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Rosiglitazone (BRL 49653)

製品コード：S2556

For research use only.

Rosiglitazone (BRL 49653) is a potent antihyperglycemic agent and a potent thiazolidinedione insulin sensitizer with IC50 of 12, 4 and 9 nM for rat, 3T3-L1 and human adipocytes, respectively. Rosiglitazone is a pure ligand of PPAR-gamma, and has no PPAR-alpha-binding action. Rosiglitazone modulates TRP channels and induces autophagy. Rosiglitazone prevents ferroptosis.

CAS No. 122320-73-4

サイズ	価格(税別)
10mM (1mL in DMSO)	JPY 33500
25mg	JPY 26400
50mg	JPY 38500
200mg	JPY 63400

代表番号: 03-5615-9297\|電子メール：[email protected] よく尋ねられる質問

文献中Selleckの製品使用例（51）

製品安全説明書

PPAR阻害剤の選択性比較

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Download Inhibitor Catalog

PPAR Signaling Pathway Map

生物活性

製品説明

ターゲット

PPAR ^[1]
(Cell-free assay)

TRPC ^[5]

Ferroptosis ^[6]

体外試験

Rosiglitazone markedly increase phosphorylation of threonine 172 within the α subunit of AMPK, with increased AMP:ATP ratio. Rosiglitazone has been reported to decrease cholesterol synthesis in a number of cell lines in a peroxisome proliferator-activated receptor γ-independent manner. Rosiglitazone activates both α1- and α2-containing AMPK complexes, and this leads to a marked increase in the phosphorylation of acetyl-CoA carboxylase. ^[1] Rosiglitazone activates PPAR-gamma2 which acts as a dominant inhibitor of osteoblastogenesis in murine bone marrow in vitro.^[2] Rosiglitazone increases adiponectin secretion from omental cells up to 2.3-fold higher, whereas secretion from subcutaneous adipose cells is unaffected. ^[3] Rosiglitazone changes the morphological features and protein profiles of mitochondria in 3T3-L1 adipocytes. ^[4]

