Pimonidazole

別名：Ro 03-8799

製品コード：S9919 純度：99.52%

Pimonidazole (Ro 03-8799) is a novel hypoxia marker used for complementary study of tumor hypoxia and cell proliferation in cervical carcinoma.

CAS No. 70132-50-2

サイズ	価格(税別)	在庫状況
5mg	JPY 16200	国内在庫あり
25mg	JPY 44700	国内在庫あり

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

製品安全説明書

現在のバッチを見る： S991901 DMSO] 51 mg/mL] false] Ethanol] 51 mg/mL] false] Water] Insoluble] false 純度： 99.52%

99.52

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関連化合物ライブラリー	FDA-approved Drug Library Natural Product Library Bioactive Compound Library-I Bioactive Compound Library-Ⅱ Express-Pick Library	もっと見る

生物活性

製品説明	Pimonidazole (Ro 03-8799) is a novel hypoxia marker used for complementary study of tumor hypoxia and cell proliferation in cervical carcinoma.

In Vitro
In vitro	Pimonidazole has radio-sensitivity and recovery capacity from radiation-induced damage depend on p53 status of the tumor cell.<sup><a class="sref" href="#s_ref">[3]</a></sup>
細胞実験	細胞株	HMV-II human malignant melanoma cells
	濃度	0.5 mM
	反応時間	1–4 h
	実験の流れ	HMV-II cells are seeded by 2,000 cells per well in 96-well culture plates, allowed to adhere for 30 hr in a humidified atmosphere with 5% CO<sub>2</sub> at 37 C, and followed by incubation in a hypoxic condition of 100% N2 set in a glass desicator for 18 hr. The liposomal Pmz or free-Pmz is applied in 100 μL of degassed medium and incubated for 3 hr in a hypoxic condition of 100% N2 set in a glass desicator. After X-ray irradiation, the cells are incubated for 3 hr in a humidified atmosphere with 5% CO2 at 37 C. The cells are confirmed to survive according to either morphological observation or cell viability assay using the redox indicator WST-8.

In Vivo
In Vivo	Pimonidazole can overcome radiation resistance in hypoxiaby melanoma cells, which is enhanced by liposome modification.<sup><a class="sref" href="#s_ref">[2]</a></sup>
動物実験	動物モデル	Human head and neck cancer xenograft model mice (BALB/c athymic nude mice; inoculated with FaDu cells)
	投与量	200 mg/kg
	投与経路	IOCV

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
臨床試験 (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT03718650	Withdrawn	Pancreatic Cancer\|Resectable Pancreatic Cancer	H. Lee Moffitt Cancer Center and Research Institute\|Varian Medical Systems	April 1 2021	Early Phase 1
NCT03410420	Suspended	Aortic Aneurysm Thoracic	University of Maryland Baltimore	October 1 2018	Phase 1
NCT03262311	Completed	Cancer	Institute of Cancer Research United Kingdom	November 10 2017	Not Applicable
NCT02624115	Completed	Colon Rectal Cancer Adenocarcinoma	University Health Network Toronto	May 1 2016	--
NCT02095249	Active not recruiting	Prostate Cancer	University Health Network Toronto	September 2014	Not Applicable
NCT00374517	Withdrawn	Refractory Pulmonary Tuberculosis	National Institute of Allergy and Infectious Diseases (NIAID)\|International Tuberculosis Research Center\|National Institutes of Health Clinical Center (CC)	September 5 2006	Phase 2

参考
[1] M A Varia, et al. Gynecol Oncol. 1998 Nov;71(2):270-7. [2] Masaki Y, et al. PLoS One. 2016 Aug 31;11(8):e0161639. [3] Kato S,et al. J Nanosci Nanotechnol. 2012 Jun;12(6):4472-7.

参考

化学情報

分子量	254.29	化学式	C₁₁H₁₈N₄O₃
CAS No.	70132-50-2	SDF	--
Smiles	OC(CN1CCCCC1)C[N]2C=CN=C2[N+]([O-])=O
保管	3 years -20°C powder	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3 years -20°C powder	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1个月-20°Cin solvent

In vitro
Batch:

DMSO : 51 mg/mL ( (200.55 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Ethanol : 51 mg/mL

Water : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):