Curcumin

別名：Diferuloylmethane, Natural Yellow 3, Turmeric yellow

製品コード：S1848 純度：99.94%

Curcumin (Diferuloylmethane, Natural Yellow 3, Turmeric yellow) is the principal curcuminoid of the popular Indian spice turmeric, which is a member of the ginger family (Zingiberaceae). It is an inhibitor of p300 histone acetylatransferase（IC50~25 μM）and Histone deacetylase (HDAC); activates Nrf2 pathway and supresses the activation of NF-κB. Curcumin induces mitophagy, autophagy, apoptosis, and cell cycle arrest with antitumor activity. Curcumin reduces renal damage associated with rhabdomyolysis by decreasing ferroptosis-mediated cell death. Curcumin exhibits anti-infective properties against various human pathogens like the influenza virus, hepatitis C virus, HIV and so on.

CAS No. 458-37-7

サイズ	価格(税別)	在庫状況
10mM (1mL in DMSO)	JPY 29500	国内在庫あり
50mg	JPY 22000	国内在庫あり
1g	JPY 70500	国内在庫なし(納期7~10日)

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

文献中Selleckの製品使用例（42）

製品安全説明書

現在のバッチを見る：純度： 99.94%

99.94

Curcumin関連製品

関連ターゲット	HAT MYST GNAT p300/CBP	もっと見る
関連製品	C646 Anacardic Acid MG149 A-485 Remodelin hydrobromide WM-1119 YF-2 CPTH2	もっと見る
関連化合物ライブラリー	Kinase Inhibitor Library FDA-approved Drug Library Natural Product Library Bioactive Compound Library-I Bioactive Compound Library-Ⅱ	もっと見る

