Trichostatin A (TSA)

製品コード:S1045

For research use only.

Trichostatin A (TSA) is an HDAC inhibitor with IC50 of ~1.8 nM in cell-free assays.

Trichostatin A (TSA)化学構造

CAS No. 58880-19-6

サイズ 価格(税別)
JPY 43000
JPY 90800
JPY 180100

代表番号: 03-5615-9297|電子メール:[email protected]
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文献中Selleckの製品使用例(220)

製品安全説明書

HDAC阻害剤の選択性比較

相関HDAC製品

生物活性

製品説明 Trichostatin A (TSA) is an HDAC inhibitor with IC50 of ~1.8 nM in cell-free assays.
ターゲット
HDAC [1]
(Cell-free assay)
~1.8 nM
体外試験

Trichostatin A inhibits the proliferation of eight breast carcinoma cell lines including MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3 with mean IC50 of 124.4 nM (range, 26.4-308.1 nM), with more potency against cell lines that express ERα than the ERα-negative cell lines. Trichostatin A inhibits HDAC activity similarly in all the breast cancer cell lines with mean IC50 of 2.4 nM (range, 0.6-2.6 nM), and results in pronounced histone H4 hyperacetylation. [1] Unlike Trapoxin (TPX) and Chlamydocin which potently inhibit HDAC1 or HDAC4 but not HDAC6, Trichostatin A inhibits these HDACs to a similar extent with IC50 of 6 nM, 38 nM, and 8.6 nM, respectively. [2] Trichostatin A (100 ng/mL) treatment induces the expression of transforming growth factor β type II receptor (TβRII) in MIA PaCa-2 cells through the recruitment of p300 and PCAF into a Sp1-NF-Y HDAC complex that binds the DNA element of TβRII promoter, which is associated with a concomitant acetylation of Sp1 and an overall decrease in the amount of HDAC associated with the complex. [4]

