Rosiglitazone (BRL 49653)

製品コード:S2556

For research use only.

Rosiglitazone (BRL 49653) is a potent antihyperglycemic agent and a potent thiazolidinedione insulin sensitizer with IC50 of 12, 4 and 9 nM for rat, 3T3-L1 and human adipocytes, respectively. Rosiglitazone is a pure ligand of PPAR-gamma, and has no PPAR-alpha-binding action. Rosiglitazone modulates TRP channels and induces autophagy. Rosiglitazone prevents ferroptosis.

Rosiglitazone (BRL 49653)化学構造

CAS No. 122320-73-4

サイズ 価格(税別)
10mM (1mL in DMSO) JPY 33500
JPY 26400
JPY 38500
JPY 63400

代表番号: 03-5615-9297|電子メール:[email protected]
よく尋ねられる質問

文献中Selleckの製品使用例(51)

製品安全説明書

PPAR阻害剤の選択性比較

相関PPAR製品

生物活性

製品説明 Rosiglitazone (BRL 49653) is a potent antihyperglycemic agent and a potent thiazolidinedione insulin sensitizer with IC50 of 12, 4 and 9 nM for rat, 3T3-L1 and human adipocytes, respectively. Rosiglitazone is a pure ligand of PPAR-gamma, and has no PPAR-alpha-binding action. Rosiglitazone modulates TRP channels and induces autophagy. Rosiglitazone prevents ferroptosis.
ターゲット
PPAR [1]
(Cell-free assay)
TRPC [5] Ferroptosis [6]
体外試験

