Fludarabine (NSC 118218)

For research use only. Not for use in humans.

製品コードS1491 別名:FaraA, Fludarabinum

Fludarabine (NSC 118218)化学構造

CAS No. 21679-14-1

Fludarabine (NSC 118218, FaraA, Fludarabinum) is a STAT1 activation inhibitor which causes a specific depletion of STAT1 protein (and mRNA) but not of other STATs. Also a DNA synthesis inhibitor in vascular smooth muscle cells. Fludarabine induces apoptosis.

サイズ 価格(税別)  
10mM (1mL in DMSO) JPY 22900
JPY 18100
JPY 53400
JPY 130160
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バルク問合せ

文献中Selleckの製品使用例(114)

製品安全説明書

STAT阻害剤の選択性比較

生物活性

製品説明 Fludarabine (NSC 118218, FaraA, Fludarabinum) is a STAT1 activation inhibitor which causes a specific depletion of STAT1 protein (and mRNA) but not of other STATs. Also a DNA synthesis inhibitor in vascular smooth muscle cells. Fludarabine induces apoptosis.
ターゲット
STAT1 [4]
(Vascular smooth muscle cells)
体外試験

Fludarabine efficiently inhibits the proliferation of RPMI 8226 cells with IC50 of 1.54 μg/mL. The IC50 of Fludarabine against MM.1S and MM.1R cells is 13.48 μg/mL and 33.79 μg/mL, respectively. In contrast, U266 cells are resistant to Fludarabine with IC50 of 222.2 μg/mL. Fludarabine treatment results in increased number of cells in the G1 phase of cell cycle, accompanied with a concomitant reduction of cells at the S phase of cell cycle in a time-dependent manner. Fludarabine induces a cell cycle block and triggers apoptosis in MM cells. Fludarabine triggers time-dependent cleavage of caspase-8, -9, and -3, -7, followed by PARP cleavage. Fludarabine increases expression of Bax in a time-dependent fashion, while the expression of Bak doesn't change. After exposure to Fludarabine for 12 hours, RPMI 8226 cells shows a loss of membrane potential with 61.05% of the cells expressing low fluorescence of rhodamine 123 compared with 8.62% of cells in untreated control. [1] To enhance solubility, Fludarabine is formulated as the monophosphate (F-ara-AMP, fudarabine), which is instantaneously and quantitatively dephosphorylated to the parent nucleoside upon intravenous infusion. Inside the cells rephosphorylation occurs which leads to fuoroadenine arabinoside triphosphate (F-ara-ATP), the major cytotoxic metabolite of F-ara-A. [2] Fludarabine can also induce pro-inflammatory stimulation of monocytic cells, as evaluated by increased expression of ICAM-1 and IL-8 release. [3] Fludarabine does not affect the growth of ovarian cancer cell lines, whereas it induces marked and dose-dependent inhibition of proliferation in melanoma cell lines. [4] Fludarabine induces significant reduction of STAT-1 phosphorylation, whereas it does not change JAK2 activation. Interestingly, Fludarabine does not significantly affect the phosphorylation of these three STAT proteins. Fludarabine (1.5 mg) significantly prevents STAT-1 phosphorylation and also reduces the increased amount of this protein. No significant changes are demonstrated in JAK2 phosphorylation at 2 days, but Fludarabine inhibits JAK2-increased expression at 7 days. Fludarabine specifically inhibits STAT-1 activation without affecting other STAT proteins and consequently diminishes VSMC proliferation. [5]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Jeko-1  M376dmZ2dmO2aX;uJGF{e2G7 MkXENlAh|ryP NVXjZWZYOjRiaB?= NX;hdpRlcW6qaXLpeJMh\XiycnXzd4lwdiCxZjDJSG8> M{K1PVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUSwO|EzLz5{NUm0NFcyOjxxYU6=
MV-4-11 MlHjRZBweHSxc3nzJGF{e2G7 MUKyMlUh|ryP NWTwXIt7PDhiaB?= NEHuZ5dqdmS3Y3XzJIFxd3C2b4Ppd{B{dGmpaITsfS=> NIf6OlU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGxNVU5Oyd-MkWxNVE2QDN:L3G+
THP-1 MmLoRZBweHSxc3nzJGF{e2G7 Mm\HNk42KM7:TR?= M17uTFQ5KGh? NUPCO4lZcW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> MkHZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMUG1PFMoRjJ3MUGxOVg{RC:jPh?=