細胞データ

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

HepG2	NIjMVFdHfW6ldHnvckBie3OjeR?=			M{i1OmVn\mWldHn2[UBkd26lZX70doF1cW:wIHHnZYlve3RiaIXtZY4hWFCDUnfhcY1iKGW6cILld5Nm\CCrbjDI[ZBIOiClZXzsd{whTUN3ME2wMlAzKM7:TR?=	MofQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV6MEG4NVcoRjF3OECxPFE4RC:jPh?=
CV-1	MUjGeY5kfGmxbjDhd5NigQ>?			M2X3emlvKH[rdILvJJRz[W6\|Y4LpdJRqd26jbDDhZ5RqfmG2aX;uJI9nKFCncn;4bZNwdWVicILvcIln\XKjdH;yJIFkfGm4YYTl[EBz\WOncITvdkBo[W2vYTCoVHBCWiliZYjwdoV{e2WmIHnuJGNXNTFiY3XscJMtKEWFNUCgQUAxNjB4IN88UU4>	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek85PTd4OUC3K\|45PTd4OUC3QE9iRg>?
CV-1	NFm3VXBHfW6ldHnvckBie3OjeR?=			M2HaZmFkfGm4YYTpc44hd2ZicHXyc5hqe2:vZTDwdo9tcW[ncnH0c5Ih[WO2aY\heIVlKHKnY3XweI9zKGejbX3hJI1m[XO3cnXkJIJ6KGmwZIXjeIlwdiCxZjC1NEUhd2ZibXH4bY12dSCjbHvhcIlv\SCyaH;zdIhifGG\|ZTDhZ5Rqfmm2eTygeJJidnOoZXP0bY9vKGG\|c3H5JIlvKEOYLUGgZ4VtdHNuIFXDOVAhRSByLkC4PVE{KM7:TT6=	NYfseXJCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUizOlYzOCd-OUizOlYzODxxYU6=
3T3-L1	M{DibGZ2dmO2aX;uJIF{e2G7			NHrId4xUfGmvdXzheIlwdiCxZjDh[Ilxd2enbnXzbZMhcW5iM2SzMWwyKGOnbHzzJIl{KGW6cILld5Nm\CCjczDjc45k\W62cnH0bY9vKGWzdXn2ZYxmdnRidH:geIhmKFtiMT2xOGNeKHWydHHr[UBkd3WwdIOgZYZ1\XJidILlZZRu\W62IIfpeIghOC5{IIXnM41NKHS{b3fsbZRigm:wZTygSWMhRSByLkC5JO69VS5?	MoLXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB7NU[yNVMoRjFyOUW2NlE{RC:jPh?=
CV-1	NVHTdWFJTnWwY4Tpc44h[XO\|YYm=			MWXNZZhqdWGuIILldI9zfGW{IHHjeIl3cXS7IHHnZYlve3RiaIXtZY4hWGW{b4jpd49u\SCycn;sbYZmemG2b4KgZYN1cX[jdHXkJJJm[2WydH;yJIdidW2jIFfhcFQh[2irbXXybYMhcW5idILhcpNq\W62bImgeJJidnOoZXP0[YQhS1ZvMTDj[YxteyCkeTDmeY5kfGmxbnHsJIF{e2G7LjygSWM2OCB;IECuNFQ{KM7:TT6=	MmfMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5MkC4OVQoRjFzN{KwPFU1RC:jPh?=
COS1	NHjPW2xHfW6ldHnvckBie3OjeR?=			M1zoT2Fod26rc4TpZ{Bi[3Srdnn0fUB4[XNiZHX0[ZJucW6nZDDpckBEV1NzIHPlcIx{KHS{YX7z[oVkfGWmIIfpeIghT0GOIEStVHBCWiCpYX3tZUBz\WOncITvdkwhTUN3MDC9JFAvODJzIN88UU4>	Mm\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ4MUe5NlQoRjF{NkG3PVI1RC:jPh?=
COS1	NWrjR2g{TnWwY4Tpc44h[XO\|YYm=			MVPJckB3cXS{bzDh[49vcXO2IHHjeIl3cXS7IITld5Rm\CCob4KgeJJidnOjY4TpeoF1cW:wIHnuJIh2dWGwIGDQRXIh\2GvbXGtS4FtPCClaHnt[ZJq[yCFT2OtNUBk\WyuczygSWM2OCB;IECuNFIh\|ryPLh?=	NYnZTHFxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK3Nlk3PjhpPkGyO\|I6PjZ6PD;hQi=>
COS1	NUPZXW9jTnWwY4Tpc44h[XO\|YYm=			MXjJckB3cXS{bzD0doFve2GldHn2ZZRqd25ib3[gbJVu[W5icHXyc5hqe2:vZTDwdo9tcW[ncnH0c5Ih[WO2aY\heIVlKHKnY3XweI9zKGejbX3hJI1m[XO3cnXkJIlvKFCSQWKtS2FNPCClaHnt[ZJq[yCFT2OtNUBk\WyuczygSWM2OCB;IECuNFIh\|ryPLh?=	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjh5M{WxO{c,OTJ6N{O1NVc9N2F-
CV-1	MnPQSpVv[3Srb36gZZN{[Xl?			MkX2TY4hfmm2cn:g[ZZidHWjdHnvckBi\2GrboP0JHJZWi2jbIDoZU9RWEGULXfhcY1iKGmwIFPWMVEh[2WubIOgZpkh[2:2cnHud4Zm[3Srb36gZZN{[Xlid3HzJIRmfGW{bXnu[YQtKEWFNUCgQUAxNjN{NTFOwG0v	M3rHdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{OUW0NFYyLz5zMkm1OFA3OTxxYU6=
3T3-L1	NFrhOFJHfW6ldHnvckBie3OjeR?=			MVvF[oZm[3SrdnWgZ49v[2WwdILheIlwdiCob4Kg[Y5p[W6lZX3lcpQhd2ZiaX7zeYxqdi2rbnT1Z4VlKHS{aXfsfYNmemmmZTDhZ4N2dXWuYYTpc44hcW5iM2SzMWwyKGOnbHzzMEBGSzNyIE2gNE4xOiEQvF2u	NGLxNnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNEWwOVY1PCd-MUS1NFU3PDR:L3G+
HepG2	M4L1XWZ2dmO2aX;uJIF{e2G7			NHmwe29G\m[nY4TpeoUh[2:wY3XueJJifGmxbjDh[4FqdnO2IHj1cYFvKFCSQWLnZY1u[SCneIDy[ZN{\WRiaX6gTIVxTzJiY3XscJMtKEWFNUCgQUAxNjB\|OTFOwG0v	NXLBUFVtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[xNFcyPTBpPkG2NVA4OTVyPD;hQi=>
U2OS	NEjJNGxHfW6ldHnvckBie3OjeR?=			MUjF[oZm[3Rib36gVHBCWmejbX3hJJRz[W6\|YXP0bZZifGmxbjDhZ5Rqfmm2eTDpckBWOk:VIHPlcIx{NCCHQ{WwJF0hOC5yMzFOwG0v	MlzOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ\|MEC5OFQoRjF4M{CwPVQ1RC:jPh?=
Huh7	NVS0TFl7TnWwY4Tpc44h[XO\|YYm=			NEe5SHdHfW6ldHnvcoFtKGGldHn2bZR6KGG2IHj1cYFvKFCSQWKg[4FudWFiaX6gTJVpPyClZXzsd{BjgSC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlIzKM7:TT6=	M3WxXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4M{[2OlAyLz5zNkO2OlYxOTxxYU6=
Huh7	NGewUplHfW6ldHnvckBie3OjeR?=			MkTiSYZn\WO2IH;uJHBRSVJiZ3HtcYEhfHKjboPhZ5RqfmG2aX;uJIFkfGm4aYT5JIlvKEi3aEegZ4VtdHNuIFXDOVAhRSByLkKyJO69VS5?	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjR3MUC4O{c,OTZ2NUGwPFc9N2F-
HEK293	MVjGeY5kfGmxbjDhd5NigQ>?			NIHCSZNC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCneIDy[ZN{\WRiaX6gTGVMOjl\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnu[JVkfGmxbjDv[kBz\WOncITvdkBqdnSncnHjeIlwdiC5aYToJJN1\XKxaXSgdoVk\XC2b4KgZ49i[3SrdnH0c5IuOSCkeTDFXWZRKGKjc3XkJJJmeG:{dHXyJIdmdmViYYPzZZk>	MlXoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
HEK293	M4jxeWZ2dmO2aX;uJIF{e2G7			NGfYR\|dC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCneIDy[ZN{\WRiaX6gTGVMOjl\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnu[JVkfGmxbjDv[kBz\WOncITvdkBqdnSncnHjeIlwdiC5aYToJJJmfGmwb3nkJHguemWlZYD0c5Ih[WyyaHGgZpkhTVmIUDDiZZNm\CC{ZYDvdpRmeiCpZX7lJIF{e2G7	M3XsSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NkiwNVU6Lz5zNk[4NFE2QTxxYU6=
CV1	NVHGOWRZTnWwY4Tpc44h[XO\|YYm=			NFzSNJBVemGwc3HjeIl3[XSrb36gc4YhWFCDUnfhcY1iKGmwIFPWNUBk\WyuczygSWM2OCB;IECuNFc3KM7:TT6=	NX3NbJR3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[4NlE4PjlpPkG2PFIyPzZ7PD;hQi=>
NIH3T3	NFTLWYRHfW6ldHnvckBie3OjeR?=			NH\Fd4xC[3Srdnn0fUBifCCqdX3hckBRWEGUIHfhcY1iKHS{YX7z[oVkfGWmIHnuJG5KUDOWMzDj[YxteyCkeTDseYNq\mW{YYPlJIFkfGm4aYT5JIF{e2G7LDDFR\|UxKD1iMD6zNkDPxE1w	NE\acpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNki1OFA5PSd-MU[4OVQxQDV:L3G+
CV1	M1nRNGZ2dmO2aX;uJIF{e2G7			M2\hW3Rz[W6\|YXP0bZZifGmxbjDv[kBpfW2jbjDQVGFT\2GvbXGgbY4hS1ZzIHPlcIx{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvOzB6IN88UU4>	NXvTdWRXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5O\|A{QTFpPkG2PVcxOzlzPD;hQi=>
HepG2	MUTGeY5kfGmxbjDhd5NigQ>?			NF\4b3VC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVJiZ3HtcYEhcW5iYTDI[ZBIOiClZXzsd{BjgSCSUFHSMWdCVDRidILhcpNi[3SrdnH0bY9vKGG\|c3H5MEBGSzVyIE2gNE4yPThizszNMi=>	NFvSU2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkm3N\|M2QCd-MU[5O\|M{PTh:L3G+
HepG2	NWT6[XI{TnWwY4Tpc44h[XO\|YYm=			NYKxNnlnSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUIHfhcY1iKGmwIFjldGczKGOnbHzzJIJ6KFCSQWKtS2FNPCC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlE2QCEQvF2u	NXL4cWhNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5O\|k{PDFpPkG2PVc6OzRzPD;hQi=>
CV1	NUi5PJluTnWwY4Tpc44h[XO\|YYm=			NYH6UVl1SWO2aY\peJkh[XRiaIXtZY4hWFCDUnfhcY1iKGmwIFPWNUBk\WyuczygSWM2OCB;IECuN\|A5KM7:TT6=	M1rUVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MEC1N\|k1Lz5zN{CwOVM6PDxxYU6=
Huh7	NVq1dXVoTnWwY4Tpc44h[XO\|YYm=			Mk[0RYN1cX[rdImgZZQhcHWvYX6gVHBCWiCpYX3tZUBqdiCKdXi3JINmdGy\|IHL5JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMkKg{txONg>?	Ml7BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdyM{SxOFkoRjF5MEO0NVQ6RC:jPh?=
CV1	MmHLSpVv[3Srb36gZZN{[Xl?			M4PZbWFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGmwIFPWNUBk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkOwPEDPxE1w	NX3MXIJ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexNlk4OjVpPkG3NVI6PzJ3PD;hQi=>
HepG2	MmPmSpVv[3Srb36gZZN{[Xl?			MWTB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDpckBJ\XCJMjDj[YxteyCkeTDQVGFTNUeDTESgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yOEKg{txONg>?	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF3N{CxPUc,OTdzNUewNVk9N2F-
HEK293	M1:0W2Z2dmO2aX;uJIF{e2G7			NFm5b2hC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SC{ZXPldJRweiCneIDy[ZN{\WRiaX6gTGVMOjl\|IHPlcIx{KGK7IFfBUFQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6wN\|Uh\|ryPLh?=	NF\XeFQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{K5NlYxPid-MUeyPVI3ODZ:L3G+
HEK293	M3TuU2Z2dmO2aX;uJIF{e2G7			MnTqRYN1cX[rdImgZZQhcHWvYX6gZYRqeG:\|ZTD0bZN{fWViUGDBVkBo[W2vYTDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHL5JHBRSVJvR1HMOEB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkOxJO69VS5?	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzN2M{O3NUc,OTd\|NEOzO\|E9N2F-
L6	NV7ZdoluTnWwY4Tpc44h[XO\|YYm=			M2LSRWVn\mWldDDvckBn[XS2eTDhZ4llKG:6aXTheIlwdiCrbjDyZZQhVDZiY3XscJMtKEWFNUCgQUA2KM7:TT6=	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzN2M{O3NUc,OTd\|NEOzO\|E9N2F-