Rosiglitazone markedly increase phosphorylation of threonine 172 within the α subunit of AMPK, with increased AMP:ATP ratio. Rosiglitazone has been reported to decrease cholesterol synthesis in a number of cell lines in a peroxisome proliferator-activated receptor γ-independent manner. Rosiglitazone activates both α1- and α2-containing AMPK complexes, and this leads to a marked increase in the phosphorylation of acetyl-CoA carboxylase. [1] Rosiglitazone activates PPAR-gamma2 which acts as a dominant inhibitor of osteoblastogenesis in murine bone marrow in vitro.[2] Rosiglitazone increases adiponectin secretion from omental cells up to 2.3-fold higher, whereas secretion from subcutaneous adipose cells is unaffected. [3] Rosiglitazone changes the morphological features and protein profiles of mitochondria in 3T3-L1 adipocytes. [4]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2 NIjMVFdHfW6ldHnvckBie3OjeR?= M{i1OmVn\mWldHn2[UBkd26lZX70doF1cW:wIHHnZYlve3RiaIXtZY4hWFCDUnfhcY1iKGW6cILld5Nm\CCrbjDI[ZBIOiClZXzsd{whTUN3ME2wMlAzKM7:TR?= MofQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV6MEG4NVcoRjF3OECxPFE4RC:jPh?=
CV-1 MUjGeY5kfGmxbjDhd5NigQ>? M2X3emlvKH[rdILvJJRz[W6|Y4LpdJRqd26jbDDhZ5RqfmG2aX;uJI9nKFCncn;4bZNwdWVicILvcIln\XKjdH;yJIFkfGm4YYTl[EBz\WOncITvdkBo[W2vYTCoVHBCWiliZYjwdoV{e2WmIHnuJGNXNTFiY3XscJMtKEWFNUCgQUAxNjB4IN88UU4> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek85PTd4OUC3K|45PTd4OUC3QE9iRg>?
CV-1 NFm3VXBHfW6ldHnvckBie3OjeR?= M2HaZmFkfGm4YYTpc44hd2ZicHXyc5hqe2:vZTDwdo9tcW[ncnH0c5Ih[WO2aY\heIVlKHKnY3XweI9zKGejbX3hJI1m[XO3cnXkJIJ6KGmwZIXjeIlwdiCxZjC1NEUhd2ZibXH4bY12dSCjbHvhcIlv\SCyaH;zdIhifGG|ZTDhZ5Rqfmm2eTygeJJidnOoZXP0bY9vKGG|c3H5JIlvKEOYLUGgZ4VtdHNuIFXDOVAhRSByLkC4PVE{KM7:TT6= NYfseXJCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUizOlYzOCd-OUizOlYzODxxYU6=
3T3-L1 M{DibGZ2dmO2aX;uJIF{e2G7 NHrId4xUfGmvdXzheIlwdiCxZjDh[Ilxd2enbnXzbZMhcW5iM2SzMWwyKGOnbHzzJIl{KGW6cILld5Nm\CCjczDjc45k\W62cnH0bY9vKGWzdXn2ZYxmdnRidH:geIhmKFtiMT2xOGNeKHWydHHr[UBkd3WwdIOgZYZ1\XJidILlZZRu\W62IIfpeIghOC5{IIXnM41NKHS{b3fsbZRigm:wZTygSWMhRSByLkC5JO69VS5? MoLXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB7NU[yNVMoRjFyOUW2NlE{RC:jPh?=
CV-1 NVHTdWFJTnWwY4Tpc44h[XO|YYm= MWXNZZhqdWGuIILldI9zfGW{IHHjeIl3cXS7IHHnZYlve3RiaIXtZY4hWGW{b4jpd49u\SCycn;sbYZmemG2b4KgZYN1cX[jdHXkJJJm[2WydH;yJIdidW2jIFfhcFQh[2irbXXybYMhcW5idILhcpNq\W62bImgeJJidnOoZXP0[YQhS1ZvMTDj[YxteyCkeTDmeY5kfGmxbnHsJIF{e2G7LjygSWM2OCB;IECuNFQ{KM7:TT6= MmfMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5MkC4OVQoRjFzN{KwPFU1RC:jPh?=
COS1 NHjPW2xHfW6ldHnvckBie3OjeR?= M1zoT2Fod26rc4TpZ{Bi[3Srdnn0fUB4[XNiZHX0[ZJucW6nZDDpckBEV1NzIHPlcIx{KHS{YX7z[oVkfGWmIIfpeIghT0GOIEStVHBCWiCpYX3tZUBz\WOncITvdkwhTUN3MDC9JFAvODJzIN88UU4> Mm\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ4MUe5NlQoRjF{NkG3PVI1RC:jPh?=
COS1 NWrjR2g{TnWwY4Tpc44h[XO|YYm= MVPJckB3cXS{bzDh[49vcXO2IHHjeIl3cXS7IITld5Rm\CCob4KgeJJidnOjY4TpeoF1cW:wIHnuJIh2dWGwIGDQRXIh\2GvbXGtS4FtPCClaHnt[ZJq[yCFT2OtNUBk\WyuczygSWM2OCB;IECuNFIh|ryPLh?= NYnZTHFxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK3Nlk3PjhpPkGyO|I6PjZ6PD;hQi=>
COS1 NUPZXW9jTnWwY4Tpc44h[XO|YYm= MXjJckB3cXS{bzD0doFve2GldHn2ZZRqd25ib3[gbJVu[W5icHXyc5hqe2:vZTDwdo9tcW[ncnH0c5Ih[WO2aY\heIVlKHKnY3XweI9zKGejbX3hJI1m[XO3cnXkJIlvKFCSQWKtS2FNPCClaHnt[ZJq[yCFT2OtNUBk\WyuczygSWM2OCB;IECuNFIh|ryPLh?= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjh5M{WxO{c,OTJ6N{O1NVc9N2F-
CV-1 MnPQSpVv[3Srb36gZZN{[Xl? MkX2TY4hfmm2cn:g[ZZidHWjdHnvckBi\2GrboP0JHJZWi2jbIDoZU9RWEGULXfhcY1iKGmwIFPWMVEh[2WubIOgZpkh[2:2cnHud4Zm[3Srb36gZZN{[Xlid3HzJIRmfGW{bXnu[YQtKEWFNUCgQUAxNjN{NTFOwG0v M3rHdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{OUW0NFYyLz5zMkm1OFA3OTxxYU6=
3T3-L1 NFrhOFJHfW6ldHnvckBie3OjeR?= MVvF[oZm[3SrdnWgZ49v[2WwdILheIlwdiCob4Kg[Y5p[W6lZX3lcpQhd2ZiaX7zeYxqdi2rbnT1Z4VlKHS{aXfsfYNmemmmZTDhZ4N2dXWuYYTpc44hcW5iM2SzMWwyKGOnbHzzMEBGSzNyIE2gNE4xOiEQvF2u NGLxNnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNEWwOVY1PCd-MUS1NFU3PDR:L3G+
HepG2 M4L1XWZ2dmO2aX;uJIF{e2G7 NHmwe29G\m[nY4TpeoUh[2:wY3XueJJifGmxbjDh[4FqdnO2IHj1cYFvKFCSQWLnZY1u[SCneIDy[ZN{\WRiaX6gTIVxTzJiY3XscJMtKEWFNUCgQUAxNjB|OTFOwG0v NXLBUFVtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[xNFcyPTBpPkG2NVA4OTVyPD;hQi=>
U2OS NEjJNGxHfW6ldHnvckBie3OjeR?= MUjF[oZm[3Rib36gVHBCWmejbX3hJJRz[W6|YXP0bZZifGmxbjDhZ5Rqfmm2eTDpckBWOk:VIHPlcIx{NCCHQ{WwJF0hOC5yMzFOwG0v MlzOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ|MEC5OFQoRjF4M{CwPVQ1RC:jPh?=
Huh7 NVS0TFl7TnWwY4Tpc44h[XO|YYm= NEe5SHdHfW6ldHnvcoFtKGGldHn2bZR6KGG2IHj1cYFvKFCSQWKg[4FudWFiaX6gTJVpPyClZXzsd{BjgSC2cnHud4FkfGm4YYTpc44h[XO|YYmsJGVEPTBiPTCwMlIzKM7:TT6= M3WxXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4M{[2OlAyLz5zNkO2OlYxOTxxYU6=
Huh7 NGewUplHfW6ldHnvckBie3OjeR?= MkTiSYZn\WO2IH;uJHBRSVJiZ3HtcYEhfHKjboPhZ5RqfmG2aX;uJIFkfGm4aYT5JIlvKEi3aEegZ4VtdHNuIFXDOVAhRSByLkKyJO69VS5? MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjR3MUC4O{c,OTZ2NUGwPFc9N2F-
HEK293 MVjGeY5kfGmxbjDhd5NigQ>? NIHCSZNC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBz\WOncITvdkBqdnSncnHjeIlwdiC5aYToJJN1\XKxaXSgdoVk\XC2b4KgZ49i[3SrdnH0c5IuOSCkeTDFXWZRKGKjc3XkJJJmeG:{dHXyJIdmdmViYYPzZZk> MlXoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
HEK293 M4jxeWZ2dmO2aX;uJIF{e2G7 NGfYR|dC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBz\WOncITvdkBqdnSncnHjeIlwdiC5aYToJJJmfGmwb3nkJHguemWlZYD0c5Ih[WyyaHGgZpkhTVmIUDDiZZNm\CC{ZYDvdpRmeiCpZX7lJIF{e2G7 M3XsSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NkiwNVU6Lz5zNk[4NFE2QTxxYU6=
CV1 NVHGOWRZTnWwY4Tpc44h[XO|YYm= NFzSNJBVemGwc3HjeIl3[XSrb36gc4YhWFCDUnfhcY1iKGmwIFPWNUBk\WyuczygSWM2OCB;IECuNFc3KM7:TT6= NX3NbJR3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[4NlE4PjlpPkG2PFIyPzZ7PD;hQi=>