シグナル伝達経路

Histone Acetyltransferaseシグナル伝達経路

Histone Acetyltransferase阻害剤の選択性比較

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	活性情報	PMID
UMUC3	Cell viability assay	0.1 or 0.4 μg/ml	24, 48, and 73 h	Curcumin alone, added at a concentration from 0.1 to 0.4 μg/ml, did not alter cell growth of RT112 cells, slightly reduced UMUC3 cell growth at 0.4 μg/ml, and only moderately suppressed growth of TCCSUP at 0.2 and 0.5 μg/ml, each compared to respective controls	30984278
RT112	Cell viability assay	0.1 or 0.4 μg/ml	24, 48, and 72 h	Curcumin alone, added at a concentration from 0.1 to 0.4 μg/ml, did not alter cell growth of RT112 cells, slightly reduced UMUC3 cell growth at 0.4 μg/ml, and only moderately suppressed growth of TCCSUP at 0.2 and 0.4 μg/ml, each compared to respective controls	30984278
U87	Cell viability assay	0, 10, 20, 30, 40, and 50 µM/L	24 hours	At concentrations higher than 20 µM/L, cell viabilities steeply declined	31062527
HEL cells	Cell viability assay	0-30 µmol/L	24/48 hours	The viability of the HEL cells exposed to curcumin was significantly lower compared to control cells even at low concentrations.	31033209
TCCSUP	Cell viability assay	0.1 or 0.4 μg/ml	24, 48, and 74 h	Curcumin alone, added at a concentration from 0.1 to 0.4 μg/ml, did not alter cell growth of RT112 cells, slightly reduced UMUC3 cell growth at 0.4 μg/ml, and only moderately suppressed growth of TCCSUP at 0.2 and 0.6 μg/ml, each compared to respective controls	30984278
A549	Apoptosis assay	2.1 to 4.2 uM	24 hrs	Induction of apoptosis in human A549 cells at 2.1 to 4.2 uM after 24 hrs by annexin V-FITC/propidium iodide staining-based flow cytometry	26891099
A549/CDDP	Apoptosis assay	0.6 to 1.2 uM	24 hrs	Induction of apoptosis in human A549/CDDP cells at 0.6 to 1.2 uM after 24 hrs by annexin V-FITC/propidium iodide staining-based flow cytometry	26891099
SH-SY5Y	Function assay	1 to 5 uM	24 hrs	Antioxidant activity against t-BHP-induced oxidative stress in human SH-SY5Y cells assessed as inhibition of ROS production at 1 to 5 uM after 24 hrs by DCFH-DA probe based flow cytometric analysis	27856236
K562	Function assay	20 to 30 uM	48 hrs	Down regulation of p-gp expression in doxorubicin resistant human K562 cells at 20 to 30 uM measured after 48 hrs by Western blot method	27908756
K562	Cell cycle assay	20 uM	48 hrs	Cell cycle arrest in doxorubicin resistant human K562 cells assessed as decrease in G0/G1 phase at 20 uM measured after 48 hrs by propidium iodide staining based flow cytometry	27908756
K562	Cell cycle assay	20 uM	48 hrs	Cell cycle arrest in doxorubicin resistant human K562 cells assessed as increase in G2/M phase at 20 uM measured after 48 hrs by propidium iodide staining based flow cytometry	27908756
K562	Function assay	20 to 40 uM	48 hrs	Up regulation of p53 expression in doxorubicin resistant human K562 cells at 20 to 40 uM measured after 48 hrs by Western blot method	27908756
K562	Cell cycle assay	20 uM	48 hrs	Cell cycle arrest in doxorubicin resistant human K562 cells assessed as decrease in S phase at 20 uM measured after 48 hrs by propidium iodide staining based flow cytometry	27908756
K562	Cell cycle assay	30 to 40 uM	48 hrs	Cell cycle arrest in doxorubicin resistant human K562 cells assessed as accumulation at G0/G1 phase at 30 to 40 uM measured after 48 hrs by propidium iodide staining based flow cytometry	27908756
K562	Cell cycle assay	30 to 40 uM	48 hrs	Cell cycle arrest in doxorubicin resistant human K562 cells assessed as decrease in G2/M phase at 30 to 40 uM measured after 48 hrs by propidium iodide staining based flow cytometry	27908756
K562	Function assay	20 to 40 uM	48 hrs	Down regulation of cyclin B1 expression in doxorubicin resistant human K562 cells at 20 to 40 uM measured after 48 hrs by Western blot method	27908756
K562	Apoptosis assay	20 to 40 uM	48 hrs	Induction of apoptosis in doxorubicin resistant human K562 cells decrease in pro-caspase 3 level at 20 to 40 uM measured after 48 hrs by Western blot method	27908756
K562	Apoptosis assay	20 to 40 uM	48 hrs	Induction of apoptosis in doxorubicin resistant human K562 cells assessed as increase in PARP-1 cleavage at 20 to 40 uM measured after 48 hrs by Western blot method	27908756
PC12	Neuroprotective assay	0.1 to 10 uM	24 hrs	Neuroprotective activity against H202-induced toxicity in rat PC12 cells assessed as increase in cell viability at 0.1 to 10 uM after 24 hrs by MTT assay	28302511
PC12	Neuroprotective assay	0.1 to 10 uM	24 hrs	Neuroprotective activity against Abeta42-induced toxicity in rat PC12 cells assessed as increase in cell viability at 0.1 to 10 uM after 24 hrs by MTT assay	28302511
cells at	Growth inhibition assay	30 uM	48 hrs	Growth inhibition of human Hep3B cells at 30 uM after 48 hrs by MTT assay	28342400
HepG2	Function assay	5 uM	24 hrs	Activation of Nrf2 in human HepG2 cells assessed as cytoprotection against t-BHP-induced oxidative cell death at 5 uM incubated for 24 hrs followed by compound removal with subsequent incubation with 900 uM of t-BHP for 6 hrs by MTT assay	28399452
HCT116	Function assay	500 uM	48 hrs	Induction of glutathione oxidation in human HCT116 cells assessed as increase in GSSG/GSH ratio at 500 uM in presence of NADPH after 48 hrs by Ellman's method	28716495
BGC823	Growth inhibition assay	20 uM	72 hrs	Growth inhibition of human BGC823 cells at 20 uM after 72 hrs by MTT assay	28881286
SGC7901	Growth inhibition assay	20 uM	72 hrs	Growth inhibition of human SGC7901 cells at 20 uM after 72 hrs by MTT assay	28881286
MFC	Growth inhibition assay	20 uM	72 hrs	Growth inhibition of MFC cells at 20 uM after 72 hrs by MTT assay	28881286
NCI-H460	Growth inhibition assay	5 to 10 uM	24 to 72 hrs	Growth inhibition of human NCI-H460 cells assessed as reduction in cell viability at 5 to 10 uM incubated for 24 to 72 hrs by MTT assay	29288946
NCI-H460	Growth inhibition assay	20 uM	18 hrs	Anti-growth activity against human NCI-H460 cells assessed as reduction in colony formation at 20 uM after 18 hrs by crystal violet-based colony forming assay	29288946
NCI-H460	Antimigratory assay	20 uM	48 hrs	Anti-migratory activity against human NCI-H460 cells at 20 uM incubated for 48 hrs by wound healing assay	29288946
NCI-H460	Apoptosis assay	15 mg/kg	15 days	Induction of apoptosis in tumor of human NCI-H460 cells xenografted in BALB/C nu nude mouse assessed as downregulation of bcl2 expression at 15 mg/kg, ip administered everyday for 15 days by Western blot analysis	29288946
HT22	Cytoprotection assay	1 uM	24 hrs	Cytoprotection against glutamate-induced apoptosis in mouse HT22 cells assessed as decrease in Bax/Bcl2 ratio at 1 uM after 24 hrs by RT-PCR method	30031653
HT22	Cytoprotection assay	1 uM	24 hrs	Cytoprotection against glutamate-induced oxidative stress in mouse HT22 cells assessed as decrease in iNOS mRNA level at 1 uM after 24 hrs by RT-PCR method	30031653
HT22	Cytoprotection assay	1 uM	24 hrs	Cytoprotection against glutamate-induced excitotoxicity in mouse HT22 cells assessed as increase in cell viability at 1 uM after 24 hrs by optical microscopic assay	30031653
HT22	Cytoprotection assay	1 uM	24 hrs	Cytoprotection against glutamate-induced excitotoxicity in mouse HT22 cells assessed as decrease in glutamate-induced cell cycle arrest at sub-G1 phase at 1 uM after 24 hrs by propidium iodide staining based flow cytometry	30031653
HT22	Cytoprotection assay	1 uM	24 hrs	Cytoprotection against glutamate-induced excitotoxicity in mouse HT22 cells assessed as increase in cell proliferation at 1 uM after 24 hrs by MTT assay	30031653