HepG2/C3A	MX7GeY5kfGmxbjDhd5NigQ>?			M2T5dmFkfGm4YYTpc44hd2ZiaIXtZY4hWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbj3t[YRq[XSnZDD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliaX6gbJVu[W5iSHXwS\|IwSzODIHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiZoXz[YQhT2GuND3MRmQh[nliY3;0doFve2[nY4Tpc44h[XO\|YYmsJGVEPTBiPTCwMlAxOzhizszNMi=>	NXPRbGpuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NFM3QDhpPkG3OFA{Pjh6PD;hQi=>
CV1	MWfGeY5kfGmxbjDhd5NigQ>?		NXXhb3E3OjRiaILz	MVvB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDpckBEXjFiY3XscJMh[nliR1HMOEB1emGwc3HjeIl3[XSrb36gZZN{[XliYX\0[ZIhOjRiaILzMEBGSzVyIE2gNE4xOyEQvF2u	Ml[xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MEeyNlUoRjF5NUC3NlI2RC:jPh?=
CV1	MnqzSpVv[3Srb36gZZN{[Xl?		Mn[3NlQhcHK\|	NXK1bmFQSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUYXzwbIEhcW5iQ2[xJINmdGy\|IHL5JGdCVDRidILhcpNi[3SrdnH0bY9vKGG\|c3H5JIFnfGW{IEK0JIhzeyxiRVO1NEA:KDNwNE[g{txONg>?	NEfGRY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WwO\|IzPSd-MUe1NFczOjV:L3G+
NIH3T3	M3fQfmZ2dmO2aX;uJIF{e2G7			MnizRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW\|c3XkJIlvKE6LSEPUN{Bk\WyuczDifUBISUx2IITyZY5{[WO2aY\heIlwdiCjc4PhfUwhTUN3MDC9JFAvOzJizszNMi=>	NXXveIE4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NlQ4PzdpPkG3OlI1Pzd5PD;hQi=>
HEK293	M1TuW2Z2dmO2aX;uJIF{e2G7			NV[wR4t2SWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDhVFIh\2WwZTDpcoR2[3Srb36sJGVEPTBiPTCwMlEzKM7:TT6=	M1i0VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MEK5NVc3Lz5zOECyPVE4PjxxYU6=
HEK293	M3X2SWZ2dmO2aX;uJIF{e2G7			NGXz[WJC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCneIDy[ZN{\WRiaX6gTGVMOjl\|IHPlcIx{KGK7IFfBUFQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6xPUDPxE1w	NIHrWJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOECyPVE4Pid-MUiwNlkyPzZ:L3G+
CV1	NVPwT5FkTnWwY4Tpc44h[XO\|YYm=			MlfRRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW\|c3XkJIlvKEOYMTDj[YxteyCkeTDy[YNmeHSxcjD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjNyODFOwG0v	NEDZcno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOECyPVE4QCd-MUiwNlkyPzh:L3G+
HEK293	NXjtWI1uTnWwY4Tpc44h[XO\|YYm=			MVfB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiCKRVuyPVMh[2WubIOgZpkhT0GONDD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjB2NTFOwG0v	MmLCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{OUG2OFUoRjF6MkmxOlQ2RC:jPh?=
U2OS	MoDZSpVv[3Srb36gZZN{[Xl?			NULBdJhFSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEhcW5iVULPV{Bk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkCyJO69VS5?	MoHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh\|Mkm3OVEoRjF6M{K5O\|UyRC:jPh?=
HepG2	NYO0OnpxTnWwY4Tpc44h[XO\|YYm=			MmLtRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW\|c3XkJIlvKEincFeyJINmdGy\|IHL5JGdCVDRidILhcpNi[3SrdnH0bY9vKGG\|c3H5MEBGSzVyIE2gNE4zOjNizszNMi=>	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN7NEmwO{c,OTh\|OUS5NFc9N2F-
HepG2	NXT1PY5pTnWwY4Tpc44h[XO\|YYm=			NWXYPXdqSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiZYjwdoV{e2WmIHnuJIh2dWGwIFjldGczKGOnbHzzJIF{e2W\|c3XkJIF{KGmwZIXjeIlwdiCxZjDy[YNmeHSxcjD0doFve2GldHn2ZZRqd25iYomgdoVxd3K2ZYKg[4Vv\SCjc4PhfUBz\WyjdHn2[UB1dyClb370do9tNCCHQ{WwJF0hOC5yNTFOwG0v	NYLq[GZ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2NlU2PTlpPkG4OlI2PTV7PD;hQi=>
RAW264.7	NIW5cFVHfW6ldHnvckBie3OjeR?=			NWLueXlkW3WycILld5Nqd25ib3[gUHBUN0mITj3nZY1u[S2\|dHnteYxifGWmIF7PJJBzd2S3Y4Tpc44hcW5ibX;1d4UhWkGZMk[0Mlch[2WubIOsJGlEPTBiPTCxO{42KM7:TT6=	M1zhXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OEC5N\|I2Lz5zOEiwPVMzPTxxYU6=
CV1	NH\Ne4lHfW6ldHnvckBie3OjeR?=			M3;xc2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhS1ZzIHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiZoXz[YQhT2GuND3ERmQh[nlidILhcpNi[3SrdnH0bY9vKGG\|c3H5MEBGSzVyIE2gNE4xOzNizszNMi=>	NVzGbYk6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi4NlYzODVpPkG4PFI3OjB3PD;hQi=>
CV1	NFHsWppHfW6ldHnvckBie3OjeR?=			M17TPGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmGucHjhJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhS1ZzIHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiZoXz[YQhT2GuND3ERmQh[nlidILhcpNi[3SrdnH0bY9vKGG\|c3H5MEBGSzVyIE2gN{41PiEQvF2u	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDh{NkKwOUc,OTh6Mk[yNFU9N2F-
HepG2	MV\GeY5kfGmxbjDhd5NigQ>?			NULX[Vc2SWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUYXzwbIEhdGmpYX7kJIJqdmSrbneg[I9u[WmwIHX4dJJme3OnZDDpckBpfW2jbjDI[ZBIOiClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKEejbESgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR\|UxKD1iMD6wN\|kh\|ryPLh?=	NGTYdlY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEizOVcyQSd-MUi4N\|U4OTl:L3G+
HepG2	NHXEV\|FHfW6ldHnvckBie3OjeR?=			Ml3mRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgcIlo[W6mIHLpcoRqdmdiZH;tZYlvKGW6cILld5Nm\CCrbjDoeY1idiCKZYCgS\|Ih[2WubIOgZ48ufHKjboPm[YN1\WRid3n0bEBI[Wx2LVTCSEBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlA1KM7:TT6=	M{TaXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEWzO\|c3Lz5zOUC1N\|c4PjxxYU6=
HeLa	NUjJVJBWTnWwY4Tpc44h[XO\|YYm=			M4P3T2Fod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDlfJBz\XO\|ZXSgbY4hcHWvYX6gTIVN[SClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKEejbEStSGJFKGG\|c3Xzd4VlKGG\|IITyZY5{[3KrcITpc45idCCjY4TpeoF1cW:wIHL5JGdidDRicnXzdI9ve2ViZXzlcYVvfC2mcnn2[Y4hdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR\|UxKD1iMD6wNVUh\|ryPLh?=	NH;1cYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUK3OVk3Oyd-MUmyO\|U6PjN:L3G+
BL21	MmHoSpVv[3Srb36gZZN{[Xl?			MknSSIl{eGyjY3Xt[Y51KG:oIILh[IlwKGyjYnXs[YQhOiiVKT2oNk1j\W68b4nsMZBp\W67bHHtbY5wMS1\|LYu0MXsyNDFvZHn0dol1cW9vMj2oOU1u\XSqeXytNk1xcGWweXytc5higm:uLUStfYwqNWW2aH;4fX0ueGinbonsgU1xem:yaX;ubYMh[WOrZDDmdo9uKEeVVD3meZNm\CCqdX3hckBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQlyyNUBk\WyuczDifUB{[2mwdHnscIF1cW:wIIDyc5hqdWm2eTDhd5NigSxiSVO1NEA:KDBwNEWg{txONg>?	NHTxRlc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO0PVE4Pid-MUmzOFkyPzZ:L3G+
BHK21	MYLGeY5kfGmxbjDhd5NigQ>?			M2Hkd2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCESFuyNUBk\WyuczDhd5Nme3OnZDDhd{BUTUGSIHHjeIl3cXS7IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD60OUDPxE1w	MkD4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl\|NEmxO\|YoRjF7M{S5NVc3RC:jPh?=
COS1	MkD1SpVv[3Srb36gZZN{[Xl?		MWm0PEBpenN?	M4\lS2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gdoVkd22kaX7hcpQhWFCDUnfhcY1iKGW6cILld5Nm\CCrbjDDU3MyKGOnbHzzJINwNWW6cILld5NqdmdiR1HMOEBie3Onc4Pl[EBieyC2cnHud4NzcXC2aX;uZYwh[WO2aY\peJkh[W[2ZYKgOFghcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMEKg{txONg>?	M3ixZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUC3PFYyLz5zOUWwO\|g3OTxxYU6=
COS1	Mn3LSpVv[3Srb36gZZN{[Xl?			NGfPTFdC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iYX\ybYNidiCpcnXlckBud26tZYmgR29UOSClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKG[3c3XkJJlm[XO2IFfhcFQuTEKGIHL5JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEKg{txONg>?	MlzBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3M{C2PFEoRjF7NUOwOlgyRC:jPh?=
U2OS	NVH3eXZ1TnWwY4Tpc44h[XO\|YYm=			MXPB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDlfJBz\XO\|ZXSgbY4hXTKRUzDj[YxteyCkeTDseYNq\mW{YYPlJJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEWg{txONg>?	NVPtToQ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1O\|QxPTZpPkG5OVc1ODV4PD;hQi=>
MCF7	MlnFSpVv[3Srb36gZZN{[Xl?	MWmwMlEhfU1?	MVKyOEBpenN?	MnLQRYN1cX[jdHnvckBw\iCqdX3hckBRWEGULXfhcY1iNWSncHXu[IVvfCC2cnHud4NzcXC2aX;uJIV5eHKnc4Pl[EBqdiCqdX3hckBOS0Z5IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnu[JVkfGmxbjDv[kBRWFKHLYLldI9zfGW{IHflcoUh\XiycnXzd4lwdiCjdDCwMlEhfU1iYX\0[ZIhOjRiaILzJIJ6KGS3YXygcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6KHKnbHH0bZZmKHSxIILvd4lodGm2YYrvcoU>	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTdzOUKzOkc,OTl5MUmyN\|Y9N2F-
HepG2	MoHiSpVv[3Srb36gZZN{[Xl?			NWLNcJZ[SWexbnnzeEBi[3Srdnn0fUBifCCJQVy0MZRi\2enZDDoeY1idiCSUFHS[4FudWFibHnnZY5lKGKrbnTpcoch\G:vYXnuJIV5eHKnc4Pl[EBqdiCqdX3hckBJ\XCJMjDj[YxteyCjc4Pld5Nm\CCjczDy[YNmeHSxcjD0doFve2GldHn2ZZRqd25iYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yM{mg{txONg>?	NFvFcWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUe3OVE3QSd-MUm3O\|UyPjl:L3G+