NIH3T3 NFTLWYRHfW6ldHnvckBie3OjeR?= NH\Fd4xC[3Srdnn0fUBifCCqdX3hckBRWEGUIHfhcY1iKHS{YX7z[oVkfGWmIHnuJG5KUDOWMzDj[YxteyCkeTDseYNq\mW{YYPlJIFkfGm4aYT5JIF{e2G7LDDFR|UxKD1iMD6zNkDPxE1w NE\acpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNki1OFA5PSd-MU[4OVQxQDV:L3G+
CV1 M1nRNGZ2dmO2aX;uJIF{e2G7 M2\hW3Rz[W6|YXP0bZZifGmxbjDv[kBpfW2jbjDQVGFT\2GvbXGgbY4hS1ZzIHPlcIx{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvOzB6IN88UU4> NXvTdWRXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5O|A{QTFpPkG2PVcxOzlzPD;hQi=>
HepG2 MUTGeY5kfGmxbjDhd5NigQ>? NF\4b3VC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVJiZ3HtcYEhcW5iYTDI[ZBIOiClZXzsd{BjgSCSUFHSMWdCVDRidILhcpNi[3SrdnH0bY9vKGG|c3H5MEBGSzVyIE2gNE4yPThizszNMi=> NFvSU2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkm3N|M2QCd-MU[5O|M{PTh:L3G+
HepG2 NWT6[XI{TnWwY4Tpc44h[XO|YYm= NYKxNnlnSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUIHfhcY1iKGmwIFjldGczKGOnbHzzJIJ6KFCSQWKtS2FNPCC2cnHud4FkfGm4YYTpc44h[XO|YYmsJGVEPTBiPTCwMlE2QCEQvF2u NXL4cWhNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5O|k{PDFpPkG2PVc6OzRzPD;hQi=>
CV1 NUi5PJluTnWwY4Tpc44h[XO|YYm= NYH6UVl1SWO2aY\peJkh[XRiaIXtZY4hWFCDUnfhcY1iKGmwIFPWNUBk\WyuczygSWM2OCB;IECuN|A5KM7:TT6= M1rUVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MEC1N|k1Lz5zN{CwOVM6PDxxYU6=
Huh7 NVq1dXVoTnWwY4Tpc44h[XO|YYm= Mk[0RYN1cX[rdImgZZQhcHWvYX6gVHBCWiCpYX3tZUBqdiCKdXi3JINmdGy|IHL5JJRz[W6|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMkKg{txONg>? Ml7BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdyM{SxOFkoRjF5MEO0NVQ6RC:jPh?=
CV1 MmHLSpVv[3Srb36gZZN{[Xl? M4PZbWFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGmwIFPWNUBk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkOwPEDPxE1w NX3MXIJ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexNlk4OjVpPkG3NVI6PzJ3PD;hQi=>
HepG2 MmPmSpVv[3Srb36gZZN{[Xl? MWTB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDpckBJ\XCJMjDj[YxteyCkeTDQVGFTNUeDTESgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yOEKg{txONg>? MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF3N{CxPUc,OTdzNUewNVk9N2F-
HEK293 M1:0W2Z2dmO2aX;uJIF{e2G7 NFm5b2hC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SC{ZXPldJRweiCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGK7IFfBUFQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxKD1iMD6wN|Uh|ryPLh?= NF\XeFQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{K5NlYxPid-MUeyPVI3ODZ:L3G+
HEK293 M3TuU2Z2dmO2aX;uJIF{e2G7 MnTqRYN1cX[rdImgZZQhcHWvYX6gZYRqeG:|ZTD0bZN{fWViUGDBVkBo[W2vYTDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHL5JHBRSVJvR1HMOEB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkOxJO69VS5? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzN2M{O3NUc,OTd|NEOzO|E9N2F-
L6 NV7ZdoluTnWwY4Tpc44h[XO|YYm= M2LSRWVn\mWldDDvckBn[XS2eTDhZ4llKG:6aXTheIlwdiCrbjDyZZQhVDZiY3XscJMtKEWFNUCgQUA2KM7:TT6= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzN2M{O3NUc,OTd|NEOzO|E9N2F-
HepG2/C3A MX7GeY5kfGmxbjDhd5NigQ>? M2T5dmFkfGm4YYTpc44hd2ZiaIXtZY4hWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbj3t[YRq[XSnZDD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliaX6gbJVu[W5iSHXwS|IwSzODIHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiZoXz[YQhT2GuND3MRmQh[nliY3;0doFve2[nY4Tpc44h[XO|YYmsJGVEPTBiPTCwMlAxOzhizszNMi=> NXPRbGpuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NFM3QDhpPkG3OFA{Pjh6PD;hQi=>
CV1 MWfGeY5kfGmxbjDhd5NigQ>? NXXhb3E3OjRiaILz MVvB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDpckBEXjFiY3XscJMh[nliR1HMOEB1emGwc3HjeIl3[XSrb36gZZN{[XliYX\0[ZIhOjRiaILzMEBGSzVyIE2gNE4xOyEQvF2u Ml[xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MEeyNlUoRjF5NUC3NlI2RC:jPh?=
CV1 MnqzSpVv[3Srb36gZZN{[Xl? Mn[3NlQhcHK| NXK1bmFQSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUYXzwbIEhcW5iQ2[xJINmdGy|IHL5JGdCVDRidILhcpNi[3SrdnH0bY9vKGG|c3H5JIFnfGW{IEK0JIhzeyxiRVO1NEA:KDNwNE[g{txONg>? NEfGRY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WwO|IzPSd-MUe1NFczOjV:L3G+
NIH3T3 M3fQfmZ2dmO2aX;uJIF{e2G7 MnizRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW|c3XkJIlvKE6LSEPUN{Bk\WyuczDifUBISUx2IITyZY5{[WO2aY\heIlwdiCjc4PhfUwhTUN3MDC9JFAvOzJizszNMi=> NXXveIE4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NlQ4PzdpPkG3OlI1Pzd5PD;hQi=>
HEK293 M1TuW2Z2dmO2aX;uJIF{e2G7 NV[wR4t2SWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDhVFIh\2WwZTDpcoR2[3Srb36sJGVEPTBiPTCwMlEzKM7:TT6= M1i0VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MEK5NVc3Lz5zOECyPVE4PjxxYU6=
HEK293 M3X2SWZ2dmO2aX;uJIF{e2G7 NGXz[WJC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGK7IFfBUFQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxKD1iMD6xPUDPxE1w NIHrWJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOECyPVE4Pid-MUiwNlkyPzZ:L3G+
CV1 NVPwT5FkTnWwY4Tpc44h[XO|YYm= MlfRRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW|c3XkJIlvKEOYMTDj[YxteyCkeTDy[YNmeHSxcjD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjNyODFOwG0v NEDZcno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOECyPVE4QCd-MUiwNlkyPzh:L3G+
HEK293 NXjtWI1uTnWwY4Tpc44h[XO|YYm= MVfB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiCKRVuyPVMh[2WubIOgZpkhT0GONDD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjB2NTFOwG0v MmLCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{OUG2OFUoRjF6MkmxOlQ2RC:jPh?=
U2OS MoDZSpVv[3Srb36gZZN{[Xl? NULBdJhFSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEhcW5iVULPV{Bk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkCyJO69VS5? MoHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|Mkm3OVEoRjF6M{K5O|UyRC:jPh?=
HepG2 NYO0OnpxTnWwY4Tpc44h[XO|YYm= MmLtRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW|c3XkJIlvKEincFeyJINmdGy|IHL5JGdCVDRidILhcpNi[3SrdnH0bY9vKGG|c3H5MEBGSzVyIE2gNE4zOjNizszNMi=> MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN7NEmwO{c,OTh|OUS5NFc9N2F-