細胞データ
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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HeLa M4[0ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYLybZpOOjBibl2= M4\HXlczKGh? NHvsfXhFVVOR NWLH[WJSUUN3MDDv[kAzOG6P MmH4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzNkW3OFgoRjJ|MU[1O|Q5RC:jPh?=
HeLa M3\IfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVrlb|YzPDBibl2= NEnUWYQ1QCCq MlvkSG1UVw>? MlPMTWM2OCCxZjC0NI5O M1Tj[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MU[1O|Q5Lz5{M{G2OVc1QDxxYU6=
HeLa M1;NZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWHQfXNoOTByIH7N M12wXVI1KGh? Mke4SG1UVw>? MkDBTWM2OCCxZjCxNFBvVQ>? NHfLOlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G2OVc1QCd-MkOxOlU4PDh:L3G+
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HTK M1;xfmZ2dmO2aX;uJGF{e2G7 NXL3N4hvOTByLUiwNI5O NFfOVZg4OiCq M1rwU4Jtd2OtczDUS2Yu|rMkgKPJcoR2[2WmIGLPV{BidmRiSELPNuKhSWOldX31cIF1cW:w NH7tW5A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K4OFAxOid-MkOyPFQxODJ:L3G+
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HEK293 M{Ow[2Z2dmO2aX;uJGF{e2G7 M4XaPFEhyrWPwrC= NGHxVo8yQCCqwrC= MV\pcohq[mm2czD0bIUhc2GuaYLpck04NW2nZHnheIVlKHKnY4L1bZRu\W62IH;mJJN6dnCqaXzpck0yKGGpZ4Ll[4F1\XNiaX70c{Bi\2e{ZYPvcYV{ NUnXcYttRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyPFQ5PDhpPkKzNlg1QDR6PD;hQi=>
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HT29  Ml3GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVixPFAhdk1? MlOwO|IhcA>? MlfSTWM2OCCxZjCxPFAhdk1? NGTxRlY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2PFI3PSd-MkSzOlgzPjV:L3G+
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ACP02 M3;EOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDseIwzPTBxM{WwM|UxOG6P NFvMVZYzPGh? NFXO[nFl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgc4Yh[XCycn;4bY1ifGWueTC3NOKhLSCjdDCyOVDDqG6P NETlOWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[2PFU1Pyd-MkS2Olg2PDd:L3G+
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SKOV-3 NGfvOYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33kclEuOTEQvF2= NXXmN3JWOjSq NUi3T3lDTE2VTx?= NXfKRpFQUUN3MDDv[kA2NjcQvF2= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF4OUS5NUc,OjVzNkm0PVE9N2F-
MCF7 MmjpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIe4SpUxNTZyMH7N MkDVNlRp NIi5fVlFVVOR NY\mVJZZUUN3MDDv[kA4PW6P NXq2SI4{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxPVI4OjFpPkK1NVkzPzJzPD;hQi=>
MDA-MB-231 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3q1WFAuPjBybl2= NHr2fngzPGh? Mn3lSG1UVw>? M120VWlEPTBib3[gNVAxdk1? NULlXHB1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxPVI4OjFpPkK1NVkzPzJzPD;hQi=>
A431 MV3GeY5kfGmxbjDBd5NigQ>? MVW1NI5O MlfiNk83NzF{L{K0bC=> NXrTRmV4TE2VTx?= NGT5TJVi[3SrdnH0[ZMheDJzIHHu[EBqdmirYnn0d{BCXEZ|IHX4dJJme3Orb36= MmHGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|N{GwOlkoRjJ3M{exNFY6RC:jPh?=
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PC3  M3L2PGZ2dmO2aX;uJGF{e2G7 NHvJd5UxNjIQvF2= MkTqOFhp M4jqemROW09? MlzJbY5lfWOnczDpcoNz\WG|ZTDv[kBJTEGFMTDhcoQhUESDQ{Kgc44hW2y3ZzDn[Y5meyCycn;tc5Rmeg>? NUP6bZFWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0N|Q6QTdpPkK1OFM1QTl5PD;hQi=>
SW480  NW\ES|dCTnWwY4Tpc44hSXO|YYm= M2PTbVAvOc7:TR?= NIDrbGk1QGh? MmK3SG1UVw>? MXjpcoR2[2W|IHnuZ5Jm[XOnIH;mJGhFSUNzIHHu[EBJTEGFMjDvckBUdHWpIHflcoV{KHC{b33veIVz Mle4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{S5PVcoRjJ3NEO0PVk4RC:jPh?=
