Roscovitine (CYC202)

製品コード:S1153 別名:Seliciclib, R-roscovitine

For research use only.

Roscovitine (CYC202, Seliciclib, R-roscovitine) is a potent and selective CDK inhibitor for Cdc2, CDK2 and CDK5 with IC50 of 0.65 μM, 0.7 μM and 0.16 μM in cell-free assays. It shows little effect on CDK4/6. Phase 2.

Roscovitine (CYC202)化学構造

CAS No. 186692-46-6

サイズ 価格(税別)
10mM (1mL in DMSO) JPY 33700
JPY 26400
JPY 80000
JPY 163000

代表番号: 03-5615-9297|電子メール:[email protected]
よく尋ねられる質問

文献中Selleckの製品使用例(105)

製品安全説明書

CDK阻害剤の選択性比較

相関CDK製品

生物活性

製品説明 Roscovitine (CYC202, Seliciclib, R-roscovitine) is a potent and selective CDK inhibitor for Cdc2, CDK2 and CDK5 with IC50 of 0.65 μM, 0.7 μM and 0.16 μM in cell-free assays. It shows little effect on CDK4/6. Phase 2.
ターゲット
CDK5/p35 [1]
(Cell-free assay)
Cdc2/CyclinB [1]
(Cell-free assay)
CDK2/CyclinA [1]
(Cell-free assay)
CDK2/CyclinE [1]
(Cell-free assay)
ERK2 [1]
(Cell-free assay)
0.16 μM 0.65 μM 0.7 μM 0.7 μM 14 μM
体外試験