MOLM 13 M3HTemFxd3C2b4Ppd{BCe3OjeR?= NGXLN|YzNjVizszN M4my[|Q5KGh? M2rv[Ylv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTFzMUW4N{c,OjVzMUG1PFM9N2F-
KBM3/Bu2506 NVSxfpZUSXCxcITvd4l{KEG|c3H5 MXSyMlUh|ryP MXe0PEBp NVvHT|lzcW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTFzMUW4N{c,OjVzMUG1PFM9N2F-
Nalm-6 NFnTenhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlrTTWM2OD1zODFOwG0> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB4MUGwNUc,OjVyNkGxNFE9N2F-
Reh MmjES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV\JR|UxRTNyIN88US=> Mn22QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNkGxNFEoRjJ3ME[xNVAyRC:jPh?=
U2937 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NG\yXlRKSzVyPUG2JO69VQ>? NHT1S4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2NVExOSd-MkWwOlEyODF:L3G+
Mec-1 MlnuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|Ux97zgNUCwJO69VQ>? M4OyWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3ME[xNVAyLz5{NUC2NVExOTxxYU6=
RPMI-8226 M2\uNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoXNTWM2OD13MECg{txO MmjzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNkGxNFEoRjJ3ME[xNVAyRC:jPh?=
Molt-4 NGnsVVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnywTWM2OD1zOECg{txO MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB4MUGwNUc,OjVyNkGxNFE9N2F-
Nalm-6-FluR MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\MXGlEPTB;MkWwJO69VQ>? NWXmUmhpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOlEyODFpPkK1NFYyOTBzPD;hQi=>
Raji  NEjHbnRHfW6ldHnvckBCe3OjeR?= Ml3LN:Kh|ryP M4ezeFI1NzR6L{eyJIg> MYDpcoR2[2W|IHHjZ5VufWyjdHnvcpMhd2ZicEWzMEBxPjNiYX7kJJA4O8Li NULrXXpDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OFA3QTVpPkK0PVQxPjl3PD;hQi=>
PBMC MUTGeY5kfGmxbjDBd5NigQ>? NX;rUIExPTBxMUCwJO69VQ>? MU[yOEBp MUnEUXNQ MlrNbY5pcWKrdIOgV3RCXDFicHjvd5Bpd3K7bHH0bY9v NXrod2ZqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NVE5PzJpPkK0PVEyQDd{PD;hQi=>
MDA-231 MXfGeY5kfGmxbjDBd5NigQ>? Mn3zNVAxKM7:TR?= MX[yOEBp NX\qTIU3TE2VTx?= MnPR[IVkemWjc3XzJGlFVyCneIDy[ZN{cW:w MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlzMUi3Nkc,OjR7MUG4O|I9N2F-
624.38mel  NWPMUlcyTnWwY4Tpc44hSXO|YYm= NF7vRng2OCEQvF2= MVSyOEBp NGHy[3ZFVVOR M3rrUYRm[3KnYYPld{BKTE9iZYjwdoV{e2mxbh?= Ml3EQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MUG4O|IoRjJ2OUGxPFczRC:jPh?=
MDA-231 MnewSpVv[3Srb36gRZN{[Xl? MYO1NE0zODBizszN NHzhV28zPCCq NFru[I1FVVOR NWDDeJIycW6qaXLpeJMhUUSRIHHjeIl3cXS7IHnu[IVx\W6mZX70cJkhd2ZibWLORUBt\X[nbIO= Ml3kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MUG4O|IoRjJ2OUGxPFczRC:jPh?=
624.38mel  NIjD[JBHfW6ldHnvckBCe3OjeR?= MnjBOVAuOjByIN88US=> MXqyOEBp NHfveY9FVVOR NYLpXXFwcW6qaXLpeJMhUUSRIHHjeIl3cXS7IHnu[IVx\W6mZX70cJkhd2ZibWLORUBt\X[nbIO= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlzMUi3Nkc,OjR7MUG4O|I9N2F-
HMECs MYXGeY5kfGmxbjDBd5NigQ>? NYmyboltOTBywrFOwG3DqA>? NIXhU2c{PsLiaB?= NV7ucoxEcW6qaXLpeJMhUU[QzsRCpIFv\CCOUGOgbY5lfWOnZDDTWGFVOSCyaH;zdIhwenmuYYTpc44h[W6mIFnSSlEh\XiycnXzd4lwdg>? NHXudYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKxNVMzPyd-MkSyNVE{Ojd:L3G+
HMECs  NISzWFVHfW6ldHnvckBCe3OjeR?= NXXEcJZCOTBywrFOwG3DqA>? NF;ieIQ{PsLiaB?= M3XWR4lvcGmkaYTzJGlHVs7zwrDt[YRq[XSnZDDwbI9{eGixconsZZRqd25ib3[gV3RCXDFiYX7kJHNVSVR|LDDieZQhdm:2IH;mJHNVSVR{ M4\LVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkGxN|I4Lz5{NEKxNVMzPzxxYU6=
BJAB MV;BdI9xfG:|aYOgRZN{[Xl? NH;KXHA2yqEQvF2= MmjENlQhcA>? M2i1Xolv\HWlZYOgZ4VtdCCjcH;weI9{cXN? MlnFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyNUexOFcoRjJ2MEW3NVQ4RC:jPh?=
I-83 Mnm5RZBweHSxc3nzJGF{e2G7 MYe1xsDPxE1? MYmyOEBp NF7tNHNqdmS3Y3XzJINmdGxiYYDvdJRwe2m| M1jQT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEW3NVQ4Lz5{NEC1O|E1PzxxYU6=