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melanoma B16/BL6 MoCySpVv[3Srb36gZZN{[Xl? M3zZPWlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpcXO2b37lJIRm[WOndInsZZNmeyBqSFTBR|EqKHC{ZYDhdoVlKG[{b32gcY92e2VibXXsZY5wdWFiQkG2M2JNPiClZXzsd{whUUN3MDC9JFAvODF7IN88UU4> NVizUphPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUWxNFk3OjZpPkG1NVA6PjJ4PD;hQi=>
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melanoma B16/BL6 M17PS2Z2dmO2aX;uJIF{e2G7 M37mc2lvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpcXO2b37lJIRm[WOndInsZZNmKDZicILldIFz\WRiZoLvcUBud3W|ZTDt[Yxidm:vYTDCNVYwSkx4IHPlcIx{NCCLQ{WwJF0hOC5yMkig{txONg>? MlTCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTVzMEm2NlYoRjF3MUC5OlI3RC:jPh?=
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293T NGS4dpVHfW6ldHnvckBie3OjeR?= Mn3pTY5pcWKrdHnvckBw\iCqdX3hckBJTEGFNDDlfJBz\XO|ZXSgbY4hOjl|VDDj[YxteyxiSVO1NEA:KDBwMECyJO69VS5? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh6MUKzNkc,OTd6OEGyN|I9N2F-
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293T NVHaZlZqTnWwY4Tpc44h[XO|YYm= NF:1N5BKdmirYnn0bY9vKG:oIFjERWMyKGW6cILld5Nm\CCrbjCyPVNVKGOnbHzzMEBKSzVyIE2gNE4yQSEQvF2u NWLUU49pRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5NFA6OTFpPkG3PVAxQTFzPD;hQi=>
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293T M3LQ[mZ2dmO2aX;uJIF{e2G7 M3zDVGlvcGmkaYTpc44hd2ZiSFTBR|Yh\XiycnXzd4VlKGmwIEK5N3Qh[2WubIOsJGlEPTBiPTCwMlI5KM7:TT6= NHrOcYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{mwNFkyOSd-MUe5NFA6OTF:L3G+
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PC12 MlfuSpVv[3Srb36gZZN{[Xl? Mk\LNVAhfU1? NVXt[GFpPDhiaILz NIP6fY9KdmirYnn0bY9vKG:oIFjERWM3KGmwIILheEBRSzF{IHPlcIx{KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBidHCqYT30eYJ2dGmwIHHj[ZR6dGG2aX;uJIF1KDFyIIXNJIFnfGW{IES4JIhzeyCkeTDX[ZN1\XKwIHLsc5R1cW6pIHHuZYx6e2m| M3rLT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUC1OlgxLz5{M{mwOVY5ODxxYU6=
PC12 NFzNVmJHfW6ldHnvckBie3OjeR?= MV2xNEB2VQ>? MkjBOUBl[Xm| M3;1U2lv\HWldHnvckBw\iCwZYXybZRmKG:3dHfyc5d1cCCrbjDyZZQhWENzMjDj[YxteyCjdDCxNEB2VSCjZoTldkA2KGSjeYO= NXLvcnJuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFU3QDBpPkKzPVA2PjhyPD;hQi=>
SH-SY5Y M4L3fGZ2dmO2aX;uJIF{e2G7 MUGxNEB2VQ>? NUnXbpByPSCmYYnz MmTETY5lfWO2aX;uJI9nKG6ndYLpeIUhd3W2Z4Lve5RpKGmwIHj1cYFvKFOKLWPZOXkh[2WubIOgZZQhOTBidV2gZYZ1\XJiNTDkZZl{ MoCwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEW2PFAoRjJ|OUC1OlgxRC:jPh?=
A549 MYPBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NVLTTGJmOC52IIXN M3zPS|I1KGi{cx?= NXixNVBCSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIF{e2W|c3XkJIF{KHOqcoXub4VvKGGwZDDzbIFzeCC|aHHw[UBifCByLkSgeW0h[W[2ZYKgNlQhcHK|IHL5JJBp[XOnIHPvcpRz[XO2IH3pZ5Jwe2OxcIm= NHWyeJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4OFEyOid-MkW4PFQyOTJ:L3G+
HeLa MmHzSpVv[3Srb36gZZN{[Xl? M2DyWWlvcGmkaYTpc44hd2ZiSFTBR|YhcW5iaIXtZY4hUGWOYTDj[YxteyCjc4Pld5Nm\CCjczD0eYJ2dGmwIHHj[ZR6dGG2aX;uJIlv[3WkYYTl[EBnd3Jib4\ldo5q\2i2IHL5JIludXWwb3\seY9z\XOlZX7j[UBucWO{b4Pjc5Bq[yCjbnHsfZNqew>? M1G4XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NkGxPVE6Lz5{Nk[xNVkyQTxxYU6=