HepG2	NWXveJBqTnWwY4Tpc44h[XO\|YYm=			NEi5VYFC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSHXwS\|Ih[2WubIOgZpkhT0GONDD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjJ{MzFOwG0v	NEjzTnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUe4N\|Q1PCd-MUm3PFM1PDR:L3G+
COS7	MWHGeY5kfGmxbjDhd5NigQ>?			MknrRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDy[YNwdWKrbnHueEBRWEGUZ3HtcYEyKEyERDDlfJBz\XO\|ZXSgbY4h[W[{aXPhckBoemWnbjDtc45s\XliQ1;TO{Bk\WyuczDjc4V5eHKnc4PpcochT0GONDDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yMzFOwG0v	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF\|OEe2Nkc,OjBzM{i3OlI9N2F-
COS7	NGHVVXBHfW6ldHnvckBie3OjeR?=			Ml\LRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDy[YNwdWKrbnHueEBRWEGUZ3HtcYEzKEyERDDlfJBz\XO\|ZXSgbY4h[W[{aXPhckBoemWnbjDtc45s\XliQ1;TO{Bk\WyuczDjc4V5eHKnc4PpcochT0GONDDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5zIN88UU4>	NGDT[mU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEGzPFc3Oid-MkCxN\|g4PjJ:L3G+
HEK	M1v1Z2Z2dmO2aX;uJIF{e2G7			M3T5ZWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gS2FNPC22YXfn[YQhWFCDUnfhcY1iKGOqaX3ldolkKHKnY3XweI9zKGW6cILld5Nm\CCrbjDISWsh[2WubIOgZpkhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6wN\|Uh\|ryPLh?=	NUfvS\|ZuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCyPVkzOTRpPkKwNlk6OjF2PD;hQi=>
HEK	MmGwSpVv[3Srb36gZZN{[Xl?			NHjCOWJC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGdCVDRvdHHn[4VlKFCSQWLhcJBp[SClaHnt[ZJq[yC{ZXPldJRweiCneIDy[ZN{\WRiaX6gTGVMKGOnbHzzJIJ6KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IEGwMlU5KM7:TT6=	MlrJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB{OUmyNVQoRjJyMkm5NlE1RC:jPh?=
CHO-K1	NH3XXI5HfW6ldHnvckBie3OjeR?=			NH3oeY9R[XK2aXHsJIFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hMWxDTCCneIDy[ZN{\WRiaX6gR2hQNUtzIHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiR1HMOEBie3Onc4Pl[EBieyCudXPp[oVz[XOnIHHjeIl3cXS7IHL5JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMTFOwG0v	NVe0SYtURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1Nlc6PjlpPkKwOVI4QTZ7PD;hQi=>
3T3L1	MXvGeY5kfGmxbjDhd5NigQ>?		M{HqTlch\GG7cx?=	MXzJcoR2[3Srb36gc4Yh[WSrcH;n[Y5me2m\|IHnuJI1wfXOnIEPUN2wyKGOnbHzzJIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIHzpdIllKGGlY4XteYxifGmxbjDh[pRmeiB5IHThfZMh[nliT3nsJJJm\CCRIIP0ZYlvcW6p	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODV{N{m2PUc,OjB3Mke5Olk9N2F-
HEK293T	NIjCSZlHfW6ldHnvckBie3OjeR?=	NYPHV4I6OSC3TR?=	MVyyOEBpenN?	NFHNT2tR[XK2aXHsJIFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hMWxDTCCneIDy[ZN{\WRiaX6gTGVMOjl\|VDDj[YxteyCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4YhemWlZYD0c5IhfHKjboPhZ5RqfmG2aX;uJIF1KDFidV2gZYZ1\XJiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=>	M2PH[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEOwNlY{Lz5{MUCzNFI3OzxxYU6=
CV1	NX;iOFg5TnWwY4Tpc44h[XO\|YYm=			MV7UdoFve2GldHn2ZZRqd25ib3[gS4FtPC2odYPl[EBpfW2jbjDQVGFT\2GvbXGgSG5CKGKrbnTpcoch\G:vYXnuJIV5eHKnc4Pl[EBqdiCjZoLpZ4FvKGe{ZXXuJI1wdmuneTDDWlEh[2WubIOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR\|UxKD1iMD6xJO69VS5?	M1vKdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUGyO\|g1Lz5{MUGxNlc5PDxxYU6=
HEK293	M3GxfWZ2dmO2aX;uJIF{e2G7		NUXKTZdSOTZidH:gNlAhcHK\|	NYG2S5dTXHKjboPhZ5RqfmG2aX;uJI9nKEejbESt[pV{\WRiaIXtZY4hWFCDUnfhcY1iKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[W[2ZYKgNVYhfG9iMkCgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODR\|IN88UU4>	MmXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzMki2NFAoRjJzMUK4OlAxRC:jPh?=
HepG2	M4PYbmZ2dmO2aX;uJIF{e2G7	M{HSR\|ExKHWvb3yvUC=>	MnH4NlQhcHK\|	NEH1dIRKdmO{ZXHz[UBqdiCpbIXjc5NmKGOxboP1cZB1cW:wIHnuJIh2dWGwIFjldGczKGOnbHzzJIF1KDFyIIXtc4wwVCCjZoTldkAzPCCqcoOgdoVt[XSrdnWgeI8h[2:wdILvcC=>	NXmzbVVMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyOVY4PDhpPkKxNlU3PzR6PD;hQi=>
HepG2	M2Hhd2Z2dmO2aX;uJIF{e2G7			NX3ON29kXHKjboPhZ5RqfmG2aX;uJI9nKGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWyR{KgZ4VtdHNiY3:teJJidnOoZXP0[YQhf2m2aDDQVHJGOy2WSz3MeYMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyIE2gNE4xPSEQvF2u	MmrFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2NUC0OlgoRjJzNEWwOFY5RC:jPh?=
HepG2	NX7tflhHTnWwY4Tpc44h[XO\|YYm=			NIfxbmtC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SC{ZXPldJRweiCneIDy[ZN{\WRiaX6gbJVu[W5iSHXwS\|Ih[2WubIOgZpkhWFCURT3seYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjBzIN88UU4>	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR6MkS0Okc,OjF2OEK0OFY9N2F-
CV1	NVO0Om01TnWwY4Tpc44h[XO\|YYm=		M4LQRVQxKGi{cx?=	M3rN[2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hMWxDTCCneIDy[ZN{\WRiaX6gR3YyKGOnbHzzJINwNXS{YX7z[oVkfGWmIIfpeIghT2GuNDDh[pRmeiB2MDDodpMh[nlibIXjbYZmemG\|ZTDiZZNm\CC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlEh\|ryPLh?=	NIjzcWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxOVA3Oyd-MkG1NVUxPjN:L3G+
3T3L1	M2TZb2Z2dmO2aX;uJIF{e2G7	MlL4NVAhfU1?		M4LKcmlv[3KnYYPlJIlvKGGmaYDvcoVkfGmwIH3SUmEhdGW4ZXzzJIlvKFSQRnHsdIhiNWmwZIXj[YQhdW:3c3WgN3Q{VDFiY3XscJMheHKndILlZZRm\CCjdDCxNEB2VSCkZX\vdoUhXE6ILXHsdIhiKGOqYXzs[Y5o\SC{ZXzheIl3\SC2bzDjc451em:u	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh3MUC4PUc,OjF6NUGwPFk9N2F-
COS7	MVnGeY5kfGmxbjDhd5NigQ>?			MmftUY9lfWyjdHnvckBw\iCqdX3hckBRWEGUZ3HtcYEuVEKGIHX4dJJme3OnZDDpckBi\nKrY3HuJIdz\WWwIH3vcotmgSCFT2O3JINmdGy\|IHPvMZRz[W6\|ZnXjeIVlKHerdHigS4FtPCCjc4Pld5Nm\CCjczDhZ5RqfmG2aX;uJI9nKHS{YX7zZYN1cX[jdHnvckBi[3Srdnn0fUBjgSCudXPp[oVz[XOnIHHzd4F6NCCHQ{WwJF0hOC5yNEOg{txONg>?	NYj5UXdRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4O\|MxPzBpPkKxPFc{ODdyPD;hQi=>
Sf21	MV3GeY5kfGmxbjDhd5NigQ>?			NHqxOZNKdmirYnn0bY9vKG:oIHj1cYFvKEKVRWCg[ZhxemW\|c3XkJIlvKHCuYYPtZUBu\W2kcnHu[UB3\XOrY3zld{Bw\iCVZkKxJINmdGy\|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiQWTQMYRmeGWwZHXueEBcO0ifdHH1do9kcG:uYYTlJJVxfGGtZTygTWM2OCB;IE[uOEDPxE1w	MlrzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NkW2NlMoRjJzOU[1OlI{RC:jPh?=
Sf21	NX\QUlE3TnWwY4Tpc44h[XO\|YYm=			NWDuWW5xUW6qaXLpeIlwdiCxZjDTdJJi\3WnLVThe4xmgSC{YYSgRpNmeCCneIDy[ZN{\WRiaX6gdIxie22jIH3lcYJz[W6nII\ld4lkdGW\|IH;mJHNnOjFiY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBCXFBvZHXw[Y5l\W62IGuzTH11[XW{b3Poc4xifGVidYD0ZYtmNCCLQ{WwJF0hPDBizszNMi=>	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl4NU[yN{c,OjF7NkW2NlM9N2F-
COS-1	M1[0SWZ2dmO2aX;uJIF{e2G7		NHTh[owzPCCqcoO=	M1fJXWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gR29UNTFiY3XscJMh[W[2ZYKgNlQhcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZT3iZZNm\CCudX3pco9u\XS{aXOgZY5idHm\|aYOsJGVEPTBiPTCwMlA1QCEQvF2u	NXrqOoV5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwOVExPTRpPkKyNFUyODV2PD;hQi=>
COS1	Mn7nSpVv[3Srb36gZZN{[Xl?			MX;CbY5lcW6pIHHm[olvcXS7IITvJIh2dWGwIIfpcIQhfHmyZTDQVGFT\2GvbXGgUGJFKGW6cILld5Nm\CCrbjDDU3MyKGOnbHzzJINwNWW6cILld5NqdmdiR1HMOEBjgSC\|Y3nueIltdGG2aX;uJJBzd3irbXn0fUBie3OjeR?=	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB5ME[wOEc,OjJyN{C2NFQ9N2F-
HepG2	M{Dtd2Z2dmO2aX;uJIF{e2G7		MkDiNlAhcHK\|	NEnNVWhC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGdCVDRvZoXz[YQhWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDlfJBz\XO\|ZXSgbY4hUGWyR{KgZ4VtdHNiYX\0[ZIhOjBiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkCyJO69VS5?	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB6MUmzNkc,OjJyOEG5N\|I9N2F-
BL21 DE3	MnjjSpVv[3Srb36gZZN{[Xl?			MUnEbZNxdGGlZX3lcpQhd2ZiW{PIYZJwe2mpbHn0ZZpwdmViZoLvcUBPNXSncn3pcoFtKEircz30ZYdo\WRiaIXtZY4hWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDlfJBz\XO\|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzIFTFN{Bk\WyuczDifUB{[2mwdHnscIF1cW:wIIDyc5hqdWm2eTDhd5NigSxiS3mgQUAxNjB5NDFOwG0v	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB6MUmzNkc,OjJyOEG5N\|I9N2F-
COS1	M2r6U2Z2dmO2aX;uJIF{e2G7		NHfEXG4zPCCqcoO=	MV3UdoFve2GldHn2ZZRqd25ib3[gbJVu[W5iZoXscEBt\W6pdHigVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCFT2OxJINmdGy\|IHPvMZRz[W6\|ZnXjeIVlKHerdHigVnhT[WyyaHGgZYZ1\XJiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvOTJizszNMi=>	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF7N{O5Okc,OjJzOUezPVY9N2F-