NALM6 MmK5RZBweHSxc3nzJGF{e2G7 Mnq0OeKh|ryP MmTENlQhcA>? M4\YTIlv\HWlZYOgZ4VtdCCjcH;weI9{cXN? MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB3N{G0O{c,OjRyNUexOFc9N2F-
DU-145 NYDjTphuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LpVFAuOTBizsznM41t M2nFelQ5KGkEoB?= MlzrbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> M17IOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{O0PFE2Lz5{M{ezOFgyPTxxYU6=
Nalm-6 MY\GeY5kfGmxbjDBd5NigQ>? NVPTTHNHOTEEoN88US=> NIjFe3gyNzJxNDDo NYDuTYU5cW6mdXPld{BifXSxcHjh[5k> NWK5N|ZJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFEzOjNpPkKzOlgyOjJ|PD;hQi=>
Reh MoDqSpVv[3Srb36gRZN{[Xl? Mon6NVDDqM7:TR?= MXmxM|IwPCCq NWj3[YRucW6mdXPld{BifXSxcHjh[5k> NVXhepNGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFEzOjNpPkKzOlgyOjJ|PD;hQi=>
Nalm-6 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3PRdmlEPTBi4pk8NVDjiIoQvF2= M2nve|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkixNlI{Lz5{M{[4NVIzOzxxYU6=
Reh M1TZfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnuTWM2OCEkiMyxNQKBkc7:TR?= NH;LTpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[4NVIzOyd-MkO2PFEzOjN:L3G+
HEC1A NYPmZVVuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnu2NVAxNTVyMDFOwG0> NHvm[G4zPCCq NGf6NXZqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MmrMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3OUW2PVcoRjJ|NUm1Olk4RC:jPh?=
AN3CA M1nySGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIDuRlUyODBvNUCwJO69VQ>? NGrJWnozPCCq M3PZR4lvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NYfDN5lNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVU3QTdpPkKzOVk2Pjl5PD;hQi=>
HEC50B NWCyPVgxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M33LWVExOC13MECg{txO MoL5NlQhcA>? M{nhNYlvcGmkaYTzJINmdGxiZ4Lve5RpKHOuaXfoeIx6 NF[4XIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W5OVY6Pyd-MkO1PVU3QTd:L3G+
HEC1A NHfKS|dCeG:ydH;zbZMhSXO|YYm= NVHSTVRjOjBxMUCwJO69VQ>? NIPVbnEzPCCq MV;pcoR2[2W|IHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NGPMVXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W5OVY6Pyd-MkO1PVU3QTd:L3G+
AN3CA M2DxTWFxd3C2b4Ppd{BCe3OjeR?= Moe0NlAwOTByIN88US=> NXjV[VlROjRiaB?= MV7pcoR2[2W|IHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NFG1TVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W5OVY6Pyd-MkO1PVU3QTd:L3G+
HEC50B M2O3WmFxd3C2b4Ppd{BCe3OjeR?= MXGyNE8yODBizszN NGfvOI8zPCCq M3i4e4lv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 M1GyeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUm1Olk4Lz5{M{W5OVY6PzxxYU6=
EHEB NHnGc5BCeG:ydH;zbZMhSXO|YYm= NGHtSW41OCEQvF2= M{XPO|I1KGh? MVPpcoR2[2W|IHHwc5B1d3Orcx?= Mn[3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUewO|UoRjJ|NEm3NFc2RC:jPh?=
A549 NWDr[mx[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYi0bYdUUUN3ME2xOU44yrF{LkigxtVO NVHLdlU6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzO|cyQTJpPkKzN|c4OTl{PD;hQi=>
A549 GAPDH-deficient Ml3OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{O1SWlEPTB;MUiuOeKyOi5|INM1US=> MoDCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|N{exPVIoRjJ|M{e3NVkzRC:jPh?=
CLL  NYHNcoRqSXCxcITvd4l{KEG|c3H5 NX3iPY5lOTBizszNxsA> NHjoR3gzPC17NjDo NX3DfppicW6mdXPld{BieG:ydH;0bYMh[2WubDDk[YF1cA>? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJyN{[4Okc,OjJ{MEe2PFY9N2F-
MEC1 M3jJUGFxd3C2b4Ppd{BCe3OjeR?= Mn7sNVAxyqEQvF2= NHj3NoY4OiCq Mof2bY5lfWOnczDhdI9xfG:|aYOgd4lodmmoaXPhcpRtgQ>? NG\BcI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkGzNlk4Oyd-MkKxN|I6PzN:L3G+
U937  MoPkRZBweHSxc3nzJGF{e2G7 M{G2[VAvQCEQvF2= MXm0MVQ5KGh? M{\lOIlv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 Ml\wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyN{S3NFAoRjJ{MEe0O|AxRC:jPh?=
U937  NGDyVVVCeG:ydH;zbZMhSXO|YYm= NV7UfZh[OSEQvF2= NHnxdJI6PiCq MUDpcoR2[2W|IHHwc5B1d3OrczDzcIlocHSueR?= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB{M{WyN{c,OjJyMkO1NlM9N2F-