RAW264.7	Function assay	10 uM	0.5 hrs	Inhibition of MAPK/NFkappaB in ICR mouse RAW264.7 cells assessed as reduction in LPS-induced TNFalpha production at 10 uM pretreated for 0.5 hrs followed by LPS challenge and measured after 24 hrs by ELISA relative to control	30780088
RAW264.7	Function assay	10 uM	0.5 hrs	Inhibition of LPS-induced p38 MAPK phosphorylation in ICR mouse RAW264.7 cells at 10 uM preincubated for 0.5 hrs followed by LPS challenge and measured after 30 mins by immunoblotting analysis	30780088
RAW264.7	Function assay	10 uM	0.5 hrs	Inhibition of LPS-induced ERK phosphorylation in ICR mouse RAW264.7 cells at 10 uM preincubated for 0.5 hrs followed by LPS challenge and measured after 30 mins by immunoblotting analysis	30780088
RAW264.7	Function assay	10 uM	0.5 hrs	Inhibition of NFkappaB/MAPK signaling in LPS-induced mouse RAW264.7 cells assessed as down regulation of IkappaB expression at 10 uM preincubated for 0.5 hrs followed by LPS-challenge and measured after 30 mins by immunoblotting analysis	30780088
RAW264.7	Function assay	10 uM	0.5 hrs	Inhibition of MAPK/NFkappaB in ICR mouse RAW264.7 cells assessed as reduction in LPS-induced IL6 production at 10 uM pretreated for 0.5 hrs followed by LPS challenge and measured after 24 hrs by ELISA relative to control	30780088
Raw246.7	Cell viability assay	1, 5, 10, 50, 100, 200, and 500 g/mL			31007701
RAW264.7	Antiinflammatory assay	20 uM		Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL-6 production at 20 uM by ELISA	28284806
RAW264.7	Antiinflammatory assay	20 uM		Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production at 20 uM by ELISA	28284806
3T3L1	Function assay	10 ng/ml		Inhibition of TNFalpha-induced NFkappaB activation (unknown origin) expressed in mouse 3T3L1 cells at 10 ng/ml by luciferase reporter gene assay	ChEMBL
KBM5	Antiproliferative assay		72 hrs	Antiproliferative activity against human KBM5 cells after 72 hrs by MTT assay, IC50 = 3.84 μM.	24280069
Jurkat	Antiproliferative assay		72 hrs	Antiproliferative activity against human Jurkat cells after 72 hrs by MTT assay, IC50 = 4.29 μM.	24280069
U266	Antiproliferative assay		72 hrs	Antiproliferative activity against human U266 cells after 72 hrs by MTT assay, IC50 = 7.57 μM.	24280069
A549	Antiproliferative assay		72 hrs	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay, IC50 = 17.3 μM.	24280069
MCF7	Cytotoxicity assay		24 hrs	Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay, IC50 = 32.2 μM.	26174555
SKOV3	Cytotoxicity assay		24 hrs	Cytotoxicity against human SKOV3 cells after 24 hrs by MTT assay, IC50 = 43.63 μM.	26174555
CT26	Cytotoxicity assay		72 hrs	Cytotoxicity against mouse CT26 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTS assay, IC50 = 7.7 μM.	26318057
SW620	Cytotoxicity assay		72 hrs	Cytotoxicity against human SW620 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTS assay, IC50 = 12.52 μM.	26318057
SGC7901	Cytotoxicity assay		72 hrs	Cytotoxicity against human SGC7901 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTS assay, IC50 = 24.41 μM.	26318057
GES-1	Cytotoxicity assay		72 hrs	Cytotoxicity against human GES-1 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTS assay, IC50 = 27.89 μM.	26318057
MGC803	Cytotoxicity assay		72 hrs	Cytotoxicity against human MGC803 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTS assay, IC50 = 31.39 μM.	26318057
U2OS	Cytotoxicity assay		48 hrs	Cytotoxicity against human U2OS cells expressing wild-type p53 after 48 hrs by crystal violet-staining based spectrophotometric assay, EC50 = 22.8 μM.	26606246
Saos2	Cytotoxicity assay		48 hrs	Cytotoxicity against human Saos2 cells expressing p53 mutant after 48 hrs by crystal violet-staining based spectrophotometric assay, EC50 = 28.1 μM.	26606246
U2OS	Cytotoxicity assay		24 hrs	Cytotoxicity against human U2OS cells expressing wild-type p53 after 24 hrs by crystal violet-staining based spectrophotometric assay, EC50 = 34.4 μM.	26606246
Saos2	Cytotoxicity assay		24 hrs	Cytotoxicity against human Saos2 cells expressing p53 mutant after 24 hrs by crystal violet-staining based spectrophotometric assay, EC50 = 48.6 μM.	26606246
T98G	Antiproliferative assay		24 hrs	Antiproliferative activity against human T98G cells after 24 hrs by EZ-Tox assay, IC50 = 10 μM.	26631318
T98G	Antiproliferative assay		72 hrs	Antiproliferative activity against human T98G cells assessed as cell viability after 72 hrs by MTT assay, IC50 = 25 μM.	26631318
SH-SY5Y	Antiproliferative assay		48 hrs	Antiproliferative activity against human SH-SY5Y cells assessed as cell viability after 48 hrs, IC50 = 16.1 μM.	26705144
HeLa	Antiproliferative assay		48 hrs	Antiproliferative activity against human HeLa cells assessed as cell viability after 48 hrs, IC50 = 21.8 μM.	26705144
HeLa	Cell cycle assay		16 hrs	Cell cycle arrest in human HeLa cells assessed as accumulation at G1 phase at IC50 to 2 times IC50 after 16 hrs by FACS analysis	26705144
HuH7	Cytotoxicity assay		48 hrs	Cytotoxicity against human HuH7 cells after 48 hrs by MTT assay, IC50 = 48 μM.	26783179
HeLa	Antiproliferative assay		3 days	Antiproliferative activity against human HeLa cells assessed as inhibition of cell viability after 3 days by WST-1 assay, IC50 = 12.11 μM.	26827161
LNCAP	Antiproliferative assay		3 days	Antiproliferative activity against human LNCAP cells assessed as inhibition of cell viability after 3 days by WST-1 assay, IC50 = 13.61 μM.	26827161
PC3	Antiproliferative assay		3 days	Antiproliferative activity against human PC3 cells assessed as inhibition of cell viability after 3 days by WST-1 assay, IC50 = 25.43 μM.	26827161
DU145	Antiproliferative assay		3 days	Antiproliferative activity against human DU145 cells assessed as inhibition of cell viability after 3 days by WST-1 assay, IC50 = 26.23 μM.	26827161
RAW264.7	Antiinflammatory assay		24 hrs	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay, IC50 = 19.1 μM.	26841168
BV2	Function assay		24 hrs	Inhibition of LPS-induced NO production in mouse BV2 cells assessed as reduction in nitrite level pretreated for 24 hrs before LPS treatment for additional 24 hrs by Griess reaction method, IC50 = 0.52 μM.	26859776
A549/CDDP	Antiproliferative assay		72 hrs	Antiproliferative activity against human A549/CDDP cells after 72 hrs by MTT assay, IC50 = 32.39 μM.	26891099
A549	Antiproliferative assay		72 hrs	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay, IC50 = 38.12 μM.	26891099
bone marrow	Function assay		48 hrs	Inhibition of RANKL-induced osteoclast differentiation in ICR mouse bone marrow cells after 48 hrs by TRAP assay, IC50 = 7.5 μM.	26923696
U937	Function assay		17 to 20 hrs	Inhibition of LPS/IFN-gamma-induced PGE2 production in PMA-treated human U937 cells after 17 to 20 hrs by enzyme immunoassay, IC50 = 1.88 μM.	27040659
RAW264.7	Function assay		17 to 20 hrs	Inhibition of LPS/IFN-gamma-induced PGE2 production in mouse RAW264.7 cells after 17 to 20 hrs by enzyme immunoassay, IC50 = 15.95 μM.	27040659
BV2	Antiinflammatory assay		24 hrs	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay, IC50 = 4.77 μM.	27228227
SH-SY5Y	Neuroprotective assay		24 hrs	Neuroprotective activity in human SH-SY5Y cells assessed as reduction in 6-OHDA-induced cell death measured after 24 hrs by MTT assay, EC50 = 6.2 μM.	27420919
PC3	Cytotoxicity assay		24 hrs	Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 24 hrs by MTT assay, IC50 = 39 μM.	27496212
DU145	Cytotoxicity assay		24 hrs	Cytotoxicity against human DU145 cells assessed as decrease in cell viability after 24 hrs by MTT assay, IC50 = 41 μM.	27496212
4T1	Cytotoxicity assay		24 hrs	Cytotoxicity against mouse 4T1 cells assessed as decrease in cell viability after 24 hrs by MTT assay, IC50 = 49.4 μM.	27496212
