Curcumin

製品コードS1848 別名:Diferuloylmethane

Curcumin化学構造

分子量(MW):368.38

Curcumin is the principal curcuminoid of the popular Indian spice turmeric, which is a member of the ginger family (Zingiberaceae). It is an inhibitor of p300 histone acetylatransferase(IC50~25 μM) and Histone deacetylase; activates Nrf2 pathway and supresses the activation of transcription factor NF-κB.

サイズ 価格(税別)  
JPY 21580.00
JPY 16102.00

カスタマーフィードバック(4)

  • Effect of NC1, NC1-CUR, NC2 and NC2-CUR on SH-SY5Y cell viability. The cells were treated for 24 h with different dilutions(1:1, 1:2 and 1:4) of empty nanocapsules (NC1 and NC2) and nanocapsules containing curcumin (NC1-CUR and NC2-CUR) followed by measurement of cell viability by MTT reduction assay (panel A) and cell toxicity by LDH release assay (panel B). Data after normalization to vehicle-treated cells (100%, MTT assay) or to total LDH release (TritonX100-treated cells, 100%) are presented as a mean±SEM from 3-8 independent experiments with five replicates. *p < 0.05, **p < 0.01 and ***p<0.001 versus vehicle-treated cells; &&&p<0.001 NC1 versus NC1-CUR.

    Nanotechnology, 2016, 27(35):355101. Curcumin purchased from Selleck.

    Oncol Res 2014 21(3), 137-44. Curcumin purchased from Selleck.

  • Oncol Res 2014 21(3), 137-44. Curcumin purchased from Selleck.

    Inhibition of [3H]uridine uptake in MIA PaCa-2 (A & B) and PANC-1 (C) cells by curcumin (CUR) and A13. Results are normalised to the disintegrations/min (dpm) of DMSO-only control and are means ±S.D. of n =3 independent experiments done in triplicates. ***P<0.001 significantly different from DMSO-control, calculated using one-way ANOVA and Dunnett's post-hoc test. NBMPR-Nitrobenzylthioinosine. Conc-concentration.

    Eur J Pharmacol, 2017, 803:167-173. Curcumin purchased from Selleck.

製品安全説明書

Histone Acetyltransferase阻害剤の選択性比較

生物活性

製品説明 Curcumin is the principal curcuminoid of the popular Indian spice turmeric, which is a member of the ginger family (Zingiberaceae). It is an inhibitor of p300 histone acetylatransferase(IC50~25 μM) and Histone deacetylase; activates Nrf2 pathway and supresses the activation of transcription factor NF-κB.
ターゲット
Nrf2 [1]
(Cell-free assay)
HDAC [7]
(Cell-free assay)
NF-κB [8]
(Cell-free assay)
p300 histone acetylatransferase [6]
(Cell-free assay)
~25 μM
体外試験

Curcumin induces the expression of forkhead box protein O1 (FOXO1) through activation of extracellular signal-regulated kinase 1/2 signaling. Curcumin inhibits cell proliferation, which was associated with upregulation of the cyclin-dependent kinase inhibitors, p27 and p21, and downregulation of cyclin D1[2]. Curcumin induces endoplasmic reticulum (ER) stress and mitochondrial dysfunction as evidenced by up-regulation of CCAAT/enhancer binding protein homologous protein (CHOP), phosphorylation of JNK and down-regulation of SERCA2ATPase, release of cytochrome c, decrease of Bcl-2 and reduction of mitochondrial membrane potential in both AGS and HT-29 cells[3].

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human KBM5 cells M{K0dHBzd2yrZnXyZZRqd25iYYPzZZk> M1XJbFczKGh? MWfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEuETUWgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0{Njh2IN88US=> Mk\pNlQzQDByNkm=
human Jurkat cells MV;Qdo9tcW[ncnH0bY9vKGG|c3H5 NYDpPFQ6PzJiaB?= NV:0PHhOSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDKeZJs[XRiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME20MlI6KM7:TR?= NVO0UolNOjR{OECwOlk>
human U266 cells NH23fIFRem:uaX\ldoF1cW:wIHHzd4F6 M3v5N|czKGh? M4Sxc2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVUK2OkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTdwNUeg{txO MVyyOFI5ODB4OR?=
human A549 cells M{KwOnBzd2yrZnXyZZRqd25iYYPzZZk> MYK3NkBp NV;hcoZKSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUeuN{DPxE1? NWnhV4VFOjR{OECwOlk>
human MCF7 cells MVnDfZRwfG:6aXRCpIF{e2G7 NVXkUZNzOjRiaB?= M2S1Z2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2zNk4zKM7:TR?= MonLNlYyPzR3NUW=
human SKOV3 cells M3PZOmN6fG:2b4jpZ:Kh[XO|YYm= MVGyOEBp MVfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT29XOyClZXzsd{Bi\nSncjCyOEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUSzMlY{KM7:TR?= Mkn0NlYyPzR3NUW=

他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

体内試験 Chronic treatment with curcumin significantly reverses the CMS-induced behavioral abnormalities in stressed rats. Additionally, curcumin effectively inhibits cytokine gene expression at both the mRNA and the protein level and reduces the activation of NF-κB[4].