Daudi NV;GR3lISXCxcITvd4l{KEG|c3H5 MYCyNEDPxE1? MmrKPVYhcA>? MmPobY5lfWOnczDhdI9xfG:|aYOgd4xq\2i2bIm= MlfmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyMkO1NlMoRjJ{MEKzOVI{RC:jPh?=
J45.01 MnrpRZBweHSxc3nzJGF{e2G7 Ml;0NUDPxE1? NGntRoQ6PiCq M1raT4lv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 MmLsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyMkO1NlMoRjJ{MEKzOVI{RC:jPh?=
RPMI 8226 NVHMTVVFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml3hTWM2OD1{NT65xsDDucLiMz63JO69VQ>? M{HsflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUS4NlY1Lz5{MUm0PFI3PDxxYU6=
CEM NV3iWFZHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUnJR|UxRTJwNNMgxtHDqDBwNDFOwG0> MoTiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NEiyOlQoRjJzOUS4NlY1RC:jPh?=
Raji M1LFSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mmi0TWM2OD1yLkS3xsDDucLiMD6wOEDPxE1? NFTuW3o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUm0PFI3PCd-MkG5OFgzPjR:L3G+
U937 NW\Re2FOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXaWlVGUUN3ME2wMlI1yqEEsdMgNE4xPCEQvF2= MnfTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NEiyOlQoRjJzOUS4NlY1RC:jPh?=
K562 NFPufYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIfSV3VKSzVyPUCuOFTDqMLzwrCwMlA2KM7:TR?= NFnQU4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUm0PFI3PCd-MkG5OFgzPjR:L3G+
NALM-6 NGW4Z4tCeG:ydH;zbZMhSXO|YYm= MkXZNVAh|ryPwrC= NVP5cnN5OjRiaB?= NX;n[oF2cW6mdXPld{Bk\WyuIHHwc5B1d3OrczDzcIlocHSueR?= MmH5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF4OUmzPFMoRjJzNkm5N|g{RC:jPh?=
JMV-3 NIqyOlVCeG:ydH;zbZMhSXO|YYm= NF\5ZpYyOCEQvF5CpC=> MYCyOEBp M3PxZ4lv\HWlZYOgZ4VtdCCjcH;weI9{cXNic3zp[4h1dHl? MmrGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF4OUmzPFMoRjJzNkm5N|g{RC:jPh?=
EHEB M4\6UmZ2dmO2aX;uJGF{e2G7 M1z0WFUuPTBizszN NVHvenh3OjRiaB?= NInoT5Nl\WO{ZXHz[ZMheDJzIHX4dJJme3Orb36gd4lodmmoaXPhcpRtgQ>? NVzac5ZjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxOlg{QTFpPkKxNVY5OzlzPD;hQi=>
JVM-2  MnnMSpVv[3Srb36gRZN{[Xl? MlntN|Ah|ryP M2Dsb|I1KGh? MUfk[YNz\WG|ZYOgdFIyKGW6cILld5Nqd25? MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTF4OEO5NUc,OjFzNkizPVE9N2F-
KBM3/Bu2506 NGrOcoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVPJR|IxRTBwNkegxtVO M4D3NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOUOzOVA6Lz5{MEmzN|UxQTxxYU6=
KBM3/Bu2506 NGHSVHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXm5VmRkOC54IN88US=> NXTibmU2OjRiaB?= NIjnR4lqdmO{ZXHz[ZMhfGinIHPlcIwh\nKjY4Tpc44hcW5iUz3wbIF{\Q>? M4e3c|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOUOzOVA6Lz5{MEmzN|UxQTxxYU6=
MDA-MB-231 NXLmV2tLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mlu2TWM2OD12LkCg{txO NIXDfpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES0O|M6OCd-MkC0OFc{QTB:L3G+
MCF-7 NGD5cmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV\nSnNYUUN3ME2xOU4xKM7:TR?= M1LKWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNES3N|kxLz5{MES0O|M6ODxxYU6=
HLE-B3  M13WfWZ2dmO2aX;uJGF{e2G7 NFjl[oEzPSEQvF2= MXS0PEBp NIjVPGxjdG:la4OgTWZPNc7|4pETbY5lfWOnZDDTWGFVOSCyaH;zdIhwenmuYYTpc44h[W6mIFnEU{BmgHC{ZYPzbY9v NInwZ2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MESzOVE2QCd-MkC0N|UyPTh:L3G+
K562 NXL4XJFMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2npWlczKGh? NFnLdZlKSzVyPUOuN{BvVQ>? MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODNyN{G5PEc,OjB|MEexPVg9N2F-
BW-225 NUXLc3Q3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4T1Z2lEOjB;MT6zO{DEnzFy4pkSPOKh|ryPwrC= MkWwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4NkGzPFAoRjF6Nk[xN|gxRC:jPh?=
OH-65 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|IxRTFwM{egx7cyOOLKkklCpO69VcLi NVH6e4JzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2OlE{QDBpPkG4OlYyOzhyPD;hQi=>