T47D	Cytotoxicity assay		24 hrs	Cytotoxicity against human T47D cells assessed as reduction in cell viability after 24 hrs by MTT assay, IC50 = 30 μM.	27517806
CHOK1	Growth inhibition assay		72 hrs	Growth inhibition of CHOK1 cells assessed as mitochondrial activity measured after 72 hrs by MTT assay, IC50 = 7.5 μM.	27521589
LNCAP	Antiproliferative assay		3 days	Antiproliferative activity against human LNCAP cells after 3 days by WST or trypan blue assay, IC50 = 13.61 μM.	27543391
PC3	Antiproliferative assay		3 days	Antiproliferative activity against human PC3 cells after 3 days by WST or trypan blue assay, IC50 = 25.43 μM.	27543391
DU145	Antiproliferative assay		3 days	Antiproliferative activity against human DU145 cells after 3 days by WST or trypan blue assay, IC50 = 26.23 μM.	27543391
BV2	Function assay		1 hr	Inhibition of LPS-induced nitric oxide production in mouse BV2 cells pretreated for 1 hr before LPS stimulation measured after 24 hrs by Griess assay, IC50 = 1.42 μM.	27592135
BV2	Function assay		1 hr	Inhibition of Src in mouse BV2 cells assessed as suppression of LPS-induced NO release preincubated for 1 hr followed by LPS addition measured after 24 hrs by Griess assay, IC50 = 2.2 μM.	27617803
A549	Antiproliferative assay		48 hrs	Antiproliferative activity against human A549 cells after 48 hrs by MTT assay, IC50 = 15.06 μM.	27816267
MDA-MB-231	Antiproliferative assay		48 hrs	Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay, IC50 = 24.54 μM.	27816267
DU145	Antiproliferative assay		48 hrs	Antiproliferative activity against human DU145 cells after 48 hrs by MTT assay, IC50 = 44.71 μM.	27816267
EAhy926	Cytotoxicity assay		48 hrs	Cytotoxicity against human EAhy926 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 24.6 μM.	27843113
Caco2	Cytotoxicity assay		48 hrs	Cytotoxicity against human Caco2 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 38.3 μM.	27843113
CHOK1	Cytotoxicity assay		48 hrs	Cytotoxicity against CHOK1 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 42 μM.	27843113
NCI-H1650	Cytotoxicity assay		72 hrs	Cytotoxicity against human NCI-H1650 cells measured after 72 hrs by MTT assay, IC50 = 20.7 μM.	27886548
LLC	Cytotoxicity assay		72 hrs	Cytotoxicity against mouse LLC cells measured after 72 hrs by MTT assay, IC50 = 24.7 μM.	27886548
A549	Cytotoxicity assay		72 hrs	Cytotoxicity against human A549 cells measured after 72 hrs by MTT assay, IC50 = 27.8 μM.	27886548
K562	Function assay		48 hrs	Inhibition of P-gp in doxorubicin resistant human K562 cells assessed as reduction in cell viability measured after 48 hrs by Presto blue assay, GI50 = 19.67 μM.	27908756
K562	Antiproliferative assay		48 hrs	Antiproliferative activity against human K562 cells measured after 48 hrs by Presto blue assay, GI50 = 21.67 μM.	27908756
H460	Function assay		48 hrs	Inhibition of P-gp in drug resistant human H460 cells assessed as reduction in cell viability measured after 48 hrs by sulforhodamine B assay, GI50 = 26 μM.	27908756
NCI-H460	Antiproliferative assay		48 hrs	Antiproliferative activity against human NCI-H460 cells measured after 48 hrs by sulforhodamine B assay, GI50 = 27.3 μM.	27908756
Raji	Function assay		48 hrs	Inhibition of TPA-induced EBV-early antigen activation in human Raji cells after 48 hrs by indirect immunofluorescence method, IC50 = 12.1 μM.	27933896
BV2	Function assay		24 hrs	Inhibition of LPS-induced nitric oxide production in mouse BV2 cells pretreated for 24 hrs followed by LPS-stimulation after 24 hrs by Griess assay, IC50 = 2.36 μM.	28032759
PC3	Antiproliferative assay		48 hrs	Antiproliferative activity against human PC3 cells after 48 hrs by MTT assay, IC50 = 12.22 μM.	28038323
MDA-MB-231	Antiproliferative assay		48 hrs	Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay, IC50 = 15.81 μM.	28038323
BT549	Antiproliferative assay		48 hrs	Antiproliferative activity against human BT549 cells after 48 hrs by MTT assay, IC50 = 24.93 μM.	28038323
HeLa	Antiproliferative assay		48 hrs	Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay, IC50 = 25.41 μM.	28038323
DU145	Antiproliferative assay		48 hrs	Antiproliferative activity against human DU145 cells after 48 hrs by MTT assay, IC50 = 28.41 μM.	28038323
BV2	Function assay		30 mins	Inhibition of LPS-induced nitric oxide production in mouse BV2 cells pretreated for 30 mins followed by LPS-stimulation after 24 hrs by Griess assay, IC50 = 4.5 μM.	28055210
THP1	Function assay		1 hr	Inhibition of LPS-induced tissue factor procoagulant activity in human THP1 cells preincubated for 1 hr followed by LPS addition measured after 5 hrs using factor 10a chromogenic substrate in presence of prothrombin complex, IC50 = 0.01316 μM.	28109788
BT474	Antiproliferative assay		24 hrs	Antiproliferative activity against HER2 positive human BT474 cells after 24 hrs by MTT assay, IC50 = 30.14 μM.	28319780
MDA-MB-453	Antiproliferative assay		24 hrs	Antiproliferative activity against HER2 positive human MDA-MB-453 cells after 24 hrs by MTT assay, IC50 = 32.4 μM.	28319780
MDA-MB-157	Antiproliferative assay		24 hrs	Antiproliferative activity against human MDA-MB-157 cells after 24 hrs by MTT assay, IC50 = 40.38 μM.	28319780
SKBR3	Antiproliferative assay		24 hrs	Antiproliferative activity against HER2 positive human SKBR3 cells after 24 hrs by MTT assay, IC50 = 42.83 μM.	28319780
MCF7	Antiproliferative assay		24 hrs	Antiproliferative activity against ER/PR positive human MCF7 cells after 24 hrs by MTT assay, IC50 = 42.89 μM.	28319780
MDA-MB-468	Antiproliferative assay		24 hrs	Antiproliferative activity against human MDA-MB-468 cells after 24 hrs by MTT assay, IC50 = 42.89 μM.	28319780
T47D	Antiproliferative assay		24 hrs	Antiproliferative activity against ER/PR positive human T47D cells after 24 hrs by MTT assay, IC50 = 47.91 μM.	28319780
PWR-1E	Antiproliferative assay		3 days	Antiproliferative activity against human PWR-1E cells after 3 days by WST-1 assay, IC50 = 8.14 μM.	28388523
LNCAP	Antiproliferative assay		3 days	Antiproliferative activity against human LNCAP cells after 3 days by WST-1 assay, IC50 = 13.61 μM.	28388523
PC3	Antiproliferative assay		3 days	Antiproliferative activity against human PC3 cells after 3 days by WST-1 assay, IC50 = 25.43 μM.	28388523
DU145	Antiproliferative assay		3 days	Antiproliferative activity against human DU145 cells after 3 days by WST-1 assay, IC50 = 26.23 μM.	28388523
BV2	Antineuroinflammatory assay		15 mins	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production pretreated for 15 mins followed by LPS-stimulation measured after 24 hrs by colorimetric method, IC50 = 6 μM.	28514148
L428	Function assay		2 hrs	Inhibition of NF-kappaB (unknown origin) expressed in human L428 cells after 2 hrs by luciferase reporter gene assay	28526369
PWR-1E	Antiproliferative assay		3 days	Antiproliferative activity against human PWR-1E cells expressing androgen receptor and PSA assessed as decrease in cell viability after 3 days by WST assay, IC50 = 8.85 μM.	28601720
LNCAP	Antiproliferative assay		3 days	Antiproliferative activity against human LNCAP cells assessed as decrease in cell viability after 3 days by WST assay, IC50 = 13.61 μM.	28601720
PC3	Antiproliferative assay		3 days	Antiproliferative activity against human PC3 cells assessed as decrease in cell viability after 3 days by WST assay, IC50 = 25.43 μM.	28601720
DU145	Antiproliferative assay		3 days	Antiproliferative activity against human DU145 cells assessed as decrease in cell viability after 3 days by WST assay, IC50 = 26.23 μM.	28601720
MDA-MB-231	Cytotoxicity assay		48 hrs	Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 12 μM.	28654265
