i.p.
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 57 mg/mL (199.83 mM)
Water Insoluble
Ethanol Insoluble
体内 左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O
混合させたのち直ちに使用することを推奨します。
8 mg/mL

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。

化学情報

分子量 285.23
化学式

C10H12FN5O4

CAS No. 21679-14-1
Storage powder
in solvent
別名 FaraA, Fludarabinum
Smiles C1=NC2=C(N=C(N=C2N1C3C(C(C(O3)CO)O)O)F)N

投与溶媒組成計算器(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)
投与量 mg/kg 動物平均体重 g 投与体積(動物毎) ul 動物数
ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)
% DMSO % % Tween 80 % ddH2O
計算リセット

便利ツール

モル濃度計算器

モル濃度計算器

求めたい質量、体積または濃度を計算してください。

質量 (mg) = 濃度 (mM) x 体積 (mL) x 分子量 (g/mol)

モル濃度計算器方程式

  • 質量
    濃度
    体積
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備するために必要な希釈率を計算してください。Selleck希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 入力 出力 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

質量 濃度 体積 分子量

臨床試験

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04526795 Not yet recruiting Drug: Cytarabine|Drug: Fludarabine|Drug: Pegcrisantaspase Blast Phase Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent Acute Biphenotypic Leukemia|Recurrent Acute Lymphoblastic Leukemia|Recurrent Acute Myeloid Leukemia|Recurrent Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent T-Cell Prolymphocytic Leukemia|Refractory Acute Biphenotypic Leukemia|Refractory Acute Lymphoblastic Leukemia|Refractory Acute Myeloid Leukemia|Refractory Chronic Myelogenous Leukemia BCR-ABL1 Positive|Refractory T-Cell Prolymphocytic Leukemia M.D. Anderson Cancer Center|National Cancer Institute (NCI) December 31 2020 Phase 1
NCT03832127 Not yet recruiting Drug: 18F-Fludarabine Myeloma Nantes University Hospital|Cyceron April 1 2019 Phase 1
NCT03696537 Active not recruiting Drug: Fludarabine|Radiation: Total Marrow Irradiation (TMI) Acute Myeloid Leukemia|Chronic Myeloid Leukemia|Acute Lymphocytic Leukemia|Myelodysplastic Syndromes Naoyuki G. Saito M.D. Ph.D.|Indiana University August 29 2018 Phase 1
NCT02718755 Withdrawn Drug: Fludarabine|Drug: Cytarabine|Drug: Erwinase Hematologic Malignancy M.D. Anderson Cancer Center|Jazz Pharmaceuticals May 2018 Phase 2

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

  • * 必須

よくある質問(FAQ)

  • 質問1:

    how to re-suspend and deliver the inhibitor for in vivo experiments?

  • 回答:

    For S1491, Fludarabine, we tested a vehicle: 30% Propylene glycol, 5% Tween 80, 65% D5W that you can resuspend the compound in at up to 30mg/ml. It's a suspension and can only be given via oral gavage.

STATシグナル伝達経路

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