GR-145 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXzufYZnUUN{ME2yMlc1KMPZIEGw5qiTQCBizszNxsA> NUjwOY5zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2OlE{QDBpPkG4OlYyOzhyPD;hQi=>
A549 M2PqSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEC0U4lKSzJyPUWuOFghy5diMUFijLI5KM7:TdMg M3W1RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6Nk[xN|gxLz5zOE[2NVM5ODxxYU6=
CaSki  MnPxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXrJR|IxRTFwM{egx7chOTEkiKK3JO69VcLi MnnwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4NkGzPFAoRjF6Nk[xN|gxRC:jPh?=
ZMK-1 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{HFXGlEOjB;MT6zO{DEnyBzMPMIllYh|ryPwrC= M4K2PFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6Nk[xN|gxLz5zOE[2NVM5ODxxYU6=
SKW6.4 NXTE[lVDSXCxcITvd4l{KEG|c3H5 NHvKSZUxNjBzLUGwJO69VQ>? NH\SOo4zPC92ODDo MlPXbY5lfWOnczDj[YxtKGSnYYToJIlvKGKxdHigeIlu\S1iYX7kJIRwe2VvIHTldIVv\GWwdDDtZY5v\XJ? NGPHem89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEC5NlM1OCd-MUiwPVI{PDB:L3G+
RPMI 8226 NUDBOI0xT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYrRWGpjOjRiaB?= MkfLTWM2OD1zLkW0xsDPxE1? NVT2VmZkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5O|YyQDZpPkG3PVc3OTh4PD;hQi=>
MM.1S NVm0TYJzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkDaOFghcA>? NIm5OIVKSzVyPUGzMlQ5yqEQvF2= NV;qOYdlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5O|YyQDZpPkG3PVc3OTh4PD;hQi=>
MM.1R MoTES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYPEOmc2PDhiaB?= Ml7JTWM2OD1|Mz63PUDPxE1? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzl5NkG4Okc,OTd7N{[xPFY9N2F-
U937 NYXrfGt3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3;YRWlEPTB;MzyyNFAhyrFiNU[wJI5O MkXTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV7M{CzOlEoRjF3OUOwN|YyRC:jPh?=
CLL5 MnnRRY51cXS3bX;yJIF{e2G7 M1PY[lQ5KGi{cx?= NUnLXIJ{SW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhS0yONTDj[YxteyCrc3;sZZRm\CCocn;tJGNNVCCyYYTp[Y51KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBHSUOVIHHuZYx6e2m|LDDFR|UxKD1iMD6xOkDPxE1w MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ5M{ezPUc,OjR4N{O3N|k9N2F-
K562 MoH0R5l1d3SxeHnjbZR6KGG|c3H5 MY[3NkBpenN? NWPuT3JiS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4heGGlbHn0ZZhmdC2{ZYPpd5RidnRiS{W2NkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMD6yOkDPxE1w NGLxUI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{ME[wOVY2Pid-MkC2NFU3PTZ:L3G+
CLL3 NFLsV5VCdnSrdIXtc5Ih[XO|YYm= MXS0PEBpenN? NITMV4lCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBEVEx|IHPlcIx{KGm|b3zheIVlKG[{b32gR2xNKHCjdHnlcpQh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JGZCS1NiYX7hcJl{cXNuIFXDOVAhRSByLkO1JO69VS5? Mn7pQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4N{O3N|koRjJ2NkezO|M6RC:jPh?=
CLL4 MUDBcpRqfHWvb4KgZZN{[Xl? MkiwOFghcHK| NGLUNWtCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBEVEx2IHPlcIx{KGm|b3zheIVlKG[{b32gR2xNKHCjdHnlcpQh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JGZCS1NiYX7hcJl{cXNuIFXDOVAhRSByLk[0JO69VS5? NY\C[2ZbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2O|M4OzlpPkK0Olc{PzN7PD;hQi=>
CEM-DNR-B Mn3zR5l1d3SxeHnjbZR6KGG|c3H5 M2\nOFczKGi{cx?= M1jaRmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNGVS2GTmKtRkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMT6wNUDPxE1w MnfnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB4MEW2OVYoRjJyNkC1OlU3RC:jPh?=
primary CLL NE[wVmxEgXSxdH;4bYNqfHliYYPzZZk> NYm3RYh2S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4heHKrbXHyfUBEVExiY3XscJMtKEyGNUCgQUAyNjFizszNMi=> NFrkOlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG0PFM6Oid-MkWxOFg{QTJ:L3G+
CLL6 NHK3TWdCdnSrdIXtc5Ih[XO|YYm= NIK3bYQ1QCCqcoO= M12w[mFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JGNNVDZiY3XscJMhcXOxbHH0[YQh\nKxbTDDUGwheGG2aXXueEBie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjC0PEBpenNiYomgSmFEWyCjbnHsfZNqeyxiRVO1NEA:KDFwNjFOwG0v NFLBOpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[3N|c{QSd-MkS2O|M4Ozl:L3G+