HT-29	Cytotoxicity assay		48 hrs	Cytotoxicity against human HT-29 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 14 μM.	28654265
NCI-H460	Cytotoxicity assay		48 hrs	Cytotoxicity against human NCI-H460 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 15 μM.	28654265
LN229	Cytotoxicity assay		48 hrs	Cytotoxicity against human LN229 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 15 μM.	28654265
U87	Cytotoxicity assay		48 hrs	Cytotoxicity against human U87 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 16 μM.	28654265
COLO201	Cytotoxicity assay		48 hrs	Cytotoxicity against human COLO201 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 18 μM.	28654265
A549	Cytotoxicity assay		48 hrs	Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 18 μM.	28654265
T47D	Cytotoxicity assay		48 hrs	Cytotoxicity against human T47D cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 19 μM.	28654265
MML1	Cytotoxicity assay		48 hrs	Cytotoxicity against human MML1 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 19 μM.	28654265
BxPC3	Cytotoxicity assay		48 hrs	Cytotoxicity against human BxPC3 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 29 μM.	28654265
SK-MEL-2	Cytotoxicity assay		48 hrs	Cytotoxicity against human SK-MEL-2 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 36 μM.	28654265
HCT116	Antiproliferative assay		48 hrs	Antiproliferative activity against human HCT116 cells after 48 hrs by sulforhodamine B assay, GI50 = 3.5 μM.	28716495
HCT116	Function assay		48 hrs	Induction of mitochondrial membrane potential loss in human HCT116 cells at antiproliferative GI50 after 48 hrs by JC-1 staining based fluorescence assay	28716495
HCT116	Function assay		60 mins	Induction of mitochondrial superoxide generation in human HCT116 cells at antiproliferative GI50 after 60 mins by mitoSOX red-probe based fluorescence assay	28716495
HCT116	Function assay		60 mins	Induction of reactive oxygen species generation in human HCT116 cells at antiproliferative GI50 after 60 mins by DCFH-DA probe based assay	28716495
MCF7	Function assay		48 hrs	Modulation activity at ER in human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay, IC50 = 48 μM.	28797797
BV2	Antiinflammatory assay		24 hrs	Antiinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by griess assay, IC50 = 4 μM.	29035525
NCI-H460	Cytotoxicity assay		48 hrs	Cytotoxicity against human NCI-H460 cells assessed as inhibition of cell viability after 48 hrs by MTT assay, IC50 = 17.14 μM.	29174816
PC3	Cytotoxicity assay		48 hrs	Cytotoxicity against human PC3 cells assessed as inhibition of cell viability after 48 hrs by MTT assay, IC50 = 18.71 μM.	29174816
DU145	Cytotoxicity assay		48 hrs	Cytotoxicity against human DU145 cells assessed as inhibition of cell viability after 48 hrs by MTT assay, IC50 = 33.12 μM.	29174816
HepG2	Function assay		5 hrs	Activation of Nrf2 (unknown origin) expressed in human HepG2 cells after 5 hrs by ARE-driven luciferase reporter gene assay, EC50 = 21 μM.	29223100
BGC823	Cytotoxicity assay		72 hrs	Cytotoxicity against human BGC823 cells assessed as cell growth inhibition after 72 hrs by MTT assay, IC50 = 19.5 μM.	29288946
NCI-H460	Cytotoxicity assay		72 hrs	Cytotoxicity against human NCI-H460 cells assessed as cell growth inhibition after 72 hrs by MTT assay, IC50 = 20.3 μM.	29288946
SGC7901	Cytotoxicity assay		72 hrs	Cytotoxicity against human SGC7901 cells assessed as cell growth inhibition after 72 hrs by MTT assay, IC50 = 26.2 μM.	29288946
HepG2	Cytotoxicity assay		24 hrs	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 24 hrs by MTT assay, IC50 = 13.78 μM.	29421568
THP1	Cytotoxicity assay		24 hrs	Cytotoxicity against human THP1 cells assessed as reduction in cell viability after 24 hrs by MTT assay, IC50 = 22.54 μM.	29421568
K562	Cytotoxicity assay		24 hrs	Cytotoxicity against human K562 cells assessed as reduction in cell viability after 24 hrs by MTT assay, IC50 = 23.12 μM.	29421568
LO2	Cytotoxicity assay		24 hrs	Cytotoxicity against human LO2 cells assessed as reduction in cell viability after 24 hrs by MTT assay, IC50 = 25.67 μM.	29421568
HeLa	Cytotoxicity assay		24 hrs	Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 24 hrs by MTT assay, IC50 = 31.89 μM.	29421568
NCI-H460	Cytotoxicity assay		48 hrs	Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability after 48 hrs by MTT assay, IC50 = 17.11 μM.	29429834
PC3	Cytotoxicity assay		48 hrs	Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 48 hrs by MTT assay, IC50 = 18.65 μM.	29429834
DU145	Cytotoxicity assay		48 hrs	Cytotoxicity against human DU145 cells assessed as reduction in cell viability after 48 hrs by MTT assay, IC50 = 33.15 μM.	29429834
MCF7	Cytotoxicity assay		4 hrs	Cytotoxicity against human MCF7 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay, IC50 = 22.1 μM.	29605808
WRL68	Cytotoxicity assay		4 hrs	Cytotoxicity against human WRL68 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay, IC50 = 27.8 μM.	29605808
A549	Cytotoxicity assay		4 hrs	Cytotoxicity against human A549 cells preincubated for 4 hrs followed by incubation in compound free media for 24 hrs by MTT assay, IC50 = 43.9 μM.	29605808
NCI-H1975	Cytotoxicity assay		72 hrs	Cytotoxicity against human NCI-H1975 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50 = 1.4 μM.	29655083
A549	Cytotoxicity assay		72 hrs	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50 = 6.8 μM.	29655083
HL7702	Cytotoxicity assay		72 hrs	Cytotoxicity against human HL7702 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50 = 7.2 μM.	29655083
H460	Cytotoxicity assay		72 hrs	Cytotoxicity against human H460 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50 = 8.5 μM.	29655083
NCI-H1650	Cytotoxicity assay		72 hrs	Cytotoxicity against human NCI-H1650 cells assessed as reduction in cell viability after 72 hrs by MTT assay, IC50 = 20.5 μM.	29655083
HepG2	Cytotoxicity assay		24 hrs	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 24 hrs by MTT assay, IC50 = 10 μM.	29798827
HCT116	Antiproliferative assay		72 hrs	Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay, IC50 = 10.91 μM.	29886021
HepG2	Cytotoxicity assay		24 hrs	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 24 hrs by MTT assay, IC50 = 16.01 μM.	29909338
SMMC7721	Cytotoxicity assay		24 hrs	Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability after 24 hrs by MTT assay, IC50 = 19.47 μM.	29909338
QGY7703	Cytotoxicity assay		24 hrs	Cytotoxicity against human QGY7703 cells assessed as reduction in cell viability after 24 hrs by MTT assay, IC50 = 21.44 μM.	29909338
HHL5	Cytotoxicity assay		24 hrs	Cytotoxicity against human HHL5 cells assessed as reduction in cell viability after 24 hrs by MTT assay, IC50 = 22.27 μM.	29909338
LO2	Cytotoxicity assay		24 hrs	Cytotoxicity against human LO2 cells assessed as reduction in cell viability after 24 hrs by MTT assay, IC50 = 23.87 μM.	29909338
HT22	Cytotoxicity assay		24 hrs	Cytotoxicity against mouse HT22 cells assessed as reduction in cell viability after 24 hrs by MTT assay, MNTD = 1 μM.	30031653
LNCAP	Antiproliferative assay		3 days	Antiproliferative activity against androgen-sensitive human LNCAP cells assessed as decrease in cell viability after 3 days by WST assay, IC50 = 13.61 μM.	30121214
PC3	Antiproliferative assay		3 days	Antiproliferative activity against androgen-insensitive human PC3 cells assessed as decrease in cell viability after 3 days by WST assay, IC50 = 25.43 μM.	30121214
DU145	Antiproliferative assay		3 days	Antiproliferative activity against androgen-insensitive human DU145 cells assessed as decrease in cell viability after 3 days by WST assay, IC50 = 26.23 μM.	30121214