HepG2 NXT1PY5pTnWwY4Tpc44h[XO|YYm= NWXYPXdqSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiZYjwdoV{e2WmIHnuJIh2dWGwIFjldGczKGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDy[YNmeHSxcjD0doFve2GldHn2ZZRqd25iYomgdoVxd3K2ZYKg[4Vv\SCjc4PhfUBz\WyjdHn2[UB1dyClb370do9tNCCHQ{WwJF0hOC5yNTFOwG0v NYLq[GZ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2NlU2PTlpPkG4OlI2PTV7PD;hQi=>
RAW264.7 NIW5cFVHfW6ldHnvckBie3OjeR?= NWLueXlkW3WycILld5Nqd25ib3[gUHBUN0mITj3nZY1u[S2|dHnteYxifGWmIF7PJJBzd2S3Y4Tpc44hcW5ibX;1d4UhWkGZMk[0Mlch[2WubIOsJGlEPTBiPTCxO{42KM7:TT6= M1zhXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OEC5N|I2Lz5zOEiwPVMzPTxxYU6=
CV1 NH\Ne4lHfW6ldHnvckBie3OjeR?= M3;xc2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhS1ZzIHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiZoXz[YQhT2GuND3ERmQh[nlidILhcpNi[3SrdnH0bY9vKGG|c3H5MEBGSzVyIE2gNE4xOzNizszNMi=> NVzGbYk6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi4NlYzODVpPkG4PFI3OjB3PD;hQi=>
CV1 NFHsWppHfW6ldHnvckBie3OjeR?= M17TPGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmGucHjhJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhS1ZzIHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiZoXz[YQhT2GuND3ERmQh[nlidILhcpNi[3SrdnH0bY9vKGG|c3H5MEBGSzVyIE2gN{41PiEQvF2u MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDh{NkKwOUc,OTh6Mk[yNFU9N2F-
HepG2 MV\GeY5kfGmxbjDhd5NigQ>? NULX[Vc2SWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUYXzwbIEhdGmpYX7kJIJqdmSrbneg[I9u[WmwIHX4dJJme3OnZDDpckBpfW2jbjDI[ZBIOiClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKEejbESgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxKD1iMD6wN|kh|ryPLh?= NGTYdlY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEizOVcyQSd-MUi4N|U4OTl:L3G+
HepG2 NHXEV|FHfW6ldHnvckBie3OjeR?= Ml3mRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgcIlo[W6mIHLpcoRqdmdiZH;tZYlvKGW6cILld5Nm\CCrbjDoeY1idiCKZYCgS|Ih[2WubIOgZ48ufHKjboPm[YN1\WRid3n0bEBI[Wx2LVTCSEBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTBiPTCwMlA1KM7:TT6= M{TaXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEWzO|c3Lz5zOUC1N|c4PjxxYU6=
HeLa NUjJVJBWTnWwY4Tpc44h[XO|YYm= M4P3T2Fod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDlfJBz\XO|ZXSgbY4hcHWvYX6gTIVN[SClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKEejbEStSGJFKGG|c3Xzd4VlKGG|IITyZY5{[3KrcITpc45idCCjY4TpeoF1cW:wIHL5JGdidDRicnXzdI9ve2ViZXzlcYVvfC2mcnn2[Y4hdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxKD1iMD6wNVUh|ryPLh?= NH;1cYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUK3OVk3Oyd-MUmyO|U6PjN:L3G+
BL21 MmHoSpVv[3Srb36gZZN{[Xl? MknSSIl{eGyjY3Xt[Y51KG:oIILh[IlwKGyjYnXs[YQhOiiVKT2oNk1j\W68b4nsMZBp\W67bHHtbY5wMS1|LYu0MXsyNDFvZHn0dol1cW9vMj2oOU1u\XSqeXytNk1xcGWweXytc5higm:uLUStfYwqNWW2aH;4fX0ueGinbonsgU1xem:yaX;ubYMh[WOrZDDmdo9uKEeVVD3meZNm\CCqdX3hckBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQlyyNUBk\WyuczDifUB{[2mwdHnscIF1cW:wIIDyc5hqdWm2eTDhd5NigSxiSVO1NEA:KDBwNEWg{txONg>? NHTxRlc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO0PVE4Pid-MUmzOFkyPzZ:L3G+
BHK21 MYLGeY5kfGmxbjDhd5NigQ>? M2Hkd2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCESFuyNUBk\WyuczDhd5Nme3OnZDDhd{BUTUGSIHHjeIl3cXS7IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxKD1iMD60OUDPxE1w MkD4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|NEmxO|YoRjF7M{S5NVc3RC:jPh?=
COS1 MkD1SpVv[3Srb36gZZN{[Xl? MWm0PEBpenN? M4\lS2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gdoVkd22kaX7hcpQhWFCDUnfhcY1iKGW6cILld5Nm\CCrbjDDU3MyKGOnbHzzJINwNWW6cILld5NqdmdiR1HMOEBie3Onc4Pl[EBieyC2cnHud4NzcXC2aX;uZYwh[WO2aY\peJkh[W[2ZYKgOFghcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMEKg{txONg>? M3ixZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUC3PFYyLz5zOUWwO|g3OTxxYU6=
COS1 Mn3LSpVv[3Srb36gZZN{[Xl? NGfPTFdC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iYX\ybYNidiCpcnXlckBud26tZYmgR29UOSClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKG[3c3XkJJlm[XO2IFfhcFQuTEKGIHL5JJRz[W6|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEKg{txONg>? MlzBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3M{C2PFEoRjF7NUOwOlgyRC:jPh?=
U2OS NVH3eXZ1TnWwY4Tpc44h[XO|YYm= MXPB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDlfJBz\XO|ZXSgbY4hXTKRUzDj[YxteyCkeTDseYNq\mW{YYPlJJRz[W6|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEWg{txONg>? NVPtToQ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1O|QxPTZpPkG5OVc1ODV4PD;hQi=>
MCF7 MlnFSpVv[3Srb36gZZN{[Xl? MWmwMlEhfU1? MVKyOEBpenN? MnLQRYN1cX[jdHnvckBw\iCqdX3hckBRWEGULXfhcY1iNWSncHXu[IVvfCC2cnHud4NzcXC2aX;uJIV5eHKnc4Pl[EBqdiCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBRWFKHLYLldI9zfGW{IHflcoUh\XiycnXzd4lwdiCjdDCwMlEhfU1iYX\0[ZIhOjRiaILzJIJ6KGS3YXygcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6KHKnbHH0bZZmKHSxIILvd4lodGm2YYrvcoU> MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTdzOUKzOkc,OTl5MUmyN|Y9N2F-
HepG2 MoHiSpVv[3Srb36gZZN{[Xl? NWLNcJZ[SWexbnnzeEBi[3Srdnn0fUBifCCJQVy0MZRi\2enZDDoeY1idiCSUFHS[4FudWFibHnnZY5lKGKrbnTpcoch\G:vYXnuJIV5eHKnc4Pl[EBqdiCqdX3hckBJ\XCJMjDj[YxteyCjc4Pld5Nm\CCjczDy[YNmeHSxcjD0doFve2GldHn2ZZRqd25iYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yM{mg{txONg>? NFvFcWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUe3OVE3QSd-MUm3O|UyPjl:L3G+
HepG2 NWXveJBqTnWwY4Tpc44h[XO|YYm= NEi5VYFC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSHXwS|Ih[2WubIOgZpkhT0GONDD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjJ{MzFOwG0v NEjzTnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUe4N|Q1PCd-MUm3PFM1PDR:L3G+
COS7 MWHGeY5kfGmxbjDhd5NigQ>? MknrRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDy[YNwdWKrbnHueEBRWEGUZ3HtcYEyKEyERDDlfJBz\XO|ZXSgbY4h[W[{aXPhckBoemWnbjDtc45s\XliQ1;TO{Bk\WyuczDjc4V5eHKnc4PpcochT0GONDDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yMzFOwG0v MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF|OEe2Nkc,OjBzM{i3OlI9N2F-