お薦めの試験操作(参考用のみ)

細胞試験:

[5]

+ 展開
  • 細胞株: murine melanoma cell subline(B16-R)
  • 濃度: 0-100 μM
  • 反応時間: 24-48 h
  • 実験の流れ:

    1×104 B16-R cells are cultivated as monolayer culture for 12 hr. They were then incubated in 200 μL of RPMI, 10% FBS containing curcumin at final concentrations from 1–100 μM in 96-multiwell plates for 24-48 hr. After these incubations, cells are washed twice in PBS and 500 μl of fresh culture medium containing MTT (0.3 mg/mL) are added for colorimetric assay.


    (参考用のみ)
動物試験:

[5]

+ 展開
  • 動物モデル: Female B6D2F1 mice (6-8 weeks old)
  • 製剤: Dissolved in DMSO and diluted with 0.85% NaCl
  • 投薬量: 25 mg/kg
  • 投与方法: i.p.
    (参考用のみ)

溶解度 (25°C)

体外 DMSO 73 mg/mL (198.16 mM)
Water Insoluble
Ethanol Insoluble
体内 左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
10% DMSO+50% PEG 300+ddH2O
混合させたのち直ちに使用することを推奨します。
40mg/mL

* 溶解度測定はSelleck技術部門によって行われており、その他文献に示されている溶解度と差異がある可能性がありますが、同一ロットの生産工程で起きる正常な現象ですからご安心ください。

化学情報

分子量 368.38
化学式

C21H20O6

CAS No. 458-37-7
保管
in solvent
別名 Diferuloylmethane

便利ツール

モル濃度計算器

モル濃度計算器

求めたい質量、体積または濃度を計算してください。

質量 (g) = 濃度 (mol/L) x 体積 (L) x 分子量 (g/mol)

モル濃度計算器方程式

  • 質量
    濃度
    体積
    分子量

*貯蔵液を準備するとき、常に、オンであるとわかる製品のバッチに特有の分子量を使って、を通してラベルとMSDS/COA(製品ページで利用可能な)。

希釈計算器

希釈計算器

貯蔵液を準備するために必要な希釈率を計算してください。Selleck希釈計算器は、以下の方程式に基づきます:

開始濃度 x 開始体積 = 最終濃度 x 最終体積

希釈の計算式

この方程式は、一般に略語を使われます:C1V1 = C2V2 ( 入力 出力 )

  • C1
    V1
    C2
    V2

常に貯蔵液を準備するとき、小びんラベルとMSDS/COA(オンラインで利用できる)で見つかる製品のバッチに特有の分子量を使ってください。

連続希釈計算器方程式

  • 連続希釈剤

  • 計算結果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量計算器

分子量计算器

そのモル質量と元素組成を計算するために、合成物の化学式を入力してください:

総分子量:g/mol

チップス: 化学式は大文字と小文字の区別ができます。C10H16N2O2 c10h16n2o2

モル濃度計算器

質量 濃度 体積 分子量

臨床試験

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02277223 Not yet recruiting Ulcerative Colitis Schneider Children''s Medical Center Israel January 1 2019 Phase 3
NCT03598309 Not yet recruiting Lung Diseases|Lung Cancer Protection Against H. Lee Moffitt Cancer Center and Research Institute|James and Esther King Biomedical Research Program November 2018 Phase 2
NCT03542240 Recruiting Obesity|High Blood Pressure|High Cholesterol|Type2 Diabetes|Heart Diseases Virginia Commonwealth University September 19 2018 Not Applicable
NCT03568513 Recruiting Irritable Bowel Syndrome Medical College of Wisconsin|Clinical & Translational Science Institute of Southeast Wisconsin August 1 2018 Not Applicable
NCT03262363 Not yet recruiting Chronic Kidney Diseases|Diabetes Mellitus Type 2|Polymorphism Unidad de Investigacion Medica en Enfermedades Renales August 1 2018 Phase 2|Phase 3
NCT03534024 Recruiting Metabolic Syndrome National Nutrition and Food Technology Institute August 30 2018 Not Applicable

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

  • * 必須

Histone Acetyltransferaseシグナル伝達経路

Tags: Curcuminを買う | Curcumin ic50 | Curcumin供給者 | Curcuminを購入する | Curcumin費用 | Curcumin生産者 | オーダーCurcumin | Curcumin化学構造 | Curcumin分子量 | Curcumin代理店
×
細胞株 試験類型 濃度 培養時間 溶剤類型 活性叙述 PMID