CLL2 MUXBcpRqfHWvb4KgZZN{[Xl? MmLROFghcHK| M3fsTmFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JGNNVDJiY3XscJMhcXOxbHH0[YQh\nKxbTDDUGwheGG2aXXueEBie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjC0PEBpenNiYomgSmFEWyCjbnHsfZNqeyxiRVO1NEA:KDJwNk[g{txONg>? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ5M{ezPUc,OjR4N{O3N|k9N2F-
HCT116 MkPFR5l1d3SxeHnjbZR6KGG|c3H5 NXLwZZl2PzJiaILz NUG2NYFYS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWMUG2JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCVUlKgZZN{[XluIFnDOVAhRSB4Lk[g{txONg>? MoniQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2NkKyO|coRjJ3NE[yNlc4RC:jPh?=
PBMC MVjDfZRwfG:6aXPpeJkh[XO|YYm= NYPQUoliPDhiaILz NFe4ZW5EgXSxdH;4bYNqfHliYXfhbY5{fCCyYYTp[Y51KFCETVOgZYZ1\XJiNEigbJJ{KGK7IFPlcIxVcXS{ZT3CcJVmKGG|c3H5JIlvKHC{ZYPlcoMhd2ZibX;1d4UhVTJzMFK0JINmdGy|LDDJR|UxKD1iMUCg{txONg>? NYDyNWFiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OlI1OTdpPkK1OVYzPDF5PD;hQi=>
CHO NHXjVoVHfW6ldHnvckBie3OjeR?= M4rDVmJqdmSrbnegZYZncW6rdImg[IV1\XKvaX7l[EBjgSCmaYPwcIFk\W2nboSgc4Yhe3CnY3nmbYMh[mmwZHnu[{Bw\iCdMUK1TX1PNSh2LXHtbY5wNTNvaX;kc5Bp\W6ndHj5cEku[WSnbn;zbY5mKGmwIH3lcYJz[W6nczDv[kBEUE9iY3XscJMhe3SjYnz5JJRz[W6|ZnXjeIVlKHerdHigeIhmKHKjdDDh[IVvd3OrbnWgRVMhemWlZYD0c5ItKEurIE2gNVAvPCEQvF2u NIm3TnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi95N{C3N|IxLz55N{C3N|IxRC:jPh?=
JVM2 NYDUWllTSW62aYT1cY9zKGG|c3H5 Mo\FRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSm\NNkBk\WyuczDhd5Nme3OnZDDhd{Bk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliRlHDV{BidmGueYPpd{whTUN3MDC9JFExNjRizszNMi=> NWXnV4ljRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2O|M4OzlpPkK0Olc{PzN7PD;hQi=>
HeLa MUPBcpRqfHWvb4KgZZN{[Xl? MUjBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZVzhJINmdGy|IHHzd4V{e2WmIHHzJINmdGxidnnhZoltcXS7IHL5JG1VXCCjc4PhfUwhTUN3MDC9JFE3KM7:TT6= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ5M{ezPUc,OjR4N{O3N|k9N2F-
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Saos-2 MWrxTHRUKGG|c3H5 MXHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW2Gxcz2yJINmdGy| Mki3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
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BT-37 NF35e3ByUFSVIHHzd4F6 NGL3N|VyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBDXC1|NzDj[Yxtew>? Mom5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
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LAN-5 NUPzfot7eUiWUzDhd5NigQ>? NGXKS4dyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTFHOMVUh[2WubIO= MmHiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
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NB1643 MmLXdWhVWyCjc4PhfS=> NXzVTZNoeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy| MmTPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC M2fuUJFJXFNiYYPzZZk> NWK2dHpZeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= M1nWflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SJ-GBM2 MlS4dWhVWyCjc4PhfS=> NVjJOWt7eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
TC32 NV7CXZFqeUiWUzDhd5NigQ>? MU\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDUR|MzKGOnbHzz NXHxRo1mRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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Saos-2 M3nQ[ZFJXFNiYYPzZZk> NGPjNlhyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBU[W:|LUKgZ4VtdHN? MmfXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
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RD MlzFdWhVWyCjc4PhfS=> M2rXPJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KE:{dHjv[49v[WxiM1Sgeoli[mmuaYT5JJNkemWnbjDmc5IhWkRiY3XscJM> M{fHPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=