MCF7	Cytotoxicity assay		72 hrs	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay, IC50 = 9.8 μM.	30138803
EJ28	Cytotoxicity assay		72 hrs	Cytotoxicity against human EJ28 cells after 72 hrs by MTT assay, IC50 = 10.5 μM.	30138803
HT1080	Cytotoxicity assay		72 hrs	Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay, IC50 = 13.1 μM.	30138803
SW620	Cytotoxicity assay		72 hrs	Cytotoxicity against human SW620 cells after 72 hrs by MTT assay, IC50 = 14 μM.	30138803
A549	Cytotoxicity assay		72 hrs	Cytotoxicity against human A549 cells after 72 hrs by MTT assay, IC50 = 17.9 μM.	30138803
SMMC7721	Cytotoxicity assay		24 hrs	Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay, IC50 = 16.68 μM.	30771605
HepG2	Cytotoxicity assay		24 hrs	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay, IC50 = 17.37 μM.	30771605
LO2	Cytotoxicity assay		24 hrs	Cytotoxicity against human LO2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay, IC50 = 22.69 μM.	30771605
QGY7703	Cytotoxicity assay		24 hrs	Cytotoxicity against human QGY7703 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay, IC50 = 23.91 μM.	30771605
HHL5	Cytotoxicity assay		24 hrs	Cytotoxicity against human HHL5 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay, IC50 = 24.19 μM.	30771605
HCT-116	Function assay		24 hrs	Inhibition of chymotrypsin-like activity of human 26S proteasome in human HCT-116 cells assessed as decrease in AMC hydrolysis using Suc-LLVY-AMC as substrate preincubated for 24 hrs followed by addition of substrate and measured after 2 hrs by fluorometr, IC50 = 10 μM.	30776692
SW480	Function assay		24 hrs	Inhibition of chymotrypsin-like activity of human 26S proteasome in human SW480 cells assessed as decrease in AMC hydrolysis using Suc-LLVY-AMC as substrate preincubated for 24 hrs followed by addition of substrate and measured after 2 hrs by fluorometric, IC50 = 10 μM.	30776692
RAW264.7	Function assay		0.5 hrs	Inhibition of MAPK/NFkappaB in ICR mouse RAW264.7 cells assessed as reduction in LPS-induced TNFalpha production pretreated for 0.5 hrs followed by LPS challenge and measured after 24 hrs by ELISA	30780088
RAW264.7	Function assay		0.5 hrs	Inhibition of LPS-induced NFkappaB p65 nuclear translocation in ICR mouse RAW264.7 cells preincubated for 0.5 hrs followed by LPS challenge and measured after 40 mins by DAPI staining-based immunofluorescence microscopic analysis	30780088
RAW264.7	Function assay		0.5 hrs	Inhibition of MAPK/NFkappaB in ICR mouse RAW264.7 cells assessed as reduction in LPS-induced IL6 production pretreated for 0.5 hrs followed by LPS challenge and measured after 24 hrs by ELISA	30780088
RAW264.7	Function assay		4 hrs	Inhibition of RANKL-induced osteoclastogenesis in murine RAW264.7 cells assessed as formation of TRAP positive multinucleated osteoclasts pretreated for 4 hrs followed by RANKL addition measured after 72 hrs, IC50 = 3 μM.	30794412
BV2	Antineuroinflammatory assay		1 hr	Anti-neuroinflammatory activity in human BV2 cells assessed as inhibition of LPS-induced NO production preincubated for 1 hr followed by LPS stimulation measured after 24 hrs by Griess reagent based assay, IC50 = 1.58 μM.	31005056
SKOV3	Cytotoxicity assay		48 hrs	Cytotoxicity against human SKOV3 Cells after 48 hrs by Cell Titer Blue assay, IC50 = 6.7 μM.	31129455
U87MG	Cytotoxicity assay		48 hrs	Cytotoxicity against human U87MG Cells 48 hrs by MTS assay, IC50 = 7.15 μM.	31129455
LX2	Cytotoxicity assay		48 hrs	Cytotoxicity against human LX2 Cells after 48 hrs by MTT assay, IC50 = 9.14 μM.	31129455
MCF7	Cytotoxicity assay		48 hrs	Cytotoxicity against human MCF7 Cells after 48 hrs by MTT assay, IC50 = 9.28 μM.	31129455
HepG2	Cytotoxicity assay		48 hrs	Cytotoxicity against human HepG2 Cells after 48 hrs by MTT assay, IC50 = 9.44 μM.	31129455
3T3	Cytotoxicity assay		48 hrs	Cytotoxicity against mouse 3T3 Cells after 48 hrs by MTT assay, IC50 = 9.51 μM.	31129455
MDA-MB-231	Anticancer assay		72 hrs	Anticancer activity against human MDA-MB-231 cells after 72 hrs by MTT assay, IC50 = 11.45 μM.	31129455
SMMC7721	Anticancer assay		72 hrs	Anticancer activity against human SMMC7721 cells after 72 hrs by MTT assay, IC50 = 12.57 μM.	31129455
LX2	Anticancer assay		72 hrs	Anticancer activity against human LX2 cells after 72 hrs by MTT assay, IC50 = 13.97 μM.	31129455
SW480	Cytotoxicity assay		24 to 48 hrs	Cytotoxicity against human SW480 Cells after 24 to 48 hrs by MTT assay, IC50 = 15 μM.	31129455
U251MG	Cytotoxicity assay		48 hrs	Cytotoxicity against human U251MG cells after 48 hrs by MTS assay, IC50 = 15.26 μM.	31129455
EAhy926	Cytotoxicity assay		72 hrs	Cytotoxicity against human EAhy926 Cells after 72 hrs by MTT assay, IC50 = 20 μM.	31129455
HepG2	Cytotoxicity assay		72 hrs	Cytotoxicity against human HepG2 Cells after 72 hrs by MTT assay, IC50 = 20.3 μM.	31129455
CHOK1	Cytotoxicity assay		72 hrs	Cytotoxicity against human CHOK1 Cells after 72 hrs by MTT assay, IC50 = 21.3 μM.	31129455
HT-29	Cytotoxicity assay		72 hrs	Cytotoxicity against human HT-29 Cells after 72 hrs by MTT assay, IC50 = 23.4 μM.	31129455
A549	Cytotoxicity assay		24 to 48 hrs	Cytotoxicity against human A549 Cells after 24 to 48 hrs by MTT assay, IC50 = 28 μM.	31129455
Caco2	Cytotoxicity assay		72 hrs	Cytotoxicity against human Caco2 Cells after 72 hrs by MTT assay, IC50 = 28 μM.	31129455
MDCK2	Cytotoxicity assay		15 mins	Cytotoxicity against mitoxantrone resistant human MDCK2 cells expressing BCRP after 15 mins by fluorescence assay, IC50 = 32 μM.	31129455
K562	Cytotoxicity assay		24 to 48 hrs	Cytotoxicity against human K562 Cells after 24 to 48 hrs by MTT assay, IC50 = 36 μM.	31129455
GBM1	Cytotoxicity assay		48 hrs	Cytotoxicity against human GBM1 Cells 48 hrs by MTS assay, IC50 = 39.65 μM.	31129455
PC12	Cytotoxicity assay		24 hrs	Cytotoxicity against rat PC12 cells measured after 24 hrs by WST8/PMS assay, CC50 = 42 μM.	31262559
HCT116	Antiproliferative assay		72 hrs	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay, IC50 = 10.5 μM.	31326241
KM12	Cytotoxicity assay		4 hrs	Cytotoxicity against human KM12 cells after 4 hrs by MTT assay, IC50 = 16 μM.	31336310
SW480	Cytotoxicity assay		4 hrs	Cytotoxicity against human SW480 cells after 4 hrs by MTT assay, IC50 = 17.94 μM.	31336310
HT-29	Cytotoxicity assay		4 hrs	Cytotoxicity against human HT-29 cells after 4 hrs by MTT assay, IC50 = 20.73 μM.	31336310
WiDr	Cytotoxicity assay		4 hrs	Cytotoxicity against human WiDr cells after 4 hrs by MTT assay, IC50 = 26.82 μM.	31336310
SW116	Cytotoxicity assay		4 hrs	Cytotoxicity against human SW116 cells after 4 hrs by MTT assay, IC50 = 27.46 μM.	31336310
Colon 26	Cytotoxicity assay		4 hrs	Cytotoxicity against mouse Colon 26 cells after 4 hrs by MTT assay, IC50 = 30.34 μM.	31336310
PC3	Antiproliferative assay		48 hrs	Antiproliferative activity human PC3 cells assessed as reduction in Ki67 protein level at GI50 incubated for 48 hrs by DAPI staining based immunofluorescence assay	31767266
22Rv1	Antiproliferative assay		48 hrs	Antiproliferative activity human 22Rv1 cells assessed as reduction in Ki67 protein level at GI50 incubated for 48 hrs by DAPI staining based immunofluorescence assay	31767266
Sf21	Function assay		10 mins	Inhibition of recombinant human full-length His6-tagged p300 expressed in baculovirus infected Sf21 insect cells using [3H]acetylCoA as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by liquid scintillation co, IC50 = 25 μM.	31910017
COS7	Function assay		10 mins	Inhibition of rabbit SERCA1b expressed in COS7 cells microsomal membranes preincubated for 10 mins followed by addition of ATP and measured after 40 mins by ELISA method, Ki = 5.8 μM.	32030976