Roscovitine displays high efficiency and high selectivity towards some cyclin-dependent kinases with IC50 of 0.65, 0.7, 0.7 and 0.16 μM for cdc2/cyclin B, cdk2/cyclin A, cdk2/cyclin E and cdk5/p53, respectively. [1] Roscovitine reversibly inhibits the prophaselmetaphase transition in the micromolar range of starfish oocytes and sea urchin embryos, inhibits in vitro M-phase-promoting factor activity and in vitro DNA synthesis in Xenopus egg extracts, and suppresses the proliferation of mammalian cell lines with an average IC50 of 16 μM. [1] In mesangial cells, Roscovitine results in a dose-dependent reduction of CDK2 activity that at concentrations of 7.5, 12.5 and 25 mM, Roscovitine causes a 25, 50% and 100% decrease in CDK2 activity, respectively. [2] A recent study shows that Roscovitine inhibits cdk5 kinase activity, cell proliferation, multicellular development, and cdk5 nuclear translocation in Dictyostelium discoideum, without affecting the expression of cdk5 protein during axenic growth. [3]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LB771-HNC NUHETXhmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXvJR|UxRTR6LkmyNVIh|ryP NIfWWGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
LB2241-RCC NI\PRXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYnJR|UxRTR6Lk[yNFIh|ryP NEfEb5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
DU-4475 NVfROW5pT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVj6[m04UUN3ME20PE41QTN5IN88US=> Mn;GQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
LB2518-MEL MljPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTR5LkC0OFgh|ryP MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFc3Oi9pPmPBUmdGWjxxYU6=
NCI-H209 NXzT[2Q4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXzJR|UxRTR4LkCxNVUh|ryP NGfTS449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
CGTH-W-1 NFvWZZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHu3N5ZKSzVyPUS0Mlk3QTdizszN NFv6cIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
MS-1 NEHKPJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFq0R4xKSzVyPUSyMlg6OyEQvF2= NY\pVWFjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
GI-ME-N MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH6wcnJKSzVyPUSyMlY3PzFizszN MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFc3Oi9pPmPBUmdGWjxxYU6=
DG-75 M{HS[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTR{Lk[1OFYh|ryP NIfFXGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
MLMA MmDVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWTJR|UxRTR{LkK3PFch|ryP M3;GTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
HT NX;IfWVQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MW\JR|UxRTR{LkCwNlgh|ryP MkXTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
LC-1F NFTtZ2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M133NWlEPTB;NEGuOVcxPSEQvF2= NF3tTXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
NCI-H1882 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVvJR|UxRTRyLkW5PVgh|ryP M1rWb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
NTERA-S-cl-D1 M2HNVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2ewVGlEPTB;M{muOVg1OiEQvF2= NH7HVJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
NCI-H345 NHzOc3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXnJR|UxRTN6LkmxNFYh|ryP NFPMWJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
MONO-MAC-6 M2D3cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF7o[|NKSzVyPUO4MlI1PzdizszN NEPkZ5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
RS4-11 MoDFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnnLTWM2OD1|Nz63NFY6KM7:TR?= NXLWW5N5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
ML-2 NGr3eWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVTLSFVzUUN3ME2zO{43PzF{IN88US=> M2fJdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
OPM-2 NXzhV|c2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWrJV|I5UUN3ME2zO{4zQTR7IN88US=> NGTaNII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
LU-139 Mm\yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTN5LkG4OVYh|ryP M3HzSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
COLO-684 NVjSbolOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWjJR|UxRTN5LkCxNkDPxE1? M{HadlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
MOLT-4 M4Dsfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkPNTWM2OD1|Nj6zNlc3KM7:TR?= NFfkOXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
TE-6 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX\JR|UxRTN4LkOyOFYh|ryP MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFc3Oi9pPmPBUmdGWjxxYU6=
TE-441-T MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnnBTWM2OD1|Nj6xNVQ5KM7:TR?= M13NcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
IMR-5 NFLrNmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{nvdWlEPTB;M{WuN|E{QSEQvF2= MojrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
K5 NGTsXJlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYnJR|UxRTN3LkC4OlEh|ryP NYHjflZnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
TE-10 MlH5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEHsZpVKSzVyPUO0Mlk1OjJizszN MlzmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
NCI-H2141 NF;MUoNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFn3Z4xKSzVyPUO0MlY2OzNizszN NWG3eItERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
KGN NWXqS2hXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlvpTWM2OD1|ND6yOVI1KM7:TR?= M1XHTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
LP-1 Mmr2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGTvU49KSzVyPUOzMlg6ODhizszN MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFc3Oi9pPmPBUmdGWjxxYU6=
NCI-H64 Ml;hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRTN|Lki1PVch|ryP MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFc3Oi9pPmPBUmdGWjxxYU6=
RKO M4nFeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Moi4TWM2OD1|Mz61PVY6KM7:TR?= NYDNPZZ7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
NCI-H526 NVfh[pc6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LN[mlEPTB;M{OuOFk{PiEQvF2= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFc3Oi9pPmPBUmdGWjxxYU6=
GOTO MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXLJR|UxRTN{LkmxNlkh|ryP NWOwNWxLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
Calu-6 NH3BcnBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknETWM2OD1|Mj60O|Q2KM7:TR?= NV32[JRIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
LOUCY NXf1[3l7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXCO5RvUUN3ME2zNk4yOjV|IN88US=> NVzZO25kRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
SK-N-FI MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFn2TYdKSzVyPUOxMlc2OzVizszN MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFc3Oi9pPmPBUmdGWjxxYU6=
SIG-M5 NXLFVm9ET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTYTWM2OD1|MT62PFM{KM7:TR?= NFnyNlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
NKM-1 M4GyTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzWUFZKSzVyPUOxMlE{QTdizszN MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFc3Oi9pPmPBUmdGWjxxYU6=