COS7 NGHVVXBHfW6ldHnvckBie3OjeR?= Ml\LRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDy[YNwdWKrbnHueEBRWEGUZ3HtcYEzKEyERDDlfJBz\XO|ZXSgbY4h[W[{aXPhckBoemWnbjDtc45s\XliQ1;TO{Bk\WyuczDjc4V5eHKnc4PpcochT0GONDDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5zIN88UU4> NGDT[mU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEGzPFc3Oid-MkCxN|g4PjJ:L3G+
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HEK MmGwSpVv[3Srb36gZZN{[Xl? NHjCOWJC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGdCVDRvdHHn[4VlKFCSQWLhcJBp[SClaHnt[ZJq[yC{ZXPldJRweiCneIDy[ZN{\WRiaX6gTGVMKGOnbHzzJIJ6KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IEGwMlU5KM7:TT6= MlrJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB{OUmyNVQoRjJyMkm5NlE1RC:jPh?=
CHO-K1 NH3XXI5HfW6ldHnvckBie3OjeR?= NH3oeY9R[XK2aXHsJIFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hMWxDTCCneIDy[ZN{\WRiaX6gR2hQNUtzIHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiR1HMOEBie3Onc4Pl[EBieyCudXPp[oVz[XOnIHHjeIl3cXS7IHL5JJRz[W6|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMTFOwG0v NVe0SYtURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1Nlc6PjlpPkKwOVI4QTZ7PD;hQi=>
3T3L1 MXvGeY5kfGmxbjDhd5NigQ>? M{HqTlch\GG7cx?= MXzJcoR2[3Srb36gc4Yh[WSrcH;n[Y5me2m|IHnuJI1wfXOnIEPUN2wyKGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHzpdIllKGGlY4XteYxifGmxbjDh[pRmeiB5IHThfZMh[nliT3nsJJJm\CCRIIP0ZYlvcW6p MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODV{N{m2PUc,OjB3Mke5Olk9N2F-
HEK293T NIjCSZlHfW6ldHnvckBie3OjeR?= NYPHV4I6OSC3TR?= MVyyOEBpenN? NFHNT2tR[XK2aXHsJIFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hMWxDTCCneIDy[ZN{\WRiaX6gTGVMOjl|VDDj[YxteyCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4YhemWlZYD0c5IhfHKjboPhZ5RqfmG2aX;uJIF1KDFidV2gZYZ1\XJiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> M2PH[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEOwNlY{Lz5{MUCzNFI3OzxxYU6=
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HEK293 M3GxfWZ2dmO2aX;uJIF{e2G7 NUXKTZdSOTZidH:gNlAhcHK| NYG2S5dTXHKjboPhZ5RqfmG2aX;uJI9nKEejbESt[pV{\WRiaIXtZY4hWFCDUnfhcY1iKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[W[2ZYKgNVYhfG9iMkCgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODR|IN88UU4> MmXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzMki2NFAoRjJzMUK4OlAxRC:jPh?=
HepG2 M4PYbmZ2dmO2aX;uJIF{e2G7 M{HSR|ExKHWvb3yvUC=> MnH4NlQhcHK| NEH1dIRKdmO{ZXHz[UBqdiCpbIXjc5NmKGOxboP1cZB1cW:wIHnuJIh2dWGwIFjldGczKGOnbHzzJIF1KDFyIIXtc4wwVCCjZoTldkAzPCCqcoOgdoVt[XSrdnWgeI8h[2:wdILvcC=> NXmzbVVMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyOVY4PDhpPkKxNlU3PzR6PD;hQi=>
HepG2 M2Hhd2Z2dmO2aX;uJIF{e2G7 NX3ON29kXHKjboPhZ5RqfmG2aX;uJI9nKGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWyR{KgZ4VtdHNiY3:teJJidnOoZXP0[YQhf2m2aDDQVHJGOy2WSz3MeYMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyIE2gNE4xPSEQvF2u MmrFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2NUC0OlgoRjJzNEWwOFY5RC:jPh?=
HepG2 NX7tflhHTnWwY4Tpc44h[XO|YYm= NIfxbmtC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SC{ZXPldJRweiCneIDy[ZN{\WRiaX6gbJVu[W5iSHXwS|Ih[2WubIOgZpkhWFCURT3seYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjBzIN88UU4> MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR6MkS0Okc,OjF2OEK0OFY9N2F-
CV1 NVO0Om01TnWwY4Tpc44h[XO|YYm= M4LQRVQxKGi{cx?= M3rN[2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hMWxDTCCneIDy[ZN{\WRiaX6gR3YyKGOnbHzzJINwNXS{YX7z[oVkfGWmIIfpeIghT2GuNDDh[pRmeiB2MDDodpMh[nlibIXjbYZmemG|ZTDiZZNm\CC2cnHud4FkfGm4YYTpc44h[XO|YYmsJGVEPTBiPTCwMlEh|ryPLh?= NIjzcWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxOVA3Oyd-MkG1NVUxPjN:L3G+
3T3L1 M2TZb2Z2dmO2aX;uJIF{e2G7 MlL4NVAhfU1? M4LKcmlv[3KnYYPlJIlvKGGmaYDvcoVkfGmwIH3SUmEhdGW4ZXzzJIlvKFSQRnHsdIhiNWmwZIXj[YQhdW:3c3WgN3Q{VDFiY3XscJMheHKndILlZZRm\CCjdDCxNEB2VSCkZX\vdoUhXE6ILXHsdIhiKGOqYXzs[Y5o\SC{ZXzheIl3\SC2bzDjc451em:u MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh3MUC4PUc,OjF6NUGwPFk9N2F-
COS7 MVnGeY5kfGmxbjDhd5NigQ>? MmftUY9lfWyjdHnvckBw\iCqdX3hckBRWEGUZ3HtcYEuVEKGIHX4dJJme3OnZDDpckBi\nKrY3HuJIdz\WWwIH3vcotmgSCFT2O3JINmdGy|IHPvMZRz[W6|ZnXjeIVlKHerdHigS4FtPCCjc4Pld5Nm\CCjczDhZ5RqfmG2aX;uJI9nKHS{YX7zZYN1cX[jdHnvckBi[3Srdnn0fUBjgSCudXPp[oVz[XOnIHHzd4F6NCCHQ{WwJF0hOC5yNEOg{txONg>? NYj5UXdRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4O|MxPzBpPkKxPFc{ODdyPD;hQi=>
Sf21 MV3GeY5kfGmxbjDhd5NigQ>? NHqxOZNKdmirYnn0bY9vKG:oIHj1cYFvKEKVRWCg[ZhxemW|c3XkJIlvKHCuYYPtZUBu\W2kcnHu[UB3\XOrY3zld{Bw\iCVZkKxJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiQWTQMYRmeGWwZHXueEBcO0ifdHH1do9kcG:uYYTlJJVxfGGtZTygTWM2OCB;IE[uOEDPxE1w MlrzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NkW2NlMoRjJzOU[1OlI{RC:jPh?=
Sf21 NX\QUlE3TnWwY4Tpc44h[XO|YYm= NWDuWW5xUW6qaXLpeIlwdiCxZjDTdJJi\3WnLVThe4xmgSC{YYSgRpNmeCCneIDy[ZN{\WRiaX6gdIxie22jIH3lcYJz[W6nII\ld4lkdGW|IH;mJHNnOjFiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBCXFBvZHXw[Y5l\W62IGuzTH11[XW{b3Poc4xifGVidYD0ZYtmNCCLQ{WwJF0hPDBizszNMi=> MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl4NU[yN{c,OjF7NkW2NlM9N2F-
COS-1 M1[0SWZ2dmO2aX;uJIF{e2G7 NHTh[owzPCCqcoO= M1fJXWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gR29UNTFiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZT3iZZNm\CCudX3pco9u\XS{aXOgZY5idHm|aYOsJGVEPTBiPTCwMlA1QCEQvF2u NXrqOoV5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwOVExPTRpPkKyNFUyODV2PD;hQi=>
COS1 Mn7nSpVv[3Srb36gZZN{[Xl? MX;CbY5lcW6pIHHm[olvcXS7IITvJIh2dWGwIIfpcIQhfHmyZTDQVGFT\2GvbXGgUGJFKGW6cILld5Nm\CCrbjDDU3MyKGOnbHzzJINwNWW6cILld5NqdmdiR1HMOEBjgSC|Y3nueIltdGG2aX;uJJBzd3irbXn0fUBie3OjeR?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB5ME[wOEc,OjJyN{C2NFQ9N2F-