COS7	Function assay		10 mins	Inhibition of human SERCA3a expressed in COS7 cells microsomal membranes preincubated for 10 mins followed by addition of ATP and measured after 40 mins by ELISA method, Ki = 8.6 μM.	32030976
A549	Function assay		15 mins	Inhibition of microsomal PGES1 in ILbeta-stimulated human A549 cells using PGH2 as substrate preincubated for 15 mins followed by substrate addition measured after 1 min by RP-HPLC analysis, IC50 = 0.2 μM.	ChEMBL
RAW264.7	Function assay		20 hrs	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells measured after 20 hrs by griess assay, IC50 = 0.8 μM.	ChEMBL
MDCK	Antiviral assay		40 hrs	Antiviral activity against Influenza A virus (A/WSN/33(H1N1)) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect incubated for 40 hrs by CellTiter-Glo assay, IC50 = 6.7 μM.	ChEMBL
MDCK	Antiviral assay		40 hrs	Antiviral activity against Influenza A virus (A/WSN/33(H1N1)) infected in MDCK cells assessed as reduction in virus-induced cytopathic effect after 40 hrs by CellTiter-Glo assay, IC50 = 6.7 μM.	ChEMBL
RAW264.7	Antiinflammatory assay		17 hrs	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS/IFN-gamma-stimulated nitric oxide production after 17 hrs by Griess assay, IC50 = 14.7 μM.	ChEMBL
MDCK	Cytotoxicity assay		40 hrs	Cytotoxicity against MDCK cells assessed as decrease in cell viability after 40 hrs by CellTiter-Glo assay, CC50 = 48.3 μM.	ChEMBL
MDCK	Cytotoxicity assay		40 hrs	Cytotoxicity against MDCK cells assessed as reduction in cell viability after 40 hrs by CellTiter-Glo assay, CC50 = 48.3 μM.	ChEMBL
Caco2	Antiproliferative assay			Antiproliferative activity against human Caco2 cells, IC50 = 10.3 μM.	26539626
T67	Neuroprotective assay			Neuroprotective effect in human T67 cells assessed as increase in NQO1 activity using DCIP as substrate	26696252
HepG2	Cytotoxicity assay			Cytotoxicity activity against human HepG2 cells by MTT assay, IC50 = 4.43 μM.	26873414
A549	Cytotoxicity assay			Cytotoxicity against human A549 cells by MTT assay, IC50 = 23.54 μM.	26873414
MCF7	Cytotoxicity assay			Cytotoxicity against human MCF7 cells by MTT assay, IC50 = 46.25 μM.	26873414
SKOV3	Cytotoxicity assay			Cytotoxicity against human SKOV3 cells by MTT assay, IC50 = 49.85 μM.	26873414
A549/CDDP	Function assay			Inhibition of thioredoxin reductase in human A549/CDDP cells by DTNB dye based microplate spectrophotometry, IC50 = 39.42 μM.	26891099
A549	Function assay			Inhibition of thioredoxin reductase in human A549 cells by DTNB dye based microplate spectrophotometry, IC50 = 40.56 μM.	26891099
HCT116	Antiproliferative assay			Antiproliferative activity against human HCT116 cells up to 72 hrs by MTT assay, IC50 = 13.77 μM.	28319780
MDA-MB-231	Antiproliferative assay			Antiproliferative activity against human MDA-MB-231 cells up to 72 hrs by MTT assay, IC50 = 16.23 μM.	28319780
PC3	Antiproliferative assay			Antiproliferative activity against human PC3 cells up to 72 hrs by MTT assay, IC50 = 18.8 μM.	28319780
A549	Growth inhibition assay			Growth inhibition of human A549 cells, IC50 = 23 μM.	28814374
Vero	Cytotoxicity assay			Cytotoxicity against African green monkey Vero cells assessed as growth inhibition, CC50 = 36.8 μM.	29920454
LNCAP	Antiproliferative assay			Antiproliferative activity against androgen-sensitive human LNCAP cells, IC50 = 2 μM.	30121214
DU145	Antiproliferative assay			Antiproliferative activity against androgen-insensitive human DU145 cells, IC50 = 2 μM.	30121214
PC3	Antiproliferative assay			Antiproliferative activity against androgen-insensitive human PC3 cells, IC50 = 2 μM.	30121214
HeLa	Anticancer assay			Anticancer activity against human HeLa cells, IC50 = 4.33 μM.	31129455
HL60	Cytotoxicity assay			Cytotoxicity against human HL60 cells, IC50 = 6.3 μM.	31129455
HSC4	Cytotoxicity assay			Cytotoxicity against human HSC4 cells, IC50 = 7.1 μM.	31129455
HSC2	Cytotoxicity assay			Cytotoxicity against human HSC2 cells, IC50 = 7.8 μM.	31129455
AGS	Cytotoxicity assay			Cytotoxicity against human AGS Cells, IC50 = 9.77 μM.	31129455
HSC3	Cytotoxicity assay			Cytotoxicity against human HSC3 cells, IC50 = 11 μM.	31129455
HeLa	Cytotoxicity assay			Cytotoxicity against human HeLa Cells, IC50 = 14.03 μM.	31129455
RWPE1	Cytotoxicity assay			Cytotoxicity against human RWPE1 cells, IC50 = 15.62 μM.	31129455
MCF7	Apoptosis assay			Induction of apoptosis in human MCF7 cells, IC50 = 17.1 μM.	31129455
BGC823	Cytotoxicity assay			Cytotoxicity against human BGC823 cells, IC50 = 17.5 μM.	31129455
BxPC3	Cytotoxicity assay			Cytotoxicity against human BxPC3 cells, IC50 = 18.25 μM.	31129455
HT-29	Cytotoxicity assay			Cytotoxicity against human HT-29 Cells, IC50 = 18.74 μM.	31129455
H1299	Cytotoxicity assay			Cytotoxicity against human H1299 cells, IC50 = 18.93 μM.	31129455
PC3	Cytotoxicity assay			Cytotoxicity against human PC3 cells, IC50 = 19.98 μM.	31129455
AGS	Cytotoxicity assay			Cytotoxicity against human AGS Cells by MTT assay, IC50 = 20.76 μM.	31129455
PC3	Anticancer assay			Anticancer activity against human PC3 cells, IC50 = 20.9 μM.	31129455
MGC803	Cytotoxicity assay			Cytotoxicity against human MGC803 cells, IC50 = 21.2 μM.	31129455
Hep2	Cytotoxicity assay			Cytotoxicity against human Hep2 Cells, IC50 = 21.37 μM.	31129455
MCF7	Cytotoxicity assay			Cytotoxicity against human MCF7 cells, IC50 = 22.5 μM.	31129455
SGC7901	Cytotoxicity assay			Cytotoxicity against human SGC7901 cells, IC50 = 22.5 μM.	31129455
A549	Cytotoxicity assay			Cytotoxicity against human A549 Cells by MTT assay, IC50 = 25.33 μM.	31129455
MDA-MB-231	Cytotoxicity assay			Cytotoxicity against human MDA-MB-231 cells, IC50 = 26.5 μM.	31129455
GES-1	Cytotoxicity assay			Cytotoxicity against human GES-1 cells, IC50 = 29.1 μM.	31129455
DU145	Anticancer assay			Anticancer activity against human DU145 cells, IC50 = 31.7 μM.	31129455
HL-7702	Cytotoxicity assay			Cytotoxicity against human HL-7702 cells, IC50 = 32.33 μM.	31129455
HepG2	Cytotoxicity assay			Cytotoxicity against human HepG2 Cells, IC50 = 36.77 μM.	31129455
HeLa	Cytotoxicity assay			Cytotoxicity against human HeLa cells, IC50 = 8.2 μM.	31336310
B16	Cytotoxicity assay			Cytotoxicity against human B16 cells by MTT assay, IC50 = 8.5 μM.	31336310
HT1080	Function assay			Decrease in UPA expression in human HT1080 cells, IC50 = 10 μM.	31336310
Hep3B	Cytotoxicity assay			Cytotoxicity against human Hep3B cells, IC50 = 10 μM.	31336310
BT474	Cytotoxicity assay			Cytotoxicity against human BT474 cells, IC50 = 11 μM.	31336310
T47D	Cytotoxicity assay			Cytotoxicity against human T47D cells, IC50 = 11 μM.	31336310
HaCaT	Cytotoxicity assay			Cytotoxicity against human HaCaT cells, IC50 = 13 μM.	31336310
HPL1D	Cytotoxicity assay			Cytotoxicity against human HPL1D cells, IC50 = 16 μM.	31336310
MCF7	Cytotoxicity assay			Cytotoxicity against human MCF7 cells, IC50 = 19.9 μM.	31336310
22Rv1	Function assay			Inhibition of NFkappaB p65 nuclear translocation in human 22Rv1 cells at GI50 by DAPI staining based immunofluorescence assay	31767266
PC3	Function assay			Inhibition of NFkappaB p65 nuclear translocation in human PC3 cells at GI50 by DAPI staining based immunofluorescence assay	31767266
PC3	Function assay			Inhibition of NFkappaB p65 transcriptional activity in human PC3 cells at GI50 by sandwich ELISA	31767266
22Rv1	Function assay			Inhibition of NFkappaB p65 transcriptional activity in human 22Rv1 cells at GI50 by sandwich ELISA	31767266
HEK293T	Cytotoxicity assay			Cytotoxicity against HEK293T cells, IC50 = 15.2 μM.	ChEMBL
J774.1	Cytotoxicity assay			Cytotoxicity against mouse J774.1 cells, IC50 = 31 μM.	ChEMBL
他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