NCI-SNU-1 NX7rUWFlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVjJR|UxRTNzLkGwOVkh|ryP NWLiSoxrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
NCI-H82 Mn7rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYXJR|UxRTNzLkCxN|Uh|ryP MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFc3Oi9pPmPBUmdGWjxxYU6=
NCI-H510A NYD4UFVQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWnyflcyUUN3ME2zNE4xOzJ7IN88US=> NFn0RZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
ES3 M1KyXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfpWXJKUUN3ME2yPU46PTh{IN88US=> MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFc3Oi9pPmPBUmdGWjxxYU6=
BB30-HNC M1zJdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnrkTWM2OD1{OT65OFg{KM7:TR?= NXfuTnFLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
KM12 NVe5XGF{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVzJR|UxRTJ7Lk[yN|kh|ryP MkT4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
GI-1 M37yOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn\iTWM2OD1{OT6wNVE{KM7:TR?= NYDne|dpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
NOS-1 M{XLNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV3JR|UxRTJ6Lkm3N|Mh|ryP M{LZUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
TE-8 MlyxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHUTWM2OD1{OD65NFgh|ryP MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFc3Oi9pPmPBUmdGWjxxYU6=
TE-9 NFrVcpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTJ6Lke5Olkh|ryP NUnIWFJFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
HL-60 Mk\4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXrMVIlEUUN3ME2yO{46QDZ7IN88US=> M2r4S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
QIMR-WIL MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXT1bXFOUUN3ME2yO{46OTR2IN88US=> MmjrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
KARPAS-299 NVfxVJFmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWj3WppKUUN3ME2yOk45PjR4IN88US=> NFT3Z249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
KURAMOCHI NHPETW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXTJR|UxRTJ4LkiwPFIh|ryP NI\pPWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
BL-41 NEjrZVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWXQeWNPUUN3ME2yOU46PTl5IN88US=> NVjrV4RLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
NCI-H2126 NYHveHR6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4HYcGlEPTB;MkWuOlUzQSEQvF2= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFc3Oi9pPmPBUmdGWjxxYU6=
HOP-62 M4j1emdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHXET5hKSzVyPUK1MlQ1OjVizszN M4\uOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
IST-SL2 M37KdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmPxTWM2OD1{ND61N|Q{KM7:TR?= MlSxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
HH NFXJdINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1\t[mlEPTB;MkSuN|gyQSEQvF2= NVnLXFVGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
LS-513 NGL2XlBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUTJR|UxRTJ|LkWxO|kh|ryP MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFc3Oi9pPmPBUmdGWjxxYU6=
EB-3 M2HFfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFziVmdKSzVyPUKzMlE5OzFizszN MkP2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
ACN NWfBXVlIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVzJR|UxRTJzLkOzPFkh|ryP MkD1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
NOMO-1 Mn6xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXmw[o45UUN3ME2yNU4zODB6IN88US=> M{\QNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
ES8 MlP6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkK5TWM2OD1{MT6wOkDPxE1? M{j2RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
CESS NELNc3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIXFO5ZKSzVyPUKwMlg2PDlizszN MmTaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
BL-70 M{nxUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIjqUVZKSzVyPUKwMlMzPzRizszN M{nwcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
MHH-PREB-1 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{Ozc2lEPTB;MkCuNFM2PiEQvF2= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFc3Oi9pPmPBUmdGWjxxYU6=
BC-1 NXH5e|hET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn7VTWM2OD1zOT6xNVk5KM7:TR?= NVPHcVlGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
LC4-1 NVPydFFMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGTK[nJKSzVyPUG4Mlg4OzRizszN MkK2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
COLO-320-HSR MojyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXjRdo5oUUN3ME2xPE44Pjh6IN88US=> MnLIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
A101D NGPoPVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{nhdmlEPTB;MUiuN|IxQCEQvF2= Mm[4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
BC-3 NHHL[lFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml72TWM2OD1zOD6wN|A2KM7:TR?= NXTyNm1xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
TGW MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmHBTWM2OD1zNz64NVI1KM7:TR?= M1vZV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
JAR NWTQU4I6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnnITWM2OD1zNz6wNVUzKM7:TR?= NWK5eXBuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
HD-MY-Z NUjHXpV4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVTJR|UxRTF4LkiyOFYh|ryP NYryWXNFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
NCI-H1304 Mmq5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYrJR|UxRTF4LkO2NFEh|ryP M1O3fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
OS-RC-2 MkL1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGfv[HhKSzVyPUG1Mlg{QDJizszN MnvvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
OCI-AML2 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnFTWM2OD1zNT62OFgzKM7:TR?= Ml[xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
HCC1599 NVS0NVRyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoOxTWM2OD1zND61PVc2KM7:TR?= NXz1OYE5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
SCC-3 M4XHV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrTTWM2OD1zND6yPVU3KM7:TR?= NHm5T3g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
RPMI-6666 NVv3V484T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFzTTFFKSzVyPUGzMlkyOjFizszN NX;POItwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
MEG-01 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4raWWlEPTB;MUOuPFM4QSEQvF2= Ml\aQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
Raji NXjQZplLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoXWTWM2OD1zMz63PFk1KM7:TR?= MlrSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
RPMI-8402 MlPpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHjJT5lKSzVyPUGzMlYzPjJizszN M4PHcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
GCIY NGfUeXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;aNGlEPTB;MUKuPFYyOyEQvF2= M2XzOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
697 NHy1copIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXT1UIp2UUN3ME2xNk43ODB5IN88US=> MnX2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