他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

アッセイ
Methods Test Index PMID
Western blot
procaspase-9 / procaspase-3; 

PubMed: 27223263     


Procaspase-9 and procaspase-3 were analyzed by Western blot in CLL cells from three patients after a 48 h-incubation in the absence or presence of 3 μM aphidicolin (APC) with or without fludarabine at 0.3 and 1 μM. β-Actin served as a loading control.

p-p53 / p53; 

PubMed: 27223263     


(A–B) CLL cells from 3 different patients were incubated for 24 h with fludarabine at the indicated concentrations in the absence or presence of 3 μM aphidicolin (APC). Phosphorylation of p53 at Ser-15 and total p53 were analyzed by Western blot. Data sho䲧疝Ỵ疞㧀疜膉痘 瘿�෋ᾰƌ෋à 㺣痖帉痖Ѐ瑖堘𢡄빢᎒෋à鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒

STAT1; 

PubMed: 26677135     


Representative western blot analysis of STAT1 in RAW 264.7 cells treated with STAT1 inhibitor fludarabine (Flu) for 24 h.

27223263 26677135
Immunofluorescence
α-SMA / Vimentin; 

PubMed: 28322315     


FLU (50 μM) reduces the expression of α-SMA and Vimentin protein in primary mouse activated HSCs (Hepatic Stellate Cells).