MG-63	NITHcFhHfW6ldHnvckBie3OjeR?=			Ml;3RYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW\|c3XkJIlvKE2JLU[zJINmdGy\|IHL5JJJmeG:{dHXyJIdmdmVvYnHz[YQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6wNVEh\|ryPLh?=	Ml7UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{MkW2OFEoRjJ{MkK1OlQyRC:jPh?=
293H DA	MVjGeY5kfGmxbjDhd5NigQ>?		M3yxR\|E3KGi{cx?=	M1nibGFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKEyERDDpckBpfW2jbjCyPVNJKESDIHPlcIx{KGGodHXyJFE3KGi{czDifUBVWi2IUlXUJIFkfGm4YYTpc44hemWyb4L0[ZIh[XO\|YYmsJGVEPTBiPTCwMlAxOjRizszNMi=>	NVPnc2JsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1PFI6PzNpPkKyOVgzQTd\|PD;hQi=>
MG-63	NIPFTWVHfW6ldHnvckBie3OjeR?=		NYq5e3RoOjRiaILz	NU\FcJRbVW:mdXzheIlwdiCxZjDmeYxtNWynbnf0bEBpfW2jbjDwV2c2NW[3c3XkJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iTVetOlMh[2WubIOgZ48u\XiycnXzd4lv\yCyR2[tVFIuWFCURTDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGKjc3XkJJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEGxJO69VS5?	M3XkeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{K3OFQ5Lz5{MkeyO\|Q1QDxxYU6=
Ac2F	NXjpdpE6TnWwY4Tpc44h[XO\|YYm=	MljHNE4yKHSxIEGwJJVO	MWW2JIhzew>?	NWPKdGhYSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiaX6gdoF1KEGlMl[gZ4VtdHNiYYPz[ZN{\WRiYYOgcJVkcW[ncnHz[UBi[3Srdnn0fUBifCByLkGgeI8hOTBidV2gZYZ1\XJiNjDodpMh[nlicnXwc5J1\XJiZ3Xu[UBie3OjeR?=	NIm0Tm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkixPVE6OCd-MkK4NVkyQTB:L3G+
HepG2	MWTGeY5kfGmxbjDhd5NigQ>?			NXr4Z21KXHKjboPhZ5RqfmG2aX;uJI9nKEeDTESt[pV{\WRiUGDBVodidW2jIHzp[4Fv\CCkaX7kbY5oKGSxbXHpckB1emGwc3\lZ5Rm\CCrbjDoeY1idiCKZYDHNkBk\WyuczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkGg{txONg>?	NYnCWHJ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5N\|Q2OzdpPkKyPVM1PTN5PD;hQi=>
THP1	NEP6SoNHfW6ldHnvckBie3OjeR?=	MmW2NVAxKHWP	MUizJIhzew>?	NYHBc3JVXHKjboPhZ5RqfmG2aX;uJI9nKFCSQWLnZY1u[SCrbjDoeY1idiCWSGCxJINmdGy\|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKEySUz3pcoR2[2WmIF3DVFEhdVKQQTDlfJBz\XO\|aX;uJIF1KDFyMDD1UUBxemWrbnP1ZoF1\WRiZn;yJFMhcHK\|IIDybY9zKHSxIFzQV{BkcGGubHXu[4UhdWWjc4Xy[YQh[W[2ZYKgNVghcHK\|IHL5JHJVNVCFUjDhcoFtgXOrczDy[YxifGm4ZTD0c{B2dnS{ZXH0[YQh[2:wdILvcC=>	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl\|NEWzO{c,OjJ7M{S1N\|c9N2F-
THP1	M4nsNGZ2dmO2aX;uJIF{e2G7	NGnKNGcyODBidV2=	M2joUVMhcHK\|	MkXyWJJidnOjY4TpeoF1cW:wIH;mJHBRSVKpYX3tZUBqdiCqdX3hckBVUFBzIHPlcIx{KGG\|c3Xzd4VlKGG\|IHTlZ5Jm[XOnIHnuJGxRWy2rbnT1Z4VlKEmONjDtVm5CKGW6cILld5Nqd25iYYSgNVAxKHWPIIDy[Ylv[3WkYYTl[EBnd3JiMzDodpMheHKrb4KgeI8hVFCVIHPoZYxt\W6pZTDt[YF{fXKnZDDh[pRmeiBzODDodpMh[nliUmStVGNTKGGwYXz5d4l{KHKnbHH0bZZmKHSxIIXueJJm[XSnZDDjc451em:u	MnfjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7M{S1N\|coRjJ{OUO0OVM4RC:jPh?=
HepG2	NGLHbIxHfW6ldHnvckBie3OjeR?=	MnXiNVAhfU1?	NVGzVFNHOjRiaILz	MkHyVoVlfWO2aX;uJI9nKGeudXPvd4Uh[2:wc4XtdJRqd25iaX6gbY5{fWyrbj3y[ZNqe3SjboSgbJVu[W5iSHXwS\|Ih[2WubIOgZZQhOTBidV2gZYZ1\XJiMkSgbJJ{KGK7IHfseYNwe2Vib4jp[IF{\SCvZYToc4QhcW5icILld4Vv[2Vib3[gNE4yKHWPIH;mJIlve3WuaX6=	M2nFV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MEK1NlQ1Lz5{M{CyOVI1PDxxYU6=
HepG2	NUmyUW9oTnWwY4Tpc44h[XO\|YYm=	MXixNEB2VQ>?	NITvfnYzPCCqcoO=	MXrS[YR2[3Srb36gc4Yh\2y3Y3;z[UBkd26\|dX3weIlwdiCrbjDpcpN2dGmwLYLld4l{fGGwdDDoeY1idiCKZYDHNkBk\WyuczDheEAyOCC3TTDh[pRmeiB{NDDodpMh[nliZ3z1Z49{\SCxeHnkZZNmKG2ndHjv[EBqdiCycnXz[Y5k\SCxZjCyNk4zKG2PIH;mJIdtfWOxc3W=	Ml3iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMkWyOFQoRjJ\|MEK1NlQ1RC:jPh?=
HepG2	M{P0b2Z2dmO2aX;uJIF{e2G7		NHO2THozOCCqcoO=	MXfUdoFve2GldHn2ZZRqd25ib3[gS2FNPC2odYPl[EBpfW2jbjDQVGFT\2GvbXGgcIlo[W6mIHLpcoRqdmdiZH;tZYlvKGW6cILld5Nm\CCrbjDI[ZBIOiClZXzsd{Bi\nSncjCyNEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yM{mg{txONg>?	NHTpS249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G3NVA1PSd-MkOxO\|ExPDV:L3G+
L02	MlvYSpVv[3Srb36gZZN{[Xl?		NE\oflYzPCCqcoO=	M{CwfmFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iNUyERDDlfJBz\XO\|ZXSgbY4hcHWvYX6gUFAzKGOnbHzzJINwNWW6cILld5NqdmdicFfMN{1UXjRyLVfBUFQh[W[2ZYKgNlQhcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDiZZNm\CC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlkh\|ryPLh?=	MmLhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzOE[zNFcoRjJ\|MUi2N\|A4RC:jPh?=
HEK293	MVrGeY5kfGmxbjDhd5NigQ>?		MXS0PEBpenN?	MY\B[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHPveJJidnOoZXP0[YQhf2m2aDDQVHJGgDRvVFutcJVkKGG\|c3Xzd4VlKGG\|IHHjeIl3[XSrb36gc4YhdHWlaX\ldoF{\SCjY4Tpeol1gSCvZXHzeZJm\CCjZoTldkA1QCCqcoOgZpkhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iNEOuO\|Eh\|ryPLh?=	NVi4[4d5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOlU5PDRpPkKzNlY2QDR2PD;hQi=>
HEK293	NIHtWnVHfW6ldHnvckBie3OjeR?=	NVnrV5VvOTBidV2=		Mmi3WJJidnOjY4TpeoF1cW:wIH;mJHBRSVKpYX3tZUApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZ48ufHKjboPm[YN1\WRid3n0bEBCWDJvUGDSSUBifCBzMDD1UUBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYm=	NU[zZYhkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyO\|M2OTlpPkKzNlc{PTF7PD;hQi=>
HEK293	MVXGeY5kfGmxbjDhd5NigQ>?			NF7qSXJC\2:waYP0JIF1KGi3bXHuJHBRSVKpYX3tZUBNSkRiZYjwdoV{e2WmIHnuJIh2dWGwIFjFT\|I6OyClZXzsd{Bkd3S{YX7z[oVkfGWmIIfpeIghT0GOND3MeYMh[XO\|ZYPz[YQh[XNidILhcpNkemmydHnvcoFtKGGldHn2ZZRqd25iYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yMESg{txONg>?	M4\z[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|Mki2O\|g4Lz5{M{K4Olc5PzxxYU6=
HEK293	MVrGeY5kfGmxbjDhd5NigQ>?		NVH1cYt[OjRiaILz	Mlr6WJJidnOjY4TpeoF1cW:wIH;mJGdCVDRiRFLEMYZ2e2WmIHj1cYFvKFCSQWLnZY1u[S2OQlSg[ZhxemW\|c3XkJIlvKEiHS{K5N{Bk\WyuczDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyIE2gNE4yKM7:TT6=	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ7NEizNEc,OjN{OUS4N\|A9N2F-
CMT93	MUDGeY5kfGmxbjDhd5NigQ>?	M3;EW\|ExKHWP	MlzxN\|AhdWmwcx?=	NEfKNo9KdmS3Y4Tpc44hd2ZiUGDBVk1o[W2vYTDwdo9ud3SncjDhZ5Rqfmm2eTDpckBNWFNvc4TpcZVt[XSnZDDtc5V{\SCFTWS5N{Bk\WyuczDheEAyOCC3TTDwdoVqdmO3YnH0[YQh\m:{IEOwJI1qdnNiYnXmc5JmKEySUz3jbIFtdGWwZ3WgcYVie3W{ZXSgZYZ1\XJiNjDodpMheG:\|dDDjbIFtdGWwZ3WgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7	MonhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MU[xPVAoRjJ\|NEG2NVkxRC:jPh?=
HepG2	MkOwSpVv[3Srb36gZZN{[Xl?			NXfWS4RjSWexbnnzeEBi[3Srdnn0fUBifCCJQVy0MYZ2e2WmIGDQRXJo[W2vYTDNOFY{SSCvdYThcpQhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWyR{KgZ4VtdHNiYomgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yMEKg{txONg>?	Ml3IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3MEKyNVIoRjJ\|NUCyNlEzRC:jPh?=
HepG2	NIfWSXNHfW6ldHnvckBie3OjeR?=			NIfMOlBC\2:waYP0JIFkfGm4aYT5JIF1KEeDTESt[pV{\WRiUGDBVodidW2jIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJIh2dWGwIFjldGczKGOnbHzzJIJ6KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNFEh\|ryPLh?=	NGHuVFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WwNlIyOid-MkO1NFIzOTJ:L3G+
L6	NHrXXW5HfW6ldHnvckBie3OjeR?=		NV\1e\|k1OjRiaILz	MUHJcoR2[3Srb36gc4Yh\2y3Y3;z[UB2eHSja3WgbY4hemG2IFy2JINmdGy\|IID1cJNm\CC5aYToJGMyPC2mZX;4fUBodHWlb4PlJIFnfGW{IEK0JIhzeyCrbjDwdoV{\W6lZTDv[kBqdnO3bHnuMEBGSzVyIE2gOE41QSEQvF2u	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl7Mki2Nkc,OjN7OUK4OlI9N2F-
L6	NXzGWYZQTnWwY4Tpc44h[XO\|YYm=		NUHWRZNOOTZiaILz	MlHCTY5kemWjc3WgbY4hOi2dM1jdMYRmd3i7Z3z1Z49{\SC3cIThb4UhcW5icnH0JGw3KGOnbHzzJIFnfGW{IEG2JIhzeyCkeTDzZ4lvfGmubHH0bY9vKGOxdX70bY5oKGGwYXz5d4l{NCCLQ{WwJF0hPC56IN88UU4>	MkTTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUO3N\|goRjJ2OEGzO\|M5RC:jPh?=
HEK293	M{[1eGZ2dmO2aX;uJIF{e2G7	MnflNVAhfU1?	MkHwNlQhcHK\|	NUC1RVJ[XHKjboPhZ5RqfmG2aX;uJI9nKFCSQWKt[4FudWFiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gTGVMOjl\|IHPlcIx{KGG2IEGwJJVOKGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[Xl?	MkfuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6OUCwPVAoRjJ2OEmwNFkxRC:jPh?=
HEK293	NXi3fmtwTnWwY4Tpc44h[XO\|YYm=		NW\3dG1lOjRiaILz	NIfSOYlC\2:waYP0JIFkfGm4aYT5JIF1KG2xdYPlJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJINwNWW6cILld5Nqdmdid3n0bEBI[Wx2IILldI9zfGW{II\lZ5RweiCjZoTldkAzPCCqcoOgZpkh\HWjbD3seYNq\mW{YYPlJJJmeG:{dHXyJIF{e2G7LDDFR\|UxKD1iMD6wN{DPxE1w	M{m3Z\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUW1PFg6Lz5{NEm1OVg5QTxxYU6=
DU145	MmK2R5l1d3SxeHnjbZR6KGG\|c3H5		M4fPOlQ5KGi{cx?=	NVrwT5NQS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hTFVzNEWgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEG2JO69VS5?	NVXrXYRORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5PVYyPDNpPkK0PVk3OTR\|PD;hQi=>