HepG2 M{Dtd2Z2dmO2aX;uJIF{e2G7 MkDiNlAhcHK| NEnNVWhC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGdCVDRvZoXz[YQhWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDlfJBz\XO|ZXSgbY4hUGWyR{KgZ4VtdHNiYX\0[ZIhOjBiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkCyJO69VS5? MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB6MUmzNkc,OjJyOEG5N|I9N2F-
BL21 DE3 MnjjSpVv[3Srb36gZZN{[Xl? MUnEbZNxdGGlZX3lcpQhd2ZiW{PIYZJwe2mpbHn0ZZpwdmViZoLvcUBPNXSncn3pcoFtKEircz30ZYdo\WRiaIXtZY4hWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzIFTFN{Bk\WyuczDifUB{[2mwdHnscIF1cW:wIIDyc5hqdWm2eTDhd5NigSxiS3mgQUAxNjB5NDFOwG0v MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB6MUmzNkc,OjJyOEG5N|I9N2F-
COS1 M2r6U2Z2dmO2aX;uJIF{e2G7 NHfEXG4zPCCqcoO= MV3UdoFve2GldHn2ZZRqd25ib3[gbJVu[W5iZoXscEBt\W6pdHigVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCFT2OxJINmdGy|IHPvMZRz[W6|ZnXjeIVlKHerdHigVnhT[WyyaHGgZYZ1\XJiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvOTJizszNMi=> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF7N{O5Okc,OjJzOUezPVY9N2F-
MG-63 NITHcFhHfW6ldHnvckBie3OjeR?= Ml;3RYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW|c3XkJIlvKE2JLU[zJINmdGy|IHL5JJJmeG:{dHXyJIdmdmVvYnHz[YQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxKD1iMD6wNVEh|ryPLh?= Ml7UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{MkW2OFEoRjJ{MkK1OlQyRC:jPh?=
293H DA MVjGeY5kfGmxbjDhd5NigQ>? M3yxR|E3KGi{cx?= M1nibGFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKEyERDDpckBpfW2jbjCyPVNJKESDIHPlcIx{KGGodHXyJFE3KGi{czDifUBVWi2IUlXUJIFkfGm4YYTpc44hemWyb4L0[ZIh[XO|YYmsJGVEPTBiPTCwMlAxOjRizszNMi=> NVPnc2JsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1PFI6PzNpPkKyOVgzQTd|PD;hQi=>
MG-63 NIPFTWVHfW6ldHnvckBie3OjeR?= NYq5e3RoOjRiaILz NU\FcJRbVW:mdXzheIlwdiCxZjDmeYxtNWynbnf0bEBpfW2jbjDwV2c2NW[3c3XkJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iTVetOlMh[2WubIOgZ48u\XiycnXzd4lv\yCyR2[tVFIuWFCURTDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGKjc3XkJJRz[W6|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEGxJO69VS5? M3XkeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{K3OFQ5Lz5{MkeyO|Q1QDxxYU6=
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HepG2 MWTGeY5kfGmxbjDhd5NigQ>? NXr4Z21KXHKjboPhZ5RqfmG2aX;uJI9nKEeDTESt[pV{\WRiUGDBVodidW2jIHzp[4Fv\CCkaX7kbY5oKGSxbXHpckB1emGwc3\lZ5Rm\CCrbjDoeY1idiCKZYDHNkBk\WyuczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkGg{txONg>? NYnCWHJ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5N|Q2OzdpPkKyPVM1PTN5PD;hQi=>
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THP1 M4nsNGZ2dmO2aX;uJIF{e2G7 NGnKNGcyODBidV2= M2joUVMhcHK| MkXyWJJidnOjY4TpeoF1cW:wIH;mJHBRSVKpYX3tZUBqdiCqdX3hckBVUFBzIHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJGxRWy2rbnT1Z4VlKEmONjDtVm5CKGW6cILld5Nqd25iYYSgNVAxKHWPIIDy[Ylv[3WkYYTl[EBnd3JiMzDodpMheHKrb4KgeI8hVFCVIHPoZYxt\W6pZTDt[YF{fXKnZDDh[pRmeiBzODDodpMh[nliUmStVGNTKGGwYXz5d4l{KHKnbHH0bZZmKHSxIIXueJJm[XSnZDDjc451em:u MnfjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7M{S1N|coRjJ{OUO0OVM4RC:jPh?=
HepG2 NGLHbIxHfW6ldHnvckBie3OjeR?= MnXiNVAhfU1? NVGzVFNHOjRiaILz MkHyVoVlfWO2aX;uJI9nKGeudXPvd4Uh[2:wc4XtdJRqd25iaX6gbY5{fWyrbj3y[ZNqe3SjboSgbJVu[W5iSHXwS|Ih[2WubIOgZZQhOTBidV2gZYZ1\XJiMkSgbJJ{KGK7IHfseYNwe2Vib4jp[IF{\SCvZYToc4QhcW5icILld4Vv[2Vib3[gNE4yKHWPIH;mJIlve3WuaX6= M2nFV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEK1NlQ1Lz5{M{CyOVI1PDxxYU6=
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HepG2 M{P0b2Z2dmO2aX;uJIF{e2G7 NHO2THozOCCqcoO= MXfUdoFve2GldHn2ZZRqd25ib3[gS2FNPC2odYPl[EBpfW2jbjDQVGFT\2GvbXGgcIlo[W6mIHLpcoRqdmdiZH;tZYlvKGW6cILld5Nm\CCrbjDI[ZBIOiClZXzsd{Bi\nSncjCyNEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yM{mg{txONg>? NHTpS249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G3NVA1PSd-MkOxO|ExPDV:L3G+
L02 MlvYSpVv[3Srb36gZZN{[Xl? NE\oflYzPCCqcoO= M{CwfmFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iNUyERDDlfJBz\XO|ZXSgbY4hcHWvYX6gUFAzKGOnbHzzJINwNWW6cILld5NqdmdicFfMN{1UXjRyLVfBUFQh[W[2ZYKgNlQhcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDiZZNm\CC2cnHud4FkfGm4YYTpc44h[XO|YYmsJGVEPTBiPTCwMlkh|ryPLh?= MmLhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzOE[zNFcoRjJ|MUi2N|A4RC:jPh?=
HEK293 MVrGeY5kfGmxbjDhd5NigQ>? MXS0PEBpenN? MY\B[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHPveJJidnOoZXP0[YQhf2m2aDDQVHJGgDRvVFutcJVkKGG|c3Xzd4VlKGG|IHHjeIl3[XSrb36gc4YhdHWlaX\ldoF{\SCjY4Tpeol1gSCvZXHzeZJm\CCjZoTldkA1QCCqcoOgZpkhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxKD1iNEOuO|Eh|ryPLh?= NVi4[4d5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOlU5PDRpPkKzNlY2QDR2PD;hQi=>
HEK293 NIHtWnVHfW6ldHnvckBie3OjeR?= NVnrV5VvOTBidV2= Mmi3WJJidnOjY4TpeoF1cW:wIH;mJHBRSVKpYX3tZUApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZ48ufHKjboPm[YN1\WRid3n0bEBCWDJvUGDSSUBifCBzMDD1UUBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYm= NU[zZYhkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyO|M2OTlpPkKzNlc{PTF7PD;hQi=>
HEK293 MVXGeY5kfGmxbjDhd5NigQ>? NF7qSXJC\2:waYP0JIF1KGi3bXHuJHBRSVKpYX3tZUBNSkRiZYjwdoV{e2WmIHnuJIh2dWGwIFjFT|I6OyClZXzsd{Bkd3S{YX7z[oVkfGWmIIfpeIghT0GOND3MeYMh[XO|ZYPz[YQh[XNidILhcpNkemmydHnvcoFtKGGldHn2ZZRqd25iYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yMESg{txONg>? M4\z[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|Mki2O|g4Lz5{M{K4Olc5PzxxYU6=
HEK293 MVrGeY5kfGmxbjDhd5NigQ>? NVH1cYt[OjRiaILz Mlr6WJJidnOjY4TpeoF1cW:wIH;mJGdCVDRiRFLEMYZ2e2WmIHj1cYFvKFCSQWLnZY1u[S2OQlSg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyIE2gNE4yKM7:TT6= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ7NEizNEc,OjN{OUS4N|A9N2F-
CMT93 MUDGeY5kfGmxbjDhd5NigQ>? M3;EW|ExKHWP MlzxN|AhdWmwcx?= NEfKNo9KdmS3Y4Tpc44hd2ZiUGDBVk1o[W2vYTDwdo9ud3SncjDhZ5Rqfmm2eTDpckBNWFNvc4TpcZVt[XSnZDDtc5V{\SCFTWS5N{Bk\WyuczDheEAyOCC3TTDwdoVqdmO3YnH0[YQh\m:{IEOwJI1qdnNiYnXmc5JmKEySUz3jbIFtdGWwZ3WgcYVie3W{ZXSgZYZ1\XJiNjDodpMheG:|dDDjbIFtdGWwZ3WgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7 MonhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MU[xPVAoRjJ|NEG2NVkxRC:jPh?=
