LMK-235

LMK-235 is a selective inhibitor of HDAC4 and HDAC5 with IC50 of 11.9 nM and 4.2 nM, respectively.

LMK-235化学構造

CAS No. 1418033-25-6

サイズ 価格(税別) 在庫状況
10mM (1mL in DMSO) JPY 27600 国内在庫あり
JPY 24700 国内在庫あり
JPY 81100 国内在庫あり
JPY 160800 国内在庫なし(納期7~10日)

代表番号: 045-509-1970|電子メール:[email protected]
よく尋ねられる質問

文献中Selleckの製品使用例(35)

製品安全説明書

現在のバッチを見る: 純度: 99.94%
99.94

LMK-235関連製品

シグナル伝達経路

HDAC阻害剤の選択性比較

Cell Data

Cell Lines Assay Type Concentration Incubation Time 活性情報 PMID
DLD1 Function assay 1 to 10 uM 24 hrs Inhibition of VPS34 in human DLD1 cells assessed as prevention of p62 degradation at 1 to 10 uM incubated for 24 hrs by Western blot analysis 23252603
DLD1 Function assay 1 to 10 uM 24 hrs Inhibition of VPS34 in human DLD1 cells assessed as prevention of NCOA4 protein degradation at 1 to 10 uM incubated for 24 hrs by Western blot analysis 23252603
DLD1 Function assay 1 to 10 uM 24 hrs Inhibition of VPS34 in human DLD1 cells assessed as prevention of NBR1 protein degradation at 1 to 10 uM incubated for 24 hrs by Western blot analysis 23252603
DLD1 Function assay 1 to 10 uM 24 hrs Inhibition of VPS34 in human DLD1 cells assessed as prevention of NDP52 protein degradation at 1 to 10 uM incubated for 24 hrs by Western blot analysis 23252603
DLD1 Function assay 1 to 10 uM 24 hrs Inhibition of VPS34 in human DLD1 cells assessed as prevention of FTH1 protein degradation at 1 to 10 uM incubated for 24 hrs by Western blot analysis 23252603
A2780 Function assay 18 hrs Inhibition of HDAC in cisplatin resistant human A2780 cells after 18 hrs by fluorescence assay, IC50=0.32μM 23252603
A2780 Cytotoxicity assay 72 hrs Cytotoxicity against cisplatin resistant human A2780 cells after 72 hrs by MTT assay, IC50=0.32μM 23252603
A2780 Cytotoxicity assay 72 hrs Cytotoxicity against human A2780 cells after 72 hrs by MTT assay, IC50=0.49μM 23252603
A2780 Function assay 18 hrs Inhibition of HDAC in human A2780 cells after 18 hrs by fluorescence assay, IC50=0.65μM 23252603
KYSE-510 Function assay 18 hrs Inhibition of HDAC in cisplatin sensitive human KYSE-510 cells after 18 hrs by fluorescence assay, IC50=1μM 23252603
CAL27 Cytotoxicity assay 72 hrs Cytotoxicity against cisplatin sensitive human CAL27 cells after 72 hrs by MTT assay, IC50=1.03μM 23252603
MDA-MB-231 Cytotoxicity assay 72 hrs Cytotoxicity against cisplatin sensitive human MDA-MB-231 cells after 72 hrs by MTT assay, IC50=1.37μM 23252603
MDA-MB-231 Cytotoxicity assay 72 hrs Cytotoxicity against cisplatin resistant human MDA-MB-231 cells after 72 hrs by MTT assay, IC50=1.68μM 23252603
CAL27 Cytotoxicity assay 72 hrs Cytotoxicity against cisplatin resistant human CAL27 cells after 72 hrs by MTT assay, IC50=1.81μM 23252603
KYSE-510 Cytotoxicity assay 72 hrs Cytotoxicity against cisplatin resistant human KYSE-510 cells after 72 hrs by MTT assay, IC50=2.48μM 23252603
KYSE-510 Cytotoxicity assay 72 hrs Cytotoxicity against cisplatin sensitive human KYSE-510 cells after 72 hrs by MTT assay, IC50=2.96μM 23252603
HepG2 Antimalarial assay 48 hrs Antimalarial activity against exo-erythrocytic form of Plasmodium berghei infected in human HepG2 cells after 48 hrs, IC50=0.16μM 24904967
HepG2 Cytotoxicity assay 48 hrs Cytotoxicity against human HepG2 cells after 48 hrs, IC50=1.26μM 24904967
他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

生物活性

製品説明 LMK-235 is a selective inhibitor of HDAC4 and HDAC5 with IC50 of 11.9 nM and 4.2 nM, respectively.
Targets
HDAC5 [1]
(Cell-free assay)
HDAC4 [1]
(Cell-free assay)
4.2 nM 11.9 nM
In Vitro
In vitro

LMK-235 causes HDAC inhibition with IC50 of <1 μM in human cancer cell lines with different sensitivity towards cisplatin. In breast cancer cell line MDA-MB-231, tongue cancer cell line Cal27, and esophagus cell line Kyse510 cell line, LMK-235 displays a high cytotoxicity, and markedly enhances the cytotoxicity of cisplatin. [1]

In addition, LMK-235 also shows nanomolar activity against multiple malaria parasite life cycle stages. [2]

Kinase Assay HDAC IC50 Profiling
The in vitro inhibitory activity of compounds against seven human HDAC isoforms (1, 2, 4 C2A, 5 C2A, 6, 8, and 11) are performed with a fluorescent based assay according to the company’s standard operating procedure. The IC50 values are determined using 10 different concentrations with 3-fold serial dilution starting at 10 μM. TSA and vorinostat are used as reference compounds.
細胞実験 細胞株 A2780, Cal27, Kyse510, and MDA-MB-231 cell lines
濃度 ~10 μM
反応時間 72 hours
実験の流れ

The rate of cell survival under the action of test substances is evaluated by an improved MTT assay. The assay is based on the ability of viable cells to metabolize yellow MTT to violet formazan that can be detected spectrophotometrically. In brief, A2780, Cal27, Kyse510, and MDA-MB-231 cell lines are seeded at a density of 5000, 7000, 8000, and 10 000 cells/well in 96-well plates. After 24 h, cells are exposed to increased concentrations of the test compounds. Incubation is ended after 72 h, and cell survival is determined by addition of MTT solution (5 mg/mL in phosphate buffered saline). The formazan precipitate is dissolved in DMSO. Absorbance s measured at 544 and 690 nm in a FLUOstar microplate reader.

In Vivo
In Vivo

LMK-235 is a selective inhibitor of HDAC4.

動物実験 動物モデル C57BL/6-BALB/c mice
投与量 20 mg/kg
投与経路 i.p.

化学情報

分子量 294.35 化学式

C15H22N2O4

CAS No. 1418033-25-6 SDF Download LMK-235 SDFをダウンロードする
Smiles CC1=CC(=CC(=C1)C(=O)NOCCCCCC(=O)NO)C
保管

In vitro
Batch:

DMSO : 59 mg/mL ( (200.44 mM); 吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Ethanol : 59 mg/mL

Water : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量 濃度 体積 分子量

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

大学・企業名を記入してください
名前を記入してください
電子メール・アドレスを記入してください 有効なメールアドレスを入力してください
お問い合わせ内容をご入力ください
Tags: LMK-235を買う | LMK-235 ic50 | LMK-235供給者 | LMK-235を購入する | LMK-235費用 | LMK-235生産者 | オーダーLMK-235 | LMK-235化学構造 | LMK-235分子量 | LMK-235代理店