PC3	NWi3cYhNS3m2b4TvfIlkcXS7IHHzd4F6		M1TJcVQ5KGi{cx?=	NV7NNotNS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWEN\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB{MD6zJO69VS5?	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl7NkG0N{c,OjR7OU[xOFM9N2F-
MDA-MB-231	NYPuT401T3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NIPF[YUzPCCqcoO=	MVzHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNSGEuVUJvMkOxJINmdGy\|IHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB3LkKzJO69VS5?	NX6xZVJzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyO\|gzOzZpPkK1Nlc5OjN4PD;hQi=>
HEK293	NELa[o5HfW6ldHnvckBie3OjeR?=			NHHYWmNC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNFEh\|ryPLh?=	M3j6TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{C1Olg5Lz5{NUOwOVY5QDxxYU6=
HepG2	MUfGeY5kfGmxbjDhd5NigQ>?		M3;FPVIxKGi{cx?=	MULUdoFve2GldHn2ZZRqd25ib3[gVHBCWiC2cnHud4Zm[3SnZDDpckBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjCyNEBpenNiYomgVHBCWi2udXPp[oVz[XOnIHHzd4F6NCCHQ{WwJF0hOS54IN88UU4>	MlTTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{ezNFQoRjJ3NEO3N\|A1RC:jPh?=
HEK293	MoLhSpVv[3Srb36gZZN{[Xl?		M3\TN\|I1KGi{cx?=	MXHUdoFve2GldHn2ZZRqd25ib3[gVHBCWmejbX3hJEh2dmuwb4fuJI9zcWerbjmg[ZhxemW\|c3XkJIlvKEiHS{K5N{Bk\WyuczDpcoN2[mG2ZXSg[o9zKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYm=	M3;TXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NESyN\|IzLz5{NUS0NlMzOjxxYU6=
HepG2	MmC5SpVv[3Srb36gZZN{[Xl?			NYLFUYJkSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBISUx2LWDQRXJo[W2vYTDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIHj1cYFvKEincFeyJINmdGy\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMEO5JO69VS5?	NHXpfGQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS2NlI5OSd-MkW0OlIzQDF:L3G+
HepG2	MWPGeY5kfGmxbjDhd5NigQ>?		M33DbFIxKGi{cx?=	M2DESGFod26rc4SgZYN1cX[rdImgZZQhT0GOND3EUmEh[mmwZHnu[{Bld22jaX6g[pV{\WRiaIXtZY4hWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDlfJBz\XO\|ZXSgbY4hcHWvYX6gTIVxTzJiY3XscJMh[XO\|ZYPz[YQh[XNicnXj[ZB1d3JidILhcpNi[3SrdnH0bY9vKGmwY4XiZZRm\CCob4KgNlAhcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMESg{txONg>?	NH3ObZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS5O\|E{Oid-MkW0PVcyOzJ:L3G+
HepaR	NVK1O\|g5TnWwY4Tpc44h[XO\|YYm=	NYjhSYtXOSC3TR?=	NFHXPZcyKGSjeR?=	M3XVdWFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGmwIHj1cYFvKEincHHSJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKE[DQmCxJIdmdmViZYjwdoV{e2mxbjDheEAyKHWPIHnuZ5Vj[XSnZDDmc5IhOSCmYYmgZpkheXWjboTpeIF1cX[nIGDDVkBu\XSqb3SgdoVt[XSrdnWgeI8hfW62cnXheIVlKGOxboTyc4w>	MkHDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUexN\|IoRjJ3NEm3NVMzRC:jPh?=
HepaR	M164XGZ2dmO2aX;uJIF{e2G7	MWKxJJVO	M1qxcVEh\GG7	NV\CS3lYSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiaX6gbJVu[W5iSHXwZXIh[2WubIOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iQV7HVHRNPCCpZX7lJIV5eHKnc4Ppc44h[XRiMTD1UUBqdmO3YnH0[YQh\m:{IEGg[IF6KGK7IIH1ZY51cXSjdHn2[UBRS1JibXX0bI9lKHKnbHH0bZZmKHSxIIXueJJm[XSnZDDjc451em:u	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR7N{GzNkc,OjV2OUexN\|I9N2F-
HepaR	MnfJSpVv[3Srb36gZZN{[Xl?	NIXuT\|QyKHWP	NHjubYUyKGSjeR?=	NHOwXIhC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCrbjDoeY1idiCKZYDhVkBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCKTVfDV\|Ih\2WwZTDlfJBz\XO\|aX;uJIF1KDFidV2gbY5kfWKjdHXkJIZweiBzIHThfUBjgSCzdXHueIl1[XSrdnWgVGNTKG2ndHjv[EBz\WyjdHn2[UB1dyC3boTy[YF1\WRiY3;ueJJwdA>?	Mk\yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUexN\|IoRjJ3NEm3NVMzRC:jPh?=
C2C12	M{fyW2Z2dmO2aX;uJIF{e2G7	NYrmS3cxOzBidV2=	MXOyJIhzew>?	MUPJcoR2[3Srb36gc4YhOi2mZX;4fU1cO0ifLXfseYNwe2VidYD0ZYtmKGmwIH3veZNmKEN{Q{GyJINmdGy\|IHH0JFMxKHWPIHHmeIVzKDJiaILzJIJ6KGyrcYXp[EB{[2mwdHnscIF1cW:wIHPveY51cW6pIHHuZYx6e2m\|	NUjtd3hxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4N\|U2OzdpPkK1PFM2PTN5PD;hQi=>
MCF7	MkTWSpVv[3Srb36gZZN{[Xl?		M2Hw[VE3KGi{cx?=	NWToOWlmSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEhfHKjboPm[YN1\WRiaX6gbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiBzNjDodpMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyIE2gNE4xQDdizszNMi=>	M320TlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkK2OFkxLz5{NkKyOlQ6ODxxYU6=
HEK293	MlnvSpVv[3Srb36gZZN{[Xl?	MVexNEB2VQ>?	Moq1NlQhcHK\|	MmG3RYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIF{e2W\|c3XkJIF{KHKnY3XweI9zKHS{YX7zZYN1cX[jdHnvckBifCBzMDD1UUBqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk>	MkTrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ\|OESyPFYoRjJ4M{i0Nlg3RC:jPh?=
3T3L1	M3PGSmZ2dmO2aX;uJIF{e2G7	MYWyJJVO		MlnSWJJidnOjY4TpeoF1cW:wIH;mJHBRSVKpYX3tZUBqdiCvb4Xz[UA{XDOOMTDj[YxteyCjc4Pld5Nm\CCjczDh[Ilxd2O7dHWg[Iln\mW{ZX70bYF1cW:wIHL5JI1m[XO3cnnu[{BtcXCrZDDhZ4N2dXWuYYTpc44h[XRiMjD1UUBjgSCRaXygVoVlKE9ic4ThbY5qdmdvYnHz[YQh[XO\|YYm=	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7NUe0PUc,OjZ3OUW3OFk9N2F-
3T3L1	M172[WZ2dmO2aX;uJIF{e2G7	MYCyJJVO		NGnBbJRVemGwc3HjeIl3[XSrb36gc4YhWFCDUnfhcY1iKGmwIH3veZNmKDOWM1yxJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKE[DQmC0JIV5eHKnc4Ppc44h[XRiMjD1UUBjgSCzUFPSJI1mfGixZB?=	NGO1dpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5OVc1QSd-Mk[1PVU4PDl:L3G+
3T3L1	M{H0TGZ2dmO2aX;uJIF{e2G7	NIXMXZQzKHWP		M3vTbXRz[W6\|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5ibX;1d4UhO1R\|TEGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hT1SXVESg[ZhxemW\|c3nvckBifCB{IIXNJIJ6KHGSQ2KgcYV1cG:m	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7NUe0PUc,OjZ3OUW3OFk9N2F-
3T3L1	NUDkTZk{TnWwY4Tpc44h[XO\|YYm=	MoDNNkB2VQ>?		M2fVTnRz[W6\|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5ibX;1d4UhO1R\|TEGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4h[WSrcH;u[YN1cW5iZYjwdoV{e2mxbjDheEAzKHWPIHL5JJFRS1JibXX0bI9l	M2rNWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUm1O\|Q6Lz5{NkW5OVc1QTxxYU6=
3T3L1	NEC5O5NHfW6ldHnvckBie3OjeR?=	NEDsV4wzKHWP		M2nFRXRz[W6\|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5ibX;1d4UhO1R\|TEGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hVFCOIHX4dJJme3Orb36gZZQhOiC3TTDifUByWEOUIH3leIhw\A>?	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7NUe0PUc,OjZ3OUW3OFk9N2F-
HEK293	MWjGeY5kfGmxbjDhd5NigQ>?	M{nnW\|EhfU1?	MWqyOEBpenN?	NXz2bJdKSWexbnnzeEBi[3Srdnn0fUBifCCSUFHSJIdidW2jIDj1cotvd3ewIH;ybYdqdilidILhcpNn\WO2ZXSgbY4hUEWNMkmzJINmdGy\|IHH0JFEhfU1iYX\0[ZIhOjRiaILzJIJ6KGS3YXytcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6	MljMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ7OEizNFQoRjJ4OUi4N\|A1RC:jPh?=
C2C12	MVPGeY5kfGmxbjDhd5NigQ>?	MlvqNlAhfU1?	NFf4O281KGi{cx?=	M1rkfnRz[W6\|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5ibX;1d4UhSzKFMUKgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hT0yXVD20JI1m\GmjdHXkJFIuVkKGRzD1dJRic2ViYYSgNlAhfU1iYYSgOEBpenNiYomg[ox2d3Knc3PlcoNmKGG\|c3H5	MmHDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzMEC5PVMoRjJ5MUCwPVk{RC:jPh?=
HEK293	MV7GeY5kfGmxbjDhd5NigQ>?		NETqO3IzPCCqcoO=	NV;Hc5drSWexbnnzeEBi[3Srdnn0fUBifCCJQVy0MZRi\2enZDDoeY1idiCSUFHS[4FudWFibHnnZY5lKGKrbnTpcoch\G:vYXnuJINpcW2ncnnjJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvOjZizszNMi=>	M{DSTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NUmxNFA3Lz5{N{W5NVAxPjxxYU6=
HepG2	NH\kTplHfW6ldHnvckBie3OjeR?=		M1ftSVIxKGi{cx?=	NETCVHlVemGwc3HjeIl3[XSrb36gc4YhT0GOND3meZNm\CCqdX3hckBRWEGUZ3HtcYEhVEKGIHX4dJJme3OnZDDpckBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjCyNEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yNDFOwG0v	Mnz5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyN{[4NlcoRjJ6MEe2PFI4RC:jPh?=
HT-29	NFPrO5lHfW6ldHnvckBie3OjeR?=	NYHDTGlyOTBidV2=	MVqyOEBpenN?	M1jGSWFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGmwIHj1cYFvKEiWLUK5JINmdGy\|IHjhdoJwemmwZzDBVGMhdXW2YX70JIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[gW451N2KndHGtZ4F1\W6rbjDzbYdv[WyrbnegdIF1cHejeTDifUBu\WG\|dYLpcoch\GWlcnXhd4UhcW5iQ4njcIlvTDFibHX2[Ywh[XRiMUCgeW0hfHKnYYTl[EBnd3JiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBu\XSqb3S=	MkjhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyN{[4NlcoRjJ6MEe2PFI4RC:jPh?=