C127-LT NEPTeFhHfW6ldHnvckBie3OjeR?= NHvsdWgzODBibl2= MWWyOEBpenN? MorGTY5pcWKrdHnvckBw\iCKRFHDJIlvKG2xdYPlJGMyOjdvTGSgZ4VtdHNiYYPz[ZN{\WRiYYOgZYN1cX[jdHnvckBw\iCHR1\QJIV5eHKnc4Ppc44h[XRiMkCwJI5OKGGodHXyJFI1KGi{czDifUBndHWxcnXzZ4Vv[2VibXnjdo9{[2:yeR?= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjl7NkO3Nkc,OjZ7OU[zO|I9N2F-
HeLa nuclear NUXsSotQTnWwY4Tpc44h[XO|YYm= NES0ZYUyOCC2bzCyOUB2VQ>? MnH4N|AhdWmwcx?= MWXDc41x\XSrdHn2[UBqdmirYnn0bY9vKG:oIFjERWMhcW5iaIXtZY4hUGWOYTDueYNt\WG{IHPlcIx{KGG2IEGwJJRwKDJ3IIXNJJV{cW6pIFLvZ{1i[2W2eXytcJl{cW6nLVHNR{BieyC|dXLzeJJifGViYX\0[ZIhOzBibXnud{BjgSCOaX7le4VifmW{LVL1dosheGyxdDDhcoFtgXOrcx?= NW\t[|ZzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5PVY{PzJpPkK2PVk3Ozd{PD;hQi=>
HeLa NVzOS4xwTnWwY4Tpc44h[XO|YYm= NXvMTmhxOiC3TR?= NIfwR5EzPCCqcoO= NH3IVVhKdmirYnn0bY9vKG:oIFjERWMyKGmwIHj1cYFvKEinTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgbJlx\XKjY3X0fYxifGmxbjDv[kBpcXO2b37lJGg{KGG2IEKgeW0hcW6ldXLheIVlKG[xcjCyOEBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ MoPFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyNkC3OlQoRjJ5ME[wO|Y1RC:jPh?=
HN13 MlPrSpVv[3Srb36gZZN{[Xl? M1K3VlI1KGi{cx?= MV\Jcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gTG4yOyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gbIl{fG:wZTDIN{BNgXN7IHHj[ZR6dGG2aX;uJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IHntcZVvd2[udX;y[ZNk\W6lZTDhd5NigQ>? NUnyblRyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmwN|ExPjZpPkK5NFMyODZ4PD;hQi=>
HeLaS3 NVHjT4plTnWwY4Tpc44h[XO|YYm= NVL2XnhZOTBidV2= M4XRcmlvcGmkaYTpc44hd2ZiSFTBR{BqdiCqdX3hckBJ\UyjU{OgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hcGm|dH;u[UBJO0t7IHHj[ZR6dGG2aX;uJIF1KDFyIIXNJI1m[XO3cnXkJIludWWmaXH0[Yx6KGK7IHntcZVvd2Kub4SgZY5idHm|aYO= NIrkXVg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEK1PFU{PSd-M{CyOVg2OzV:L3G+
HeLaS3 MWDGeY5kfGmxbjDhd5NigQ>? Ml7tNVAhfU1? MY[xOkBpenN? MVTJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gTIVN[VN|IHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFNMQSCjY3X0fYxifGmxbjDheEAyOCC3TTD0doVifGWmIH\vdkAyPiCqcoOg[o9tdG:5ZXSgZpkh[2:vcH;1coQhf2G|aH;1eEBidmRibXXhd5Vz\WRidYCgeI8hOjRiaILzJIJ6KGmvbYXuc4Jtd3RiYX7hcJl{cXN? M{DXTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMkW4OVM2Lz5|MEK1PFU{PTxxYU6=
アッセイ
Methods Test Index PMID
Western blot EGFR / STAT3 ; Cyclin D1 / CDK6 / CDK4 / p-RB / RB ; MMP-1 / MMP-3 / MMP-13 / TIMP-1 ; Acetyl-H3 (Lys27) / H3 (96C10) / Acetyl-H4(Lys8) / H4(L64C1) ; α-H3Ac / α-H4Ac / α-H3K9Ac / α-H3K9 S10 / α-H3K4 me / α-H3K4 me2 / α-H3K9 me2 31289542 27431944 23802098
Immunofluorescence α-C/EBPβ / α-HP1α ; α-HDAC1 ; α-tubulin / acetylated histone 3 21122806 29045501 27957719
Growth inhibition assay Cell viability (A549 cells) ; Cell viability (MDA-MB-231 cells) ; Cell viability (PC3 cells) 27571418 27548148 31289542
体内試験 Administration of Trichostatin A at 0.5 mg/kg for 4 weeks displays potent antitumor activity in the N-methyl-N-nitrosourea carcinogen-induced rat mammary carcinoma model, without any measurable toxicity at doses up to 5 mg/kg. [1] Single intraperitoneal doses of 10 mg/kg Trichostatin A in nontransgenic and spinal muscular atrophy (SMA) model mice results in increased levels of acetylated H3 and H4 histones and modest increases in survival motor neuron (SMN) gene expression. Administration of Trichostatin A at 10 mg/kg/day improves survival, attenuates weight loss, and enhances motor behavior in the SMA model mice. [5]