HepG2 MkOwSpVv[3Srb36gZZN{[Xl? NXfWS4RjSWexbnnzeEBi[3Srdnn0fUBifCCJQVy0MYZ2e2WmIGDQRXJo[W2vYTDNOFY{SSCvdYThcpQhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWyR{KgZ4VtdHNiYomgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yMEKg{txONg>? Ml3IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3MEKyNVIoRjJ|NUCyNlEzRC:jPh?=
HepG2 NIfWSXNHfW6ldHnvckBie3OjeR?= NIfMOlBC\2:waYP0JIFkfGm4aYT5JIF1KEeDTESt[pV{\WRiUGDBVodidW2jIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJIh2dWGwIFjldGczKGOnbHzzJIJ6KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNFEh|ryPLh?= NGHuVFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WwNlIyOid-MkO1NFIzOTJ:L3G+
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HEK293 M{[1eGZ2dmO2aX;uJIF{e2G7 MnflNVAhfU1? MkHwNlQhcHK| NUC1RVJ[XHKjboPhZ5RqfmG2aX;uJI9nKFCSQWKt[4FudWFiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGG2IEGwJJVOKGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[Xl? MkfuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6OUCwPVAoRjJ2OEmwNFkxRC:jPh?=
HEK293 NXi3fmtwTnWwY4Tpc44h[XO|YYm= NW\3dG1lOjRiaILz NIfSOYlC\2:waYP0JIFkfGm4aYT5JIF1KG2xdYPlJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJINwNWW6cILld5Nqdmdid3n0bEBI[Wx2IILldI9zfGW{II\lZ5RweiCjZoTldkAzPCCqcoOgZpkh\HWjbD3seYNq\mW{YYPlJJJmeG:{dHXyJIF{e2G7LDDFR|UxKD1iMD6wN{DPxE1w M{m3Z|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUW1PFg6Lz5{NEm1OVg5QTxxYU6=
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HEK293 NELa[o5HfW6ldHnvckBie3OjeR?= NHHYWmNC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNFEh|ryPLh?= M3j6TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{C1Olg5Lz5{NUOwOVY5QDxxYU6=
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HepaR MnfJSpVv[3Srb36gZZN{[Xl? NIXuT|QyKHWP NHjubYUyKGSjeR?= NHOwXIhC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCrbjDoeY1idiCKZYDhVkBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCKTVfDV|Ih\2WwZTDlfJBz\XO|aX;uJIF1KDFidV2gbY5kfWKjdHXkJIZweiBzIHThfUBjgSCzdXHueIl1[XSrdnWgVGNTKG2ndHjv[EBz\WyjdHn2[UB1dyC3boTy[YF1\WRiY3;ueJJwdA>? Mk\yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUexN|IoRjJ3NEm3NVMzRC:jPh?=
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3T3-L1 NF\VZoZHfW6ldHnvckBie3OjeR?= NUfnWo5tOSC3TR?= MVSxOUBl[Xm| NVrCWZRDSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiaX6gcY92e2ViM2SzMWwyKGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIFPQWFFCKG2UTlGg[ZhxemW|c3nvckBifCBzIIXNJIFnfGW{IEG1JIRigXNiYomgdZVidnSrdHH0bZZmKHKnYXyteIlu\SCSQ2KgcYV1cG:m NV\4RoRZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm4O|g4PjdpPkK5PFc5PzZ5PD;hQi=>
HepG2 NWizWIJMTnWwY4Tpc44h[XO|YYm= MWqyNEBpenN? NYrLUplbXHKjboPhZ5RqfmG2aX;uJI9nKEeDTESteIFo\2WmIHj1cYFvKFCSQWLnZY1u[SCOQlSg[ZhxemW|c3XkJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFIxKGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCzPUDPxE1w NWLwXWJ[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CxPVkzPTNpPkOwNVk6OjV|PD;hQi=>
3T3L1 NF;wXoJHfW6ldHnvckBie3OjeR?= M4HYTVUhfU1? M1HPT2lv\HWldHnvckBw\iCuaYDp[EBi[2O3bYXsZZRqd25iaX6gcY92e2ViM2SzUFEh[2WubIOgZZQhPSC3TTDifUBQcWxiUnXkJG8he3SjaX7pcocu[mG|ZXSgZZN{[Xl? MkWyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzOUmyOVMoRjNyMUm5NlU{RC:jPh?=
3T3L1 Mn;vSpVv[3Srb36gZZN{[Xl? M2HWZ|IhfU1? MkOwTY5lfWO2aX;uJI9nKGyrcHnkJIFk[3WvdXzheIlwdiCrbjDtc5V{\SB|VEPMNUBk\WyuczDheEAzKHWPIHL5JG9qdCCUZXSgU{B{fGGrbnnu[{1j[XOnZDDhd5NigQ>? M361T|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMUm5NlU{Lz5|MEG5PVI2OzxxYU6=
HepG2 M3zuOmZ2dmO2aX;uJIF{e2G7 NYC5dWZlRjFyMDDuUS=> NH3nbWgzOCCqcoO= NUTnbHV1XHKjboPhZ5RqfmG2aX;uJI9nKEeDTESteIFo\2WmIHj1cYFvKFCSQWLnZY1u[SCOQlSg[ZhxemW|c3XkJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGG2IE6xNFAhdk1iYX\0[ZIhOjBiaILzJIlvKHC{ZYPlcoNmKG:oIEWgeW0hWFCDUnfhcY1iKGGwdHHnc45qe3RiR2e5OlYzKGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> MmLQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzOUmyOVMoRjNyMUm5NlU{RC:jPh?=
COS7 MlW1SpVv[3Srb36gZZN{[Xl? NHnZW5ozPCCqcoO= M4XiNGFod26rc4SgZYN1cX[rdImgZZQhemWlb33ibY5idnRiaIXtZY4hT0GOND3ERmQh\nW|ZXSgVHBCWmejbX3hJGxDTCCneIDy[ZN{\WRiaX6gR29UPyClZXzsd{Bi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5|OU[g{txONg>? MoDMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{MkC2NFIoRjNyMkKwOlAzRC:jPh?=
COS7 MXHGeY5kfGmxbjDhd5NigQ>? NYnxSFR7OjRiaILz NXjjXWFPSWexbnnzeEBi[3Srdnn0fUBifCC{ZXPvcYJqdmGwdDDoeY1idiCJQVy0MWRDTCCodYPl[EBRWEGUZ3HtcYEhVEKGIHX4dJJme3OnZDDpckBEV1N5IHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkO5OkDPxE1w M1;mWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMkSxPVA4Lz5|MEK0NVkxPzxxYU6=
HEK293 MX;GeY5kfGmxbjDhd5NigQ>? NXXaUVBVOThiaILz M4XKRmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gS2FNPCCodYPl[EBRWEGUZ3HtcYEuVEKGIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYX\0[ZIhOThiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNVIh|ryPLh?= NIPmR4M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEK5OlY5PSd-M{CyPVY3QDV:L3G+
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HEK293 NVTvXVBWTnWwY4Tpc44h[XO|YYm= NYP2SHI3OThiaILz M2\jUXRz[W6|YXP0bZZifGmxbjDv[kBpfW2jbjDQVGFT\2GvbXGg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDpcoN2[mG2ZXSg[o9zKDF6IHjyd{BjgSCmdXHsJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMEO5JO69VS5? MkXSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVIyNyd-Q3jFUWJNRC:jPh?=
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アッセイ
Methods Test Index PMID
Western blot Cyclin D1 / CDK2 / CDK4 / Bax / Bcl-2 29725405
Immunofluorescence PPARγ / E-cadherin 27145370
Growth inhibition assay Cell viability 29725405
体内試験