COS7	MXHGeY5kfGmxbjDhd5NigQ>?		M{DpWVI1KGi{cx?=	MVjUdoFve2GldHn2ZZRqd25ib3[gbJVu[W5iR3HsOE1nfXOnZDDQVGFT\2GvbXGgUGJFKGW6cILld5Nm\CCrbjDB[pJq[2GwIHfy[YVvKG2xbnvlfUBEV1N5IHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCwOEDPxE1w	NVjIcYhXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2NFk4ODhpPkK4OlA6PzB6PD;hQi=>
COS7	MXnGeY5kfGmxbjDhd5NigQ>?		NHzuT5ozPCCqcoO=	MYjUdoFve2GldHn2ZZRqd25ib3[gbJVu[W5iR3HsOE1nfXOnZDDQVGFT[WyyaHGgUGJFKGW6cILld5Nm\CCrbjDB[pJq[2GwIHfy[YVvKG2xbnvlfUBEV1N5IHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSBzMDFOwG0v	NUHTW2dFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2NFk4ODhpPkK4OlA6PzB6PD;hQi=>
HEK293	MVnGeY5kfGmxbjDhd5NigQ>?		NV\GboFCOThiaILz	MlHKRYdwdmm\|dDDhZ5Rqfmm2eTDheEBISUx2LX\1d4VlKFCSQWLnZY1u[SCOQlSgLJVvc26xd36gc5Jq\2mwKTDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHHzd4V{e2WmIHHzJJJm[2WydH;yJJRz[W6\|YXP0bZZifGmxbjDh[pRmeiBzODDodpMh[nliZIXhcEBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkC0JO69VS5?	NGOyclA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[2O\|g4Pid-Mki2Olc5PzZ:L3G+
Ac2F	NGLUS21HfW6ldHnvckBie3OjeR?=	NIntPGM2KHWP	NYXzSWVyPiCqcoO=	NInBR\|BC\2:waYP0JIFkfGm4aYT5JIF1KG[3bHygcIVv\3SqIHj1cYFvKG[uYXeteIFo\2WmIGDQRXJo[W2vYUGgUGJFKGW6cILld5Nm\CCrbjDyZZQhSWN{RjDj[YxteyClb3X4dJJme3OrbnegVHBTTS2[Mz3UT{BifCB3IIXNJIFnfGW{IE[gbJJ{KGK7IH;u[U1odG9ibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5	M33aO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7M{i5NVIyLz5{OUO4PVEzOTxxYU6=
SK-N-MC	MlT1dWhVWyCjc4PhfS=>			NYf1OpJpeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IGPLMW4uVUNiY3XscJM>	MnXYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
3T3-L1	MUXGeY5kfGmxbjDhd5NigQ>?	MlrlNUB2VQ>?	NXTGWnVkOTViZHH5dy=>	Ml7uRYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhcW5ibX;1d4UhO1R\|LVyxJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKEOGM{[gcXJPSSCneIDy[ZN{cW:wIHH0JFEhfU1iYX\0[ZIhOTViZHH5d{BjgSCzdXHueIl1[XSrdnWgdoVidC22aX3lJHBEWiCvZYToc4Q>	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTh5OEe2O{c,Ojl6N{i3Olc9N2F-
3T3-L1	MljqSpVv[3Srb36gZZN{[Xl?	NEnmbYoyKHWP	NH3wbmQyPSCmYYnz	NUXzOYU2SWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiaX6gcY92e2ViM2SzMWwyKGOnbHzzJIF{e2W\|c3XkJIF{KGmwZIXjeIlwdiCxZjDh[Ilxd2O7dHWg[Iln\mW{ZX70bYF1cW:wIHH0JFEhfU1iYX\0[ZIhOTViZHH5d{BjgSCRaXygVoVlKE9ic4ThbY5qdmdvYnHz[YQh[XO\|YYm=	MmjJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl6N{i3OlcoRjJ7OEe4O\|Y4RC:jPh?=
3T3-L1	NF\VZoZHfW6ldHnvckBie3OjeR?=	NUfnWo5tOSC3TR?=	MVSxOUBl[Xm\|	NVrCWZRDSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiaX6gcY92e2ViM2SzMWwyKGOnbHzzJIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIFPQWFFCKG2UTlGg[ZhxemW\|c3nvckBifCBzIIXNJIFnfGW{IEG1JIRigXNiYomgdZVidnSrdHH0bZZmKHKnYXyteIlu\SCSQ2KgcYV1cG:m	NV\4RoRZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm4O\|g4PjdpPkK5PFc5PzZ5PD;hQi=>
HepG2	NWizWIJMTnWwY4Tpc44h[XO\|YYm=		MWqyNEBpenN?	NYrLUplbXHKjboPhZ5RqfmG2aX;uJI9nKEeDTESteIFo\2WmIHj1cYFvKFCSQWLnZY1u[SCOQlSg[ZhxemW\|c3XkJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFIxKGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCzPUDPxE1w	NWLwXWJ[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CxPVkzPTNpPkOwNVk6OjV\|PD;hQi=>
3T3L1	NF;wXoJHfW6ldHnvckBie3OjeR?=	M4HYTVUhfU1?		M1HPT2lv\HWldHnvckBw\iCuaYDp[EBi[2O3bYXsZZRqd25iaX6gcY92e2ViM2SzUFEh[2WubIOgZZQhPSC3TTDifUBQcWxiUnXkJG8he3SjaX7pcocu[mG\|ZXSgZZN{[Xl?	MkWyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzOUmyOVMoRjNyMUm5NlU{RC:jPh?=
3T3L1	Mn;vSpVv[3Srb36gZZN{[Xl?	M2HWZ\|IhfU1?		MkOwTY5lfWO2aX;uJI9nKGyrcHnkJIFk[3WvdXzheIlwdiCrbjDtc5V{\SB\|VEPMNUBk\WyuczDheEAzKHWPIHL5JG9qdCCUZXSgU{B{fGGrbnnu[{1j[XOnZDDhd5NigQ>?	M361T\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMUm5NlU{Lz5\|MEG5PVI2OzxxYU6=
HepG2	M3zuOmZ2dmO2aX;uJIF{e2G7	NYC5dWZlRjFyMDDuUS=>	NH3nbWgzOCCqcoO=	NUTnbHV1XHKjboPhZ5RqfmG2aX;uJI9nKEeDTESteIFo\2WmIHj1cYFvKFCSQWLnZY1u[SCOQlSg[ZhxemW\|c3XkJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGG2IE6xNFAhdk1iYX\0[ZIhOjBiaILzJIlvKHC{ZYPlcoNmKG:oIEWgeW0hWFCDUnfhcY1iKGGwdHHnc45qe3RiR2e5OlYzKGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=>	MmLQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzOUmyOVMoRjNyMUm5NlU{RC:jPh?=
COS7	MlW1SpVv[3Srb36gZZN{[Xl?		NHnZW5ozPCCqcoO=	M4XiNGFod26rc4SgZYN1cX[rdImgZZQhemWlb33ibY5idnRiaIXtZY4hT0GOND3ERmQh\nW\|ZXSgVHBCWmejbX3hJGxDTCCneIDy[ZN{\WRiaX6gR29UPyClZXzsd{Bi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5\|OU[g{txONg>?	MoDMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{MkC2NFIoRjNyMkKwOlAzRC:jPh?=
COS7	MXHGeY5kfGmxbjDhd5NigQ>?		NYnxSFR7OjRiaILz	NXjjXWFPSWexbnnzeEBi[3Srdnn0fUBifCC{ZXPvcYJqdmGwdDDoeY1idiCJQVy0MWRDTCCodYPl[EBRWEGUZ3HtcYEhVEKGIHX4dJJme3OnZDDpckBEV1N5IHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkO5OkDPxE1w	M1;mWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMkSxPVA4Lz5\|MEK0NVkxPzxxYU6=
HEK293	MX;GeY5kfGmxbjDhd5NigQ>?		NXXaUVBVOThiaILz	M4XKRmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gS2FNPCCodYPl[EBRWEGUZ3HtcYEuVEKGIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYX\0[ZIhOThiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNVIh\|ryPLh?=	NIPmR4M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEK5OlY5PSd-M{CyPVY3QDV:L3G+
HEL 293H	M4n4W2Z2dmO2aX;uJIF{e2G7		MYmxOkBpenN?	NX3ONVBKXHKjboPhZ5RqfmG2aX;uJI9nKEeDTEStSGJFKG[3c3XkJIh2dWGwIGDQRXJo[W2vYTDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIGXBV{1jdGFiSFXMJFI6O0hiY3XscJMheHKnaX7jeYJifGWmIH\vdkAyPiCqcoOg[o9tdG:5ZXSgZpkhTlKHVDDzeYJ{fHKjdHWgZYRlcXSrb36gZY5lKG2nYYP1doVlKGGodHXyJFIhcHK\|IHL5JHRTNU[URWSgZZN{[XluIFXDOVAhRSByLkCwPEDPxE1w	Mn3zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB2MkmwPVcoRjNyNEK5NFk4RC:jPh?=
HEK293	NVTvXVBWTnWwY4Tpc44h[XO\|YYm=		NYP2SHI3OThiaILz	M2\jUXRz[W6\|YXP0bZZifGmxbjDv[kBpfW2jbjDQVGFT\2GvbXGg[ZhxemW\|c3XkJIlvKEiHS{K5N{Bk\WyuczDpcoN2[mG2ZXSg[o9zKDF6IHjyd{BjgSCmdXHsJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMEO5JO69VS5?	MkXSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVIyNyd-Q3jFUWJNRC:jPh?=
CV-1	MXPGeY5kfGmxbjDhd5NigQ>?			M{f2[XRme3SnZDDmc5IhfHKjboPhZ5RqfmG2aX;uJI9nKGi3bXHuJHBRSVJiZ3HtcYEuT2GuNDDjbIlu\XKjIHnuJGNXNTFiY3XscJMtKEWFNUCgQUAxNjB3IN88UU4>	NE\oOHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjFxJ{7DbGVOSkx:L3G+
3T3L1	NXTxPHRiTnWwY4Tpc44h[XO\|YYm=		MXy3JIRigXN?	NXi5S\|RxXXC{ZXf1cIF1cW:wIH;mJGZCWyCycn;0[YlvKGW6cILld5Nqd25iaX6g[Iln\mW{ZX70bYF1\WRibX;1d4UhO1R\|TEGgZ4VtdHNiYX\0[ZIhPyCmYYnzJIJ6KHenc4Tldo4h[myxdDDhcoFtgXOrcx?=	M4HMdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzOS9pPlPoSW1DVDxxYU6=
3T3L1	NIjxXYRHfW6ldHnvckBie3OjeR?=		NUHpPXozPyCmYYnz	M13POHVxemWpdXzheIlwdiCxZjDhVFIheHKxdHXpckBmgHC{ZYPzbY9vKGmwIHTp[oZmemWwdHnheIVlKG2xdYPlJFNVO0xzIHPlcIx{KGGodHXyJFch\GG7czDifUB4\XO2ZYLuJIJtd3RiYX7hcJl{cXN?	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlEwLz6FaFXNRmw9N2F-
3T3L1	NHLtepBHfW6ldHnvckBie3OjeR?=		MnjkNlQhcHK\|	Mlz2TY5pcWKrdHnvckBw\iClaILvcolkKGmwc4XsbY4ue3SrbYXsZZRm\CCrboP1cIlvKHKnc3nzeIFv[2ViaX6g[Iln\mW{ZX70bYF1\WRibX;1d4UhO1R\|TEGgZ4VtdHNiYYPz[ZN{\WRiYYOgNk1l\W:6eT3EMXs{UF1vZ3z1Z49{\SC3cIThb4Uh[W[2ZYKgNlQhcHK\|IHL5JJNkcW62aXzsZZRqd25iY3;1cpRqdmdiYX7hcJl{cXN?	NWriOJBKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJzLze+R4hGVUKOPD;hQi=>
3T3L1	M1vReGZ2dmO2aX;uJIF{e2G7	NWLwN3FLOTBidV2=	MmjHNlQhcHK\|	MkTOTY5lfWO2aX;uJI9nKGmwc4XsbY4ue3SrbYXsZZRm\CB{LXTlc5h6NURvW{PIYU1odHWlb4PlJJVxfGGtZTDpckBlcW[oZYLlcpRq[XSnZDDtc5V{\SB\|VEPMNUBk\WyuczDheEAyOCC3TTDh[pRmeiB{NDDodpMh[nlic3PpcpRqdGyjdHnvckBkd3WwdHnu[{BidmGueYPpdy=>	MkDyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVIyNyd-Q3jFUWJNRC:jPh?=
3T3L1	MVvGeY5kfGmxbjDhd5NigQ>?	Mmq0NkB2VQ>?	MW[xOEBl[Xm\|	NHHNSoxC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWKgbY4hdW:3c3WgN3Q{VDFiY3XscJMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gZYRqeG:leYTlJIRq\m[ncnXueIlifGmxbjDheEAzKHWPIHHmeIVzKDF2IHThfZMh[nliT3nsJHJm\CCRIIP0ZYlvcW6pIH3leIhw\A>?	MlnlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVIyNyd-Q3jFUWJNRC:jPh?=
U2OS	M{jpZmZ2dmO2aX;uJIF{e2G7	NFPwRnUyOCC3TR?=	NHHz[YwzPCCqcoO=	NYD3UlFQSWO2aY\heIlwdiCxZjDQVGFTTyBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDoeY1idiCXMl;TJINmdGy\|IHH0JFExKHWPIHnuZ5Vj[XSnZDDmc5IhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeR?=	NFWzZXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjFxJ{7DbGVOSkx:L3G+