お薦めの試験操作(参考用のみ)

キナーゼ試験:

[1]

  • In vitro HDAC activity:

    Total cellular extracts are prepared from each breast cancer cell line (MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, or SK-BR-3). A 20 μL crude cell extract (~2.5 ×105 cells), in the presence of varying concentrations of Trichostatin A in 0.1% (v/v) ethanol or 0.1% (v/v) ethanol as vehicle control, are incubated for 60 minutes at 25 °C with 1 μL (~1.5 × 106 cpm) of [3H]acetyl-labeled histone H4 peptide substrate (NH2-terminal residues 2-20) that has been acetylated with [3H]acetic acid, sodium salt (3.7 GBq/mmol) by an in vitro incorporation method. Each 200 μL reaction is quenched with 50 μL of 1 M HCl/0.16 M acetic acid and extracted with 600 μL of ethyl acetate, and released [3H]acetate is quantified by scintillation counting. IC50 values are determined graphically using nonlinear regression to fit inhibition data to the appropriate dose-response curve.

細胞試験:

[1]

  • 細胞株:MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, and SK-BR-3
  • 濃度: Dissolved in absolute ethanol, final concentrations ~10 μM
  • 反応時間:96 hours
  • 実験の流れ:

    Cells are exposed to various concentrations of Trichostatin A for 96 hours. After treatment, cell proliferation is estimated using the sulforhodamine B colorimetric assay. Cell viability is determined by trypan blue exclusion.

動物試験:

[1]

  • 動物モデル:Inbred virgin female (Ludwig/Wistar/Olac) rats bearing tumors induced with NMU
  • 投薬量:~5 mg/kg/day
  • 投与方法:Injection s.c.

溶解度 (25°C)

体外

体内

左から(NMPから)右の順に溶剤を製品に加えます(文献ではなく、Selleckの実験によるデータ):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
混合させたのち直ちに使用することを推奨します。

1.5mg/mL

化学情報

分子量 302.4
化学式

C17H22N2O3

CAS No. 58880-19-6
Storage 3年 -20°C
2年 -80°C in solvent
Smiles CC(C=C(C)C=CC(=O)NO)C(=O)C1=CC=C(C=C1)N(C)C

投与溶液組成計算機(クリア溶液)

ステップ1:実験データを入力してください。(実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します)

mg/kg g μL

ステップ2:投与溶媒の組成を入力してください。(ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください)

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果:

投与溶媒濃度: mg/ml;

DMSOストック溶液調製方法: mg 試薬を μL DMSOに溶解する(濃度 mg/mL, 注:濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法:Take μL DMSOストック溶液に μL PEG300,を加え、完全溶解後μL Tween 80,を加えて完全溶解させた後 μL ddH2O,を加え完全に溶解させます。

投与溶媒調製方法:μL DMSOストック溶液に μL Corn oil,を加え、完全溶解。

注意:1.ストック溶液に沈殿、混濁などがないことをご確認ください;
2.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

便利ツール

質量 濃度 体積 分子量

臨床試験

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05414890 Recruiting Diagnostic Test: CBA-TSA NMO Spectrum Disorder|NMDA-R Encephalitis|Diagnostic Self Evaluation|Immune System Diseases|Nervous System Diseases|Autoimmune Diseases of the Nervous System|Autoimmune Diseases Beijing Tiantan Hospital|Tianjin Medical University General Hospital June 30 2022 --
NCT05271955 Not yet recruiting Device: e-GOLIAH|Behavioral: Usual care Autism Spectrum Disorder Assistance Publique - Hôpitaux de Paris|University Hospital Montpellier June 15 2022 Not Applicable
NCT05355363 Not yet recruiting Diagnostic Test: Standard Colonoscopy Colorectal Cancer|Colon Adenoma Centre hospitalier de l''Université de Montréal (CHUM) May 1 2022 Not Applicable
NCT05219097 Recruiting Diagnostic Test: CBA Assay Diagnostic Self Evaluation|Myasthenia Gravis Tianjin Medical University General Hospital January 2022 --
NCT04606771 Recruiting Drug: Osimertinib + Savolitinib|Drug: Savolitinib + Placebo Non-Small Cell Lung Cancer AstraZeneca September 28 2020 Phase 2
NCT03901053 Recruiting Device: Reaktiv AFO|Device: PhatBrace AFO Foot Injuries and Disorders Jason Wilken|Minneapolis Veterans Affairs Medical Center|Walter Reed National Military Medical Center|Henry M. Jackson Foundation for the Advancement of Military Medicine|Center for Veterans Research and Education|University of Delaware|Johns Hopkins Bloomberg School of Public Health|University of Iowa February 27 2020 Not Applicable

(data from https://clinicaltrials.gov, updated on 2022-08-01)

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

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よくある質問(FAQ)

質問1:
I would like to obtain the enantiomers of TSA, as separate chemicals: R-TSA and S-TSA. Do you have any ideas?

回答
Our S1045 Trichostatin A (TSA) is R enantiomer.

Tags: Trichostatin A (TSA)を買う | Trichostatin A (TSA) ic50 | Trichostatin A (TSA)供給者 | Trichostatin A (TSA)を購入する | Trichostatin A (TSA)費用 | Trichostatin A (TSA)生産者 | オーダーTrichostatin A (TSA) | Trichostatin A (TSA)化学構造 | Trichostatin A (TSA)分子量 | Trichostatin A (TSA)代理店