28322315
Growth inhibition assay
Cell viability ; 

PubMed: 24956101     


CLL cells RPMI/0.1% FBS were cultured on 0.5% BSA or 150 nM MMP-9 for 1 h prior to adding the indicated concentrations of fludarabine (Fluda). After 48 h (Fluda) cell viability was determined by flow cytometry using FITC-Annexin V and PI.

24956101
体内試験 Tumors treated with PBS grow rapidly to approx-imately 10-fold their initial volume in 25 day, whereas, the tumors in the Fludarabine at 40 mg/kg increase less than 5-fold. A significant antitumor effect of 40 mg/kg Fludarabine on RPMI8226 tumor growth is demonstrated. RPMI8226 tumors treated with 40 mg/kg Fludarabine at day 10 increase apoptotic nuclei. Fludarabine is effective in suppressing RPMI8226 myeloma xenografts in SCID mice. [1]

お薦めの試験操作(参考用のみ)

細胞試験:

[1]

- 合併
  • 細胞株: Dexamethasone-sensitive (MM.1S) and -resistant (MM.1R) human MM cell lines, RPMI8226 and U266 cell lines
  • 濃度: 2 μg/mL
  • 反応時間: 24 hours
  • 実験の流れ:

    After treated with Fludarabine or control, dexamethasone-sensitive (MM.1S) and -resistant (MM.1R) human MM cell lines, RPMI8226 and U266 cell lines (5 × 105 cells) are washed twice in phosphate-buffered saline (PBS) and fixed with 70% ice-cold ethanol, then centrifuged and suspended in PBS containing 100 μg/mL RNase A. After incubated for 30 minutes at 37 ºC, samples are resuspended in 25 μg/mL propidium iodide. Flow cytometry is performed on a FACSCalibur automated system. Apoptosis is determined by Annexin V-FITC apoptosis detection kit, according to the manufacturer's instructions. For TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling) assay, cells are analyzed by flow cytometry using the in situ cell death detection kit.


    (参考用のみ)
動物試験:

[1]

- 合併
  • 動物モデル: Severe combined immunodeficient (SCID) mice bearing RPMI 8226 cells
  • 投薬量: 40 mg/kg
  • 投与方法: Administered via i.p.
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 57 mg/mL (199.83 mM)
Water Insoluble
Ethanol Insoluble
体内 左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O
混合させたのち直ちに使用することを推奨します。
8 mg/mL

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。

化学情報

分子量 285.23
化学式

C10H12FN5O4

CAS No. 21679-14-1
Storage powder
in solvent
別名 FaraA, Fludarabinum
Smiles C1=NC2=C(N=C(N=C2N1C3C(C(C(O3)CO)O)O)F)N

投与溶媒組成計算器(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)
投与量 mg/kg 動物平均体重 g 投与体積(動物毎) ul 動物数
ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)
% DMSO % % Tween 80 % ddH2O
計算リセット

便利ツール

モル濃度計算器

モル濃度計算器

求めたい質量、体積または濃度を計算してください。

質量 (mg) = 濃度 (mM) x 体積 (mL) x 分子量 (g/mol)

モル濃度計算器方程式

  • 質量
    濃度
    体積
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備するために必要な希釈率を計算してください。Selleck希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 入力 出力 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

質量 濃度 体積 分子量

臨床試験

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04526795 Recruiting Drug: Cytarabine|Drug: Fludarabine|Drug: Pegcrisantaspase Blast Phase Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent Acute Biphenotypic Leukemia|Recurrent Acute Lymphoblastic Leukemia|Recurrent Acute Myeloid Leukemia|Recurrent Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent T-Cell Prolymphocytic Leukemia|Refractory Acute Biphenotypic Leukemia|Refractory Acute Lymphoblastic Leukemia|Refractory Acute Myeloid Leukemia|Refractory Chronic Myelogenous Leukemia BCR-ABL1 Positive|Refractory T-Cell Prolymphocytic Leukemia M.D. Anderson Cancer Center|National Cancer Institute (NCI) April 9 2021 Phase 1

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

  • * 必須

よくある質問(FAQ)

  • 質問1:

    how to re-suspend and deliver the inhibitor for in vivo experiments?

  • 回答:

    For S1491, Fludarabine, we tested a vehicle: 30% Propylene glycol, 5% Tween 80, 65% D5W that you can resuspend the compound in at up to 30mg/ml. It's a suspension and can only be given via oral gavage.

STATシグナル伝達経路

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細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID