3-Methyladenine (3-MA)

製品コード:S2767 別名:NSC 66389

For research use only.

3-Methyladenine (3-MA, NSC 66389) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation. 3-Methyladenine (3-MA) is successfully used to suppress mitophagy. Solutions of 3-MA are best fresh-prepared by heating.

3-Methyladenine (3-MA)化学構造

CAS No. 5142-23-4

サイズ 価格(税別)
JPY 13600
JPY 63400

代表番号: 03-5615-9297|電子メール:[email protected]
よく尋ねられる質問

文献中Selleckの製品使用例(705)

製品安全説明書

PI3K阻害剤の選択性比較

相関PI3K製品

生物活性

製品説明 3-Methyladenine (3-MA, NSC 66389) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation. 3-Methyladenine (3-MA) is successfully used to suppress mitophagy. Solutions of 3-MA are best fresh-prepared by heating.
ターゲット
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
体外試験

The slight preference for Vps34 prevention by 3-Methyladenine probably arises from a hydrophobic ring specific to Vps34, which encircles the 3-methyl group of 3-Methyladenine. [1] 3-Methyladenine has been reported to cause cancer cell death under both normal and starvation conditions. 3-Methyladenine could also suppress cell migration and invasion independently of its ability to inhibit autophagy, implying that 3-Methyladenine possesses functions other than autophagy suppression. 3-Methyladenine elicits caspase-dependent cell death that is independent of autophagy inhibition. Treatment with 5 mM 3-Methyladenine reduces the percentage of glucose-starved HeLa cells displaying GFP-LC3 puncta to 23%. The levels of LC3-I are increasing and the levels of LC3-II are decreasing between 12 and 48 hours in cells that are treated with 3-Methyladenine. Conversion of LC3-I to LC3-II is suppressed by 3-Methyladenine. Treatment of HeLa cells with 3-Methyladenine at 2.5 mM or 5 mM for one day does not affect cell viability, whereas treatment with 10 mM 3-Methyladenine for one day causes a 25.0% decrease in cell viability. Treatment of cells with 2.5, 5 or 10 mM 3-Methyladenine for two days causes 11.5%, 38.0% and 79.4% decrease in viability, respectively. 3-Methyladenine decreases cell viability in a time- and dose-dependent manner. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. [2] Suppression of autophagy by 3-Methyladenine inhibits SU11274-induced cell death. [3] Prolonged treatment with 3-Methyladenine (up to 9 hours) induces significant LC3 I to II conversion in wild type MEFs. Prolonged treatment with 3-Methyladenine, but not wortmannin, markedly increases GFP-LC3 punctuation/aggregation. 3-Methyladenine-induced LC3 conversion and free GFP liberation are ATG7-dependent. 3-Methyladenine treatment leads to evident increase of p62 protein level. 3-Methyladenine increases the p62 level even in Atg5−/− MEFs as well as in cells with DOX-mediated deletion of ATG5. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine-induced LC3 I to LC3 II conversion is dramatically compromised in Tsc2−/− cells compared with wild type cells.3-Methyladenine disrupts the anti-autophagic function of mTOR complex 1. [4]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K562 MXvGeY5kfGmxbjDBd5NigQ>? NEPifZIyOG2P NGTvZZkycA>? Ml;w[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIH;mJGxEOy2LSTDhcoQhfGinIH\vdo1ifGmxbjDv[kBifXSxcHjh[49{d22ncx?= NVS4UGE3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4OlQxOzdpPkKxPFY1ODN5PD;hQi=>
Jurkat MUnGeY5kfGmxbjDBd5NigQ>? MoTDNVBuVQ>? MnLhNYg> Mon2[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIH;mJGxEOy2LSTDhcoQhfGinIH\vdo1ifGmxbjDv[kBifXSxcHjh[49{d22ncx?= NX7n[nBHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4OlQxOzdpPkKxPFY1ODN5PD;hQi=>
HeLa  NHrGc5NEgXSxdH;4bYNqfHliQYPzZZk> MYKycW0> MV6yOIg> NWHsVXNrcW6qaXLpeIV{KHSqZTDjfZRwfG:6aXPpeJkhd2Zic3nsbYJqdmmwIITvJGhmVGFiY3XscJMv NESx[FI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi3OVM5PSd-MkG4O|U{QDV:L3G+
PC12/TetOn M2[wcmZ2dmO2aX;uJGF{e2G7 NUjZXJM4OC5zL{HtUS=> MkP3NVhp NXfmOI5{dGWjZIOgeI8h|rFvc4nuLHdVMSCjY3P1cZVt[XSrb36sJJRwgGmlaYT5MEBidmRib3zp[49u\XJiZn;ycYF1cW:wwrC= MmXQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7ME[2OVkoRjJzOUC2OlU6RC:jPh?=
RMPI8226  MWPGeY5kfGmxbjDBd5NigQ>? NYC3eXZbPW2P MkKxNYg> MmfHd5VxeHKnc4Pld{B1cGVibHX2[Ywhd2ZiYYX0c5Bp[We7IIXu[IVzKG63dILp[Y51KGSncHzleIlwdg>? MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTlzNU[yNEc,OjF7MUW2NlA9N2F-
MCF-7 NIjofo9HfW6ldHnvckBCe3OjeR?= NHvSZ28yOG2P MWW0PIg> MUDicI9kc3NiYYX0c5Bp[We7IHnu[JVk\WRiYomgZo9zfGW8b33pZi=> NF7nNXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUmzNVk{Pyd-MkG5N|E6Ozd:L3G+
HBx  NIDVW|RCeG:ydH;zbZMhSXO|YYm= MWWxNI1O NYXiOpFiPDiq Mn:wSG1UVw>? NYjtd2J1cW6lcnXhd4V{KGOnbHyg[IVifGh? MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB{MEC3PEc,OjJyMkCwO|g9N2F-
Marc-145 MmLPSpVv[3Srb36gRZN{[Xl? Mk\NOY1O NVT1OYpQOTJxMkSvN|Zp NV;yS2J4emWmdXPld{B1cGViUGLSV3YhfGm2ZYLzJIFv\CC2aHWgdJJwfGWrbjDlfJBz\XO|aX;u NUHzTFdGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNVk6ODBpPkKyNVE6QTByPD;hQi=>
U937 NVv1flUxTnWwY4Tpc44hSXO|YYm= MV:ycW0> MXmxNog> MUPk[YNz\WG|ZYOgeIhmKGG3dH;wbIFogSC{YYTpc:Kh MnPvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzNUWxOVAoRjJ{MUW1NVUxRC:jPh?=
BGC-823 NF\WO3lHfW6ldHnvckBCe3OjeR?= MlO4OY1O MonBNog> NHr5[XpqdmirYnn0d{B1cGVicnH0[UBw\iCjdYTvdIhi\2mlIHPlcIx{ NGruN4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkOyNlE2Oid-MkKzNlIyPTJ:L3G+
A549 NVzsbZNFTnWwY4Tpc44hSXO|YYm= NIrtWmkxNjGvTR?= NGrFOogzPGh? MYrzeZBxemW|c3XzJHNWOTF{N{StbY5lfWOnZDDj[YxtKGSnYYTo NVX1[Xc2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0OlY6PjBpPkKyOFY3QTZyPD;hQi=>
pDCs M4P4OmZ2dmO2aX;uJGF{e2G7 MknJNVBuVQ>? Mn3yNE42cA>? MlrKxsBz\WS3Y3XzJJRp\SCrbnT1Z5Rqd25ib3[gTWZPNc7zIHL5JJN{Wk6DNEC= NEXRdnY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkO5OlU6QSd-MkKzPVY2QTl:L3G+
HeLa  NUfKZmdKSXCxcITvd4l{KEG|c3H5 MnvhOY1O MU[yOIg> NUTxcmd{cW6mdXPld{Bk[XOyYYPlMYRmeGWwZHXueEBk\WyuIHTlZZRp NULQNmpVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1OFUyOjhpPkKyOVQ2OTJ6PD;hQi=>
U251  NHW5[IpCeG:ydH;zbZMhSXO|YYm= NWO5U3BYPW2P NWfuOHRNOjSq NHf4NYhqdmO{ZXHz[ZMhWzFvaX7keYNm\CClZXzsJIRm[XSq MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV5OUe4PEc,OjJ3N{m3PFg9N2F-
MCF-7  MXjBdI9xfG:|aYOgRZN{[Xl? MkLqNE4ydU1? NYTSRWZxPmh? MULlcohidmOnczDzbZJ1cW6xbD3pcoR2[2WmIHHwc5B1d3Orcx?= MkDiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NUG5PFkoRjJ{N{WxPVg6RC:jPh?=
PC-3  M{DTTGFxd3C2b4Ppd{BCe3OjeR?= MlLINo1O MUiybC=> MXTpcoNz\WG|ZYOgU3JKNWmwZIXj[YQh[2WubDDk[YF1cA>? MkTKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEW1PFAoRjJ{N{S1OVgxRC:jPh?=
HCT116 Ml\KRZBweHSxc3nzJGF{e2G7 NX;uZWk4PW2P M3jNcFI1cA>? M1PSZmROW09? NIDnT3NmdmijbnPld{BieGmpZX7pck1qdmS3Y3XkJINmdGxiZHXheIg> NGXWbpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[yOlUzOid-MkS2NlY2OjJ:L3G+
U2OS MmS4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFPPVGoyOG2P MWiyOIg> MYrpcpRmdnOrZnnld{B1cGViZ4Lve5RpKGmwaHnibZRqd25iaX7keYNm\CCkeTDEc5g> NFjnU|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[zPVAyOyd-MkS2N|kxOTN:L3G+
A2780cp M4HmSmFxd3C2b4Ppd{BCe3OjeR?= NUHQUFBWOi53bV2= MoG1NYg> NWnoUWh3\GSKMl:= MoP3[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NYH1cpBjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVc6PDZpPkK0PFE4QTR4PD;hQi=>
HepG2 NWHNVnI5TnWwY4Tpc44hSXO|YYm= M3jFOlVuVQ>? MmrPOIg> MYHpcoNz\WG|ZYOgZ4VtdHWuYYKgcIV3\Wy|IH;mJGhNyqB? MmLVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5MUO1PFcoRjJ2N{GzOVg4RC:jPh?=
Microglia  NVHTW|VwSXCxcITvd4l{KEG|c3H5 NGfZNok2dU1? M1jlOFI1cA>? NYDWfpRw\GWlcnXhd4V{KGi7cH;4bYEucW6mdXPl[EBk\WyuIHTlZZRp M2HFTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEG4OlAyLz5{NEixPFYxOTxxYU6=
MDA-MB 231 NVHRUoRbSXCxcITvd4l{KEG|c3H5 M4jLV|VuVQ>? M2TUOlAvPWh? MnHTcY9lfWyjdHXzJHRw[2:vaX9CsuKhcW6mdXPl[EBieG:ydH;zbZM> NH:xdYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEizNFc5OSd-MkS4N|A4QDF:L3G+
PANC-1  NWjsRnhnSXCxcITvd4l{KEG|c3H5 MYKxcW0> MX:0PIg> MUnEUXNQ NHS2ZlFmdmijbnPld{Bjd3K2ZYrvcYljNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? NGPvPYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi0NlE2QCd-MkS4OFIyPTh:L3G+
MDA-MB-231 NXXIO|dqTnWwY4Tpc44hSXO|YYm= NXv4OpNXOm2P MUm0PIg> MXjwdo9ud3SnczDUUU1qdmS3Y3XkJINmdGxiZHXheIg> NWXYUGpyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5O|A3PzZpPkK0PVcxPjd4PD;hQi=>
MDA-MB-231 M{\kTmZ2dmO2aX;uJGF{e2G7 M2TZPVJuVQ>? NXzFZlZxOjSq NHTyZo1qdmirYnn0d{BifXSxcHjh[5khcW6mdXPl[EBjgSCWTdMg M1PBc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUewOlc3Lz5{NEm3NFY4PjxxYU6=
MCF-7  NYG5XHN5TnWwY4Tpc44hSXO|YYm= NFvGPVQzdU1? NYrJcIM5PDiq M1rtNpBzd22xdHXzJHRONWmwZIXj[YQh[2WubDDk[YF1cA>? M3y0e|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUewOlc3Lz5{NEm3NFY4PjxxYU6=
MCF-7  MlnqSpVv[3Srb36gRZN{[Xl? NIHXRWEzdU1? M3vWVVI1cA>? NFLkN2tqdmirYnn0d{BifXSxcHjh[5khcW6mdXPl[EBjgSCWTdMg Ml\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7N{C2O|YoRjJ2OUewOlc3RC:jPh?=
HepG2 NF3GcGlCeG:ydH;zbZMhSXO|YYm= NIe0e4c{dU1? M1nE[FVp MUny[YR2[2W|IHPlcIwh[XCxcITvd4l{KGmwZIXj[YQh[nliUVTz M17BSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEO2OVk2Lz5{MkizOlU6PTxxYU6=
HeLa MXLBdI9xfG:|aYOgRZN{[Xl? NF6zT28yOG2P MUWybC=> NF\sNVBl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgZ48ufHKnYYTt[Y51KHerdHigVGVK NVjvSGxHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNFAyOzVpPkKzNFAxOTN3PD;hQi=>
SK-HEP-1 Mn3YRZBweHSxc3nzJGF{e2G7 NVXv[|ZSOTCvTR?= MYKxbC=> M1Kzd5Bzd3SnY4TzJIFo[Wmwc4SgZZV1d3CqYXf5JIFv\CCrbnT1Z4V{KGGyb4D0c5NqeyCrbjDieYZidGmwLYTy[YF1\WRiY3XscJM> MkHlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6NUi2OFkoRjJ{OEW4OlQ6RC:jPh?=
MDA-MB231 MX7GeY5kfGmxbjDBd5NigQ>? NIe2e2Q2dU1? NX:2S3R{OWh? MnfKbY5kemWjc3XzJJJme3[ncnH0do9tNW2nZHnheIVlKGOjc4Dhd4Uh[WO2aY\heIlwdiCjbnSgZ4VtdCCmZXH0bC=> MkXQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyOEi4OVAoRjJ|MEi4PFUxRC:jPh?=
PaCa44 MmnFRZBweHSxc3nzJGF{e2G7 Mk\GNk42dU1? Mn\ONYg> MlK3doVlfWOnc9Mg[4VvcXCrbj3t[YRq[XSnZDDhdI9xfG:|aYO= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF{NEGxNkc,OjNzMkSxNVI9N2F-
T-47D NXTyWFZyTnWwY4Tpc44hSXO|YYm= M3nteVExdU1? Moq1Nog> M4LwVYlvcGmkaYTzJIF2fG:yaHHnfUBxem:lZYPzJIFv\CCrbnPy[YF{\XNicnHwZY16[2mwIHnu[JVk\WRiYYDvdJRwe2m| NULqOIZCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzNFAxOjZpPkKzN|AxODJ4PD;hQi=>
GTL-16  NIrLeI9CeG:ydH;zbZMhSXO|YYm= NEHifJg2dU1? NYrXR4FqOjSq NFv3Uo9z\WS3Y3XzJINmdGxidnnhZoltcXS7IHHzJINwdXCjcnXkJJRwKGOnbHzzJJRz\WG2ZXSge4l1cCCPRWSgbY5pcWKrdH;ydy=> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzNzM{S5NEc,OjN|MUO0PVA9N2F-
U251MG  MmPvSpVv[3Srb36gRZN{[Xl? MkLxN41O MWmxbC=> M3jpTpN2eHC{ZYPz[ZMhVEN|LVnJJJBzd3SnaX6g[ZhxemW|c3nvci=> NYjtZ|Q3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzN|g3OThpPkKzN|M5PjF6PD;hQi=>
T24 M36zb2Z2dmO2aX;uJGF{e2G7 NXrZe|czOTCvTR?= MUSxbC=> NGHqOYxz\WS3Y3XzJJRp\SClbHXheoFo\SCxZjDMR|Mh[W[2ZYKgZoFq[2GuaX6geJJm[XSvZX70 NYTCbmV{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzOVQxQDBpPkKzN|U1ODhyPD;hQi=>
HUVECs M4LWNWZ2dmO2aX;uJGF{e2G7 NF[3VoE{dU1? NHTEOZEzPGh? Mk\DZoxw[2u|IITo[UBxem:2ZXP0bZZmKGWoZnXjeEBw\iC{ZYP2[ZJifHKxbDDifUBqdmirYnn0bY5oKGG3dH;wbIFogQ>? NFfoNJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O1PFkzQCd-MkOzOVg6Ojh:L3G+
MCF-7  NWH0boZGTnWwY4Tpc44hSXO|YYm= M4DG[|VuVQ>? MYiyOIg> MVnpcohq[mm2czDzeIFzfmG2aX;uMYlv\HWlZXSgZZV1d3CqYXf5 Mlm0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|OUW2O|koRjJ|M{m1Olc6RC:jPh?=
Hela  MYLGeY5kfGmxbjDBd5NigQ>? MXO1cW0> NFLaUFIzPGh? NHnjb5BqdmirYnn0d{B{fGG{dnH0bY9vNWmwZIXj[YQh[XW2b4DoZYd6 MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN7NU[3PUc,OjN|OUW2O|k9N2F-
OR6 NGmyRohHfW6ldHnvckBCe3OjeR?= NW\Kb2lTOTCvTR?= NHryUlM4Omh? NX;qOWJpe3WycILld5NmeyCKQ2[gdoVxdGmlYYTpc44h[W6mIH\vdo1ifGmxbjDv[kBifXSxcHjh[49{d22ncx?= NFfqWos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O5OVg4PSd-MkOzPVU5PzV:L3G+
HT-29  M{XCXGZ2dmO2aX;uJGF{e2G7 Mo[xNY1O MVO0PE86Pmh? M4j2RolvcGmkaYTzJGFOWEtiaX7keYNmeyCjdYTvdIhi\2mlIHPlcIwh\GWjdHi= MmnKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3MEiyO|IoRjJ|NUC4NlczRC:jPh?=
SH-SY5Y NGOyNWREgXSxdH;4bYNqfHliQYPzZZk> MUW1cW0> NXO1U4ZtOjSq MVvpcoNz\WG|ZYOgVGNPKHSxeHnjbZR6 NU\teIlQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NlUzPjVpPkKzOVI2OjZ3PD;hQi=>
Saos-2  MUXBdI9xfG:|aYOgRZN{[Xl? M{LjR|FuVQ>? MnzNPVZp NXu1cJFicW6lcnXhd4V{KGOnbHyg[IVifGhiaX7keYNm\CCkeTDQR3g> M3OwOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NU[zNVcyLz5{M{W2N|E4OTxxYU6=
1321N1 NGS1ZoNEgXSxdH;4bYNqfHliQYPzZZk> Ml7SOY1O MkHUNlRp NV;uU5pqeHKxdHXjeJMh[2WubDDh[4FqdnO2IGDDUk1qdmS3Y3XkJJRwgGmlaYT5 M1fHSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUK1NlY2Lz5{M{WyOVI3PTxxYU6=
A2780 NHXHUoxCeG:ydH;zbZMhSXO|YYm= NXmwdYdYPW2P Ml3INlRp MoHzZ49vfmW{dIOgSnR[PzJyIIfpeIghS0SGUDDpcpRwKGGwIHHk[Il1cX[nIHXm[oVkfCC2b4fhdoR{KGurbHzpcochd3[jcnnhckBk[W6lZYKgZ4VtdHN? M4rzbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUmyNlgyLz5{M{W5NlI5OTxxYU6=
OV2008 MnvGRZBweHSxc3nzJGF{e2G7 M37kbVVuVQ>? MoXNNlRp NHzaT2Rkd264ZYL0d{BHXFl5MkCge4l1cCCFRFTQJIlvfG9iYX6gZYRlcXSrdnWg[YZn\WO2IITve4Fz\HNia3nscIlv\yCxdnHybYFvKGOjbnPldkBk\Wyucx?= NHPmRnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W5NlI5OSd-MkO1PVIzQDF:L3G+
PC12  NYfySG9ITnWwY4Tpc44hSXO|YYm= M1vn[|ExdU1? MV:yOIg> NWD4XFBCf2G2ZYK= NUjjPJRxyqCrbnjpZol1eyClaIntc5RzgXC|aX6tcIls\SCycn;0[YF{d22jbDDhZ5Rqfmm2eT6= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZyM{m3PUc,OjN4MEO5O|k9N2F-
SH-SY5Y MXLBdI9xfG:|aYOgRZN{[Xl? NF3PbYk2dU1? MoKyNYg> MXThZo9tcXOqZYOgZ4Vt[XO2cn;sJI5mfXKxcILveIVkfGm4ZTDl[oZm[3R? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZzOUO5OUc,OjN4MUmzPVU9N2F-
SH-SY5Y Mk\2SpVv[3Srb36gRZN{[Xl? M4L2NlFuVQ>? NHHqe5AzPGh? NFjDXVhqdmirYnn0d{B1cGViYYX0c5Bp[We7IHnu[JVk\WRiYomgWG9EWA>? MkHKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5NEOxOFgoRjJ|N{SzNVQ5RC:jPh?=
HepG2 M1zHeWZ2dmO2aX;uJGF{e2G7 MnvnNVBuVQ>? Mn24NlRp NHvFeVFqdmirYnn0d{B{cVSLR1HSMUBidmRiSFLTV{1qdmS3Y3XkJIF2fG:yaHHnfS=> MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzhzN{C0NEc,OjN6MUewOFA9N2F-
HeLa M2i1UmZ2dmO2aX;uJGF{e2G7 NYnmXIk2OTCvTR?= NGHQWW4zcA>? MlG5d5VxeHKnc4Pld{BNSzNiSVmg[ZhxemW|c3nzc44> Ml\4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NkS3N|goRjJ|OE[0O|M5RC:jPh?=
HONE-1  MU\GeY5kfGmxbjDBd5NigQ>? MV:1cW0> MUOxbC=> M2jsT5JmeHKnc4Pld{A3ei2vZXTpZZRm\CCUT2OgdJJw\HWldHnvci=> NHHJ[Zo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i5NlM2QCd-MkO4PVI{PTh:L3G+
MCF7 NEK3TJZHfW6ldHnvckBCe3OjeR?= NFjXb4k2dU1? M2m2VVI1cA>? MlvHbY5kemWjc3XzJGN2VyCrbnT1Z4VlKGOnbHyg[IVifGh? NXzEbI9rRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5OlI3OjlpPkKzPVYzPjJ7PD;hQi=>
HO8910 MWnBdI9xfG:|aYOgRZN{[Xl? MX[xNI1O NFPPcGgyOmh? MYPlcohidmOnczDCNVkucW6mdXPl[EBieG:ydH;zbS=> M2nkfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUizOlExLz5{M{m4N|YyODxxYU6=
SMMC-7721 NHT0eIdCeG:ydH;zbZMhSXO|YYm= NIm1[Gk2dU1? M3m5eVI1cA>? NITuNJdifHSnboXheIV{KFSQRj5OtUBxem:2ZXP0bY9vKGGpYXnud5Qhe2W{dX2gd5Rien[jdHnvck1u\WSrYYTl[EBieG:ydH;zbZM> Mk\2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyNk[2PVMoRjJ2ME[2Olk{RC:jPh?=
Hep3B NXzUcpI6SXCxcITvd4l{KEG|c3H5 MYe1cW0> NHfhbogzPGh? NX\Zdm9b[XS2ZX71ZZRmeyCWTl[t{tEheHKxdHXjeIlwdiCjZ3HpcpN1KHOncoXtJJN1[XK4YYTpc44udWWmaXH0[YQh[XCxcITvd4l{ M2O5cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2ME[2Olk{Lz5{NEC2OlY6OzxxYU6=
H460 MmKxSpVv[3Srb36gRZN{[Xl? NIDNWJYyOG2P MV[0bC=> MWPpcoNz\WG|ZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NGTBd489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG3N|IxQCd-MkSxO|MzODh:L3G+
A549 M3;rbGZ2dmO2aX;uJGF{e2G7 M2Hnc|ExdU1? MnX3OIg> MofSbY5pcWKrdIOgZZV1d3CqYXf5JIlv\HWlZXSgZpkhcXK{YXTpZZRqd25? NYXlW|RuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOFI4OzVpPkK0NVQzPzN3PD;hQi=>
H1299  MoLXSpVv[3Srb36gRZN{[Xl? NXzOb2JpOTCvTR?= NU\YWlA5PGh? NWDVXmJTcW6lcnXhd4V{KGOrc4DsZZRqdi2rbnT1Z4VlKGOnbHyg[IVifGh? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF5M{KwPEc,OjRzN{OyNFg9N2F-
WiDr MmfiSpVv[3Srb36gRZN{[Xl? MkfONVBuVQ>? NXTPdYUyOWh? MoDVbY5pcWKrdIOgVGNDVC2rbnT1Z4VlKEyFMzDJTUBmgHC{ZYPzbY9v MoDKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzOUC0PFkoRjJ2MUmwOFg6RC:jPh?=
LoVo  MXjBdI9xfG:|aYOgRZN{[Xl? MY[1cW0> NXyzZpdsPDiq MnXN[Y5p[W6lZYOgSGNCNWmwZIXj[YQh[XCxcITvd4l{Ng>? NGjlcoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKwNVgyOid-MkSyNFE5OTJ:L3G+
HepG2 E47 MmPkSpVv[3Srb36gRZN{[Xl? NHG1cGszNjWvTR?= NGrTUIQ1QGh? MWHpcoNz\WG|ZYOgeIhmKHSxeHnjbZR6KG:oIFHBMEBDW09uIHHu[EBES2x2 NX3lWmhlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyO|M4OzhpPkK0Nlc{PzN6PD;hQi=>
RKO MWnGeY5kfGmxbjDBd5NigQ>? NHLMfmQzdU1? M4rWOlFp M{HucmROW09? MX;lcohidmOnczDj[YxtKGSnYYToJIJ6KGenbHThcoFugWOrbh?= NXnGVY5MRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPVE4PzdpPkK0NlkyPzd5PD;hQi=>
Hep3B NFHzS45CeG:ydH;zbZMhSXO|YYm= NFTieG0zdU1? NGLRSWwyOmh? M2rUWmROW09? MVHpcohq[mm2czDBXmQ5ODV3LXnu[JVk\WRiY3XscEBl\WG2aB?= NYG0[G9JRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPVc{ODBpPkK0Nlk4OzByPD;hQi=>
ACHN-5968 NGLa[2lCeG:ydH;zbZMhSXO|YYm= NFPzcJg2dU1? M2rEe|Np NIjGNGVmdmijbnPld{Bx[WOuaYThfIVtNW2nZHnheIVlKGGyb4D0c5Nqe8Li NIXCZ249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOwOVYxPCd-MkSzNFU3ODR:L3G+
Huh7 Mne4RZBweHSxc3nzJGF{e2G7 NVHIZ3hwOm2P NF6zO2MyOmh? MnPSSG1UVw>? MknqbY5pcWKrdIOgRXpFQDB3NT3pcoR2[2WmIHPlcIwh\GWjdHi= MmjMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{OUezNFAoRjJ2Mkm3N|AxRC:jPh?=
UOK257 M4Dk[mFxd3C2b4Ppd{BCe3OjeR?= NFyyVJc2dU1? M3\GcVNp Mo\z[Y5p[W6lZYOgdIFkdGm2YYjlcE1u\WSrYYTl[EBieG:ydH;zbZPDqA>? NGfWeHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOwOVYxPCd-MkSzNFU3ODR:L3G+
ECSCs  M2PZTWFxd3C2b4Ppd{BCe3OjeR?= M3rXZVExdU1? M3q0S|Rp MonC[IVkemWjc3XzJJJieGGveXPpck11emWjdHXkJIFxd3C2b4Ppdy=> MmrpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MUmxNFkoRjJ2M{G5NVA6RC:jPh?=
MCF-7  NUO5TFN[TnWwY4Tpc44hSXO|YYm= NYnW[WZbOTCvTR?= M125blI1cA>? NITjVGlqdmirYnn0d{B1cGViYYX0c5Bp[We7IHnu[JVk\WRiYomgZ4hmdW:2aHXyZZB6KGS{dXfz MkXxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MUW1O|goRjJ2M{G1OVc5RC:jPh?=
SGC-7901  MXXBdI9xfG:|aYOgRZN{[Xl? NInxdJMzdU1? M2rHfFFp MoDVbY5kemWjc3XzJGNCNTRiaX7keYNm\CCjcH;weI9{cXN? NWnadHpZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNlE{PDBpPkK0N|IyOzRyPD;hQi=>
SMMC-7721 NVO2NVlNSXCxcITvd4l{KEG|c3H5 MnvBNo1O MVKxbC=> MX7pcoNz\WG|ZYOgR2EuPCCrbnT1Z4VlKGGyb4D0c5Nqew>? NVm5fIZoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNlE{PDBpPkK0N|IyOzRyPD;hQi=>
T24 NV7FSmhzSXCxcITvd4l{KEG|c3H5 NGfQ[2U2dU1? MkThNU42cA>? MkLSdI91\W62aXH0[ZMh[2WuZXPvfIljNWmwZIXj[YQh[XCxcITvd4l{ NIWwPGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO0PVE4Pid-MkSzOFkyPzZ:L3G+
NTUB1 M323W2Fxd3C2b4Ppd{BCe3OjeR?= MWK1cW0> NUi0WmxuOS53aB?= NGGxOZZxd3SnboTpZZRmeyClZXzlZ495cWJvaX7keYNm\CCjcH;weI9{cXN? MkLzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NEmxO|YoRjJ2M{S5NVc3RC:jPh?=
MG-63 NH\sXVhCeG:ydH;zbZMhSXO|YYm= NWCzTJgxOTCvTR?= M3WyUVEzcA>? MXXlcohidmOnczDEVE1qdmS3Y3XkJIFxd3C2b4Ppdy=> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN3OEOwNUc,OjR|NUizNFE9N2F-
MG-63 Mn:0RZBweHSxc3nzJGF{e2G7 M4rI[lAvPS9zbV2= MlvtN|Jp M2K1d4VvcGGwY3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDDhdI9xfG:|aYO= M3Tnd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{W4N|QzLz5{NEO1PFM1OjxxYU6=
MG-63 NUO3N|VrTnWwY4Tpc44hSXO|YYm= MnTLNE42NzGvTR?= NICyeY01QGh? NWixc4Y4cW6mdXPld{B{[Wyrbn;tfYNqdi2rbnT1Z4VlKGOnbHygeoli[mmuaYT5JIxwe3N? Mm\uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NUizOFIoRjJ2M{W4N|QzRC:jPh?=
U2OS  NFzMO4tHfW6ldHnvckBCe3OjeR?= MVywMlUwOW2P MX:0PIg> Mn32bY5lfWOnczDzZYxqdm:veXPpck1qdmS3Y3XkJINmdGxidnnhZoltcXS7IHzvd5M> NHPSRXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO1PFM1Oid-MkSzOVg{PDJ:L3G+
HGC-27 MUHGeY5kfGmxbjDBd5NigQ>? NGO5Z3AyOG2P MkjsNYg> M3vMUYlvcGmkaYTzJJRp\SClZXzsJJZq[WKrbHn0fUBtd3O|IHL5JHJCTDByMTDvdkBOUy1{MkC2 MljIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2MU[zOFkoRjJ2NEG2N|Q6RC:jPh?=
HCT116  M2nhb2Fxd3C2b4Ppd{BCe3OjeR?= NXvwXHV1PW2P M{HzOVI1cA>? NYrXU2Rx\W6qYX7j[ZMhfGinIHHwc5B1d3OrczDpcoR2[2WmIHL5JIFxcWenbnnu M2rZWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkK2OVIzLz5{NE[yOlUzOjxxYU6=
A549 NXXS[XM6SXCxcITvd4l{KEG|c3H5 MWexNI1O MVy0PIg> M1jiZYFk[2WuZYLheIV{KHSqZTDy[YR2[3Srb36gc4Yh[2WubDD2bYFjcWyrdImgbY5lfWOnZDDifUBRXFh? NGjLUW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[yOlczOid-MkS2NlY4OjJ:L3G+
Saos-2 NIHyWY9CeG:ydH;zbZMhSXO|YYm= NXTBWZdNOTCvTR?= NIHQTHgzPGh? NGe1cGNqdnSnboPp[olmeyC2aHWg[5Jwf3SqIHnubIljcXSrb36gc4YhfGinIGWyU3Mh[2WubIOgbY5lfWOnZDDifUBFd3h? MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ|OUCxN{c,OjR4M{mwNVM9N2F-
U2OS M3H6fmFxd3C2b4Ppd{BCe3OjeR?= NGW0TGwyOG2P Mkm3NlRp MoLzbY51\W6|aX\p[ZMhfGinIHfyc5d1cCCrbnjpZol1cW:wIH;mJJRp\SCXMl;TJINmdGy|IHnu[JVk\WRiYomgSI95 NX32bHZORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N|kxOTNpPkK0OlM6ODF|PD;hQi=>
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A2780cp MWnBdI9xfG:|aYOgRZN{[Xl? MlvXNk42dU1? NGXVeIIycA>? NVfmb5BT\GSKMl:= MVvlcohidmOnczDjbZNxdGG2aX6tbY5lfWOnZDDj[YxtKGSnYYTo NIi2VnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEixO|k1Pid-MkS4NVc6PDZ:L3G+
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HT-29  NI\OXlVCeG:ydH;zbZMhSXO|YYm= NW\yc|lyOW2P NH7nZ5k1QGh? NEX6c5dFVVOR NFjmfVNmdmijbnPld{Bjd3K2ZYrvcYljNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh2MkG1PEc,OjR6NEKxOVg9N2F-
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NBL-W-S  NFH3d2VCeG:ydH;zbZMhSXO|YYm= NFX4OGQydU1? NX3XVZZyPmh? NXu1U4x[cW6lcnXhd4V{KGOnbHygZZBweHSxc3nzJIlv\HWlZXSgZpkhT0GQVD22NS=> M{fLTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{KzNlIzLz5{NUOyN|IzOjxxYU6=
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HUVECs Mm\0SpVv[3Srb36gRZN{[Xl? MnPONVBuVQ>? NFuybpgxNjWq MVLk[YNz\WG|ZYOgeIhmKEGJRT3CV2FqdmS3Y3XkJIF2fG:yaHHnfUBt\X[n MoDjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2Nki1PVIoRjJzNE[4OVkzRC:jPh?=
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DAOY Mn35dWhVWyCjc4PhfS=> MXnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhTEGRWTDj[Yxtew>? M3G0VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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アッセイ
Methods Test Index PMID
Western blot α-SMA / TGF-β / LC-3BI / LC-3B II / Beclin-1 / NF-κB p65 ; caspase-3 / caspase-9 / PARP ; VEGF ; APP / BACE1 / ADAM17 / Presenilin 1 / Presenilin 2 / Nicastrin / APH-1 / Pen-2 / LC3-1 / LC3-2 29296191 25891159 26316755
Immunofluorescence LC3 / Hif-α / COX2 29039446
Growth inhibition assay Cell viability 26934124
体内試験 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K). 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the subarachnoid hemorrhage (SAH) group. 3-Methyladenine pretreatment significantly aggravates neurological symptoms when compared with the SAH + vehicle group. Autophagy is decreased when 3-Methyladenine treatment is applied. Conversely, cleaved caspase-3 is markedly up-regulated in the SAH + 3-Methyladenine group. In line with the up-regulation of cleaved caspase-3 expression, the number of TUNEL-positive cells in the right cortex is significantly increased in the SAH + 3-Methyladenine group compared with the SAH + vehicle group. [5]

お薦めの試験操作(参考用のみ)

キナーゼ試験:[4]
  • Protein degradation assay:

    HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.

細胞試験:[2]
  • 細胞株:HeLa cell line
  • 濃度: 1-10 mM
  • 反応時間:24, 48 or 72 hours
  • 実験の流れ:Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer.
動物試験:[5]
  • 動物モデル:Adult male Sprague–Dawley rats weighing 300-350 g
  • 投薬量:400 nM
  • 投与方法:Intracerebral ventricular

溶解度 (25°C)

体外

化学情報

分子量 149.15
化学式

C6H7N5

CAS No. 5142-23-4
保管 3 years -20°C powder
Smiles CN1C=NC(=N)C2=C1N=CN2

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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よくある質問(FAQ)

質問1:
I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

回答
As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

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