D-247MG NXH0S3RMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\sWG1rUUN3ME2xNk4{PTF4IN88US=> MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFc3Oi9pPmPBUmdGWjxxYU6=
NB1 NUe2fJI{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYfJR|UxRTF{LkOzNFgh|ryP MlnOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
COR-L279 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3nhcGlEPTB;MUKuNlkxPyEQvF2= M{fnPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
LB831-BLC NFnxOVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnXYTWM2OD1zMT61OlI1KM7:TR?= Mlr0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
ST486 NXPCNpBoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnlNJp7UUN3ME2xNE4{PTFizszN NGe0cFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
SK-UT-1 NV7QeJZ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTFyLkO1JO69VQ>? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxOFc3Oi9pPmPBUmdGWjxxYU6=
BB65-RCC NWDh[I5VT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1zzb2lEPTB;OT65O|Q6PSEQvF2= NV3wcXNERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTR5NkKvK|5USU6JRWK8M4E,
KARPAS-422 NXn2cFA2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnie2o1UUN3ME25Mlk3OzN4IN88US=> M1qwblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
Becker M3HUWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnvaTWM2OD17LkS2NFgzKM7:TR?= M3zaSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
KS-1 NH7JRlBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTlwNEW3PFUh|ryP NEH5b|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
JiyoyeP-2003 NFXKSYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH6yZ|JKSzVyPUiuOVAzPjRizszN MmjaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ4PjJxJ{7TRW5ITVJ:L3G+
NCCIT NV3NdIZET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVfie|JuUUN3ME23MlU2PDh{IN88US=> M3Lsd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
MRK-nu-1 NGf2W4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknLTWM2OD15LkGyPVY6KM7:TR?= M2jYdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE1PzZ{Lze+V2FPT0WUPD;hQi=>
A3-KAW NGDzcmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF;5U3pKSzVyPUWuO|YyOTZizszN NImzbFk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyPDd4Mj:nQnNCVkeHUkyvZV4>
SK-N-MC M4PvSJFJXFNiYYPzZZk> NVi5NWN7eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uVUNiY3XscJM> NXPtTVM4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW5OVg2QDlpPkG1PVU5PTh7PD;hQi=>
LP-1 MlXPRZBweHSxc3nzJIF{e2G7 NX7vVWJFOzBidV2= MUSzJIhzew>? NV7ueHl5UW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDMVE0yKGOnbHzzJIF1KDNyIIXNJIFnfGW{IEOgbJJ{KHW|aX7nJHRWVkWOIIP0ZYlvcW6pIHL5JIZtd3diY4n0c41mfHK7 MmKxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV7NUi1PFkoRjF3OUW4OVg6RC:jPh?=
LP-1 MUfDfZRwfG:6aXPpeJkh[XO|YYm= M3XyOlIxKHSxIEOwJJVO MVqyOEBpenN? NYqxSod1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVFBvMTDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gc4Yh[2WubDD2bYFjcWyrdImgZZQhOjBidH:gN|AhfU1idILlZZRm\CCob4KgNlQhcHK|IH\vcIxwf2WmIHL5JJdie2ixdYSgcYVie3W{ZXSgZYZ1\XJidH;0ZYwhPzJiaILzJIdzd3e2aDDw[ZJqd2RiYXzhcYFzKGKudXWgZZN{[XlicnXsZZRqfmVidH:gZ49vfHKxbB?= M3f2UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OUW4OVg6Lz5zNUm1PFU5QTxxYU6=
LP-1 MkKzRZBweHSxc3nzJIF{e2G7 MUezNEB2VQ>? NWLaO4tzOS53IHjydy=> M4fNOmlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iTGCtNUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hd2ZiUl7BJJBwdHmvZYLhd4UhOiCyaH;zdIhwe2W{aX7lJFIhdGW4ZXygZZQhOzBidV2gZYZ1\XJiMT61JIhzeyCkeTDpcY12dm:kbH;0eIlv\w>? NUWybnk2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW5OVg2QDlpPkG1PVU5PTh7PD;hQi=>
LP-1 M2PPWGFxd3C2b4Ppd{Bie3OjeR?= NET5VJo{OCC3TR?= NH;XO|M{KGi{cx?= MVPJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEySLUGgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJI9nKE2lbD2xJJBzd3SnaX6gcIV3\WxiYYSgN|AhfU1iYX\0[ZIhOyCqcoOgZpkhcW2vdX7vZoxwfHSrbne= MmPLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV7NUi1PFkoRjF3OUW4OVg6RC:jPh?=
LP-1 NYP4W49XSXCxcITvd4l{KGG|c3H5 NY\2TndxOzBidV2= MmrvN{B1dyB3IHjydy=> NI\ub4lKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJIh2dWGwIFzQMVEh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5ibHX2[Ywhd2ZiY3zlZZZm\CCSQWLQJIF1KDNyIIXNJIFnfGW{IEOgeI8hPSCqcoOgZpkhcW2vdX7vZoxwfHSrbne= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTl3OEW4PUc,OTV7NUi1PFk9N2F-
NCI-H929 MX7BdI9xfG:|aYOgZZN{[Xl? NYXIOWUyOzBidV2= NUjhWoJ4PSCqcoO= MlSxTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCQQ1mtTFkzQSClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDs[ZZmdCCxZjDjcIVifmWmIGDBVnAh[XRiM{CgeW0h[W[2ZYKgOUBpenNiYomgbY1ufW6xYnzveJRqdmd? NGPodlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUm1PFU5QSd-MUW5OVg2QDl:L3G+
NCI-H929 MXLBdI9xfG:|aYOgZZN{[Xl? M1jiblMxKHWP Mom3NU42KGi{cx?= MVzJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKE6FST3IPVI6KGOnbHzzJIF{e2W|c3XkJIF{KG[jc4Sgd4xwfyCvaXfyZZRqdmdiaInw[ZJxcG:|cHjvdplt[XSnZDDSUmEheG:ueX3ldoF{\SB{TzDmc5JuKGG2IEOwJJVOKGGodHXyJFEvPSCqcoOgZpkhcW2vdX7vZoxwfHSrbne= MlHKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV7NUi1PFkoRjF3OUW4OVg6RC:jPh?=
RPM18226 MlH4RZBweHSxc3nzJIF{e2G7 Mk\nN|AhfU1? MoflNU42KGi{cx?= NUHYb2JOUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDSVG0yQDJ{NjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gc4YhWk6DIIDvcJlu\XKjc3WgNkBxcG:|cHjvd4VzcW6nIEKgcIV3\WxiYYSgN|AhfU1iYX\0[ZIhOS53IHjyd{BjgSCrbX31co9jdG:2dHnu[y=> MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTl3OEW4PUc,OTV7NUi1PFk9N2F-
RPM18226 NHLVfHdCeG:ydH;zbZMh[XO|YYm= MYmzNEB2VQ>? NITXW4Y{KGi{cx?= MlXLTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCUUF2xPFIzPiClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25ib3[gUYNtNTFicILveIVqdiCuZY\lcEBifCB|MDD1UUBi\nSncjCzJIhzeyCkeTDpcY12dm:kbH;0eIlv\w>? NEnJPZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUm1PFU5QSd-MUW5OVg2QDl:L3G+
RPM18226 MnLGRZBweHSxc3nzJIF{e2G7 NXTtR2Y3OzBidV2= NECzfFE{KHSxIEWgbJJ{ NGLrPVZKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJIh2dWGwIGLQUVE5OjJ4IHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIxmfmWuIH;mJINt\WG4ZXSgVGFTWCCjdDCzNEB2VSCjZoTldkA{KHSxIEWgbJJ{KGK7IHntcZVvd2Kub4T0bY5o MoC4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV7NUi1PFkoRjF3OUW4OVg6RC:jPh?=
NCI-H929 MUnBdI9xfG:|aYOgZZN{[Xl? M1XUOlMxKHWP MUSzJIhzew>? NGT2b3RKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJIh2dWGwIF7DTU1JQTJ7IHPlcIx{KGG|c3Xzd4VlKGG|IHPoZY5o\XNiaX6gXGlCWCCycn;0[YlvKGyndnXsJIF1KDNyIIXNJIFnfGW{IEOgbJJ{KGK7IHntcZVvd2Kub4T0bY5o M3n2N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OUW4OVg6Lz5zNUm1PFU5QTxxYU6=
NCI-H929 MVTBdI9xfG:|aYOgZZN{[Xl? NWfsRYtGOzBidV2= Mlz5N{BpenN? M4rFe2lv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iTlPJMWg6OjliY3XscJMh[XO|ZYPz[YQh[XNiY3jhcodmeyCrbjDzeZJ3cX[rbjDwdo91\WmwIHzleoVtKGG2IEOwJJVOKGGodHXyJFMhcHK|IHL5JIludXWwb3Lsc5R1cW6p NY[4OYI3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW5OVg2QDlpPkG1PVU5PTh7PD;hQi=>
RPM18226 MnmyRZBweHSxc3nzJIF{e2G7 M4TueFMxKHWP NFmyPVI{KGi{cx?= NWnnW4VPUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDSVG0yQDJ{NjDj[YxteyCjdDCzNEB2VSCjZoTldkA{KGi{czD1d4lv\yCWVV7FUEB{fGGrbnnu[{BjgSCobH;3JIN6fG:vZYTyfS=> NVvTT5lwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW5OVg2QDlpPkG1PVU5PTh7PD;hQi=>
NCI-H929 NX;wcIpYSXCxcITvd4l{KGG|c3H5 NV\l[FVwOzBidV2= M{HnPVEvPSCqcoO= Mom1TY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCQQ1mtTFkzQSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25ib3[gVm5CKHCxbInt[ZJie2ViMjDwbI9{eGixc3XybY5mKDJibHX2[Ywh[XRiM{CgeW0h[W[2ZYKgNU42KGi{czDifUBqdW23bn;icI91fGmwZx?= M{LoZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OUW4OVg6Lz5zNUm1PFU5QTxxYU6=
NCI-H929 M2jkemFxd3C2b4Ppd{Bie3OjeR?= M1S2RlMxKHWP NWLNTW1SOS53IHjydy=> M4\afGlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iTlPJMWg6OjliY3XscJMh[XO|ZYPz[YQh[XNiZHXwbI9{eGixconsZZRqd25ib3[gdHJjKGG2IGOyOFkwXDJ3MjDheEA{OCC3TTDh[pRmeiBzLkWgbJJ{KGK7IHntcZVvd2Kub4T0bY5o NEPJdpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUm1PFU5QSd-MUW5OVg2QDl:L3G+
NCI-H929 MWHDfZRwfG:6aXPpeJkh[XO|YYm= M4f0U|IxKHSxIEOwJJVO NYS4T5F4OTZiaILz NYHHPYFVS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVkOLLVi5Nlkh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKG:oIHPlcIwhfmmjYnnsbZR6KGG2IEKwJJRwKDNyIIXNJJRz\WG2ZXSg[o9zKDF4IHjyd{Bnd2yub4fl[EBjgSC5YYPoc5V1KG2nYYP1doVlKGGodHXyJJRwfGGuIEeyJIhzeyCpcn;3eIgheGW{aX;kJIFt[W2jcjDicJVmKGG|c3H5JJJmdGG2aY\lJJRwKGOxboTyc4w> NIXid5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUm1PFU5QSd-MUW5OVg2QDl:L3G+
NCI-H929 MmHnRZBweHSxc3nzJIF{e2G7 M2DmU|MxKHWP NGfHTYQ{KGi{cx?= MlrpTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCQQ1mtTFkzQSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25ib3[gUYNtNTFicILveIVqdiCuZY\lcEBifCB|MDD1UUBi\nSncjCzJIhzeyCkeTDpcY12dm:kbH;0eIlv\w>? MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTl3OEW4PUc,OTV7NUi1PFk9N2F-
NCI-H929 M{WwdWFxd3C2b4Ppd{Bie3OjeR?= MWezNEB2VQ>? NULuS3hTOyCqcoO= M4HON2lv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iTlPJMWg6OjliY3XscJMh[XO|ZYPz[YQh[XNiY3jhcodmeyCrbjDCZ4wuOiCycn;0[YlvKGyndnXsJIF1KDNyIIXNJIFnfGW{IEOgbJJ{KGK7IHntcZVvd2Kub4T0bY5o NYS2WlBXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW5OVg2QDlpPkG1PVU5PTh7PD;hQi=>
NCI-H929 NVjtXGo2SXCxcITvd4l{KGG|c3H5 NIHvSlA{OCC3TR?= MX6zJIhzew>? MmHxTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCQQ1mtTFkzQSClZXzsd{BifCB|MDD1UUBi\nSncjCzJIhzeyC3c3nu[{BVXU6HTDDzeIFqdmmwZzDifUBndG:5IHP5eI9u\XS{eR?= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTl3OEW4PUc,OTV7NUi1PFk9N2F-
NCI-H929 MmjpRZBweHSxc3nzJIF{e2G7 NHrPV3I{OCC3TR?= NXjDUZRlOS53IHjydy=> M1LufGlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iTlPJMWg6OjliY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJHJPSSCyb3z5cYVz[XOnIEKgdIhwe3Cqb4Pldolv\SB3IHzleoVtKGG2IEOwJJVOKGGodHXyJFEvPSCqcoOgZpkhcW2vdX7vZoxwfHSrbne= M1;hVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OUW4OVg6Lz5zNUm1PFU5QTxxYU6=
NCI-H929 M3LwVmFxd3C2b4Ppd{Bie3OjeR?= NXm2cXVSOzBidV2= MmjUNU42KGi{cx?= M3:4NGlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iTlPJMWg6OjliY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJGhldTJibHX2[Ywh[XRiM{CgeW0h[W[2ZYKgNU42KGi{czDifUBqdW23bn;icI91fGmwZx?= NIXY[Wo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUm1PFU5QSd-MUW5OVg2QDl:L3G+
NCI-H929 NVrscJB7SXCxcITvd4l{KGG|c3H5 NG\ZTHE{OCC3TR?= NFqxSZUyNjViaILz MYPJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKE6FST3IPVI6KGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKG:oIIC1N{Bi[2O3bYXsZZRqd25iYYSgN|AhfU1iYX\0[ZIhOS53IHjyd{BjgSCrbX31co9jdG:2dHnu[y=> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTl3OEW4PUc,OTV7NUi1PFk9N2F-
SK-N-MC MmDxdWhVWyCjc4PhfS=> MoLKdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tUWMh[2WubIO= Mme0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC3NFMoRjJzMEiwO|A{RC:jPh?=
HCT116 MkfVSpVv[3Srb36gZZN{[Xl? NYfBN2xbOzBidH:gOFAhfW2xbD;M M3HRcVI1KGi{cx?= MkXyTY5pcWKrdHnvckBw\iCleXPsbY4hSSCrbjDoeY1idiCKQ2SxNVYh[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5icILveIVqdiCuZY\lcEBifCB|MDD0c{A1OCC3bX;sM2wh[W[2ZYKgNlQhcHK|IHL5JIludXWwb3Lsc5R1cW6pIHHuZYx6e2m| MmrhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC3NFMoRjJzMEiwO|A{RC:jPh?=
HCT116 Mn61SpVv[3Srb36gZZN{[Xl? NXS4NI45OzBidH:gOFAhfW2xbD;M M1XjNVI1KGi{cx?= M3i3WWlvcGmkaYTpc44hd2ZiY4njcIlvKEJiaX6gbJVu[W5iSFPUNVE3KGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIIDyc5RmcW5ibHX2[Ywh[XRiM{CgeI8hPDBidX3vcE9NKGGodHXyJFI1KGi{czDifUBqdW23bn;icI91fGmwZzDhcoFtgXOrcx?= Mn;LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC3NFMoRjJzMEiwO|A{RC:jPh?=
HCT116 NYTIb|NOTnWwY4Tpc44h[XO|YYm= NV\nZo5oOzBidH:gOFAhfW2xbD;M M3XmTVI1KGi{cx?= MXPJcohq[mm2aX;uJI9nKGO7Y3zpckBFOSCrbjDoeY1idiCKQ2SxNVYh[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5icILveIVqdiCuZY\lcEBifCB|MDD0c{A1OCC3bX;sM2wh[W[2ZYKgNlQhcHK|IHL5JIludXWwb3Lsc5R1cW6pIHHuZYx6e2m| MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6MEewN{c,OjFyOEC3NFM9N2F-
HCT116 M1HWNmZ2dmO2aX;uJIF{e2G7 NG\n[pg{OCC2bzC0NEB2dW:uL1y= Ml;pNlQhcHK| NI[4XWhKdmirYnn0bY9vKG:oIFPET|IhcW5iaIXtZY4hUEOWMUG2JINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKHC{b4TlbY4hdGW4ZXygZZQhOzBidH:gOFAhfW2xbD;MJIFnfGW{IEK0JIhzeyCkeTDpcY12dm:kbH;0eIlv\yCjbnHsfZNqew>? NI[5e4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFcxOyd-MkGwPFA4ODN:L3G+
HT-29 MULGeY5kfGmxbjDhd5NigQ>? Ml7DNk42KHSxIESwJJVO NU\rV4tWOjRiaILz NGTJRWZKdmirYnn0bY9vKG:oIILleIlvd2KuYYP0c41iKHC{b4TlbY4hcW5iaIXtZY4hUFRvMkmgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJI9nKGO7Y3zpckBCKGyndnXsJIF1KDJwNTD0c{A1OCC3TTDh[pRmeiB{NDDodpMh[nliaX3teY5w[myxdITpcoc> MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTRzN{SxO{c,OjF2MUe0NVc9N2F-
MCF7 M3LFVWNmdGxiY4njcIUh[XO|YYm= MVqyOEBpenN? MVnD[YxtKGO7Y3zlJIFzemW|dDDpckBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W|c3XkJIF{KGGlY4XteYxifGmxbjDheEBIOi:PIIDoZZNmKGGodHXyJFI1KGi{czD1d4lv\yCycn;wbYRqfW1iaX;kbYRmKGGwZDDCdoRWKHO2YXnubY5oKGK7IH\sc5ch[3m2b33leJJ6 M{DwcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNEG3OFE4Lz5{MUSxO|QyPzxxYU6=
RPMI8226 NE\ZNpNE\WyuIHP5Z4xmKGG|c3H5 Mo\hNlQhcHK| NY\nTWlrS2WubDDjfYNt\SCjcoLld5QhcW5iaIXtZY4hWlCPSUiyNlYh[2WubIOgZZN{\XO|ZXSgZZMh[WOldX31cIF1cW:wIHH0JGczN01icHjhd4Uh[W[2ZYKgNlQhcHK|IIXzbY5oKHC{b4Dp[Il2dSCrb3Tp[IUh[W6mIFLy[HUhe3SjaX7pcoch[nliZnzve{BkgXSxbXX0dpk> NVnRZ2FVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0NVc1OTdpPkKxOFE4PDF5PD;hQi=>
MCF7 MnflR4VtdCCleXPs[UBie3OjeR?= MX2yOEBpenN? NITiS2ZE\WyuIHP5Z4xmKGG{cnXzeEBqdiCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJHMheGijc3WgZ4VtdCCyb4D1cIF1cW:wIHHmeIVzKDJ2IHjyd{B2e2mwZzDwdo9xcWSrdX2gbY9lcWSnIHHu[EBDemSXIIP0ZYlvcW6pIHL5JIZtd3diY4n0c41mfHK7 M1LOWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNEG3OFE4Lz5{MUSxO|QyPzxxYU6=
MCF7 NVnxUZg2S2WubDDjfYNt\SCjc4PhfS=> MYCyOEBpenN? MXzD[YxtKGO7Y3zlJIFzemW|dDDpckBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W|c3XkJIF{KGGlY4XteYxifGmxbjDheEB{fWJvR{GgdIhie2ViYX\0[ZIhOjRiaILzJJV{cW6pIIDyc5Bq\Gm3bTDpc4Rq\GViYX7kJGJz\FVic4ThbY5qdmdiYomg[oxwfyCleYTvcYV1enl? MnntQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2MUe0NVcoRjJzNEG3OFE4RC:jPh?=
RPMI8226 MX7D[YxtKGO7Y3zlJIF{e2G7 MoLmNlQhcHK| NVnNS21RS2WubDDjfYNt\SCjcoLld5QhcW5iaIXtZY4hWlCPSUiyNlYh[2WubIOgZZN{\XO|ZXSgZZMh[WOldX31cIF1cW:wIHH0JJN2[i2JMTDwbIF{\SCjZoTldkAzPCCqcoOgeZNqdmdicILvdIllcXWvIHnv[Ill\SCjbnSgRpJlXSC|dHHpcolv\yCkeTDmcI94KGO7dH;t[ZRzgQ>? NFPMfJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUSxO|QyPyd-MkG0NVc1OTd:L3G+
MCF7 MmfkR4VtdCCleXPs[UBie3OjeR?= MXK4NEB2VQ>? MWGyOEBpenN? MmC2R4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25ib3[gZYN1cX[nbImgdoVxdGmlYYTpcochTE6DIHzleoVtKGG2IEiwJJVOKGGodHXyJFI1KGi{czD1d4lv\yCycn;wbYRqfW1iaX;kbYRmKGGwZDDCdoRWKHO2YXnubY5oKGK7IH\sc5ch[3m2b33leJJ6 MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTRzN{SxO{c,OjF2MUe0NVc9N2F-
MCF7 NEjYcm1HfW6ldHnvckBie3OjeR?= NWDkT4JYOjBidV2= MVyyOEBpenN? Ml35TY5lfWO2aX;uJI9nKHB3Mz3k[ZBmdmSnboSgeJJidnOlcnnweIlwdmGuIHHjeIl3cXS7IHnuJIh2dWGwIF3DSlch[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4Uhd2ZicEKxJHdCTjFibHX2[Ywh[XRiMkCgeW0h[W[2ZYKgNlQhcHK|IHL5JIludXWwb3\seY9z\XOlZX7j[UBie3OjeR?= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTRzN{SxO{c,OjF2MUe0NVc9N2F-
RPMI8226 M{HrOWNmdGxiY4njcIUh[XO|YYm= MYGyOEBpenN? M{HSOGNmdGxiY4njcIUh[XK{ZYP0JIlvKGi3bXHuJHJRVUl6MkK2JINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKFNicHjhd4Uh[2WubDDwc5B2dGG2aX;uJIFnfGW{IEK0JIhzeyC3c3nu[{Bxem:yaXTpeY0hcW:maXTlJIFv\CCEcnTVJJN1[WmwaX7nJIJ6KG[ub4egZ5l1d22ndIL5 NYiySGkyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0NVc1OTdpPkKxOFE4PDF5PD;hQi=>
RPMI8226 MYDD[YxtKGO7Y3zlJIF{e2G7 NHnk[2U5OCC3TR?= Ml3hNlQhcHK| M2nHZ2NmdGxiY4njcIUh[XK{ZYP0JIlvKGi3bXHuJHJRVUl6MkK2JINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBw\iCjY4TpeoVtgSC{ZYDsbYNifGmwZzDEUmEhdGW4ZXygZZQhQDBidV2gZYZ1\XJiMkSgbJJ{KHW|aX7nJJBzd3CrZHn1cUBqd2SrZHWgZY5lKEK{ZGWgd5RicW6rbnegZpkh\myxdzDjfZRwdWW2com= M4XWUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNEG3OFE4Lz5{MUSxO|QyPzxxYU6=
A549 MYfBdI9xfG:|aYOgZZN{[Xl? NWDBPWFuOiC3TR?= MYO0PEBpenN? MY\JcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEF3NEmgZ4VtdHNiYYPz[ZN{\WRiYYOgSG5CKG[{YXft[Y51[XSrb36gZZQhOiC3TTDh[pRmeiB2ODDodpMh[nliYXfhdo9{\SCpZXyg[Yxm[3S{b4Doc5Jme2m| NX3m[2h[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NlM1QTFpPkKzOlI{PDlzPD;hQi=>
Sf9 MYjGeY5kfGmxbjDhd5NigQ>? NFPpS3oyOCCvaX7z NWHQSGJ6UW6qaXLpeIlwdiCxZjDIbZMuPi22YXfn[YQhemWlb33ibY5idnRiaIXtZY4hS0SNMj;jfYNtcW6HIHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2ey2rbn\lZ5Rm\CC|ZkmgZ4VtdHNidYPpcochcGm|dH;u[UBJOSCjczDzeYJ{fHKjdHWgZYZ1\XJiMUCgcYlveyCkeTDsbZF2cWRic3PpcpRqdGyjdHnvckBkd3WwdHnu[{BqdiCycnXz[Y5k\SCxZjDb[4FudWFvM{LQYWFVWCxiSVO1NEA:KDBwMTFOwG0v MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDRzN{W2Okc,OjR2MUe1OlY9N2F-
BJ M2O5PGZ2dmO2aX;uJIF{e2G7 M4nyfVExKHWP MoDCNVAh\GG7cx?= M{TsdnN2eHC{ZYPzbY9vKG:oIIPlcoV{[2WwY3WgbY4hcHWvYX6gRmoh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iY3XscEBvfW2kZYKgZZQhOTBidV2gZYZ1\XJiMUCg[IF6eyCkeTDz[Y5me2OnbnPlJJJmfmW{c3HsJIF{e2G7 MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ6MUm4Okc,OjR4OEG5PFY9N2F-
BJ NYXBS5RFTnWwY4Tpc44h[XO|YYm= MU[xNEB2VQ>? M1;4VFExKGSjeYO= M2DkZWlvcGmkaYTpc44hd2ZiYYThfIliKHSnbHHu[4lm[3Sjc3nhMY12fGG2ZXSgbY4hcHWvYX6gRmoh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iY3XscEBvfW2kZYKgZZQhOTBidV2gZYZ1\XJiMUCg[IF6eyCkeTDz[Y5me2OnbnPlJJJmfmW{c3HsJIF{e2G7 M2q2VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkixPVg3Lz5{NE[4NVk5PjxxYU6=
MCF7 NYDzNm9TTnWwY4Tpc44h[XO|YYm= MkjwNVAhfU1? M2ruZ|ExKG2rboO= NH7Sc2NU\W6|aYTpfoF1cW:wIH;mJIlv\nKjcnXkMYlv\HWlZXSgSG5CKGSjbXHn[UBqdiCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBkd2yxbomg[o9zdWG2aX;uJIF1KDFyIIXNJJBz\XS{ZXH0[YQh\m:{IEGwJI1qdnNiZn;scI94\WRiYomgbZJz[WSrYYTpc44h\m:{IESgbJJ{KG2nYYP1doVlKGGodHXyJFExKGSjeYOgZpkh[3K7c4ThcEB3cW:uZYSgd5RicW6rbnegZY5idHm|aYO= M4nEdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OEWxOVA2Lz5{Nki1NVUxPTxxYU6=
Caco2 MlLaR4VtdCCleXPs[UBie3OjeR?= M3\1SGNmdGxiY4njcIUh[XK{ZYP0JIlvKGi3bXHuJGNi[29{IHPlcIx{KGG|c3Xzd4VlKGG|IHHjZ5VufWyjdHnvckBifCCJMT;TJJBp[XOnIHL5JGhw\WOqc4Sgd5RicW6rbnegZoF{\WRiZnz1c5Jme2OnbnPlJIF{e2G7 MoHHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{MUSyN|EoRjJ6MkG0NlMyRC:jPh?=
HaCaT MXXD[YxtKGO7Y3zlJIF{e2G7 Mn;oR4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gTIFE[VRiY3XscJMh[XO|ZYPz[YQh[XNiYXPjeY12dGG2aX;uJIF1KEdzL2OgdIhie2ViYomgTI9m[2i|dDDzeIFqdmmwZzDiZZNm\CCobIXvdoV{[2WwY3WgZZN{[Xl? NW\GbHdMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyNVQzOzFpPkK4NlE1OjNzPD;hQi=>
HuH7 MlrwR4VtdCCleXPs[UBie3OjeR?= NIjWbXhE\WyuIHP5Z4xmKGG{cnXzeEBqdiCqdX3hckBJfUh5IHPlcIx{KGG|c3Xzd4VlKGG|IHHjZ5VufWyjdHnvckBifCCJMT;TJJBp[XOnIHL5JGhw\WOqc4Sgd5RicW6rbnegZoF{\WRiZnz1c5Jme2OnbnPlJIF{e2G7 M1S0cFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkG0NlMyLz5{OEKxOFI{OTxxYU6=
PC3 MX;D[YxtKGO7Y3zlJIF{e2G7 M2qxXmNmdGxiY4njcIUh[XK{ZYP0JIlvKGi3bXHuJHBEOyClZXzsd{Bie3Onc4Pl[EBieyCjY3P1cZVt[XSrb36gZZQhTzJxTTDwbIF{\SCkeTDIc4VkcHO2IIP0ZYlvcW6pIHLhd4VlKG[udX;y[ZNk\W6lZTDhd5NigQ>? M2DXV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkG0NlMyLz5{OEKxOFI{OTxxYU6=
MDA-MB-231 M2DWVmNmdGxiY4njcIUh[XO|YYm= MnjJR4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDhd5Nme3OnZDDhd{Bi[2O3bYXsZZRqd25iYYSgS|EwWyCyaHHz[UBjgSCKb3XjbJN1KHO2YXnubY5oKGKjc3XkJIZtfW:{ZYPj[Y5k\SCjc4PhfS=> M17HdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkG0NlMyLz5{OEKxOFI{OTxxYU6=
HCT116 MWXD[YxtKGO7Y3zlJIF{e2G7 MkTqR4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gTGNVOTF4IHPlcIx{KGG|c3Xzd4VlKGG|IHHjZ5VufWyjdHnvckBifCCJMT;TJJBp[XOnIHL5JGhw\WOqc4Sgd5RicW6rbnegZoF{\WRiZnz1c5Jme2OnbnPlJIF{e2G7 MlTRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{MUSyN|EoRjJ6MkG0NlMyRC:jPh?=
SK-N-MC MVvxTHRUKGG|c3H5 MYfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= NEPxZ2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW1O|Q{OCd-Mki1OVc1OzB:L3G+
A673 M37GeJFJXFNiYYPzZZk> M3zGfpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCDNkezJINmdGy| MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
DAOY MXnxTHRUKGG|c3H5 NFr1VVNyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiRFHPXUBk\Wyucx?= NV3FZ4piRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
BT-37 NGLBOm1yUFSVIHHzd4F6 M4LYVJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2zO{Bk\Wyucx?= M{LRXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SJ-GBM2 NVn1WmtmeUiWUzDhd5NigQ>? MXTxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?= MnP3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
LAN-5 M3\yfpFJXFNiYYPzZZk> NYnJ[|NYeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFzBUk02KGOnbHzz M1zteVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-MC M2\ocpFJXFNiYYPzZZk> MlPCdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tUWMh[2WubIO= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODF7OUewNkc,OzBzOUm3NFI9N2F-
アッセイ
Methods Test Index PMID
Western blot pT231-tau / pS202-tau / tau ; p-Rb / p-CDK2 / CDK2 / Cyclin D1 / Cyclin A2 / ERα / ERβ/ AIB1 / PELP1 30915013 21834972
Immunofluorescence CDK1 / Smek2 / FUBP1 / Cdc20 ; E2F1 / FASN / Bmi1 / Cyclin D2 / CDK2 / CDK4 24534090 20890301
Growth inhibition assay Cell viability 29996940
体内試験 Roscovitine, at a dose of 50 mg/kg, significantly inhibits growth of The Ewing's sarcoma family of tumors (ESFT) xenografts. [4] Roscovitine enhances the antitumor effect of doxorubicin without increased toxicity with a mechanism that involves cell cycle arrest rather than apoptosis in nude mice bearing established MCF7 xenografts. [5]

お薦めの試験操作(参考用のみ)

キナーゼ試験:[1]
  • Enzymes :

    Kinases activities are assayed at 30 °C in buffer C. Blank values are subtracted from the data and activities calculated as molar amount of phosphate incorporated in protein acceptor during a 10-minute incubation. Controls are performed with appropriate dilutions of DMSO. In a few cases, phosphorylation of the substrate is assessed by autoradiography after SDS/PAGE. p34cdc2/cyclin B is purified from M-phase starfish (M. glacialis) oocytes by affinity chromatography. It is assayed with 1 mg histone Hl/mL, in the presence of 15 μM [γ-32P]ATP (3000 Ci/mmol; 1 mCi/mL) in a final volume of 30 μL. After a 10-minute incubation at 30 °C, 25-μL aliquots of supernatant are spotted onto pieces of Whatman P81 phosphocellulose paper, and, after 20 seconds, the filters are washed five times (for at least 5 minutes each time) in a solution of 10mL phosphoric acid/L water. The wet filters are transferred into 6-mL plastic scintillation vials, 5 mL ACS scintillation fluid is added and the radioactivity measured in a Packard counter. The kinase activity is expressed as molar amount of phosphate incorporated in histone H1 during a 10-minutes incubation or as a percentage of maximal activity. p33cdk2/cyclin A and p33cdk2/cyclinE are reconstituted from extracts of sf9 insect cells infected with various baculoviruses. Cyclins A and E are fusion proteins with glutathione S-transferase and the complexes are purified on glutathione-agarose beads. Kinase activities are assayed with 1 mg/mL histone H1, in the presence of 15 μM [γ-32P]ATP, during 10 minutes, in a final volume of 30 μL, as described for the p34cdc2/cyclin B kinase. p33cdk5/p35 is purified from bovine brain, excluding the Mono S-chromatographic step. The active fractions from the Superose 12 column are pooled and concentrated to a final concentration of approximately 25 μg enzyme/mL. The kinase is assayed with 1 mg/mL histone HI in the presence of 15 μM [γ-32P]ATP, during 10 minutes in a final volume of 30 μL, as described for the p34cdc2/cyclin B kinase. p33cdk5/cyclin D1 is obtained from insect cell lysates. Cdk4 is a fusion protein with glutathione-S-transferase and the active complex is purified on glutathione-agarose beads. Its kinase activity is assayed with purified retinoblastoma protein (complexed with glutathione-S-transferase) in the presence of 15 μM [γ-32P]ATP, in a final volume of 30 μL. After a 15-minute incubation, 30 μL Laemmli sample buffer is added. The phosphorylated substrate is resolved by 10 % SDS/PAGE and analysed by autoradiography by overnight exposure to Hyperfilm MP and densitometry. p33cdk4/cyclinD 2 is obtained from insect cell lysates. It is assayed with purified retinoblastoma protein (complexed with glutathione-S-transferase) in the presence of 15 μM [γ-32P]ATP in a final volume of 30 μL. After a 30-minute incubation, 30 μL Laemmli sample buffer is added. The phosphorylated substrate is resolved by 10% SDS/PAGE and analysed by autoradiography by overnight exposure to Hyperfilm MP and densitometry. MAP kinase erkl (tagged with glutathione-S-transferase), is expressed in bacteria, purified on glutathione-agarose beads and assayed with 1 mg myelin basic protein/ml in the presence of 15 μM [γ-32P]ATP as described above for the p34cdc2cyclin B kinase. His-tagged erkl and erk2 are activated in vitro by mitogen-activated protein kinase kinase, purified (Ni-affinity and Mono Q) and assayed as described above during 10 minutes in a final volume of 30 μL. Protein kinase C isoforms are purified from baculovirus infected sf9 insect cells and assayed with 1 mg/mL protamine sulfate in the presence of 15 μM [γ-32P]ATP, during 10 minutes at 30 °C, in a final volume of 30 μL. Phosphorylated protamine sulfate is recovered on Whatman P81 phosphocellulose paper as described for the cdc2 kinase. The catalytic subunit of cAMP-dependent protein kinase, purified from bovine heart, is assayed with 1 mg histone Hl/ml, in the presence of 15 μM [γ-32P]ATP as described for the p34cdc2/cyclin B kinase. cGMP-dependent protein kinase, purified to homogeneity from bovine tracheal smooth muscle, is assayed with 1 mg histone Hl/mL, in the presence of 15 μM [γ-32P]ATP as described for the p34cdc2/cyclin B kinase. Casein kinase 2 is isolated from rat liver cytosol and assayed with 1 mg casein/mL and 15 μM [γ-32P]ATP. The substrate is spotted on Whatmann 3MM filters and washed with 10% (mass/vol.) trichloroacetic acid. Myosin light chain kinase, purified from chicken gizzard is assayed in the presence of 100 nM calmodulin, 100 μM CaCl2, 50 mM Hepes, 5 mM MgCI,, 1 mM dithiothreitol and 0.1 mg BSA/ml at pH 7.5 using a synthetic peptide based on the smooth-muscle myosin light-chain phosphorylation site and in the presence of 15 μM [γ-32P]ATP, in a final volume of 50 μL. Incorporation of radioactive phosphate is monitored on phosphocellulose filters as described above. ASK-γ, a plant homologue of GSK-3, is expressed as a glutathione-S-transferase fusion protein in Escherichia coli and purified on glutathione-agarose. ASK-γ kinase is assayed, for 10 minutes at 30 °C, with 5 μg myelin basic protein, in the presence of 15 μM [γ-32P]ATP in a final volume of 30 μL. The phosphorylated myelin basic protein is recovered on Whatman P81 phosphocellulose paper as described for the p34cdc2/cyclin B kinase. Insulin receptor tyrosine kinase domain (CIRK-41) is overexpressed in a baculovirus system and purified to homogeneity. Its kinase activity is assayed, for 10 minutes at 30 °C, with 5 μg Raytide, in the presence of 15 μM [γ-32P]ATP, in a final volume of 30 μL. The phosphorylated Raytide is recovered on Whatman P81 phosphocellulose paper as described for the p34cdc2/cyclin B kinase. c-src kinase is purified from infected Sf9 cells. The v-abl kinase is expressed in E. coli and affinity purified on IgG Affigel 10. Both kinases are assayed for 10 minutes at 30 °C, with 5 μg Raytide, in the presence of 15 μM [γ-32P]ATP, in a final volume of 30 μL. The phosphorylated Raytide is recovered on Whatman P81 phosphocellulose paper as described for the p34cdc2/cyclin B kinase.