他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

アッセイ

Methods	Test Index	PMID

Western blot	Cyclin D1 / CDK2 / CDK4 / Bax / Bcl-2	29725405
Immunofluorescence	PPARγ / E-cadherin	27145370
Growth inhibition assay	Cell viability	29725405

体内試験

Rosiglitazone administration results in significant bone loss, including a decrease in bone volume, trabecular width, and trabecular number and an increase in trabecular spacing. Rosiglitazone also leads to a decrease in bone formation rate, with a simultaneous increase in fat content in the bone marrow. Rosiglitazone decreases the expression of the osteoblast-specific genes Runx2/Cbfa1, Dlx5, and alpha1(I)collagen, whereas the expression of the adipocyte-specific fatty acid binding protein aP2 is increased. ^[2] Rosiglitazone upregulates gene transcripts encoding mitochondrial proteins in white adipocytes from ob/ob mice accompanied by an increase in mitochondrial mass and changes in mitochondrial structure. ^[4]

お薦めの試験操作（参考用のみ）

参考	[1] Fryer LG, et al. J Biol Chem, 2002, 277(28), 25226-25232. [2] Rzonca SO, et al. Endocrinology, 2004, 45(1), 401-406. [3] Motoshima H, et al. J Clin Endocrinol Metab, 2002, 87(12), 5662-5667. [4] Wilson-Fritch L, et al. J Clin Invest, 2004, 114(9), 1281-1289. [5] Yasser Majeed, et al. Mol Pharmacol . 2011 Jun;79(6):1023-30. [6] Yang Li, et al. Cell Death Differ . 2019 Nov;26(11):2284-2299. + 展開

参考

+ 展開

溶解度 (25°C)

体外


体内	左から（NMPから）右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ): 4% DMSO+30% PEG 300+5% Tween 80+ddH2O 混合させたのち直ちに使用することを推奨します。注文はこちらから: PEG300 Tween 80	5mg/mL

化学情報

分子量	357.43
化学式	C₁₈H₁₉N₃O₃S
CAS No.	122320-73-4
Storage	3年	-20°C	粉
Storage	2年	-80°C	in solvent
Smiles	CN(CCOC1=CC=C(C=C1)CC2C(=O)NC(=O)S2)C3=CC=CC=N3

Rosiglitazone (BRL 49653) SDFをダウンロードする

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

便利ツール

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

臨床試験

NCT Number	Recruitment	Interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT02737709	Terminated	Drug: Paclitaxel and Carboplatin regimen	Non-small Cell Lung Cancer	Sun Yat-sen University	March 2016	Phase 2
NCT01402076	Completed	Drug: Tasimelteon\|Drug: Rosiglitazone\|Drug: Midazolam	Healthy Volunteers	Vanda Pharmaceuticals	August 2011	Phase 1
NCT01100619	Completed	Drug: rosiglitazone\|Drug: XL184	Papillary Thyroid Cancer\|Follicular Thyroid Cancer\|Huerthle Cell Thyroid Cancer\|Renal Cell Carcinoma	Exelixis	April 2010	Phase 1
NCT01415128	Completed	Drug: Omeprazole\|Drug: Rosiglitazone\|Drug: Desipramine	Erectile Dysfunction	VIVUS LLC	April 2010	Phase 1
NCT01376063	Completed	Drug: FG-4592	Healthy Adult Subjects	FibroGen	March 2010	Phase 1

(data from https://clinicaltrials.gov, updated on 2022-08-01)

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

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電話番号

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