生物活性

製品説明

Targets

Nrf2 ^[1] (Cell-free assay)	Ferroptosis ^[9]	HDAC ^[7] (Cell-free assay)	NF-κB ^[8] (Cell-free assay)	p300 ^[6] (Cell-free assay)
				~25 μM

In Vitro
In vitro	Curcumin induces the expression of forkhead box protein O1 (FOXO1) through activation of extracellular signal-regulated kinase 1/2 signaling. Curcumin inhibits cell proliferation, which was associated with upregulation of the cyclin-dependent kinase inhibitors, p27 and p21, and downregulation of cyclin D1^[2]. Curcumin induces endoplasmic reticulum (ER) stress and mitochondrial dysfunction as evidenced by up-regulation of CCAAT/enhancer binding protein homologous protein (CHOP), phosphorylation of JNK and down-regulation of SERCA2ATPase, release of cytochrome c, decrease of Bcl-2 and reduction of mitochondrial membrane potential in both AGS and HT-29 cells^[3].
細胞実験	細胞株	murine melanoma cell subline(B16-R)
	濃度	0-100 μM
	反応時間	24-48 h
	実験の流れ	1×104 B16-R cells are cultivated as monolayer culture for 12 hr. They were then incubated in 200 μL of RPMI, 10% FBS containing curcumin at final concentrations from 1–100 μM in 96-multiwell plates for 24-48 hr. After these incubations, cells are washed twice in PBS and 500 μl of fresh culture medium containing MTT (0.3 mg/mL) are added for colorimetric assay.
実験結果図	Methods	Biomarkers	結果図	PMID
	Western blot	p-IRE1α / IREα HDAC1/ HDAC2 p-STAT3 / STAT3		29901626
	Immunofluorescence	β-catenin PKD1 EGFR / LC3		22523587
	Growth inhibition assay	Cell proliferation Cell viability		22523587

In Vivo
In Vivo	Chronic treatment with curcumin significantly reverses the CMS-induced behavioral abnormalities in stressed rats. Additionally, curcumin effectively inhibits cytokine gene expression at both the mRNA and the protein level and reduces the activation of NF-κB^[4].
動物実験	動物モデル	Female B6D2F1 mice (6-8 weeks old)
	投与量	25 mg/kg
	投与経路	i.p.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
臨床試験 (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT06053411	Not yet recruiting	Interaction	Washington State University	January 1 2024	Early Phase 1
NCT05947513	Not yet recruiting	Cervical Cancer	Addis Ababa University\|Center for Innovative Drug Development and Therapeutic Trials for Africa Addis Ababa University\|Akay Natural Ingredients Private Limited	October 2023	Phase 1\|Phase 2
NCT05966441	Not yet recruiting	Chemotherapy-induced Peripheral Neuropathy	Ain Shams University	August 30 2023	Phase 2
NCT05774704	Recruiting	Bioavailability\|Gut Microbiome\|Safety	Texas Tech University Health Sciences Center	August 21 2023	Phase 1\|Phase 2

参考
[1] Gao S, et al. Food Chem Toxicol. 2013, 59:739-47. [2] Nasiri M, et al. Asian Pac J Cancer Prev. 2013, 14(6):3449-3453. [3] Cao A, et al. Apoptosis. 2013, 18(11):1391-1402. [4] Jiang H, et al. Prog Neuropsychopharmacol Biol Psychiatry. 2013, 47:33-39. [5] Odot J, et al. Int J Cancer. 2004, 111(3):381-387. [6] Balasubramanyam K, et al. J Biol Chem. 2004, 279(49):51163-71. [7] Chen Y, et al. Basic Clin Pharmacol Toxicol. 2007, 101(6):427-33. [8] Singh S, et al. J Biol Chem. 1995, 270(42):24995-5000. [9] Melania Guerrero-Hue, et al. FASEB J . 2019 Aug;33(8):8961-8975. + 展開

参考

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化学情報

分子量	368.38	化学式	C₂₁H₂₀O₆
CAS No.	458-37-7	SDF	Download Curcumin SDFをダウンロードする
Smiles	COC1=C(C=CC(=C1)C=CC(=O)CC(=O)C=CC2=CC(=C(C=C2)O)OC)O
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1个月-20°Cin solvent

In vitro
Batch:

DMSO : 74 mg/mL ( (200.87 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : Insoluble

Ethanol : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):