細胞試験:[1]
  • 細胞株:Leukemia, non-small cell lung cancer, colon cancer, central nervous system cancer, melanoma, ovarian cancer, renal cancer, prostate cancer, breast cancer
  • 濃度: 0.01 - 100 μM
  • 反応時間:48 hours
  • 実験の流れ:60 human tumour cell lines comprising nine tumor types are cultured for 24 hours prior to a 48-hour continuous exposure to 0.01-100 μM roscovitine. A sulforhodaminine B protein assay is used to estimate the cytotoxicity.
動物試験:[4]
  • 動物モデル:A4573 cells are injected s.c. into the right posterior flank of CD1 nu/nu mice.
  • 投薬量:≤50 mg/kg
  • 投与方法:Administered via i.p.

溶解度 (25°C)

体外

体内

左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O
混合させたのち直ちに使用することを推奨します。

10mg/mL

化学情報

分子量 354.45
化学式

C19H26N6O

CAS No. 186692-46-6
Storage 3年 -20°C
2年 -80°C in solvent
Smiles CCC(CO)NC1=NC(=C2C(=N1)N(C=N2)C(C)C)NCC3=CC=CC=C3

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

便利ツール

質量 濃度 体積 分子量

臨床試験

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02649751 Terminated Drug: Roscovitine|Drug: Placebo Cystic Fibrosis University Hospital Brest|ManRos Therapeutics|Cyclacel Pharmaceuticals Inc. February 22 2016 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

大学・企業名を記入してください
名前を記入してください
電子メール・アドレスを記入してください 有効なメールアドレスを入力してください
お問い合わせ内容をご入力ください

よくある質問(FAQ)

質問1:
How can I reconstitute the drug for in vivo studies?

回答
S1153 in 1% DMSO+10% Tween 80+20% N-N-dimethylacetamide+PEG 400 is a clear solution which is okay for injection. And S1153 in 1% DMSO+30% polyethylene glycol+1% Tween 80 at 30mg/ml is a suspension, which is fine for oral gavage.

Tags: Roscovitine (CYC202)を買う | Roscovitine (CYC202) ic50 | Roscovitine (CYC202)供給者 | Roscovitine (CYC202)を購入する | Roscovitine (CYC202)費用 | Roscovitine (CYC202)生産者 | オーダーRoscovitine (CYC202) | Roscovitine (CYC202)化学構造 | Roscovitine (CYC202)分子量 | Roscovitine (CYC202)代理店