Nordihydroguaiaretic acid (NDGA)

Nordihydroguaiaretic acid (NDGA) is a phenolic antioxidant found in the leaves and twigs of the evergreen desert shrub, Larrea tridentata (Sesse and Moc. ex DC) Coville (creosote bush). It is a recognized inhibitor of lipoxygenase (LOX) and has antioxidant and free radical scavenging properties. Nordihydroguaiaretic acid (NDGA) is a cytotoxic insulin-like growth factor-I receptor (IGF-1R)/HER2 inhibitor and induces apoptosis. Nordihydroguaiaretic acid (NDGA) inhibits p300 and activates autophagy. Nordihydroguaiaretic acid (NDGA) protects cells from ferroptosis.

Nordihydroguaiaretic acid (NDGA)化学構造

CAS No. 500-38-9

サイズ 価格(税別) 在庫状況
JPY 23100 国内在庫あり
JPY 216600 国内在庫なし(納期7~10日)

代表番号: 045-509-1970|電子メール:[email protected]
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Nordihydroguaiaretic acid (NDGA)関連製品

Lipoxygenase阻害剤の選択性比較

生物活性

製品説明 Nordihydroguaiaretic acid (NDGA) is a phenolic antioxidant found in the leaves and twigs of the evergreen desert shrub, Larrea tridentata (Sesse and Moc. ex DC) Coville (creosote bush). It is a recognized inhibitor of lipoxygenase (LOX) and has antioxidant and free radical scavenging properties. Nordihydroguaiaretic acid (NDGA) is a cytotoxic insulin-like growth factor-I receptor (IGF-1R)/HER2 inhibitor and induces apoptosis. Nordihydroguaiaretic acid (NDGA) inhibits p300 and activates autophagy. Nordihydroguaiaretic acid (NDGA) protects cells from ferroptosis.
Targets
lipoxygenase [1] Ferroptosis [6] IGF-1R [4] HER2 [4] p300 [5]
In Vitro
In vitro

NDGA has been proven to selectively inhibit arachidonic acid 5-lipoxygenase activity, which reduces leukotriene and prostaglandin synthesis, thus leading to a reduction of inflammatory pathways. NDGA also has profound effects on the secretory pathway, reflected in its ability to block production of leukotriene B4, degranulation, phagocytosis, and the respiratory burst by exerting effects on the mitochondria and nonspecifically inhibiting NADPH oxidase and protein kinase C. NDGA has also been shown to block protein transport from the endoplasmic reticulum (ER) to the Golgi complex, induce the redistribution of Golgi proteins into the ER and affect levels of intracellular calcium. Furthermore, NDGA has been shown to disrupt the actincytoskeleton and exert effects on cell adhesion and also to directly inhibit activationof two receptor tyrosine kinases (RTKs), the Insulin-like growth factor-1 receptor and the c-erbB2/HER2/neu receptor, that results in decreased cellular proliferation. NDGA selectively inhibits platelet-derived growth factor (PDGF)-stimulated DNA synthesis in Swiss 3T3 cells, diploid murine cells and rat and human fibroblasts. NDGA is a bioactive natural product which is able to crosslink collagen. NDGA cross-linking may provide a viable approach to stabilizing collagenous materials for use in repair of ruptured, lacerated or surgically transected tendons, as well as other biomaterial constructs for surgical repair of musculoskeletal injuries and disease[1].

細胞実験 細胞株 PC3 cells
濃度 10-50 μM
反応時間 16 h
実験の流れ

Cytotoxicity tests are carried out using WST-1, a fluorescent cell proliferation reagent. The assay is based on cleavage of the tetrazolium salt WST-1 by active mitochondria to produce a soluble colored formazan salt. The cells are plated at 1 × l04 in 96-well microtiter plates. Twenty-four hours after plating, at 70% confluence the growth medium is removed and replaced with the test solutions (100 ìl). After 16-hr exposure, the reaction medium (in the presence or absence of 10-50 μM NDGA) is removed, the cells are washed twice with culture medium, then 100 μl culture medium and 10 μl WST-1 are added to each well. The cells are incubated for 2 hrs at 37 °C in a humidified atmosphere with 5% CO2, then the microplate is thoroughly shaken for 1 min and the absorbance is measured at 450 nm using a microtiter reader.

In Vivo
In Vivo

Adding 0.1% NDGA to the drinking water of athymic mice bearing non-small cell lung cancer tumors significantly inhibits tumor growth compared with control mice. In addition, NDGA has not only been shown to suppress breast cancer cell growth, it has a synergistic effect with retinoic acid on the inhibition of mammary tumor cell transformation and proliferation. Preliminary in vivo studies have revealed that NDGA suppresses tumor growth by inhibiting metabolic enzymes as well as RTK phosphorylation, which is overexpressed in certain cancer cells. NDGA has also been proven to be a potent anti-ischemia-reperfusion injury agent in vitro and in animal models through different antioxidant pathways. It has been identified as a compound capable of inducing glutamate uptake and upregulation of expression levels and activity of the glutamate transporter EAAT2 (GLT-1) in mice[1].

動物実験 動物モデル Male Swiss albino mice
投与量 1 and 2 mg/animal/day
投与経路 oral

化学情報

分子量 302.36 化学式

C18H22O4

CAS No. 500-38-9 SDF Download Nordihydroguaiaretic acid (NDGA) SDFをダウンロードする
Smiles CC(CC1=CC(=C(C=C1)O)O)C(C)CC2=CC(=C(C=C2)O)O
保管 3 years -20°C powder (seal)

In vitro
Batch:

DMSO : 60 mg/mL ( (198.43 mM); 吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Ethanol : 60 mg/mL

Water : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量 濃度 体積 分子量

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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