Rosiglitazone administration results in significant bone loss, including a decrease in bone volume, trabecular width, and trabecular number and an increase in trabecular spacing. Rosiglitazone also leads to a decrease in bone formation rate, with a simultaneous increase in fat content in the bone marrow. Rosiglitazone decreases the expression of the osteoblast-specific genes Runx2/Cbfa1, Dlx5, and alpha1(I)collagen, whereas the expression of the adipocyte-specific fatty acid binding protein aP2 is increased. [2] Rosiglitazone upregulates gene transcripts encoding mitochondrial proteins in white adipocytes from ob/ob mice accompanied by an increase in mitochondrial mass and changes in mitochondrial structure. [4]

お薦めの試験操作(参考用のみ)

溶解度 (25°C)

体外

体内

左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
4% DMSO+30% PEG 300+5% Tween 80+ddH2O
混合させたのち直ちに使用することを推奨します。

5mg/mL

化学情報

分子量 357.43
化学式

C18H19N3O3S

CAS No. 122320-73-4
Storage 3年 -20°C
2年 -80°C in solvent
Smiles CN(CCOC1=CC=C(C=C1)CC2C(=O)NC(=O)S2)C3=CC=CC=N3

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

便利ツール

質量 濃度 体積 分子量

臨床試験

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02737709 Terminated Drug: Paclitaxel and Carboplatin regimen Non-small Cell Lung Cancer Sun Yat-sen University March 2016 Phase 2
NCT01402076 Completed Drug: Tasimelteon|Drug: Rosiglitazone|Drug: Midazolam Healthy Volunteers Vanda Pharmaceuticals August 2011 Phase 1
NCT01100619 Completed Drug: rosiglitazone|Drug: XL184 Papillary Thyroid Cancer|Follicular Thyroid Cancer|Huerthle Cell Thyroid Cancer|Renal Cell Carcinoma Exelixis April 2010 Phase 1
NCT01415128 Completed Drug: Omeprazole|Drug: Rosiglitazone|Drug: Desipramine Erectile Dysfunction VIVUS LLC April 2010 Phase 1
NCT01376063 Completed Drug: FG-4592 Healthy Adult Subjects FibroGen March 2010 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-08-01)

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

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